Q19266

SS
Gene name
pkc-3 (aPKC, F09E5.1)
Protein name
Protein kinase C-like 3
Names
EC 2.7.11.13 , Atypical protein kinase C-3 , aPKC3
Species
Caenorhabditis elegans
KEGG Pathway
cel:CELE_F09E5.1
EC number
2.7.11.13: Protein-serine/threonine kinases
Protein Class

Descriptions

PRKCI, an atypical PKC (aPKC) isoform, is an important kinase in multiple cellular processes and regulated by the phosphatidylinositol 3-kinase product phosphatidylinositol 3,4,5-(PO4)3 (PIP3). In aPKC molecule, the arginine motif (123-140) in the pseudosubstrate region on the regulatory domain binds to acidic residues in the substrate-binding region on the catalytic domain, leading to the off state of aPKCs. The autoinhibition of aPKC is released by binding with the acidic ligand PIP3 and consequently exposing the substrate-binding site.

Autoinhibitory domains (AIDs)

110-127 (Pseudosubstrate region) SS

Target domain

230-597 (Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C iota)

Relief mechanism

Ligand binding

Assay

Accessory elements

394-417 (Activation loop from InterPro)

Target domain

257-586 (Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C)

Relief mechanism

Assay

394-417 (Activation loop from InterPro)

Target domain

253-586 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for Q19266

Entry ID Method Resolution Chain Position Source
AF-Q19266-F1 Predicted AlphaFoldDB

No variants for Q19266

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q19266

No associated diseases with Q19266

2 regional properties for Q19266

Type Name Position InterPro Accession
domain Nuclear hormone receptor, ligand-binding domain 669 - 903 IPR000536
domain Zinc finger, nuclear hormone receptor-type 554 - 629 IPR001628

Functions

Description
EC Number 2.7.11.13 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Cytoplasm, cytoskeleton
  • Targeted and anchored at the apical surface (villi) of intestinal and pharyngeal cells, and in proximity with the cortical actin cytoskeleton that lies under the plasma membrane
  • Tightly bound to organelles/cytoskeleton in six of the seven developmental stages
  • Accumulation in cytoplasm is restricted to L1 larvae and adults
  • Colocalized with par-3 at the anterior cortex of the 1-cell embryo
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

7 GO annotations of cellular component

Name Definition
apical plasma membrane The region of the plasma membrane located at the apical end of the cell.
cell cortex The region of a cell that lies just beneath the plasma membrane and often, but not always, contains a network of actin filaments and associated proteins.
cortical actin cytoskeleton The portion of the actin cytoskeleton, comprising filamentous actin and associated proteins, that lies just beneath the plasma membrane.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
membrane A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

7 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
diacylglycerol-dependent serine/threonine kinase activity Catalysis of the reaction
metal ion binding Binding to a metal ion.
protein domain specific binding Binding to a specific domain of a protein.
protein kinase activity Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions

12 GO annotations of biological process

Name Definition
cell differentiation The cellular developmental process in which a relatively unspecialized cell, e.g. embryonic or regenerative cell, acquires specialized structural and/or functional features that characterize a specific cell. Differentiation includes the processes involved in commitment of a cell to a specific fate and its subsequent development to the mature state.
defense response to fungus Reactions triggered in response to the presence of a fungus that act to protect the cell or organism.
embryo development ending in birth or egg hatching The process whose specific outcome is the progression of an embryo over time, from zygote formation until the end of the embryonic life stage. The end of the embryonic life stage is organism-specific and may be somewhat arbitrary; for mammals it is usually considered to be birth, for insects the hatching of the first instar larva from the eggshell.
establishment or maintenance of cell polarity Any cellular process that results in the specification, formation or maintenance of anisotropic intracellular organization or cell growth patterns.
gastrulation A complex and coordinated series of cellular movements that occurs at the end of cleavage during embryonic development of most animals. The details of gastrulation vary from species to species, but usually result in the formation of the three primary germ layers, ectoderm, mesoderm and endoderm.
gonad development The process whose specific outcome is the progression of the gonad over time, from its formation to the mature structure. The gonad is an animal organ that produces gametes; in some species it also produces hormones.
gonadal mesoderm development The process whose specific outcome is the progression of the gonadal mesoderm over time, from its formation to the mature structure. The gonadal mesoderm is the middle layer of the three primary germ layers of the embryo which will go on to form the gonads of the organism.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
protein localization Any process in which a protein is transported to, or maintained in, a specific location.
response to wounding Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating damage to the organism.
single fertilization The union of male and female gametes to form a zygote.

15 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P24583 PKC1 Protein kinase C-like 1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SS
A1Z9X0 aPKC Atypical protein kinase C Drosophila melanogaster (Fruit fly) SS
P41743 PRKCI Protein kinase C iota type Homo sapiens (Human) EV
Q05513 PRKCZ Protein kinase C zeta type Homo sapiens (Human) SS
Q62074 Prkci Protein kinase C iota type Mus musculus (Mouse) SS
Q02956 Prkcz Protein kinase C zeta type Mus musculus (Mouse) SS
F1M7Y5 Prkci Protein kinase C iota type Rattus norvegicus (Rat) SS
P09217 Prkcz Protein kinase C zeta type Rattus norvegicus (Rat) SS
Q17941 akt-1 Serine/threonine-protein kinase akt-1 Caenorhabditis elegans PR
Q9XTG7 akt-2 Serine/threonine-protein kinase akt-2 Caenorhabditis elegans SS
Q9Y1J3 pdk-1 3-phosphoinositide-dependent protein kinase 1 Caenorhabditis elegans SS
P34722 tpa-1 Protein kinase C-like 1 Caenorhabditis elegans PR
P90980 pkc-2 Protein kinase C-like 2 Caenorhabditis elegans SS
Q2L6W9 sax-1 Serine/threonine-protein kinase sax-1 Caenorhabditis elegans SS
Q90XF2 prkci Protein kinase C iota type Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MSSPTSLEED GDIKLKTRFQ GQVVVLYARP PLILDDFFAL LKDACKQHKK QDITVKWIDE
70 80 90 100 110 120
DGDPISIDSQ MELDEAVRCL NSSQEAELNI HVFVGKPELP GLPCQGEDKT VYRRGARRWK
130 140 150 160 170 180
KIYLYNGHRF QAKRLNRRIQ CFICHDYIWG IGRQGFRCVD CRLCVHKKCH RHVRTHCGQA
190 200 210 220 230 240
LQGPNIIPMA PASGSLKGAR SNTSSSTTRS GGGIDNGAFH EHEIESPGST SHDASRAMNG
250 260 270 280 290 300
NGSSKWAVSL NDFRLLTVIG RGSYAKVVQA EHVSTRQIYA IKIIKKEMFN EDEDIDWVQT
310 320 330 340 350 360
EKSVFEAASN YPFLVGLHSC FQTESRLFFV IEFVPGGDLM FHMQQQRKLP EEHARFYSGE
370 380 390 400 410 420
IILALHFLHS RGIIYRDLKL DNVLIDAEGH IKLTDYGMCK ENIKDGDLTS TFCGTPNYIA
430 440 450 460 470 480
PEILRGDEYG FSVDWWALGV LMFEMMAGRS PFDIVGMQNS EENTEDYLFQ IILERQIRIP
490 500 510 520 530 540
RSLSVRASGI LKGFLNKDPT ERLGCKLDIN EGLRDMKEHQ FFRGFIDWEA LEQKAVAPPY
550 560 570 580 590
HPAVESDRDL THFDHQFTDE LPQLSPDNPD VIARIDQSEF DGFEYVNPLQ MSREDSV