Q19969
SS
Gene name |
ima-3 (F32E10.4) |
Protein name |
Importin subunit alpha-3 |
Names |
Karyopherin subunit alpha-3 |
Species |
Caenorhabditis elegans |
KEGG Pathway |
cel:CELE_F32E10.4 |
EC number |
|
Protein Class |
|
Descriptions
Importin-α is a nuclear import receptor that facilitates the transport of proteins with nuclear localization sequences (NLSs) into the nucleus. It is autoinhibited by its N-terminal region, which occupies the NLS-binding site, preventing unnecessary binding and import activity. Importin-α is autoinhibited in the absence of importin-β and the binding of importin-β alleviates this autoinhibition, which displaces the autoinhibitory sequence.
Autoinhibitory domains (AIDs)
Target domain |
130-222 (Major NLS-binding site);299-387 (Minor NLS-binding site) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
No accessory elements
References
- Fontes MR et al. (2000) "Structural basis of recognition of monopartite and bipartite nuclear localization sequences by mammalian importin-alpha", Journal of molecular biology, 297, 1183-94
- Fontes MR et al. (2003) "Structural basis for the specificity of bipartite nuclear localization sequence binding by importin-alpha", The Journal of biological chemistry, 278, 27981-7
- Catimel B et al. (2001) "Biophysical characterization of interactions involving importin-alpha during nuclear import", The Journal of biological chemistry, 276, 34189-98
- Pumroy RA et al. (2015) "Molecular determinants for nuclear import of influenza A PB2 by importin α isoforms 3 and 7", Structure (London, England : 1993), 23, 374-84
Autoinhibited structure
Activated structure
1 structures for Q19969
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q19969-F1 | Predicted | AlphaFoldDB |
No variants for Q19969
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for Q19969 | |||||
No associated diseases with Q19969
1 regional properties for Q19969
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Basic-leucine zipper domain | 272 - 337 | IPR004827 |
5 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| nuclear envelope | The double lipid bilayer enclosing the nucleus and separating its contents from the rest of the cytoplasm; includes the intermembrane space, a gap of width 20-40 nm (also called the perinuclear space). |
| nuclear pore | A protein complex providing a discrete opening in the nuclear envelope of a eukaryotic cell, where the inner and outer nuclear membranes are joined. |
| nucleoplasm | That part of the nuclear content other than the chromosomes or the nucleolus. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
3 GO annotations of molecular function
| Name | Definition |
|---|---|
| DNA-binding transcription factor binding | Binding to a DNA-binding transcription factor, a protein that interacts with a specific DNA sequence (sometimes referred to as a motif) within the regulatory region of a gene to modulate transcription. |
| nuclear import signal receptor activity | Combining with a nuclear import signal (NIS) on a cargo to be transported, to mediate transport of the cargo through the nuclear pore, from the cytoplasm to the nuclear lumen. The cargo can be either a RNA or a protein. |
| nuclear localization sequence binding | Binding to a nuclear localization sequence, a specific peptide sequence that acts as a signal to localize the protein within the nucleus. |
11 GO annotations of biological process
| Name | Definition |
|---|---|
| embryo development ending in birth or egg hatching | The process whose specific outcome is the progression of an embryo over time, from zygote formation until the end of the embryonic life stage. The end of the embryonic life stage is organism-specific and may be somewhat arbitrary; for mammals it is usually considered to be birth, for insects the hatching of the first instar larva from the eggshell. |
| female gamete generation | Generation of the female gamete; specialised haploid cells produced by meiosis and along with a male gamete takes part in sexual reproduction. |
| gamete generation | The generation and maintenance of gametes in a multicellular organism. A gamete is a haploid reproductive cell. |
| meiosis I | The first meiotic nuclear division in which homologous chromosomes are paired and segregated from each other, producing two haploid daughter nuclei. |
| nematode larval development | The process whose specific outcome is the progression of the nematode larva over time, from its formation to the mature structure. Nematode larval development begins with the newly hatched first-stage larva (L1) and ends with the end of the last larval stage (for example the fourth larval stage (L4) in C. elegans). Each stage of nematode larval development is characterized by proliferation of specific cell lineages and an increase in body size without alteration of the basic body plan. Nematode larval stages are separated by molts in which each stage-specific exoskeleton, or cuticle, is shed and replaced anew. |
| NLS-bearing protein import into nucleus | The directed movement of a protein bearing a nuclear localization signal (NLS) from the cytoplasm into the nucleus, across the nuclear envelope. |
| nuclear pore organization | A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the nuclear pore. |
| oogenesis | The complete process of formation and maturation of an ovum or female gamete from a primordial female germ cell. Examples of this process are found in Mus musculus and Drosophila melanogaster. |
| positive regulation of axon extension involved in regeneration | Any process that activates, maintains or increases the rate of axon extension involved in regeneration. |
| protein import into nucleus | The directed movement of a protein from the cytoplasm to the nucleus. |
| reproduction | The production of new individuals that contain some portion of genetic material inherited from one or more parent organisms. |
30 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q02821 | SRP1 | Importin subunit alpha | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | SS |
| Q0V7M0 | KPNA6 | Importin subunit alpha-7 | Bos taurus (Bovine) | SS |
| A2VE08 | KPNA1 | Importin subunit alpha-5 | Bos taurus (Bovine) | SS |
| C1JZ66 | KPNA7 | Importin subunit alpha-8 | Bos taurus (Bovine) | PR |
| Q5ZML1 | KPNA1 | Importin subunit alpha-5 | Gallus gallus (Chicken) | SS |
| P52295 | Pen | Importin subunit alpha | Drosophila melanogaster (Fruit fly) | SS |
| P52294 | KPNA1 | Importin subunit alpha-5 | Homo sapiens (Human) | SS |
| O15131 | KPNA5 | Importin subunit alpha-6 | Homo sapiens (Human) | SS |
| O60684 | KPNA6 | Importin subunit alpha-7 | Homo sapiens (Human) | SS |
| A9QM74 | KPNA7 | Importin subunit alpha-8 | Homo sapiens (Human) | EV |
| P52292 | KPNA2 | Importin subunit alpha-1 | Homo sapiens (Human) | EV |
| O00505 | KPNA3 | Importin subunit alpha-4 | Homo sapiens (Human) | SS |
| O00629 | KPNA4 | Importin subunit alpha-3 | Homo sapiens (Human) | SS |
| Q60960 | Kpna1 | Importin subunit alpha-5 | Mus musculus (Mouse) | SS |
| O35345 | Kpna6 | Importin subunit alpha-7 | Mus musculus (Mouse) | PR |
| C0LLJ0 | Kpna7 | Importin subunit alpha-8 | Mus musculus (Mouse) | SS |
| P52293 | Kpna2 | Importin subunit alpha-1 | Mus musculus (Mouse) | EV |
| O35343 | Kpna4 | Importin subunit alpha-3 | Mus musculus (Mouse) | SS |
| O35344 | Kpna3 | Importin subunit alpha-4 | Mus musculus (Mouse) | SS |
| C6K7I2 | KPNA7 | Importin subunit alpha-8 | Sus scrofa (Pig) | SS |
| P83953 | Kpna1 | Importin subunit alpha-5 | Rattus norvegicus (Rat) | SS |
| Q56R16 | Kpna5 | Importin subunit alpha-6 | Rattus norvegicus (Rat) | SS |
| Q71VM4 | Os01g0253300 | Importin subunit alpha-1a | Oryza sativa subsp. japonica (Rice) | SS |
| P91276 | ima-2 | Importin subunit alpha-2 | Caenorhabditis elegans | SS |
| O04294 | IMPA3 | Importin subunit alpha-3 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9FJ09 | IMPA5 | Importin subunit alpha-5 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9M9X7 | IMPA7 | Importin subunit alpha-7 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9FWY7 | IMPA6 | Importin subunit alpha-6 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| O22478 | Importin subunit alpha | Solanum lycopersicum (Tomato) (Lycopersicon esculentum) | SS | |
| Q503E9 | kpna5 | Importin subunit alpha-6 | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSSNRQAYYK | NNAKEQIGKE | KRNEEVVSIR | KDKREEAISK | RRNINTQIED | DSETSTTPPG |
| 70 | 80 | 90 | 100 | 110 | 120 |
| PFDANLLRLT | VAAAQSSDPA | EQLTAVQQAR | KMLSTDRNPP | IDDLIGSGIL | PVLVQCLSST |
| 130 | 140 | 150 | 160 | 170 | 180 |
| DPNLQFEAAW | ALTNIASGTS | EQTQAVVNAG | AVPLFLQLLS | CGNLNVCEQS | VWALGNIIGD |
| 190 | 200 | 210 | 220 | 230 | 240 |
| GPHFRDYCLE | LGILQPLLQF | INPEIPIGFL | RNVTWVIVNL | CRCKDPAPSP | AVVRTILPAL |
| 250 | 260 | 270 | 280 | 290 | 300 |
| SLLIHHQDTN | ILIDTVWALS | YLTDGGNEHI | QMVIEAQVVT | HLVPLLGHVD | VKVQTAALRA |
| 310 | 320 | 330 | 340 | 350 | 360 |
| VGNIVTGTDE | QTQLVLDSGV | LRFMPGLLAH | YKEKINKEAV | WFVSNITAGN | QQQVQDVFDA |
| 370 | 380 | 390 | 400 | 410 | 420 |
| GIMPMIIHLL | DRGDFPTQKE | AAWAISNVTI | SGRPNQVEQM | VKLGVLRPFC | AMLSCTDSQI |
| 430 | 440 | 450 | 460 | 470 | 480 |
| IQVVLDGINN | ILKMAGEAAE | QVTSEIEECG | GLDKIENLQN | HENEDIYKLA | FEIIDNFFSS |
| 490 | 500 | 510 | |||
| DDETGNVEGA | QSSAFGGDVP | PVPDAPNGGW | NFGK |