Q24368

SS
Gene name
Iswi (CG8625)
Protein name
Chromatin-remodeling complex ATPase chain Iswi
Names
EC 3.6.4.- , CHRAC 140 kDa subunit , Nucleosome-remodeling factor 140 kDa subunit , NURF-140 , Protein imitation swi
Species
Drosophila melanogaster (Fruit fly)
KEGG Pathway
dme:Dmel_CG8625
EC number
3.6.4.-: Acting on ATP; involved in cellular and subcellular movement
Protein Class

Descriptions

SMARCA5 (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5, SNF2h) is a helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and a catalytic subunit of ISWI chromatin-remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair. <br>A highly conserved acidic patch formed by histones H2A and H2B of the nucleosome relieves the autoinhibition imposed by the AutoN and the NegC regions of the SMARCA5. In the autoinhibited state, AutoN and NegC hold the remodeler in an inactive state. The active state is promoted by AutoN and NegC binding near the acidic patch and by H4 tail binding to the ATPase domain. Thus, the acidic patch helps promote the translocation competent state of SMARCA5 by providing a binding site for NegC and AutoN.

Autoinhibitory domains (AIDs)

86-97 (AutoN motif) SS

Target domain

131-586 (Helicase domain)

Relief mechanism

Partner binding

Assay

588-649 (NegC region) SS

Target domain

131-586 (Helicase domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

2 structures for Q24368

Entry ID Method Resolution Chain Position Source
1OFC X-ray 190 A X 691-991 PDB
AF-Q24368-F1 Predicted AlphaFoldDB

No variants for Q24368

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q24368

No associated diseases with Q24368

10 regional properties for Q24368

Type Name Position InterPro Accession
domain SNF2, N-terminal 131 - 410 IPR000330
domain SANT/Myb domain 796 - 845 IPR001005-1
domain SANT/Myb domain 898 - 962 IPR001005-2
domain Helicase, C-terminal domain-like 432 - 586 IPR001650
domain Helicase superfamily 1/2, ATP-binding domain 124 - 316 IPR014001
domain ISWI, HAND domain 697 - 796 IPR015194
domain SLIDE domain 853 - 966 IPR015195
domain SANT domain 795 - 847 IPR017884
domain Isw1/2, N-terminal 125 - 345 IPR044754
domain SNF2/RAD5-like, C-terminal helicase domain 430 - 556 IPR049730

Functions

Description
EC Number 3.6.4.- Acting on ATP; involved in cellular and subcellular movement
Subcellular Localization
  • Nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

9 GO annotations of cellular component

Name Definition
ACF complex An ISWI complex that contains an ATPase subunit of the ISWI family (SNF2H in mammals, Isw2 in S. cerevisiae), an ACF1 homolog, and generally no other subunits, though Xenopus is an exception with a third non-conserved subunit. ACF plays roles in regulation of RNA polymerase II transcription and in DNA replication and repair.
CHRAC An ISWI complex that contains an ATPase subunit of the ISWI family (SNF2H in mammals, Isw2 in S. cerevisiae), an ACF1 homolog, and additional small histone fold subunits (generally two of these, but Xenopus has only one and some additional non-conserved subunits). CHRAC plays roles in the regulation of RNA polymerase II transcription and in DNA replication and repair.
chromatin The ordered and organized complex of DNA, protein, and sometimes RNA, that forms the chromosome.
ISWI-type complex Any nuclear protein complex that contains an ATPase subunit of the imitation switch (ISWI) family. ISWI ATPases are involved in assembling chromatin and in sliding and spacing nucleosomes to regulate transcription of nuclear RNA polymerases I, II, and III and also DNA replication, recombination and repair.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
NURF complex An ISWI complex that contains an ATPase subunit of the ISWI family (SNF2L in mammals), a NURF301 homolog (BPTF in humans), and additional subunits, though the composition of these additional subunits varies slightly with species. NURF is involved in regulation of transcription from TRNA polymerase II promoters.
polytene chromosome A type of chromosome in a polyploid cell, formed when multiple copies of homologous chromosomes are aligned side by side to give a giant chromosome in which distinct chromosome bands are readily visible.
RSF complex An ISWI complex that contains an ATPase subunit of the ISWI family (SNF2H in mammals) and an RSF1 homolog. It mediates nucleosome deposition and generates regularly spaced nucleosome arrays. In mammals, RSF is involved in regulation of transcription from RNA polymerase II promoters).
transcription regulator complex A protein complex that is capable of associating with DNA by direct binding, or via other DNA-binding proteins or complexes, and regulating transcription.

12 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction
ATP-dependent activity, acting on DNA Catalytic activity that acts to modify DNA, driven by ATP hydrolysis.
ATP-dependent chromatin remodeler activity An activity, driven by ATP hydrolysis, that modulates the contacts between histones and DNA, resulting in a change in chromosome architecture within the nucleosomal array, leading to chromatin remodeling.
DNA binding Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
DNA-binding transcription factor binding Binding to a DNA-binding transcription factor, a protein that interacts with a specific DNA sequence (sometimes referred to as a motif) within the regulatory region of a gene to modulate transcription.
helicase activity Catalysis of the reaction
histone binding Binding to a histone, any of a group of water-soluble proteins found in association with the DNA of eukaryotic or archaeal chromosomes. They are involved in the condensation and coiling of chromosomes during cell division and have also been implicated in gene regulation and DNA replication. They may be chemically modified (methylated, acetlyated and others) to regulate gene transcription.
histone octamer slider activity A chromatin remodeler activity that slides core histone octamers along chromosomal DNA.
nucleosome array spacer activity A histone octamer slider activity that spaces nucleosomes along chromosomal DNA. This activity is involved in assembling chromatin in uniform nucleosome arrays to regulate transcription by RNA polymerases I, II, and III, as well as DNA replication, recombination and repair.
nucleosome binding Binding to a nucleosome, a complex comprised of DNA wound around a multisubunit core and associated proteins, which forms the primary packing unit of DNA into higher order structures.
nucleotide binding Binding to a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose.

15 GO annotations of biological process

Name Definition
chromatin organization The assembly or remodeling of chromatin composed of DNA complexed with histones, other associated proteins, and sometimes RNA.
chromatin remodeling A dynamic process of chromatin reorganization resulting in changes to chromatin structure. These changes allow DNA metabolic processes such as transcriptional regulation, DNA recombination, DNA repair, and DNA replication.
DNA replication-dependent chromatin assembly The formation of nucleosomes on newly synthesized DNA, coupled to strand elongation.
heterochromatin formation An epigenetic gene silencing mechanism in which chromatin is compacted into heterochromatin, resulting in a chromatin conformation refractory to transcription. This process starts with heterochromatin nucleation, its spreading, and ends with heterochromatin boundary formation.
negative regulation of DNA-templated transcription Any process that stops, prevents, or reduces the frequency, rate or extent of cellular DNA-templated transcription.
nuclear speck organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of nuclear specks, a class of nuclear body in which splicing factors are localized.
nucleosome organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of one or more nucleosomes.
positive regulation of DNA-templated transcription Any process that activates or increases the frequency, rate or extent of cellular DNA-templated transcription.
positive regulation of transcription by RNA polymerase II Any process that activates or increases the frequency, rate or extent of transcription from an RNA polymerase II promoter.
regulation of circadian rhythm Any process that modulates the frequency, rate or extent of a circadian rhythm. A circadian rhythm is a biological process in an organism that recurs with a regularity of approximately 24 hours.
regulation of DNA-templated transcription Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription.
regulation of transcription by RNA polymerase II Any process that modulates the frequency, rate or extent of transcription mediated by RNA polymerase II.
sperm DNA condensation The progressive compaction of the spermatid chromatin so that it reaches a level of condensation that is not compatible with nuclear activities such as transcription or DNA replication.
sperm DNA decondensation Unwinding of the condensed nuclear chromatin of an inactive male pronucleus after fertilization.
spermatogenesis The developmental process by which male germ line stem cells self renew or give rise to successive cell types resulting in the development of a spermatozoa.

17 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P38144 ISW1 ISWI chromatin-remodeling complex ATPase ISW1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
Q3B7N1 CHD1L Chromodomain-helicase-DNA-binding protein 1-like Bos taurus (Bovine) SS
Q9VL72 Etl1 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1 homolog Drosophila melanogaster (Fruit fly) PR
Q86WJ1 CHD1L Chromodomain-helicase-DNA-binding protein 1-like Homo sapiens (Human) EV
Q9NRZ9 HELLS Lymphoid-specific helicase Homo sapiens (Human) PR
O60264 SMARCA5 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 Homo sapiens (Human) EV
P28370 SMARCA1 Probable global transcription activator SNF2L1 Homo sapiens (Human) SS
Q60848 Hells Lymphocyte-specific helicase Mus musculus (Mouse) PR
Q9CXF7 Chd1l Chromodomain-helicase-DNA-binding protein 1-like Mus musculus (Mouse) SS
P40201 Chd1 Chromodomain-helicase-DNA-binding protein 1 Mus musculus (Mouse) PR
Q91ZW3 Smarca5 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 Mus musculus (Mouse) SS
Q6PGB8 Smarca1 Probable global transcription activator SNF2L1 Mus musculus (Mouse) SS
Q7G8Y3 Os01g0367900 Probable chromatin-remodeling complex ATPase chain Oryza sativa subsp japonica (Rice) PR
P41877 isw-1 Chromatin-remodeling complex ATPase chain isw-1 Caenorhabditis elegans SS
Q22516 chd-3 Chromodomain-helicase-DNA-binding protein 3 homolog Caenorhabditis elegans PR
Q9XFH4 DDM1 ATP-dependent DNA helicase DDM1 Arabidopsis thaliana (Mouse-ear cress) PR
Q8RWY3 CHR11 ISWI chromatin-remodeling complex ATPase CHR11 Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MSKTDTAAVE ATEENSNETT SDAATSSSGE KEAEFDNKIE ADRSRRFDFL LKQTEIFTHF
70 80 90 100 110 120
MTNSAKSPTK PKGRPKKIKD KDKEKDVADH RHRKTEQEED EELLAEDSAT KEIFRFDASP
130 140 150 160 170 180
AYIKSGEMRD YQIRGLNWMI SLYENGINGI LADEMGLGKT LQTISLLGYL KHFKNQAGPH
190 200 210 220 230 240
IVIVPKSTLQ NWVNEFKKWC PSLRAVCLIG DQDTRNTFIR DVLMPGEWDV CVTSYEMCIR
250 260 270 280 290 300
EKSVFKKFNW RYLVIDEAHR IKNEKSKLSE ILREFKTANR LLITGTPLQN NLHELWALLN
310 320 330 340 350 360
FLLPDVFNSS EDFDEWFNTN TCLGDDALIT RLHAVLKPFL LRRLKAEVEK RLKPKKEMKI
370 380 390 400 410 420
FVGLSKMQRD WYTKVLLKDI DVVNGAGKVE KMRLQNILMQ LRKCTNHPYL FDGAEPGPPY
430 440 450 460 470 480
TTDTHLVYNS GKMAILDKLL PKLQEQGSRV LIFSQMTRML DILEDYCHWR NYNYCRLDGQ
490 500 510 520 530 540
TPHEDRNRQI QEFNMDNSAK FLFMLSTRAG GLGINLATAD VVIIYDSDWN PQMDLQAMDR
550 560 570 580 590 600
AHRIGQKKQV RVFRLITEST VEEKIVERAE VKLRLDKMVI QGGRLVDNRS NQLNKDEMLN
610 620 630 640 650 660
IIRFGANQVF SSKETDITDE DIDVILERGE AKTAEQKAAL DSLGESSLRT FTMDTNGEAG
670 680 690 700 710 720
TSSVYQFEGE DWREKQKLNA LGNWIEPPKR ERKANYAVDA YFREALRVSE PKAPKAPRPP
730 740 750 760 770 780
KQPIVQDFQF FPPRLFELLD QEIYYFRKTV GYKVPKNTEL GSDATKVQRE EQRKIDEAEP
790 800 810 820 830 840
LTEEEIQEKE NLLSQGFTAW TKRDFNQFIK ANEKYGRDDI DNIAKDVEGK TPEEVIEYNA
850 860 870 880 890 900
VFWERCTELQ DIERIMGQIE RGEGKIQRRL SIKKALDQKM SRYRAPFHQL RLQYGNNKGK
910 920 930 940 950 960
NYTEIEDRFL VCMLHKLGFD KENVYEELRA AIRASPQFRF DWFIKSRTAL ELQRRCNTLI
970 980 990 1000 1010 1020
TLIERENIEL EEKERAEKKK KAPKGSVSAG SGSASSNTPA PAPQPKASQK RKSEVVATSS
NSKKKKK