Q28969
SS
Gene name |
NOS3 (NOS) |
Protein name |
Nitric oxide synthase 3 |
Names |
EC 1.14.13.39 , Constitutive NOS , cNOS , EC-NOS , NOS type III , NOSIII , Nitric oxide synthase, endothelial , Endothelial NOS , eNOS |
Species |
Sus scrofa (Pig) |
KEGG Pathway |
ssc:397557 |
EC number |
1.14.13.39: With NADH or NADPH as one donor, and incorporation of one atom of oxygen |
Protein Class |
|
Descriptions
Nitric oxide synthase produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. The primary sequences of the three mammalian nitric oxide synthase (NOS) isoforms differ by the insertion of a 52-55-amino acid loop into the reductase domains of the endothelial (eNOS) and neuronal (nNOS), but not inducible (iNOS). The loop mediates the autoinhibition in eNOS and nNOS. Deletion of the autoinhibitory loop increases the catalytic activity of eNOS.
Autoinhibitory domains (AIDs)
Target domain |
122-483 (NO synthase domain);732-1164 (FAD/NAD-binding domain) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
1 structures for Q28969
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q28969-F1 | Predicted | AlphaFoldDB |
No variants for Q28969
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for Q28969 | |||||
No associated diseases with Q28969
17 regional properties for Q28969
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Flavodoxin-like | 523 - 536 | IPR001094-1 |
| domain | Flavodoxin-like | 570 - 581 | IPR001094-2 |
| domain | Flavodoxin-like | 646 - 656 | IPR001094-3 |
| domain | Flavodoxin-like | 670 - 689 | IPR001094-4 |
| domain | Oxidoreductase FAD/NAD(P)-binding | 1013 - 1125 | IPR001433 |
| domain | Flavoprotein pyridine nucleotide cytochrome reductase | 795 - 805 | IPR001709-1 |
| domain | Flavoprotein pyridine nucleotide cytochrome reductase | 940 - 947 | IPR001709-2 |
| domain | Flavoprotein pyridine nucleotide cytochrome reductase | 976 - 985 | IPR001709-3 |
| domain | Flavoprotein pyridine nucleotide cytochrome reductase | 1012 - 1031 | IPR001709-4 |
| domain | Flavoprotein pyridine nucleotide cytochrome reductase | 1042 - 1051 | IPR001709-5 |
| domain | Flavoprotein pyridine nucleotide cytochrome reductase | 1055 - 1066 | IPR001709-6 |
| domain | Flavoprotein pyridine nucleotide cytochrome reductase | 1087 - 1103 | IPR001709-7 |
| domain | Flavoprotein pyridine nucleotide cytochrome reductase | 1112 - 1120 | IPR001709-8 |
| domain | Sulfite reductase [NADPH] flavoprotein alpha-component-like, FAD-binding | 754 - 981 | IPR003097 |
| domain | Nitric oxide synthase, N-terminal | 122 - 483 | IPR004030 |
| domain | Flavodoxin/nitric oxide synthase | 522 - 705 | IPR008254 |
| domain | FAD-binding domain, ferredoxin reductase-type | 758 - 1004 | IPR017927 |
Functions
| Description | ||
|---|---|---|
| EC Number | 1.14.13.39 | With NADH or NADPH as one donor, and incorporation of one atom of oxygen |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
6 GO annotations of cellular component
| Name | Definition |
|---|---|
| caveola | A membrane raft that forms small pit, depression, or invagination that communicates with the outside of a cell and extends inward, indenting the cytoplasm and the cell membrane. Examples include flask-shaped invaginations of the plasma membrane in adipocytes associated with caveolin proteins, and minute pits or incuppings of the cell membrane formed during pinocytosis. Caveolae may be pinched off to form free vesicles within the cytoplasm. |
| cytoskeleton | A cellular structure that forms the internal framework of eukaryotic and prokaryotic cells. The cytoskeleton includes intermediate filaments, microfilaments, microtubules, the microtrabecular lattice, and other structures characterized by a polymeric filamentous nature and long-range order within the cell. The various elements of the cytoskeleton not only serve in the maintenance of cellular shape but also have roles in other cellular functions, including cellular movement, cell division, endocytosis, and movement of organelles. |
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| Golgi apparatus | A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
7 GO annotations of molecular function
| Name | Definition |
|---|---|
| calmodulin binding | Binding to calmodulin, a calcium-binding protein with many roles, both in the calcium-bound and calcium-free states. |
| flavin adenine dinucleotide binding | Binding to FAD, flavin-adenine dinucleotide, the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes, in either the oxidized form, FAD, or the reduced form, FADH2. |
| FMN binding | Binding to flavin mono nucleotide. Flavin mono nucleotide (FMN) is the coenzyme or the prosthetic group of various flavoprotein oxidoreductase enzymes. |
| heme binding | Binding to a heme, a compound composed of iron complexed in a porphyrin (tetrapyrrole) ring. |
| metal ion binding | Binding to a metal ion. |
| NADP binding | Binding to nicotinamide-adenine dinucleotide phosphate, a coenzyme involved in many redox and biosynthetic reactions; binding may be to either the oxidized form, NADP+, or the reduced form, NADPH. |
| nitric-oxide synthase activity | Catalysis of the reaction |
7 GO annotations of biological process
| Name | Definition |
|---|---|
| arginine catabolic process | The chemical reactions and pathways resulting in the breakdown of arginine, 2-amino-5-(carbamimidamido)pentanoic acid. |
| negative regulation of blood pressure | Any process in which the force of blood traveling through the circulatory system is decreased. |
| nitric oxide biosynthetic process | The chemical reactions and pathways resulting in the formation of nitric oxide, nitrogen monoxide (NO), a colorless gas only slightly soluble in water. |
| nitric oxide mediated signal transduction | Any intracellular signal transduction in which the signal is passed on within the cell via nitric oxide (NO). Includes synthesis of nitric oxide, receptors/sensors for nitric oxide (such as soluble guanylyl cyclase/sGC) and downstream effectors that further transmit the signal within the cell. Nitric oxide transmits its downstream effects through either cyclic GMP (cGMP)-dependent or independent mechanisms. |
| response to hormone | Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a hormone stimulus. |
| response to lipopolysaccharide | Any process that results in a change in state or activity of an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a lipopolysaccharide stimulus; lipopolysaccharide is a major component of the cell wall of gram-negative bacteria. |
| vasodilation | An increase in the internal diameter of blood vessels, especially arterioles or capillaries, due to relaxation of smooth muscle cells that line the vessels, and usually resulting in a decrease in blood pressure. |
11 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P29473 | NOS3 | Nitric oxide synthase, endothelial | Bos taurus (Bovine) | EV |
| Q9TUX8 | NOS3 | Nitric oxide synthase 3 | Canis lupus familiaris (Dog) (Canis familiaris) | SS |
| Q9UBK8 | MTRR | Methionine synthase reductase | Homo sapiens (Human) | PR |
| P29475 | NOS1 | Nitric oxide synthase, brain | Homo sapiens (Human) | SS |
| P29474 | NOS3 | Nitric oxide synthase, endothelial | Homo sapiens (Human) | SS |
| Q8C1A3 | Mtrr | Methionine synthase reductase | Mus musculus (Mouse) | PR |
| P70313 | Nos3 | Nitric oxide synthase 3 | Mus musculus (Mouse) | SS |
| Q498R1 | Mtrr | Methionine synthase reductase | Rattus norvegicus (Rat) | PR |
| P29476 | Nos1 | Nitric oxide synthase, brain | Rattus norvegicus (Rat) | SS |
| Q62600 | Nos3 | Nitric oxide synthase 3 | Rattus norvegicus (Rat) | SS |
| Q9FKW6 | LFNR1 | Ferredoxin--NADP reductase, leaf isozyme 1, chloroplastic | Arabidopsis thaliana (Mouse-ear cress) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MGNLKSVGQE | PGPPCGLGLG | LGLGLCGKQG | PATPAPEPSR | APAPATPHAP | EHSPAPNSPT |
| 70 | 80 | 90 | 100 | 110 | 120 |
| LTRPPEGPKF | PRVKNWEVGS | ITYDTLCAQS | QQDGPCTPRR | CLGSLVLPRK | LQSRPSPGPP |
| 130 | 140 | 150 | 160 | 170 | 180 |
| PAEQLLSQAR | DFINQYYSSI | KRSGSQAHEE | RLQEVEAEVA | TTGTYHLGES | ELVFGAKQAW |
| 190 | 200 | 210 | 220 | 230 | 240 |
| RNAPRCVGRI | QWGKLQVFDA | RDCSSAQEMF | TYICNHIKYA | TNRGNLRSAI | TVFPQRTPGR |
| 250 | 260 | 270 | 280 | 290 | 300 |
| GDFRIWNSQL | VRYAGYRQQD | GSVRGDPANV | EITELCIQHG | WTPGNGRFDV | LPLLLQAPDE |
| 310 | 320 | 330 | 340 | 350 | 360 |
| PPELFALPPE | LVLEVPLEHP | TLEWFAALGL | RWYALPAVSN | MLLEIGGLEF | PAAPFSGWYM |
| 370 | 380 | 390 | 400 | 410 | 420 |
| STEIGTRNLC | DPHRYNILED | VAVCMDLDTR | TTSSLWKDKA | AVEINLAVLH | SYQLAKVTIV |
| 430 | 440 | 450 | 460 | 470 | 480 |
| DHHAATASFM | KHLENEQKAR | GGCPADWAWI | VPPISGSLTP | VFHQEMVNYV | LSPAFRYQPD |
| 490 | 500 | 510 | 520 | 530 | 540 |
| PWKGSAAKGT | GIARKKTFKE | VANAVKISAS | LMATVMPKRV | KASILYASET | VRAQSYAQQL |
| 550 | 560 | 570 | 580 | 590 | 600 |
| GRLFRKAFDP | RVLCMDEYDV | VSLEHETLVL | VVTSTFGNGD | PPENGESFAA | ALMEMSGPYN |
| 610 | 620 | 630 | 640 | 650 | 660 |
| GSPRPEQHRS | YKIRFNSVSC | SDPLVSSWRR | KRKESSNTDS | AGALGTLRFC | VFGLGSRAYP |
| 670 | 680 | 690 | 700 | 710 | 720 |
| HFCAFARAVD | TRLEELGGER | LLQLGQGDEL | CGQEEAFRGW | AQAAFQASCE | TFCVGEDAKA |
| 730 | 740 | 750 | 760 | 770 | 780 |
| AARDIFSPKR | SWKRQRYRLS | AQVEGLQLLP | GLVHVHRRKM | FQATVLSVEN | LQSSKSTRAT |
| 790 | 800 | 810 | 820 | 830 | 840 |
| ILVRLDTEGQ | EGLQYQPGDH | IGICPPNRTG | LVEALLSRVE | DPTPPTESVG | VEQLEKGSPG |
| 850 | 860 | 870 | 880 | 890 | 900 |
| GPPPSWVRDP | RLPPYTLRQA | LTFFLDITSP | PSPRLLRVLS | TLAEEPSEQQ | ELETLSQDPR |
| 910 | 920 | 930 | 940 | 950 | 960 |
| RYEEWKWFRC | PTLLEVLEQF | PSVALPTPLL | LTQLALLQPR | YYSVSSAPST | YPGEIHPTVA |
| 970 | 980 | 990 | 1000 | 1010 | 1020 |
| VLAYRTQDGL | GPLHYGVCST | WLGQLKPGDP | VPCFIRAAPS | FRLPPDPSLP | CILVGPGTGI |
| 1030 | 1040 | 1050 | 1060 | 1070 | 1080 |
| APFRGFWQER | LHDIESKGLQ | PAPMTLVFGC | RCSQLDHLYR | DEVQDAQQRG | VFGRVLTAFS |
| 1090 | 1100 | 1110 | 1120 | 1130 | 1140 |
| REPDSPKTYV | QDILRTELAA | EVHRVLCLER | GHMFVCGDVT | MATSVLQTVQ | RILATEGNME |
| 1150 | 1160 | 1170 | 1180 | 1190 | 1200 |
| LDEAGDVIGV | LRDQQRYHED | IFGLTLRTQE | VTSRIRTQSF | SLQERHLRGA | VPWTFDPPGP |
| DTPGP |