Q0G819
SS
Gene name |
tax-6 (cna-1, C02F4.2) |
Protein name |
Serine/threonine-protein phosphatase 2B catalytic subunit |
Names |
EC 3.1.3.16 , Abnormal chemotaxis protein 6 , Calmodulin-dependent calcineurin subunit A |
Species |
Caenorhabditis elegans |
KEGG Pathway |
cel:CELE_C02F4.2 |
EC number |
3.1.3.16: Phosphoric monoester hydrolases |
Protein Class |
|
Descriptions
Calcium-dependent, calmodulin-stimulated protein phosphatase calcineurin (CN) plays a role in the transduction of intracellular Ca2+-dependent signals. CN is a heterodimer composed of a catalytic subunit A (CNA) and an essential regulatory subunit B (CNB). <br>CNA contains two autoinhibitory regions, which interfere with the function of the catalytic domain: autoinhibitory segment (AIS) and autoinhibitory domain (AID). The AIS interacts with a hydrophobic intersubunit groove formed at the junction of CNA and CNB, and disruption of the AIS interaction results in partial stimulation of CN activity. In addition, the binding partner calmodulin regulates the orientation of AID with respect to the catalytic core, resulting in incomplete activation of CN.
Autoinhibitory domains (AIDs)
Target domain |
118-318 (Calcineurin-like phosphoesterase domain) |
Relief mechanism |
Partner binding |
Assay |
|
Target domain |
118-318 (Calcineurin-like phosphoesterase domain) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
No accessory elements
References
- Tokoyoda K et al. (2000) "Synergism between the calmodulin-binding and autoinhibitory domains on calcineurin is essential for the induction of their phosphatase activity", The Journal of biological chemistry, 275, 11728-34
- Li SJ et al. (2016) "Cooperative autoinhibition and multi-level activation mechanisms of calcineurin", Cell research, 26, 336-49
- Rumi-Masante J et al. (2012) "Structural basis for activation of calcineurin by calmodulin", Journal of molecular biology, 415, 307-17
- Kissinger CR et al. (1995) "Crystal structures of human calcineurin and the human FKBP12-FK506-calcineurin complex", Nature, 378, 641-4
- Ye Q et al. (2008) "The complex structure of calmodulin bound to a calcineurin peptide", Proteins, 73, 19-27
Autoinhibited structure
Activated structure
1 structures for Q0G819
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q0G819-F1 | Predicted | AlphaFoldDB |
No variants for Q0G819
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for Q0G819 | |||||
No associated diseases with Q0G819
3 regional properties for Q0G819
Functions
| Description | ||
|---|---|---|
| EC Number | 3.1.3.16 | Phosphoric monoester hydrolases |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
8 GO annotations of cellular component
| Name | Definition |
|---|---|
| axon | The long process of a neuron that conducts nerve impulses, usually away from the cell body to the terminals and varicosities, which are sites of storage and release of neurotransmitter. |
| calcineurin complex | A heterodimeric calcium ion and calmodulin dependent protein phosphatase composed of catalytic and regulatory subunits; the regulatory subunit is very similar in sequence to calmodulin. |
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| dendrite | A neuron projection that has a short, tapering, morphology. Dendrites receive and integrate signals from other neurons or from sensory stimuli, and conduct nerve impulses towards the axon or the cell body. In most neurons, the impulse is conveyed from dendrites to axon via the cell body, but in some types of unipolar neuron, the impulse does not travel via the cell body. |
| neuronal cell body | The portion of a neuron that includes the nucleus, but excludes cell projections such as axons and dendrites. |
| non-motile cilium | A cilium which may have a variable array of axonemal microtubules but does not contain molecular motors. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| perikaryon | The portion of the cell soma (neuronal cell body) that excludes the nucleus. |
5 GO annotations of molecular function
| Name | Definition |
|---|---|
| calcium ion binding | Binding to a calcium ion (Ca2+). |
| calmodulin binding | Binding to calmodulin, a calcium-binding protein with many roles, both in the calcium-bound and calcium-free states. |
| calmodulin-dependent protein phosphatase activity | Catalysis of the reaction |
| myosin phosphatase activity | Catalysis of the reaction |
| phosphoprotein phosphatase activity | Catalysis of the reaction |
17 GO annotations of biological process
| Name | Definition |
|---|---|
| calcineurin-mediated signaling | Any intracellular signal transduction in which the signal is passed on within the cell by activation of a transcription factor as a consequence of dephosphorylation by Ca(2+)-activated calcineurin. The process begins with calcium-dependent activation of the phosphatase calcineurin. Calcineurin is a calcium- and calmodulin-dependent serine/threonine protein phosphatase with a conserved function in eukaryotic species from yeast to humans. In yeast and fungi, calcineurin regulates stress signaling and cell cycle, and sporulation and virulence in pathogenic fungi. In metazoans, calcineurin is involved in cell commitment, organogenesis and organ development and immune function of T-lymphocytes. By a conserved mechanism, calcineurin phosphatase activates fungal Crz1 and mammalian NFATc by dephosphorylation and translocation of these transcription factors to the nucleus to regulate gene expression. |
| calcium-mediated signaling | Any intracellular signal transduction in which the signal is passed on within the cell via calcium ions. |
| chemosensory behavior | Behavior that is dependent upon the sensation of chemicals. |
| chemotaxis | The directed movement of a motile cell or organism, or the directed growth of a cell guided by a specific chemical concentration gradient. Movement may be towards a higher concentration (positive chemotaxis) or towards a lower concentration (negative chemotaxis). |
| dauer larval development | The process whose specific outcome is the progression of the dauer larva over time, through the facultative diapause of the dauer (enduring) larval stage, with specialized traits adapted for dispersal and long-term survival, with elevated stress resistance and without feeding. |
| determination of adult lifespan | The pathways that regulate the duration of the adult phase of the life-cycle of an animal. |
| endocytosis | A vesicle-mediated transport process in which cells take up external materials or membrane constituents by the invagination of a part of the plasma membrane to form a new membrane-bounded vesicle. |
| hyperosmotic response | Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of detection of, or exposure to, a hyperosmotic environment, i.e. an environment with a higher concentration of solutes than the organism or cell. |
| olfactory behavior | The behavior of an organism in response to an odor. |
| olfactory learning | Any process in an organism in which a relatively long-lasting adaptive behavioral change occurs in response to (repeated) exposure to an olfactory cue. |
| protein localization to non-motile cilium | A process in which a protein is transported to, or maintained in, a location within a non-motile cilium. |
| regulation of gene expression | Any process that modulates the frequency, rate or extent of gene expression. Gene expression is the process in which a gene's coding sequence is converted into a mature gene product (protein or RNA). |
| response to alkaline pH | Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a pH stimulus with pH > 7. pH is a measure of the acidity or basicity of an aqueous solution. |
| response to hermaphrodite contact | The response by the male to a hermaphrodite after initial contact following mate finding. The male stops forward locomotion, presses the ventral side of his tail against his partner's body, and begins moving backward along the hermaphrodite. Male response behavior is initiated when sensory neurons located in the rays of his tail contact a potential mate. |
| thermosensory behavior | Behavior that is dependent upon the sensation of temperature. |
| thermotaxis | The directed movement of a motile cell or organism in response to a temperature gradient. Movement may be towards either a higher or lower temperature. |
| vulval location | Location, by the male, of his partner's vulva when backing along the ventral side of the hermaphrodite during mating. The male stops at the vulva, coordinates his movements to the hermaphrodite's, and positions his tail precisely over the vulva so that he may insert his spicules and ejaculate. |
13 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P23287 | CNA1 | Serine/threonine-protein phosphatase 2B catalytic subunit A1 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| P48452 | PPP3CA | Protein phosphatase 3 catalytic subunit alpha | Bos taurus (Bovine) | SS |
| P48456 | CanA1 | Serine/threonine-protein phosphatase 2B catalytic subunit 1 | Drosophila melanogaster (Fruit fly) | SS |
| Q27889 | Pp2B-14D | Serine/threonine-protein phosphatase 2B catalytic subunit 2 | Drosophila melanogaster (Fruit fly) | SS |
| Q9VXF1 | CanA-14F | Serine/threonine-protein phosphatase 2B catalytic subunit 3 | Drosophila melanogaster (Fruit fly) | SS |
| P48454 | PPP3CC | Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform | Homo sapiens (Human) | SS |
| P16298 | PPP3CB | Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform | Homo sapiens (Human) | EV |
| Q08209 | PPP3CA | Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform | Homo sapiens (Human) | EV |
| P48455 | Ppp3cc | Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform | Mus musculus (Mouse) | PR |
| P48453 | Ppp3cb | Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform | Mus musculus (Mouse) | SS |
| P63328 | Ppp3ca | Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform | Mus musculus (Mouse) | EV |
| P20651 | Ppp3cb | Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform | Rattus norvegicus (Rat) | SS |
| P63329 | Ppp3ca | Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform | Rattus norvegicus (Rat) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MASTSAGQNS | AAKPDTTNTT | TTASNNNRER | ERDLKQFTER | FVKTVQFPVS | ERLTVDQVYD |
| 70 | 80 | 90 | 100 | 110 | 120 |
| RRTGKPRHEV | LRDHFIKEGR | IEEEAAIRVI | QECSSLFRNE | KTMLEIEAPV | TVCGDIHGQF |
| 130 | 140 | 150 | 160 | 170 | 180 |
| YDLMKLFEVG | GSPATTKYLF | LGDYVDRGYF | SIECVLYLWA | LKICYPTTLF | LLRGNHECRH |
| 190 | 200 | 210 | 220 | 230 | 240 |
| LTEYFTFKQE | CKIKYSERVY | DVCMESFDAL | PLAALMNQQF | LCVHGGLSPE | IHTLEDIRRI |
| 250 | 260 | 270 | 280 | 290 | 300 |
| DRFKEPPAFG | PMCDLLWSDP | LEDFGNERNS | EQFSHNSVRG | CSYFYSYAAC | CDFLQHNNLL |
| 310 | 320 | 330 | 340 | 350 | 360 |
| SIIRAHEAQD | AGYRMYRKSQ | ATGFPSLITI | FSAPNYLDVY | NNKAAILKYE | NNVMNIRQFN |
| 370 | 380 | 390 | 400 | 410 | 420 |
| CSPHPYWLPN | FMDVFTWSLP | FVGEKVTEML | VHILNICSDD | ELMAECDDTF | EGGVGSARKE |
| 430 | 440 | 450 | 460 | 470 | 480 |
| VIRHKIRAIG | KMARAFSVLR | HESESVLQLK | GLNAGGKLPQ | GALSEGRTGL | NAAYRIEQSV |
| 490 | 500 | 510 | 520 | 530 | 540 |
| AAESGCDSGH | LIQSFEEARR | LDKINERMPP | TMATPPAQSP | SQSPIPSRQT | TPQPPQNGPS |
| NS |