AiPD: Autoinhibited Protein Database

Q15691

Gene name	MAPRE1
Protein name	Microtubule-associated protein RP/EB family member 1
Names	APC-binding protein EB1, End-binding protein 1, EB1
Species	Homo sapiens (Human)
KEGG Pathway	hsa:22919
EC number
Protein Class	MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER (PTHR10623)

Descriptions

EB1 including a Calponin homology (CH) domain and the C-terminal dimerization domain is a plus-end tracking protein that binds to the plus-end of microtubules (MT) and regulates the dynamics of the microtubule cytoskeleton. The CH domain is responsible for MT-binding and inhibited by the binding of the CH domain and the central region of the C-terminal domain including the FYF motif. SxIP competes with the CH domain and binds on the FYF motif of the C-terminal domain, and activates the CH domain.

Autoinhibitory domains (AIDs)

Target domain	1-130 (CH domain)
Relief mechanism	Partner binding
Assay	Structural analysis

AID

249-268 (EB1 autoinhibitory tail)

Target domain

1-130 (CH domain)

Relief mechanism

Partner binding (SxlP binding)

Assay

Structural analysis

Accessory elements

No accessory elements

References

Kanaba T et al. (2013) "Microtubule-binding sites of the CH domain of EB1 and its autoinhibition revealed by NMR", Biochimica et biophysica acta, 1834, 499-507

Autoinhibited structure

Activated structure

32 structures for Q15691

Entry ID	Method	Resolution	Chain	Position	Source
1PA7	X-ray	145 A	A	1-130	PDB
1TXQ	X-ray	180 A	B	183-268	PDB
1UEG	X-ray	240 A	A	1-130	PDB
1VKA	X-ray	160 A	A/B	2-140	PDB
1WU9	X-ray	154 A	A/B	191-268	PDB
1YIB	X-ray	180 A	A	185-255	PDB
1YIG	X-ray	200 A	A/B	185-255	PDB
2HKQ	X-ray	186 A	A	191-268	PDB
2HL3	X-ray	203 A	C	263-268	PDB
2HL5	X-ray	193 A	A/B	191-268	PDB
2QJZ	X-ray	125 A	A/B	12-133	PDB
2R8U	X-ray	135 A	A/B	1-268	PDB
3GJO	X-ray	250 A	A/B/C/D	191-260	PDB
3MTU	X-ray	210 A	A/B/C/D	215-257	PDB
3MUD	X-ray	220 A	C/D	226-257	PDB
3TQ7	X-ray	230 A	A	191-268	PDB
4XA1	X-ray	320 A	A/B/C/D	211-251	PDB
4XA3	X-ray	255 A	A/B	215-251	PDB
4XA6	X-ray	342 A	A/B/C/D	209-251	PDB
5JV3	X-ray	201 A	A/B/C/D	210-257	PDB
5JVM	X-ray	157 A	A/B	207-257	PDB
5JVP	X-ray	210 A	A/B/C/D/E/F	210-257	PDB
5JVR	X-ray	210 A	A/B/C/D/E/F/G/H	207-257	PDB
5JVS	X-ray	225 A	A	207-257	PDB
5JVU	X-ray	195 A	A/B	207-257	PDB
5JX1	X-ray	167 A	A	210-257	PDB
5WLQ	X-ray	310 A	A	208-257	PDB
6PF2	X-ray	217 A	A/B	207-257	PDB
6PFP	X-ray	220 A	A/B/C/D	207-257	PDB
6YF5	X-ray	183 A	A/B/C/D	215-251	PDB
6YSH	X-ray	283 A			PDB
AF-Q15691-F1	Predicted				AlphaFoldDB

142 variants for Q15691

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
CA9810950 rs747431102	2	A>V		No	ClinGen ExAC gnomAD
COSM1025815 CA9810952 rs781480234	8	T>M	endometrium [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed gnomAD
CA408596437 rs1409080565	18	H>R		No	ClinGen TOPMed gnomAD
CA9810954 rs770214521	20	M>L		No	ClinGen ExAC gnomAD
CA9810959 COSM1751387 rs149760630	25	N>S	urinary_tract [Cosmic]	No	ClinGen cosmic curated ESP ExAC TOPMed gnomAD
rs1328471287 CA408596510	29	Q>K		No	ClinGen gnomAD
rs761590869 CA9810960	29	Q>R		No	ClinGen ExAC TOPMed gnomAD
CA9810962 rs750450777	31	N>H		No	ClinGen ExAC gnomAD
rs760515512 CA9810963	33	T>P		No	ClinGen ExAC gnomAD
rs1229840672 CA408596553	35	I>T		No	ClinGen gnomAD
CA9810965 rs753378763	36	E>K		No	ClinGen ExAC gnomAD
rs759050147 CA9810985	43	A>V		No	ClinGen ExAC TOPMed gnomAD
CA313184493 rs750952621	48	M>T		No	ClinGen Ensembl
rs764931834 CA9810986	50	M>L		No	ClinGen ExAC gnomAD
CA9810987 rs752362583	55	S>C		No	ClinGen ExAC gnomAD
rs193132345 CA9810989	56	I>T		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA9810988 rs145784113	56	I>V		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs1412524528 CA408598471	57	A>V		No	ClinGen gnomAD
CA408598574 rs1340476676	63	F>S		No	ClinGen gnomAD
CA9810991 rs756646311	64	Q>R		No	ClinGen ExAC gnomAD
rs79232877 CA313184536	68	E>A		No	ClinGen Ensembl
rs372631624 CA9810994	73	Q>H		No	ClinGen ESP ExAC TOPMed gnomAD
CA313184571 rs867063168	74	N>K		No	ClinGen Ensembl
CA408598854 rs1382471603	78	L>I		No	ClinGen TOPMed
rs772229436 CA9810997	81	G>A		No	ClinGen ExAC TOPMed gnomAD
COSM1025818 CA9811000 rs770912318	87	V>A	endometrium [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
CA9810999 rs138467047	87	V>I		No	ClinGen 1000Genomes ExAC gnomAD
CA9811030 rs761353179	90	I>V		No	ClinGen ExAC gnomAD
CA313186916 rs1044144737	95	K>R		No	ClinGen gnomAD
rs1329886748 CA408600091	100	K>R		No	ClinGen gnomAD
rs1448823795 CA408600129	103	D>N		No	ClinGen gnomAD
CA408600188 rs1369523909	107	F>I		No	ClinGen TOPMed
CA313186945 rs371042900	108	V>I		No	ClinGen ESP TOPMed
rs759988702 CA9811033	110	W>R		No	ClinGen ExAC gnomAD
rs375430356 CA9811034	112	K>N		No	ClinGen ESP ExAC TOPMed gnomAD
CA408600265 rs1192205197	112	K>R		No	ClinGen gnomAD
rs1468161617 CA408600280	113	K>R		No	ClinGen TOPMed
CA313187001 rs11542529	115	F>L		No	ClinGen Ensembl
CA9811036 rs758889318	116	D>N		No	ClinGen ExAC TOPMed
rs142135206 CA9811037	119	Y>C		No	ClinGen ESP ExAC TOPMed gnomAD
rs1804572 CA313187070	122	K>Q		No	ClinGen Ensembl
rs757339672 CA9811039	123	D>G		No	ClinGen ExAC gnomAD
CA408600423 rs1348118681	124	Y>C		No	ClinGen gnomAD
rs892043710 CA408600442	125	D>E		No	ClinGen gnomAD
CA9811040 rs781154555	125	D>N		No	ClinGen ExAC gnomAD
rs1318689380 CA408600445	126	P>A		No	ClinGen TOPMed
rs1290393107 CA408600487	130	R>G		No	ClinGen gnomAD
CA313187117 rs902142607	130	R>S		No	ClinGen TOPMed gnomAD
CA9811042 rs769627024	130	R>T		No	ClinGen ExAC TOPMed gnomAD
rs1010864247 CA313187121	132	G>R		No	ClinGen Ensembl
rs1440977605 CA408600523	133	Q>K		No	ClinGen TOPMed
rs1383556034 CA408600576	137	V>L		No	ClinGen TOPMed
rs779770995 CA9811043	138	A>G		No	ClinGen ExAC TOPMed gnomAD
rs1253591952 CA408600617	140	S>F		No	ClinGen gnomAD
CA408600610 rs1202620597	140	S>P		No	ClinGen TOPMed gnomAD
rs189725550 CA9811046	141	L>F		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs1601167208 CA408600630	141	L>P		No	ClinGen Ensembl
rs189725550 CA408600620	141	L>V		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs1159620955 CA408600646	142	V>A		No	ClinGen TOPMed
rs761390778 CA9811047	142	V>F		No	ClinGen ExAC TOPMed gnomAD
CA408600660 rs1454157355	143	A>V		No	ClinGen TOPMed
CA313187144 rs146207631	144	P>A		No	ClinGen ESP TOPMed gnomAD
rs771566682 CA9811048	145	A>V		No	ClinGen ExAC TOPMed gnomAD
CA408600693 rs1372310592	146	L>V		No	ClinGen gnomAD
CA408600724 rs1434002736	147	N>S		No	ClinGen TOPMed
rs760030558 CA9811050	149	P>L		No	ClinGen ExAC gnomAD
CA9811049 rs772601473	149	P>S		No	ClinGen ExAC gnomAD
rs763380881 CA9811053	151	K>R		No	ClinGen ExAC gnomAD
rs1486773300 CA408600868	152	P>H		No	ClinGen TOPMed
rs1295435956 CA408600880	153	L>F		No	ClinGen gnomAD
rs889504929 CA313187212	154	T>I		No	ClinGen TOPMed
rs764603778 CA9811054	154	T>P		No	ClinGen ExAC gnomAD
CA408600920 rs1384967822	156	S>G		No	ClinGen gnomAD
CA408600958 rs1568880625	157	S>G		No	ClinGen Ensembl
rs1209850781 CA408600964	157	S>N		No	ClinGen TOPMed
CA408601913 rs1307890379	159	A>V		No	ClinGen TOPMed
CA408601921 rs1261861903	160	P>L		No	ClinGen TOPMed gnomAD
rs753732974 CA9811085	162	R>K		No	ClinGen ExAC TOPMed gnomAD
CA9811086 rs754910273	163	P>T		No	ClinGen ExAC gnomAD
CA408601946 rs1276968138	164	I>M		No	ClinGen gnomAD
CA9811087 rs778621807	164	I>V		No	ClinGen ExAC gnomAD
rs747882078 CA9811088	166	T>A		No	ClinGen ExAC TOPMed gnomAD
rs531184486 CA9811089	167	Q>H		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs1349597284 CA408601976	169	T>S		No	ClinGen TOPMed
CA9811091 rs746726671	170	A>T		No	ClinGen ExAC TOPMed gnomAD
CA9811093 rs199851862	171	A>E		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA9811095 rs199851862	171	A>G		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs374092179 CA313189669	171	A>P		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs374092179 CA9811092	171	A>T		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA9811094 rs199851862	171	A>V		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA9811097 rs371732896	172	A>S		No	ClinGen ESP ExAC gnomAD
CA408602004 rs1176789708	175	A>P		No	ClinGen TOPMed gnomAD
CA408602003 rs1176789708	175	A>T		No	ClinGen TOPMed gnomAD
rs781433148 CA9811098	176	G>V		No	ClinGen ExAC gnomAD
rs773256853 CA9811099	178	G>D		No	ClinGen ExAC gnomAD
CA408602024 rs773256853	178	G>V		No	ClinGen ExAC gnomAD
rs372041005 CA9811100	179	V>M		No	ClinGen ESP ExAC gnomAD
rs1601169155 CA408602034	180	V>G		No	ClinGen Ensembl
CA313189720 rs547725771	181	R>Q		No	ClinGen 1000Genomes TOPMed
COSM1025819 CA9811102 rs753923516	182	K>N	endometrium [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
rs1436792065 CA408602059	184	P>L		No	ClinGen gnomAD
CA9811103 rs755109122	184	P>S		No	ClinGen ExAC gnomAD
CA408602066 rs1490402189	186	V>M		No	ClinGen TOPMed
CA408602075 rs1284931665	187	G>D		No	ClinGen TOPMed
CA9811105 rs376629657	188	N>S		No	ClinGen ESP ExAC TOPMed gnomAD
rs964590656 CA313189753	189	G>R		No	ClinGen gnomAD
rs374382367 CA408602111	191	D>E		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA408602100 rs1226468547	191	D>N		No	ClinGen gnomAD
CA9811108 rs746622138	192	E>K		No	ClinGen ExAC gnomAD
rs756974223 CA9811109	194	A>P		No	ClinGen ExAC TOPMed gnomAD
rs769499986 CA9811112	197	M>I		No	ClinGen ExAC gnomAD
rs137996927 CA9811111	197	M>T		No	ClinGen ESP ExAC gnomAD
rs1472099855 CA408602192	198	Q>H		No	ClinGen gnomAD
CA313189806 rs866284040	198	Q>R		No	ClinGen gnomAD
CA408603127 rs1295136563	200	V>G		No	ClinGen gnomAD
rs41289870 CA9811135	201	N>S		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs771324465 CA9811137	202	V>I		No	ClinGen ExAC TOPMed gnomAD
rs771324465 CA408603143	202	V>L		No	ClinGen ExAC TOPMed gnomAD
CA9811138 rs776809915	206	T>A		No	ClinGen ExAC gnomAD
CA9811139 rs759774837	206	T>N		No	ClinGen ExAC gnomAD
rs200062626 CA313195224	207	V>I		No	ClinGen 1000Genomes gnomAD
rs1056327244 CA313195234	208	E>D		No	ClinGen Ensembl
CA408603264 rs1450531600	209	D>G		No	ClinGen gnomAD
rs775645885 CA9811141	209	D>H		No	ClinGen ExAC gnomAD
rs1376780451 CA408603316	214	R>K		No	ClinGen TOPMed gnomAD
rs751616832 CA9811144	222	R>W		No	ClinGen ExAC gnomAD
rs928584069 CA313195270	227	I>V		No	ClinGen TOPMed
CA9811148 rs755792170	232	E>D		No	ClinGen ExAC gnomAD
CA9811147 rs371221771	232	E>K		No	ClinGen ESP ExAC gnomAD
CA9811149 rs765746857	233	G>R		No	ClinGen ExAC TOPMed gnomAD
CA9811151 rs754509909	236	D>N		No	ClinGen ExAC TOPMed gnomAD
rs778221336 CA9811152	238	V>I		No	ClinGen ExAC TOPMed gnomAD
CA9811154 rs757567678	245	I>T		No	ClinGen ExAC gnomAD
rs781569841 CA9811155	250	D>Y		No	ClinGen ExAC gnomAD
rs756503037 CA9811175	254	V>M		No	ClinGen ExAC gnomAD
rs749526942 CA9811177	256	P>L		No	ClinGen ExAC gnomAD
rs780296338 CA9811176	256	P>S		No	ClinGen ExAC gnomAD
CA408604282 rs1185691326	257	D>G		No	ClinGen TOPMed
rs768809491 CA9811180	260	G>A		No	ClinGen ExAC TOPMed gnomAD
rs768809491 CA9811179	260	G>D		No	ClinGen ExAC TOPMed gnomAD
CA9811178 rs768809491	260	G>V		No	ClinGen ExAC TOPMed gnomAD
CA9811182 rs201390649	264	E>A		No	ClinGen ESP ExAC TOPMed gnomAD

No associated diseases with Q15691

2 regional properties for Q15691

Type	Name	Position	InterPro Accession
domain	Calponin homology domain	14 - 117	IPR001715
domain	EB1, C-terminal	185 - 255	IPR004953

Functions

		Description
EC Number
Subcellular Localization	Cytoplasm, cytoskeleton Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Golgi apparatus Cytoplasm, cytoskeleton, spindle Cytoplasm, cytoskeleton, spindle pole	Associated with the microtubule growing distal tips (PubMed:28814570) Recruitment to the Golgi apparatus requires the presence of PDE4DIP isoform 13/MMG8/SMYLE (PubMed:25217626)
PANTHER Family	PTHR10623	MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER
PANTHER Subfamily	PTHR10623:SF20	MICROTUBULE-ASSOCIATED PROTEIN RP_EB FAMILY MEMBER 1
PANTHER Protein Class	non-motor microtubule binding protein
PANTHER Pathway Category	No pathway information available

13 GO annotations of cellular component

Name	Definition
cell projection membrane	The portion of the plasma membrane surrounding a plasma membrane bounded cell surface projection.
centrosome	A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
ciliary basal body	A membrane-tethered, short cylindrical array of microtubules and associated proteins found at the base of a eukaryotic cilium (also called flagellum) that is similar in structure to a centriole and derives from it. The cilium basal body is the site of assembly and remodelling of the cilium and serves as a nucleation site for axoneme growth. As well as anchoring the cilium, it is thought to provide a selective gateway regulating the entry of ciliary proteins and vesicles by intraflagellar transport.
cortical microtubule cytoskeleton	The portion of the microtubule cytoskeleton that lies just beneath the plasma membrane.
cytoplasmic microtubule	Any microtubule in the cytoplasm of a cell.
cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
focal adhesion	A cell-substrate junction that anchors the cell to the extracellular matrix and that forms a point of termination of actin filaments. In insects focal adhesion has also been referred to as hemi-adherens junction (HAJ).
Golgi apparatus	A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways.
microtubule	Any of the long, generally straight, hollow tubes of internal diameter 12-15 nm and external diameter 24 nm found in a wide variety of eukaryotic cells; each consists (usually) of 13 protofilaments of polymeric tubulin, staggered in such a manner that the tubulin monomers are arranged in a helical pattern on the microtubular surface, and with the alpha/beta axes of the tubulin subunits parallel to the long axis of the tubule; exist in equilibrium with pool of tubulin monomers and can be rapidly assembled or disassembled in response to physiological stimuli; concerned with force generation, e.g. in the spindle.
microtubule organizing center	An intracellular structure that can catalyze gamma-tubulin-dependent microtubule nucleation and that can anchor microtubules by interacting with their minus ends, plus ends or sides.
microtubule plus-end	The growing (plus) end of a microtubule. In vitro, microtubules polymerize more quickly at the plus end than at the minus end. In vivo, microtubule growth occurs only at the plus end, and the plus end switches between periods of growth and shortening, a behavior known as dynamic instability.
mitotic spindle astral microtubule end	Any microtubule end that is part of a mitotic spindle astral microtubule.
spindle midzone	The area in the center of the spindle where the spindle microtubules from opposite poles overlap.

6 GO annotations of molecular function

Name	Definition
cadherin binding	Binding to cadherin, a type I membrane protein involved in cell adhesion.
identical protein binding	Binding to an identical protein or proteins.
microtubule plus-end binding	Binding to the plus end of a microtubule.
protein C-terminus binding	Binding to a protein C-terminus, the end of a peptide chain at which the 1-carboxyl function of a constituent amino acid is not attached in peptide linkage to another amino-acid residue.
protein kinase binding	Binding to a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate.
RNA binding	Binding to an RNA molecule or a portion thereof.

14 GO annotations of biological process

Name	Definition
cell division	The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells.
cell migration	The controlled self-propelled movement of a cell from one site to a destination guided by molecular cues. Cell migration is a central process in the development and maintenance of multicellular organisms.
microtubule bundle formation	A process that results in a parallel arrangement of microtubules.
microtubule polymerization	The addition of tubulin heterodimers to one or both ends of a microtubule.
negative regulation of microtubule polymerization	Any process that stops, prevents, or reduces the frequency, rate or extent of microtubule polymerization.
non-motile cilium assembly	The aggregation, arrangement and bonding together of a set of components to form a non-motile cilium.
positive regulation of microtubule plus-end binding	Any process that activates or increases the frequency, rate or extent of microtubule plus-end binding.
positive regulation of microtubule polymerization	Any process that activates or increases the frequency, rate or extent of microtubule polymerization.
protein localization	Any process in which a protein is transported to, or maintained in, a specific location.
protein localization to centrosome	A process in which a protein is transported to, or maintained at, the centrosome.
protein localization to microtubule	A process in which a protein is transported to, or maintained at, a microtubule.
protein localization to microtubule plus-end	A process in which a protein is transported to, or maintained in, a location at a microtubule plus-end.
regulation of microtubule polymerization or depolymerization	Any process that modulates the frequency, rate or extent of microtubule polymerization or depolymerization by the addition or removal of tubulin heterodimers from a microtubule.
spindle assembly	The aggregation, arrangement and bonding together of a set of components to form the spindle, the array of microtubules and associated molecules that serves to move duplicated chromosomes apart.

12 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q3ZBD9	MAPRE1	Microtubule-associated protein RP/EB family member 1	Bos taurus (Bovine)	SS
Q5ZLC7	MAPRE1	Microtubule-associated protein RP/EB family member 1	Gallus gallus (Chicken)	SS
Q15555	MAPRE2	Microtubule-associated protein RP/EB family member 2	Homo sapiens (Human)	PR
Q9UPY8	MAPRE3	Microtubule-associated protein RP/EB family member 3	Homo sapiens (Human)	SS
Q8R001	Mapre2	Microtubule-associated protein RP/EB family member 2	Mus musculus (Mouse)	PR
Q61166	Mapre1	Microtubule-associated protein RP/EB family member 1	Mus musculus (Mouse)	SS
Q6PER3	Mapre3	Microtubule-associated protein RP/EB family member 3	Mus musculus (Mouse)	SS
Q5XIT1	Mapre3	Microtubule-associated protein RP/EB family member 3	Rattus norvegicus (Rat)	SS
Q66HR2	Mapre1	Microtubule-associated protein RP/EB family member 1	Rattus norvegicus (Rat)	SS
Q7XJ60	EB1A	Microtubule-associated protein RP/EB family member 1A	Arabidopsis thaliana (Mouse-ear cress)	SS
Q9FJJ5	EB1B	Microtubule-associated protein RP/EB family member 1B	Arabidopsis thaliana (Mouse-ear cress)	EV
Q6P848	mapre1	Microtubule-associated protein RP/EB family member 1	Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)	SS

10	20	30	40	50	60
MAVNVYSTSV	TSDNLSRHDM	LAWINESLQL	NLTKIEQLCS	GAAYCQFMDM	LFPGSIALKK
70	80	90	100	110	120
VKFQAKLEHE	YIQNFKILQA	GFKRMGVDKI	IPVDKLVKGK	FQDNFEFVQW	FKKFFDANYD
130	140	150	160	170	180
GKDYDPVAAR	QGQETAVAPS	LVAPALNKPK	KPLTSSSAAP	QRPISTQRTA	AAPKAGPGVV
190	200	210	220	230	240
RKNPGVGNGD	DEAAELMQQV	NVLKLTVEDL	EKERDFYFGK	LRNIELICQE	NEGENDPVLQ
250	260
RIVDILYATD	EGFVIPDEGG	PQEEQEEY