Q07832

EV
Gene name
Plk1 (Plk)
Protein name
Serine/threonine-protein kinase PLK1
Names
Polo-like kinase 1, PLK-1, Serine/threonine-protein kinase 13, STPK13
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:18817
EC number
2.7.11.21: Protein-serine/threonine kinases
Protein Class
SERINE/THREONINE-PROTEIN KINASE PLK (PTHR24345)

Descriptions

Polo-like kinase (Plk) acts at various stages in early and late mitosis and is involved in the regulation of centrosome maturation and spindle assembly. The C-terminal region of Plk1 regulates its kinase activity through intramolecular binding. The C-terminal region of Plk1, which contains the polo-box domain, occludes the catalytic kinase domain in the same protein and this autoinhibition is relieved by removal of the C-terminal region. In human PLK1 (Q07832), the polo-box also plays an autoinhibitory role.

Autoinhibitory domains (AIDs)

402-603 (Polo box domain) EV

Target domain

53-305 (Protein kinase domain)

Relief mechanism

PTM

Assay

Deletion assay, Mutagenesis experiment

Accessory elements

193-216 (Activation loop from InterPro)

Target domain

53-305 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

5 structures for Q07832

Entry ID Method Resolution Chain Position Source
5DMS X-ray 190 A A/C 367-603 PDB
5DMV X-ray 250 A C 367-603 PDB
5DNJ X-ray 230 A A 367-603 PDB
5X3S X-ray 290 A A/B 371-594 PDB
AF-Q07832-F1 Predicted AlphaFoldDB

5 variants for Q07832

Variant ID(s) Position Change Description Diseaes Association Provenance
rs1132151208 26 A>V No Ensembl
rs1134340264 63 G>R No Ensembl
rs1131751745 313 R>C No Ensembl
rs239855953 364 R>W No Ensembl
rs1134052353 562 Y>N No Ensembl

No associated diseases with Q07832

2 regional properties for Q07832

Type Name Position InterPro Accession
domain MerR-type HTH domain 1 - 72 IPR000551
domain TipAS antibiotic-recognition domain 131 - 247 IPR012925

Functions

Description
EC Number 2.7.11.21 Protein-serine/threonine kinases
Subcellular Localization
  • Nucleus
  • Chromosome, centromere, kinetochore
  • Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
  • Cytoplasm, cytoskeleton, spindle
  • Midbody
  • localization at the centrosome starts at the G1/S transition (By similarity)
  • During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores
  • Localizes to the outer kinetochore
  • Presence of SGO1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization
  • Localizes onto the central spindle by phosphorylating and docking at midzone proteins KIF20A/MKLP2 and PRC1 (By similarity)
  • Colocalizes with FRY to separating centrosomes and spindle poles from prophase to metaphase in mitosis, but not in other stages of the cell cycle (By similarity)
  • Localization to the centrosome is required for S phase progression (By similarity)
  • Colocalizes with HSF1 at the spindle poles during prometaphase (By similarity)
PANTHER Family PTHR24345 SERINE/THREONINE-PROTEIN KINASE PLK
PANTHER Subfamily PTHR24345:SF0 SERINE_THREONINE-PROTEIN KINASE PLK1
PANTHER Protein Class non-receptor serine/threonine protein kinase
protein modifying enzyme
PANTHER Pathway Category No pathway information available

18 GO annotations of cellular component

Name Definition
centriolar satellite A small (70-100 nm) cytoplasmic granule that contains a number of centrosomal proteins; centriolar satellites traffic toward microtubule minus ends and are enriched near the centrosome.
centriole A cellular organelle, found close to the nucleus in many eukaryotic cells, consisting of a small cylinder with microtubular walls, 300-500 nm long and 150-250 nm in diameter. It contains nine short, parallel, peripheral microtubular fibrils, each fibril consisting of one complete microtubule fused to two incomplete microtubules. Cells usually have two centrioles, lying at right angles to each other. At division, each pair of centrioles generates another pair and the twin pairs form the pole of the mitotic spindle.
centrosome A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
chromatin The ordered and organized complex of DNA, protein, and sometimes RNA, that forms the chromosome.
chromosome, centromeric region The region of a chromosome that includes the centromeric DNA and associated proteins. In monocentric chromosomes, this region corresponds to a single area of the chromosome, whereas in holocentric chromosomes, it is evenly distributed along the chromosome.
condensed chromosome, centromeric region The region of a condensed chromosome that includes the centromere and associated proteins, including the kinetochore. In monocentric chromosomes, this region corresponds to a single area of the chromosome, whereas in holocentric chromosomes, it is evenly distributed along the chromosome.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
kinetochore A multisubunit complex that is located at the centromeric region of DNA and provides an attachment point for the spindle microtubules.
microtubule cytoskeleton The part of the cytoskeleton (the internal framework of a cell) composed of microtubules and associated proteins.
midbody A thin cytoplasmic bridge formed between daughter cells at the end of cytokinesis. The midbody forms where the contractile ring constricts, and may persist for some time before finally breaking to complete cytokinesis.
mitotic spindle pole Either of the ends of a mitotic spindle, a spindle that forms as part of mitosis, where spindle microtubules are organized; usually contains a microtubule organizing center and accessory molecules, spindle microtubules and astral microtubules.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
outer kinetochore The region of a kinetochore most external to centromeric DNA; this outer region mediates kinetochore-microtubule interactions.
spindle The array of microtubules and associated molecules that forms between opposite poles of a eukaryotic cell during mitosis or meiosis and serves to move the duplicated chromosomes apart.
spindle microtubule Any microtubule that is part of a mitotic or meiotic spindle; anchored at one spindle pole.
spindle midzone The area in the center of the spindle where the spindle microtubules from opposite poles overlap.
spindle pole Either of the ends of a spindle, where spindle microtubules are organized; usually contains a microtubule organizing center and accessory molecules, spindle microtubules and astral microtubules.
synaptonemal complex A proteinaceous scaffold found between homologous chromosomes during meiosis. It consists of 2 lateral elements and a central element, all running parallel to each other. Transverse filaments connect the lateral elements to the central element.

10 GO annotations of molecular function

Name Definition
anaphase-promoting complex binding Binding to an anaphase-promoting complex. A ubiquitin ligase complex that degrades mitotic cyclins and anaphase inhibitory protein, thereby triggering sister chromatid separation and exit from mitosis.
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
identical protein binding Binding to an identical protein or proteins.
magnesium ion binding Binding to a magnesium (Mg) ion.
microtubule binding Binding to a microtubule, a filament composed of tubulin monomers.
protein kinase activity Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP.
protein kinase binding Binding to a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
protein serine/threonine/tyrosine kinase activity Catalysis of the reactions: ATP + a protein serine = ADP + protein serine phosphate; ATP + a protein threonine = ADP + protein threonine phosphate; and ATP + a protein tyrosine = ADP + protein tyrosine phosphate.

36 GO annotations of biological process

Name Definition
centrosome cycle The cell cycle process in which centrosome duplication and separation takes place. The centrosome cycle can operate with a considerable degree of independence from other processes of the cell cycle.
establishment of mitotic spindle orientation A cell cycle process that sets the alignment of mitotic spindle relative to other cellular structures.
establishment of protein localization The directed movement of a protein to a specific location.
female meiosis chromosome segregation The cell cycle process in which genetic material, in the form of chromosomes, is organized and then physically separated and apportioned to two or more sets during the meiotic cell cycle in a female.
G2/M transition of mitotic cell cycle The mitotic cell cycle transition by which a cell in G2 commits to M phase. The process begins when the kinase activity of M cyclin/CDK complex reaches a threshold high enough for the cell cycle to proceed. This is accomplished by activating a positive feedback loop that results in the accumulation of unphosphorylated and active M cyclin/CDK complex.
homologous chromosome segregation The cell cycle process in which replicated homologous chromosomes are organized and then physically separated and apportioned to two sets during the first division of the meiotic cell cycle. Each replicated chromosome, composed of two sister chromatids, aligns at the cell equator, paired with its homologous partner; this pairing off, referred to as synapsis, permits genetic recombination. One homolog (both sister chromatids) of each morphologic type goes into each of the resulting chromosome sets.
microtubule bundle formation A process that results in a parallel arrangement of microtubules.
mitotic cell cycle Progression through the phases of the mitotic cell cycle, the most common eukaryotic cell cycle, which canonically comprises four successive phases called G1, S, G2, and M and includes replication of the genome and the subsequent segregation of chromosomes into daughter cells. In some variant cell cycles nuclear replication or nuclear division may not be followed by cell division, or G1 and G2 phases may be absent.
mitotic cytokinesis A cell cycle process that results in the division of the cytoplasm of a cell after mitosis, resulting in the separation of the original cell into two daughter cells.
mitotic G2 DNA damage checkpoint signaling A mitotic cell cycle checkpoint that detects and negatively regulates progression through the G2/M transition of the cell cycle in response to DNA damage.
mitotic sister chromatid segregation The cell cycle process in which replicated homologous chromosomes are organized and then physically separated and apportioned to two sets during the mitotic cell cycle. Each replicated chromosome, composed of two sister chromatids, aligns at the cell equator, paired with its homologous partner. One homolog of each morphologic type goes into each of the resulting chromosome sets.
mitotic spindle assembly checkpoint signaling A signal transduction process that contributes to a mitotic cell cycle spindle assembly checkpoint, that delays the metaphase/anaphase transition of a mitotic nuclear division until the spindle is correctly assembled and chromosomes are attached to the spindle.
negative regulation of apoptotic process Any process that stops, prevents, or reduces the frequency, rate or extent of cell death by apoptotic process.
negative regulation of cyclin-dependent protein serine/threonine kinase activity Any process that stops, prevents, or reduces the frequency, rate or extent of cyclin-dependent protein serine/threonine kinase activity.
negative regulation of transcription by RNA polymerase II Any process that stops, prevents, or reduces the frequency, rate or extent of transcription mediated by RNA polymerase II.
nuclear membrane disassembly The controlled breakdown of the nuclear membranes, for example during cellular division.
peptidyl-serine phosphorylation The phosphorylation of peptidyl-serine to form peptidyl-O-phospho-L-serine.
polar body extrusion after meiotic divisions The cell cycle process in which two small cells are generated, as byproducts destined to degenerate, as a result of the first and second meiotic divisions of a primary oocyte during its development to a mature ovum. One polar body is formed in the first division of meiosis and the other in the second division; at each division, the cytoplasm divides unequally, so that the polar body is of much smaller size than the developing oocyte. At the second division in which a polar body is formed, the polar body and the developing oocyte each contain a haploid set of chromosomes.
positive regulation of peptidyl-threonine phosphorylation Any process that increases the frequency, rate or extent of peptidyl-threonine phosphorylation. Peptidyl-threonine phosphorylation is the phosphorylation of peptidyl-threonine to form peptidyl-O-phospho-L-threonine.
positive regulation of proteasomal ubiquitin-dependent protein catabolic process Any process that activates or increases the frequency, rate or extent of the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of ubiquitin, and mediated by the proteasome.
positive regulation of proteolysis Any process that activates or increases the frequency, rate or extent of the hydrolysis of a peptide bond or bonds within a protein.
positive regulation of ubiquitin protein ligase activity Any process that activates or increases the frequency, rate or extent of ubiquitin protein ligase activity.
positive regulation of ubiquitin-protein transferase activity Any process that activates, maintains or increases the rate of ubiquitin transferase activity.
protein destabilization Any process that decreases the stability of a protein, making it more vulnerable to degradative processes or aggregation.
protein localization to chromatin Any process in which a protein is transported to, or maintained at, a part of a chromosome that is organized into chromatin.
protein localization to nuclear envelope A process in which a protein is transported to, or maintained at, a location within a nuclear envelope.
protein localization to organelle A process in which a protein is transported to, or maintained in, a location within an organelle.
protein phosphorylation The process of introducing a phosphate group on to a protein.
protein ubiquitination The process in which one or more ubiquitin groups are added to a protein.
regulation of cytokinesis Any process that modulates the frequency, rate or extent of the division of the cytoplasm of a cell and its separation into two daughter cells.
regulation of mitotic cell cycle Any process that modulates the rate or extent of progress through the mitotic cell cycle.
regulation of mitotic metaphase/anaphase transition Any process that modulates the frequency, rate or extent of the cell cycle process in which a cell progresses from metaphase to anaphase during mitosis, triggered by the activation of the anaphase promoting complex by Cdc20/Sleepy homolog which results in the degradation of Securin.
regulation of mitotic spindle assembly Any process that modulates the frequency, rate or extent of mitotic spindle assembly.
regulation of protein binding Any process that modulates the frequency, rate or extent of protein binding.
regulation of protein localization to cell cortex Any process that modulates the frequency, rate or extent of protein localization to cell cortex.
synaptonemal complex disassembly The controlled breakdown of a synaptonemal complex.

9 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q2TA25 PLK1 Serine/threonine-protein kinase PLK1 Bos taurus (Bovine) SS
O97143 SAK Serine/threonine-protein kinase PLK4 Drosophila melanogaster (Fruit fly) EV
P52304 polo Serine/threonine-protein kinase polo Drosophila melanogaster (Fruit fly) SS
P53350 PLK1 Serine/threonine-protein kinase PLK1 Homo sapiens (Human) EV
Q62673 Plk1 Serine/threonine-protein kinase PLK1 Rattus norvegicus (Rat) SS
Q20845 plk-3 Serine/threonine-protein kinase plk-3 Caenorhabditis elegans PR
Q9N2L7 plk-2 Serine/threonine-protein kinase plk-2 Caenorhabditis elegans SS
P34331 plk-1 Serine/threonine-protein kinase plk-1 Caenorhabditis elegans SS
P62205 plk1 Serine/threonine-protein kinase PLK1 Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) SS
10 20 30 40 50 60
MNAAAKAGKL ARAPADLGKG GVPGDAVPGA PVAAPLAKEI PEVLVDPRSR RQYVRGRFLG
70 80 90 100 110 120
KGGFAKCFEI SDADTKEVFA GKIVPKSLLL KPHQKEKMSM EISIHRSLAH QHVVGFHDFF
130 140 150 160 170 180
EDSDFVFVVL ELCRRRSLLE LHKRRKALTE PEARYYLRQI VLGCQYLHRN QVIHRDLKLG
190 200 210 220 230 240
NLFLNEDLEV KIGDFGLATK VEYEGERKKT LCGTPNYIAP EVLSKKGHSF EVDVWSIGCI
250 260 270 280 290 300
MYTLLVGKPP FETSCLKETY LRIKKNEYSI PKHINPVAAS LIQKMLQTDP TARPTIHELL
310 320 330 340 350 360
NDEFFTSGYI PARLPITCLT IPPRFSIAPS SLDPSSRKPL KVLNKGVENP LPDRPREKEE
370 380 390 400 410 420
PVVRETNEAI ECHLSDLLQQ LTSVNASKPS ERGLVRQEEA EDPACIPIFW VSKWVDYSDK
430 440 450 460 470 480
YGLGYQLCDN SVGVLFNDST RLILYNDGDS LQYIERDGTE SYLTVSSHPN SLMKKITLLN
490 500 510 520 530 540
YFRNYMSEHL LKAGANITPR EGDELARLPY LRTWFRTRSA IILHLSNGTV QINFFQDHTK
550 560 570 580 590 600
LILCPLMAAV TYINEKRDFQ TYRLSLLEEY GCCKELASRL RYARTMVDKL LSSRSASNRL
KAS