Q10664
Gene name |
mek-2 (Y54E10BL.6) |
Protein name |
Dual specificity mitogen-activated protein kinase kinase mek-2 |
Names |
MAP kinase kinase mek-2 |
Species |
Caenorhabditis elegans |
KEGG Pathway |
cel:CELE_Y54E10BL.6 |
EC number |
2.7.12.2: Dual-specificity kinases (those acting on Ser/Thr and Tyr residues) |
Protein Class |
|
Descriptions
(Annotation based on sequence homology with Q02750)
MAP2K1 encodes for Dual specificity mitogen-activated protein kinase kinase 1, belongs to MAP2Ks family, plays an important role in MAP kinase signal transduction pathway. The α-helix 1 in the activation loop of MAP2K1 is shifted outwards from the active site because of the NRD. NRD of MAP2K1 prevents MAP2K1 changing to the active conformation. The autoinhibitory state of MAP2K1 is more stable as dimer in the absence of its own substrates or other interacting partners. The several interactions span from the N-terminal to the C-terminal to form dimer. Dimerization of MAP2K1 block access of the substrate binding site and the activation loop from macromolecules.
Autoinhibitory domains (AIDs)
Target domain |
|
Relief mechanism |
|
Assay |
cis-regPred |
Accessory elements
212-233 (Activation loop from InterPro)
Target domain |
73-360 (Protein kinase domain) |
Relief mechanism |
|
Assay |
|
Autoinhibited structure
Activated structure
1 structures for Q10664
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q10664-F1 | Predicted | AlphaFoldDB |
No variants for Q10664
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for Q10664 | |||||
No associated diseases with Q10664
9 regional properties for Q10664
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | SH3 domain | 225 - 293 | IPR001452 |
| domain | PDZ domain | 140 - 219 | IPR001478 |
| domain | L27 domain | 8 - 121 | IPR004172 |
| domain | Guanylate kinase-like domain | 350 - 537 | IPR008144 |
| domain | Guanylate kinase/L-type calcium channel beta subunit | 349 - 540 | IPR008145 |
| domain | L27 domain, C-terminal | 12 - 63 | IPR014775-1 |
| domain | L27 domain, C-terminal | 69 - 120 | IPR014775-2 |
| conserved_site | Guanylate kinase, conserved site | 385 - 402 | IPR020590 |
| domain | MPP2, SH3 domain | 229 - 287 | IPR035602 |
Functions
| Description | ||
|---|---|---|
| EC Number | 2.7.12.2 | Dual-specificity kinases (those acting on Ser/Thr and Tyr residues) |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
No GO annotations of cellular component
| Name | Definition |
|---|---|
| No GO annotations for cellular component |
7 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| MAP kinase kinase activity | Catalysis of the concomitant phosphorylation of threonine (T) and tyrosine (Y) residues in a Thr-Glu-Tyr (TEY) thiolester sequence in a MAP kinase (MAPK) substrate. |
| protein serine kinase activity | Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate. |
| protein serine/threonine kinase activity | Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate. |
| protein serine/threonine/tyrosine kinase activity | Catalysis of the reactions: ATP + a protein serine = ADP + protein serine phosphate; ATP + a protein threonine = ADP + protein threonine phosphate; and ATP + a protein tyrosine = ADP + protein tyrosine phosphate. |
| protein tyrosine kinase activity | Catalysis of the reaction: ATP + a protein tyrosine = ADP + protein tyrosine phosphate. |
| scaffold protein binding | Binding to a scaffold protein. Scaffold proteins are crucial regulators of many key signaling pathways. Although not strictly defined in function, they are known to interact and/or bind with multiple members of a signaling pathway, tethering them into complexes. |
7 GO annotations of biological process
| Name | Definition |
|---|---|
| defense response to Gram-positive bacterium | Reactions triggered in response to the presence of a Gram-positive bacterium that act to protect the cell or organism. |
| MAPK cascade | An intracellular protein kinase cascade containing at least a MAPK, a MAPKK and a MAP3K. The cascade can also contain an additional tiers: the upstream MAP4K. The kinases in each tier phosphorylate and activate the kinase in the downstream tier to transmit a signal within a cell. |
| meiotic cell cycle | Progression through the phases of the meiotic cell cycle, in which canonically a cell replicates to produce four offspring with half the chromosomal content of the progenitor cell via two nuclear divisions. |
| oogenesis | The complete process of formation and maturation of an ovum or female gamete from a primordial female germ cell. Examples of this process are found in Mus musculus and Drosophila melanogaster. |
| protein phosphorylation | The process of introducing a phosphate group on to a protein. |
| Ras protein signal transduction | The series of molecular signals within the cell that are mediated by a member of the Ras superfamily of proteins switching to a GTP-bound active state. |
| vulval development | The process whose specific outcome is the progression of the egg-laying organ of female and hermaphrodite nematodes over time, from its formation to the mature structure. In nematodes, the vulva is formed from ventral epidermal cells during larval stages to give rise to a fully formed vulva in the adult. |
22 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P32491 | MKK2 | MAP kinase kinase MKK2/SSP33 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| P32490 | MKK1 | MAP kinase kinase MKK1/SSP32 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| Q90891 | MAP2K2 | Dual specificity mitogen-activated protein kinase kinase 2 | Gallus gallus (Chicken) | PR |
| Q9XT09 | MAP2K1 | Dual specificity mitogen-activated protein kinase kinase 1 | Pan troglodytes (Chimpanzee) | PR |
| Q1HG70 | MAP2K2 | Dual specificity mitogen-activated protein kinase kinase 2 | Canis lupus familiaris (Dog) (Canis familiaris) | PR |
| Q24324 | Dsor1 | Dual specificity mitogen-activated protein kinase kinase dSOR1 | Drosophila melanogaster (Fruit fly) | PR |
| Q13163 | MAP2K5 | Dual specificity mitogen-activated protein kinase kinase 5 | Homo sapiens (Human) | PR |
| P36507 | MAP2K2 | Dual specificity mitogen-activated protein kinase kinase 2 | Homo sapiens (Human) | EV |
| Q02750 | MAP2K1 | Dual specificity mitogen-activated protein kinase kinase 1 | Homo sapiens (Human) | EV |
| Q9WVS7 | Map2k5 | Dual specificity mitogen-activated protein kinase kinase 5 | Mus musculus (Mouse) | PR |
| P31938 | Map2k1 | Dual specificity mitogen-activated protein kinase kinase 1 | Mus musculus (Mouse) | PR |
| Q63932 | Map2k2 | Dual specificity mitogen-activated protein kinase kinase 2 | Mus musculus (Mouse) | PR |
| Q62862 | Map2k5 | Dual specificity mitogen-activated protein kinase kinase 5 | Rattus norvegicus (Rat) | PR |
| P36506 | Map2k2 | Dual specificity mitogen-activated protein kinase kinase 2 | Rattus norvegicus (Rat) | SS |
| Q01986 | Map2k1 | Dual specificity mitogen-activated protein kinase kinase 1 | Rattus norvegicus (Rat) | PR |
| Q5QN75 | MKK1 | Mitogen-activated protein kinase kinase 1 | Oryza sativa subsp japonica (Rice) | PR |
| G5EDT6 | jkk-1 | Dual specificity mitogen-activated protein kinase kinase jkk-1 | Caenorhabditis elegans | PR |
| G5EDF7 | sek-1 | Dual specificity mitogen-activated protein kinase kinase sek-1 | Caenorhabditis elegans | PR |
| Q21307 | mek-1 | Dual specificity mitogen-activated protein kinase kinase mek-1 | Caenorhabditis elegans | PR |
| Q9FJV0 | MKK6 | Mitogen-activated protein kinase kinase 6 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9S7U9 | MKK2 | Mitogen-activated protein kinase kinase 2 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q94A06 | MKK1 | Mitogen-activated protein kinase kinase 1 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSSGKRRNPL | GLSLPPTVNE | QSESGEATAE | EATATVPLEE | QLKKLGLTEP | QTQRLSEFLQ |
| 70 | 80 | 90 | 100 | 110 | 120 |
| VKEGIKELSE | DMLQTEGELG | HGNGGVVNKC | VHRKTGVIMA | RKLVHLEIKP | SVRQQIVKEL |
| 130 | 140 | 150 | 160 | 170 | 180 |
| AVLHKCNSPF | IVGFYGAFVD | NNDISICMEY | MDGLSLDIVL | KKVGRLPEKF | VGRISVAVVR |
| 190 | 200 | 210 | 220 | 230 | 240 |
| GLTYLKDEIK | ILHRDVKPSN | MLVNSNGEIK | LCDFGVSGML | IDSMANSFVG | TRSYMAPERL |
| 250 | 260 | 270 | 280 | 290 | 300 |
| TGSHYTISSD | IWSFGLSLVE | LLIGRYPVPA | PSQAEYATMF | NVAENEIELA | DSLEEPNYHP |
| 310 | 320 | 330 | 340 | 350 | 360 |
| PSNPASMAIF | EMLDYIVNGP | PPTLPKRFFT | DEVIGFVSKC | LRKLPSERAT | LKSLTADVFF |
| 370 | 380 | ||||
| TQYADHDDQG | EFAVFVKGTI | NLPKLNP |