Q09136
SS
Gene name |
PRKAA1 (AMPK1) |
Protein name |
5'-AMP-activated protein kinase catalytic subunit alpha-1 |
Names |
AMPK subunit alpha-1 , EC 2.7.11.1 , AMPK 63 kDa subunit , Acetyl-CoA carboxylase kinase , ACACA kinase , Hydroxymethylglutaryl-CoA reductase kinase , HMGCR kinase , EC 2.7.11.31 , Tau-protein kinase PRKAA1 , EC 2.7.11.26 |
Species |
Sus scrofa (Pig) |
KEGG Pathway |
|
EC number |
2.7.11.1: Protein-serine/threonine kinases |
Protein Class |
|
Descriptions
The AMP-activated protein kinase (AMPK) is characterized by its ability to bind to AMP, which enables it to adjust enzymatic activity by sensing the cellular energy status and maintain the balance between ATP production and consumption in eukaryotic cells. The autoinhibitory domain (AID) binds to the hinge region of its kinase domain (KD), forming contacts with both amino-terminal and carboxy-terminal lobes. AID binding can restrict the mobility of helix αC, resulting in an autoinhibited KD-AID with much lower kinase activity than that of the KD alone. AMP activates AMPK both allosterically and by inhibiting dephosphorylation.
Autoinhibitory domains (AIDs)
Target domain |
9-229 (Protein kinase domain) |
Relief mechanism |
Ligand binding |
Assay |
|
Accessory elements
117-139 (Activation loop from InterPro)
Target domain |
1-229 (Protein kinase domain) |
Relief mechanism |
|
Assay |
|
References
- Pang T et al. (2007) "Conserved alpha-helix acts as autoinhibitory sequence in AMP-activated protein kinase alpha subunits", The Journal of biological chemistry, 282, 495-506
- Hardie DG et al. (2012) "AMP-activated protein kinase: a target for drugs both ancient and modern", Chemistry & biology, 19, 1222-36
- Li YY et al. (2013) "Novel small-molecule AMPK activator orally exerts beneficial effects on diabetic db/db mice", Toxicology and applied pharmacology, 273, 325-34
- Chen L et al. (2009) "Structural insight into the autoinhibition mechanism of AMP-activated protein kinase", Nature, 459, 1146-9
Autoinhibited structure
Activated structure
0 structures for Q09136
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|
No variants for Q09136
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for Q09136 | |||||
No associated diseases with Q09136
5 regional properties for Q09136
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | PAS domain | 220 - 324 | IPR000014 |
| domain | 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain | 480 - 820 | IPR002073 |
| domain | HD/PDEase domain | 553 - 749 | IPR003607 |
| domain | PAS fold | 219 - 324 | IPR013767 |
| conserved_site | 3'5'-cyclic nucleotide phosphodiesterase, conserved site | 596 - 607 | IPR023174 |
Functions
| Description | ||
|---|---|---|
| EC Number | 2.7.11.1 | Protein-serine/threonine kinases |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
3 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| nucleotide-activated protein kinase complex | A protein complex that possesses nucleotide-dependent protein kinase activity. The nucleotide can be AMP (in S. pombe and human) or ADP (in S. cerevisiae). |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
8 GO annotations of molecular function
| Name | Definition |
|---|---|
| AMP-activated protein kinase activity | Catalysis of the reaction |
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| chromatin binding | Binding to chromatin, the network of fibers of DNA, protein, and sometimes RNA, that make up the chromosomes of the eukaryotic nucleus during interphase. |
| histone H2BS36 kinase activity | Catalysis of the reaction |
| metal ion binding | Binding to a metal ion. |
| protein serine kinase activity | Catalysis of the reactions |
| protein serine/threonine kinase activity | Catalysis of the reactions |
| tau-protein kinase activity | Catalysis of the reaction |
24 GO annotations of biological process
| Name | Definition |
|---|---|
| autophagy | The cellular catabolic process in which cells digest parts of their own cytoplasm; allows for both recycling of macromolecular constituents under conditions of cellular stress and remodeling the intracellular structure for cell differentiation. |
| cellular response to glucose starvation | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of deprivation of glucose. |
| cellular response to nutrient levels | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus reflecting the presence, absence, or concentration of nutrients. |
| cholesterol biosynthetic process | The chemical reactions and pathways resulting in the formation of cholesterol, cholest-5-en-3 beta-ol, the principal sterol of vertebrates and the precursor of many steroids, including bile acids and steroid hormones. |
| energy homeostasis | Any process involved in the balance between food intake (energy input) and energy expenditure. |
| fatty acid biosynthetic process | The chemical reactions and pathways resulting in the formation of a fatty acid, any of the aliphatic monocarboxylic acids that can be liberated by hydrolysis from naturally occurring fats and oils. Fatty acids are predominantly straight-chain acids of 4 to 24 carbon atoms, which may be saturated or unsaturated; branched fatty acids and hydroxy fatty acids also occur, and very long chain acids of over 30 carbons are found in waxes. |
| fatty acid homeostasis | Any process involved in the maintenance of an internal steady state of fatty acid within an organism or cell. |
| glucose homeostasis | Any process involved in the maintenance of an internal steady state of glucose within an organism or cell. |
| intracellular signal transduction | The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell. |
| lipid biosynthetic process | The chemical reactions and pathways resulting in the formation of lipids, compounds soluble in an organic solvent but not, or sparingly, in an aqueous solvent. |
| lipid droplet disassembly | The disaggregation of a lipid particle into its constituent components. |
| negative regulation of apoptotic process | Any process that stops, prevents, or reduces the frequency, rate or extent of cell death by apoptotic process. |
| negative regulation of hepatocyte apoptotic process | Any process that stops, prevents or reduces the frequency, rate or extent of hepatocyte apoptotic process. |
| negative regulation of lipid catabolic process | Any process that stops, prevents, or reduces the frequency, rate or extent of the chemical reactions and pathways resulting in the breakdown of lipids. |
| negative regulation of TOR signaling | Any process that stops, prevents, or reduces the frequency, rate or extent of TOR signaling. |
| negative regulation of TORC1 signaling | Any process that stops, prevents or reduces the frequency, rate or extent of TORC1 signaling. |
| phosphorylation | The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide. |
| positive regulation of autophagy | Any process that activates, maintains or increases the rate of autophagy. Autophagy is the process in which cells digest parts of their own cytoplasm. |
| positive regulation of glycolytic process | Any process that activates or increases the frequency, rate or extent of glycolysis. |
| protein localization to lipid droplet | A process in which a protein is transported to, or maintained in, a location on or within a lipid droplet. |
| regulation of circadian rhythm | Any process that modulates the frequency, rate or extent of a circadian rhythm. A circadian rhythm is a biological process in an organism that recurs with a regularity of approximately 24 hours. |
| response to gamma radiation | Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a gamma radiation stimulus. Gamma radiation is a form of electromagnetic radiation (EMR) or light emission of a specific frequency produced from sub-atomic particle interaction, such as electron-positron annihilation and radioactive decay. Gamma rays are generally characterized as EMR having the highest frequency and energy, and also the shortest wavelength, within the electromagnetic radiation spectrum. |
| rhythmic process | Any process pertinent to the generation and maintenance of rhythms in the physiology of an organism. |
| Wnt signaling pathway | The series of molecular signals initiated by binding of a Wnt protein to a frizzled family receptor on the surface of the target cell and ending with a change in cell state. |
20 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P06782 | SNF1 | Carbon catabolite-derepressing protein kinase | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | EV |
| Q13131 | PRKAA1 | 5'-AMP-activated protein kinase catalytic subunit alpha-1 | Homo sapiens (Human) | EV |
| Q5EG47 | Prkaa1 | 5'-AMP-activated protein kinase catalytic subunit alpha-1 | Mus musculus (Mouse) | SS |
| Q28948 | PRKAA2 | 5'-AMP-activated protein kinase catalytic subunit alpha-2 | Sus scrofa (Pig) | SS |
| P54645 | Prkaa1 | 5'-AMP-activated protein kinase catalytic subunit alpha-1 | Rattus norvegicus (Rat) | EV |
| Q852Q2 | OSK1 | Serine/threonine protein kinase OSK1 | Oryza sativa subsp. japonica (Rice) | SS |
| Q852Q1 | OSK4 | Serine/threonine protein kinase OSK4 | Oryza sativa subsp. japonica (Rice) | SS |
| Q852Q0 | OSK3 | Serine/threonine protein kinase OSK3 | Oryza sativa subsp. japonica (Rice) | SS |
| Q8LIG4 | CIPK3 | CBL-interacting protein kinase 3 | Oryza sativa subsp japonica (Rice) | PR |
| Q2RAX3 | CIPK33 | CBL-interacting protein kinase 33 | Oryza sativa subsp japonica (Rice) | PR |
| Q2QY53 | CIPK32 | CBL-interacting protein kinase 32 | Oryza sativa subsp japonica (Rice) | PR |
| Q6ZLP5 | CIPK23 | CBL-interacting protein kinase 23 | Oryza sativa subsp japonica (Rice) | PR |
| P45894 | aak-1 | 5'-AMP-activated protein kinase catalytic subunit alpha-1 | Caenorhabditis elegans | SS |
| Q95ZQ4 | aak-2 | 5'-AMP-activated protein kinase catalytic subunit alpha-2 | Caenorhabditis elegans | SS |
| Q9FLZ3 | KIN12 | SNF1-related protein kinase catalytic subunit alpha KIN12 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q94CG0 | CIPK21 | CBL-interacting serine/threonine-protein kinase 21 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q93VD3 | CIPK23 | CBL-interacting serine/threonine-protein kinase 23 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9LDI3 | CIPK24 | CBL-interacting serine/threonine-protein kinase 24 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q38997 | KIN10 | SNF1-related protein kinase catalytic subunit alpha KIN10 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| P92958 | KIN11 | SNF1-related protein kinase catalytic subunit alpha KIN11 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| DGRVKIGHYI | LGDTLGVGTF | GKRREIQNLK | LFRHPHIIKL | YQVISTPSDI | FMVMEYVSGG |
| 70 | 80 | 90 | 100 | 110 | 120 |
| ELFDYICKNG | RLDEKESRRL | FQQILSGVDY | CHRHMVVHRD | LKPENVLLDA | HMNAKIADFG |
| 130 | 140 | 150 | 160 | 170 | 180 |
| LSNMMSDGEF | LRTSCGSPNY | AAPEVISGRL | YAGPEVDIWS | SGVILYALLC | GTLPFDDDHV |
| 190 | 200 | 210 | 220 | 230 | 240 |
| PTLFKKICDG | IFYTPQYLNP | SVISLLKHML | QVDPMKRATI | KDIREHEWFK | QDLPKYLFPE |
| 250 | 260 | 270 | 280 | 290 | 300 |
| DPSYSXTMID | DEALKQDPLA | VAYHLIIDNR | DFYLATSPPD | SFLDDHHLTR | VPFLVAETPR |
| 310 | 320 | 330 | 340 | 350 | 360 |
| DELNPQKXKH | QGVRKAKXHL | GIRQLDYEXK | VVNPYYLRVR | RKKMSLQLYQ | VDSRTYLLDF |
| 370 | 380 | ||||
| RSIDDXIDAE | AQGKSSEASL | TXSVT |