Q08BS4
SS
Gene name |
ezh2 (zgc:152758) |
Protein name |
Histone-lysine N-methyltransferase EZH2 |
Names |
EC 2.1.1.356 , Enhancer of zeste homolog 2 |
Species |
Danio rerio (Zebrafish) (Brachydanio rerio) |
KEGG Pathway |
dre:768133 |
EC number |
2.1.1.356: Methyltransferases |
Protein Class |
|
Descriptions
The histone-lysine N-methyltransferase EZH2 (EZH2) is a catalytic subunit of the polycomb repressive complex 2 (PRC2) and is involved in histone methylation and transcriptional repression. Autoinhibition of EZH2 is achieved through the C-terminus folding back into the active site, effectively blocking substrate engagement. This conformational state is inactive, preventing enzymatic activity and ensuring EZH2 only functions when properly complexed, preventing aberrant methylation activity. The inhibited activity of EZH2 could be alleviated by interactions with its binding partners EED and SUZ12, which could induce a conformational change in EZH2
Autoinhibitory domains (AIDs)
Target domain |
660-745 (Active sites of SET domain) |
Relief mechanism |
Partner binding, Ligand binding |
Assay |
|
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
1 structures for Q08BS4
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q08BS4-F1 | Predicted | AlphaFoldDB |
No variants for Q08BS4
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for Q08BS4 | |||||
No associated diseases with Q08BS4
9 regional properties for Q08BS4
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | SANT/Myb domain | 160 - 263 | IPR001005-1 |
| domain | SANT/Myb domain | 442 - 490 | IPR001005-2 |
| domain | SET domain | 626 - 747 | IPR001214 |
| domain | CXC domain | 517 - 619 | IPR026489 |
| domain | Tesmin/TSO1-like CXC domain | 569 - 607 | IPR033467 |
| domain | Polycomb repressive complex 2 subunit EZH1/EZH2, tri-helical domain | 159 - 262 | IPR041343 |
| domain | Pre-SET CXC domain | 573 - 604 | IPR041355 |
| domain | EZH2, SET domain | 623 - 742 | IPR044439 |
| domain | EZH1/2, MCSS domain | 270 - 322 | IPR048358 |
Functions
| Description | ||
|---|---|---|
| EC Number | 2.1.1.356 | Methyltransferases |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
2 GO annotations of cellular component
| Name | Definition |
|---|---|
| ESC/E(Z) complex | A multimeric protein complex that can methylate lysine-27 and lysine-9 residues of histone H3. In Drosophila the core subunits of the complex include ESC, E(Z), CAF1 (NURF-55) and SU(Z)12. In mammals the core subunits of the complex include EED, EZH2, SUZ12 and RBBP4. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
6 GO annotations of molecular function
| Name | Definition |
|---|---|
| chromatin binding | Binding to chromatin, the network of fibers of DNA, protein, and sometimes RNA, that make up the chromosomes of the eukaryotic nucleus during interphase. |
| histone H3K27 methyltransferase activity | Catalysis of the reaction |
| histone H3K27 trimethyltransferase activity | Catalysis of the reaction |
| promoter-specific chromatin binding | Binding to a section of chromatin that is associated with gene promoter sequences of DNA. |
| transcription coactivator activity | A transcription coregulator activity that activates or increases the transcription of specific gene sets via binding to a DNA-bound DNA-binding transcription factor, either on its own or as part of a complex. Coactivators often act by altering chromatin structure and modifications. For example, one class of transcription coactivators modifies chromatin structure through covalent modification of histones. A second class remodels the conformation of chromatin in an ATP-dependent fashion. A third class modulates interactions of DNA-bound DNA-binding transcription factors with other transcription coregulators. A fourth class of coactivator activity is the bridging of a DNA-binding transcription factor to the general (basal) transcription machinery. The Mediator complex, which bridges sequence-specific DNA binding transcription factors and RNA polymerase, is also a transcription coactivator. |
| transcription coregulator activity | A transcription regulator activity that modulates the transcription of specific gene sets via binding to a DNA-bound DNA-binding transcription factor, either on its own or as part of a complex. Coregulators often act by altering chromatin structure and modifications. For example, one class of transcription coregulators modifies chromatin structure through covalent modification of histones. A second class remodels the conformation of chromatin in an ATP-dependent fashion. A third class modulates interactions of DNA-bound DNA-binding transcription factors with other transcription coregulators. |
10 GO annotations of biological process
| Name | Definition |
|---|---|
| circadian rhythm | Any biological process in an organism that recurs with a regularity of approximately 24 hours. |
| digestive tract development | The process whose specific outcome is the progression of the digestive tract over time, from its formation to the mature structure. The digestive tract is the anatomical structure through which food passes and is processed. |
| fin regeneration | The regrowth of fin tissue following its loss or destruction. |
| heart development | The process whose specific outcome is the progression of the heart over time, from its formation to the mature structure. The heart is a hollow, muscular organ, which, by contracting rhythmically, keeps up the circulation of the blood. |
| heterochromatin formation | An epigenetic gene silencing mechanism in which chromatin is compacted into heterochromatin, resulting in a chromatin conformation refractory to transcription. This process starts with heterochromatin nucleation, its spreading, and ends with heterochromatin boundary formation. |
| methylation | The process in which a methyl group is covalently attached to a molecule. |
| negative regulation of gene expression, epigenetic | An epigenetic process that silences gene expression at specific genomic regions through chromatin remodelling either by modifying higher order chromatin fiber structure, nucleosomal histones, or the DNA. |
| positive regulation of circadian rhythm | Any process that activates or increases the frequency, rate or extent of a circadian rhythm behavior. |
| positive regulation of hemopoiesis | Any process that activates or increases the frequency, rate or extent of hemopoiesis. |
| tissue homeostasis | A homeostatic process involved in the maintenance of an internal steady state within a defined tissue of an organism, including control of cellular proliferation and death and control of metabolic function. |
8 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| A7E2Z2 | EZH1 | Histone-lysine N-methyltransferase EZH1 | Bos taurus (Bovine) | SS |
| P42124 | E(z) | Histone-lysine N-methyltransferase E | Drosophila melanogaster (Fruit fly) | SS |
| Q92800 | EZH1 | Histone-lysine N-methyltransferase EZH1 | Homo sapiens (Human) | SS |
| Q15910 | EZH2 | Histone-lysine N-methyltransferase EZH2 | Homo sapiens (Human) | EV |
| P70351 | Ezh1 | Histone-lysine N-methyltransferase EZH1 | Mus musculus (Mouse) | SS |
| Q61188 | Ezh2 | Histone-lysine N-methyltransferase EZH2 | Mus musculus (Mouse) | SS |
| O65312 | MEA | Histone-lysine N-methyltransferase MEDEA | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q28D84 | ezh2 | Histone-lysine N-methyltransferase EZH2 | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MGLTGRKSEK | GPVCWRRRVK | SEYMRLRQLK | RFRRADEVKS | MFSSNRQKIL | ERTDILNQEW |
| 70 | 80 | 90 | 100 | 110 | 120 |
| KLRRIQPVHI | MTPVSSLRGT | RECTVDSGFS | EFSRQVIPLK | TLNAVASVPV | MYSWSPLQQN |
| 130 | 140 | 150 | 160 | 170 | 180 |
| FMVEDETVLH | NIPYMGDEIL | DQDGTFIEEL | IKNYDGKVHG | DRECGFINDE | IFVELVNALN |
| 190 | 200 | 210 | 220 | 230 | 240 |
| QYSDNEEDDE | EDDHHDYKFE | KMDLCDGKDD | AEDHKEQLSS | ESHNNDGSKK | FPSDKIFEAI |
| 250 | 260 | 270 | 280 | 290 | 300 |
| SSMFPDKGST | EELKEKYKEL | TEQQLPGALP | PECTPNIDGP | NAKSVQREQS | LHSFHTLFCR |
| 310 | 320 | 330 | 340 | 350 | 360 |
| RCFKYDCFLH | PFQATPNTYK | RKNMENLVDS | KPCGIYCYMY | MVQDGMVREY | PAGVVPERAK |
| 370 | 380 | 390 | 400 | 410 | 420 |
| TPSKRSTGRR | RGRLPNSNSR | PSTPTVNSET | KDTDSDREGG | ADGNDSNDKD | DDDKKDETTS |
| 430 | 440 | 450 | 460 | 470 | 480 |
| SSEANSRCQT | PVKLKLSSEP | PENVDWSGAE | ASLFRVLIGT | YYDNFCAIAR | LIGTKTCRQV |
| 490 | 500 | 510 | 520 | 530 | 540 |
| YEFRVKESSI | IARAPAVDEN | TPQRKKKRKH | RLWATHCRKI | QLKKDGSSNH | VYNYQPCDHP |
| 550 | 560 | 570 | 580 | 590 | 600 |
| RQPCDSSCPC | VTAQNFCEKF | CQCSSECQNR | FPGCRCKAQC | NTKQCPCYLA | VRECDPDLCL |
| 610 | 620 | 630 | 640 | 650 | 660 |
| TCGAAEHWDS | KNVSCKNCSI | QRGAKKHLLL | APSDVAGWGI | FIKEPVQKNE | FISEYCGEII |
| 670 | 680 | 690 | 700 | 710 | 720 |
| SQDEADRRGK | VYDKYMCSFL | FNLNNDFVVD | ATRKGNKIRF | ANHSVNPNCY | AKVMMVNGDH |
| 730 | 740 | 750 | |||
| RIGIFAKRAI | QTGEELFFDY | RYSQADALKY | VGIEREMEIP |