Q0V9K5

SS
Gene name
rfx3
Protein name
Transcription factor RFX3
Names
Regulatory factor X 3
Species
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
KEGG Pathway
xtr:779608
EC number
Protein Class

Descriptions

RFX1 is a regulatory factor essential for MHC class II genes expression, and binds to the X boxes of MHC class II genes. This protein possesses intrinsically disordered regions (IDRs) with large negative charge, some of which involve a consecutive sequence of aspartate (D) or glutamate (E) residues, known as D/E repeats. These D/E repeats can cause autoinhibition through intramolecular electrostatic interaction with HMG boxes and modulate binding to DNA. This autoinhibited state can transition into the uninhibited complex with DNA through an electrostatically driven induced-fit process, which accelerates the target DNA search kinetics in the presence of non-functional high-affinity ligands ('decoys').

Autoinhibitory domains (AIDs)

670-682 (D/E repeat) SS

Target domain

113-272 (DNA binding domain)

Relief mechanism

PTM

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q0V9K5

Entry ID Method Resolution Chain Position Source
AF-Q0V9K5-F1 Predicted AlphaFoldDB

No variants for Q0V9K5

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q0V9K5

No associated diseases with Q0V9K5

2 regional properties for Q0V9K5

Type Name Position InterPro Accession
domain DNA-binding RFX-type winged-helix domain 183 - 258 IPR003150
domain RFX1 transcription activation region 25 - 134 IPR007668

Functions

Description
EC Number
Subcellular Localization
  • Nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
extracellular region The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

3 GO annotations of molecular function

Name Definition
DNA-binding transcription factor activity, RNA polymerase II-specific A DNA-binding transcription factor activity that modulates the transcription of specific gene sets transcribed by RNA polymerase II.
RNA polymerase II cis-regulatory region sequence-specific DNA binding Binding to a specific upstream regulatory DNA sequence (transcription factor recognition sequence or binding site) located in cis relative to the transcription start site (i.e., on the same strand of DNA) of a gene transcribed by RNA polymerase II.
transcription cis-regulatory region binding Binding to a specific sequence of DNA that is part of a regulatory region that controls transcription of that section of the DNA. The transcribed region might be described as a gene, cistron, or operon.

7 GO annotations of biological process

Name Definition
cell differentiation The cellular developmental process in which a relatively unspecialized cell, e.g. embryonic or regenerative cell, acquires specialized structural and/or functional features that characterize a specific cell. Differentiation includes the processes involved in commitment of a cell to a specific fate and its subsequent development to the mature state.
cilium-dependent cell motility Cell motility due to the motion of one or more eukaryotic cilia. A eukaryotic cilium is a specialized organelle that consists of a filiform extrusion of the cell surface. Each cilium is bounded by an extrusion of the cytoplasmic (plasma) membrane, and contains a regular longitudinal array of microtubules, anchored basally in a centriole.
DNA-templated transcription The synthesis of an RNA transcript from a DNA template.
endocrine pancreas development The process whose specific outcome is the progression of the endocrine pancreas over time, from its formation to the mature structure. The endocrine pancreas is made up of islet cells that produce insulin, glucagon and somatostatin.
epithelial cilium movement involved in determination of left/right asymmetry The movement of cilia of epithelial cells of the Left Right Organizer (LRO), also referred to as the node in mouse or the Kupffer's vesicle in zebrafish, resulting in the leftward fluid flow across the LRO and generation or transport of a signal which determines asymmetry in an organism's body plan with respect to the left and right halves.
regulation of DNA-templated transcription Any process that modulates the frequency, rate or extent of cellular DNA-templated transcription.
regulation of insulin secretion Any process that modulates the frequency, rate or extent of the regulated release of insulin.

5 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P22670 RFX1 MHC class II regulatory factor RFX1 Homo sapiens (Human) EV
P48378 RFX2 DNA-binding protein RFX2 Homo sapiens (Human) SS
P48380 RFX3 Transcription factor RFX3 Homo sapiens (Human) SS
P48377 Rfx1 MHC class II regulatory factor RFX1 Mus musculus (Mouse) SS
P48381 Rfx3 Transcription factor RFX3 Mus musculus (Mouse) SS
10 20 30 40 50 60
MQTSETVSDT GSTVTLQTSV AGQAAVPTQV VQQVPVQQQV QQVQTVQQVQ HVYPAQVQYV
70 80 90 100 110 120
EGSDTVYTNG AIRTTTYPYT ETQMYSQNTG GNYFDTQGGS AQVTTVVSTH SMVGTGGIQM
130 140 150 160 170 180
GVTGGQIISS TGGTYLIGNA MENSGHSVSH TTRASPATIE MAIETLQKSD GLSSHRSSLL
190 200 210 220 230 240
NSHLQWLLDN YETAEGVSLP RSTLYNHYLR HCQEHKLDPV NAASFGKLIR SIFMGLRTRR
250 260 270 280 290 300
LGTRGNSKYH YYGIRVKPDS PLNRLQEDMQ YMAMRQQPMQ QKQRYKPMQK VDGVGDGFAG
310 320 330 340 350 360
SGQSGASVEQ TVIAQSQHHQ QFLDASRALP EFAEVEISSL PDGTTFEDIK SLQSLYREHC
370 380 390 400 410 420
EAILDVVVNL QFSLIEKLWQ TFWRYSPSSP ADGSTITESG NLSEIESRLP KSKLILFCKN
430 440 450 460 470 480
ESIVKWMCNC DHMMYQSLVE ILIPDVLRPI PSALTQAVRN FAKSLEGWLS SAMSNIPQRM
490 500 510 520 530 540
IQTKVAAVSA FAQTLRRYTS LNHLAQAARA VLQNTSQINQ MLNDLNRVDF ANVQEQASWV
550 560 570 580 590 600
CQCDDSMVQR LETDFKMTLQ QQSTLEQWAA WLDNVVTQAL KPYEGRPSFP KAARQFLLKW
610 620 630 640 650 660
SFYSSMVIRD LTLRSAASFG SFHLIRLLYD EYMFYLVEHR VAQATGESPI AVMGEFGDLN
670 680 690 700 710 720
DASPGNMEKD EGSDVESEIE EELDDSVEPP AKREKTELSQ AFQVGCMQPS LEGSVQQSLV
730 740
LNQLHSEHIV TSTQTIRQCS ATGNTYTAV