Q07496
SS
Gene name |
EPHA4 (CEK8) |
Protein name |
Ephrin type-A receptor 4 |
Names |
EC 2.7.10.1 , EPH-like kinase 8 , EK8 , cEK8 |
Species |
Gallus gallus (Chicken) |
KEGG Pathway |
gga:395559 |
EC number |
2.7.10.1: Protein-tyrosine kinases |
Protein Class |
|
Descriptions
Ephrin type-B receptor 2 is a membrane-associated protein that mediates axon guidance, cell migration and morphogenesis. The Eph receptor tyrosine kinase family is regulated by autophosphorylation within the juxtamembrane region and the kinase activation segment. The structure, supported by mutagenesis data, reveals that the juxtamembrane segment adopts a helical conformation that distorts the small lobe of the kinase domain, and blocks the activation segment from attaining an activated conformation. Phosphorylation of the conserved juxtamembrane tyrosines would relieve this autoinhibition by disturbing the association of the juxtamembrane segment with the kinase domain, while liberating phosphotyrosine sites for binding SH2 domains of target proteins.
Autoinhibitory domains (AIDs)
Target domain |
621-882 (Protein kinase domain) |
Relief mechanism |
PTM |
Assay |
|
Accessory elements
763-789 (Activation loop from InterPro)
Target domain |
621-882 (Protein kinase domain) |
Relief mechanism |
|
Assay |
|
References
Autoinhibited structure
Activated structure
1 structures for Q07496
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q07496-F1 | Predicted | AlphaFoldDB |
No variants for Q07496
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for Q07496 | |||||
No associated diseases with Q07496
9 regional properties for Q07496
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Protein kinase domain | 33 - 301 | IPR000719-1 |
| domain | Protein kinase domain | 411 - 674 | IPR000719-2 |
| domain | AGC-kinase, C-terminal | 302 - 371 | IPR000961 |
| active_site | Serine/threonine-protein kinase, active site | 157 - 169 | IPR008271-1 |
| active_site | Serine/threonine-protein kinase, active site | 526 - 538 | IPR008271-2 |
| binding_site | Protein kinase, ATP binding site | 39 - 65 | IPR017441-1 |
| binding_site | Protein kinase, ATP binding site | 417 - 440 | IPR017441-2 |
| domain | Protein kinase, C-terminal | 322 - 361 | IPR017892 |
| domain | MSK2, N-terminal catalytic domain | 32 - 364 | IPR037714 |
Functions
| Description | ||
|---|---|---|
| EC Number | 2.7.10.1 | Protein-tyrosine kinases |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
4 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| dendrite | A neuron projection that has a short, tapering, morphology. Dendrites receive and integrate signals from other neurons or from sensory stimuli, and conduct nerve impulses towards the axon or the cell body. In most neurons, the impulse is conveyed from dendrites to axon via the cell body, but in some types of unipolar neuron, the impulse does not travel via the cell body. |
| early endosome membrane | The lipid bilayer surrounding an early endosome. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
5 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| DH domain binding | Binding to a DH (Dbl homology) domain of a protein. The DH domain contains three structurally conserved regions separated by more variable regions. It is composed of 11 alpha helices that are folded into a flattened, elongated alpha-helix bundle in which two of the three conserved regions, conserved region 1 (CR1) and conserved region 3 (CR3), are exposed near the centre of one surface. CR1 and CR3, together with a part of alpha-6 and the DH/PH (pleckstrin homology) junction site, constitute the Rho GTPase interacting pocket. |
| GPI-linked ephrin receptor activity | Combining with a GPI-anchored ephrin to initiate a change in cell activity. |
| protein kinase activity | Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction |
| transmembrane-ephrin receptor activity | Combining with a transmembrane ephrin to initiate a change in cell activity. |
10 GO annotations of biological process
| Name | Definition |
|---|---|
| axon guidance | The chemotaxis process that directs the migration of an axon growth cone to a specific target site in response to a combination of attractive and repulsive cues. |
| cell adhesion | The attachment of a cell, either to another cell or to an underlying substrate such as the extracellular matrix, via cell adhesion molecules. |
| ephrin receptor signaling pathway | The series of molecular signals initiated by ephrin binding to its receptor, and ending with the regulation of a downstream cellular process, e.g. transcription. |
| fasciculation of sensory neuron axon | The collection of sensory neuron axons into a bundle of rods, known as a fascicle. |
| peptidyl-tyrosine phosphorylation | The phosphorylation of peptidyl-tyrosine to form peptidyl-O4'-phospho-L-tyrosine. |
| positive regulation of Rho guanyl-nucleotide exchange factor activity | Any process that activates or increases the frequency, rate or extent of Rho guanyl-nucleotide exchange factor activity. |
| protein autophosphorylation | The phosphorylation by a protein of one or more of its own amino acid residues (cis-autophosphorylation), or residues on an identical protein (trans-autophosphorylation). |
| regulation of GTPase activity | Any process that modulates the rate of GTP hydrolysis by a GTPase. |
| segmentation | The regionalization process that divides an organism or part of an organism into a series of semi-repetitive parts, or segments, often arranged along a longitudinal axis. |
| somitogenesis | The formation of mesodermal clusters that are arranged segmentally along the anterior posterior axis of an embryo. |
52 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q07494 | EPHB1 | Ephrin type-B receptor 1 | Gallus gallus (Chicken) | SS |
| O42422 | EPHA7 | Ephrin type-A receptor 7 | Gallus gallus (Chicken) | SS |
| P29318 | EPHA3 | Ephrin type-A receptor 3 | Gallus gallus (Chicken) | SS |
| Q07497 | EPHB5 | Ephrin type-B receptor 5 | Gallus gallus (Chicken) | PR |
| P28693 | EPHB2 | Ephrin type-B receptor 2 | Gallus gallus (Chicken) | PR |
| P54755 | EPHA5 | Ephrin type-A receptor 5 | Gallus gallus (Chicken) | SS |
| Q07498 | EPHB3 | Ephrin type-B receptor 3 | Gallus gallus (Chicken) | SS |
| P0C0K6 | EPHB6 | Ephrin type-B receptor 6 | Pan troglodytes (Chimpanzee) | SS |
| P29322 | EPHA8 | Ephrin type-A receptor 8 | Homo sapiens (Human) | SS |
| P21709 | EPHA1 | Ephrin type-A receptor 1 | Homo sapiens (Human) | SS |
| P54753 | EPHB3 | Ephrin type-B receptor 3 | Homo sapiens (Human) | SS |
| P29320 | EPHA3 | Ephrin type-A receptor 3 | Homo sapiens (Human) | PR |
| P54760 | EPHB4 | Ephrin type-B receptor 4 | Homo sapiens (Human) | SS |
| P29317 | EPHA2 | Ephrin type-A receptor 2 | Homo sapiens (Human) | SS |
| Q15375 | EPHA7 | Ephrin type-A receptor 7 | Homo sapiens (Human) | SS |
| Q5JZY3 | EPHA10 | Ephrin type-A receptor 10 | Homo sapiens (Human) | SS |
| P29323 | EPHB2 | Ephrin type-B receptor 2 | Homo sapiens (Human) | EV |
| Q9UF33 | EPHA6 | Ephrin type-A receptor 6 | Homo sapiens (Human) | SS |
| P54762 | EPHB1 | Ephrin type-B receptor 1 | Homo sapiens (Human) | SS |
| P54756 | EPHA5 | Ephrin type-A receptor 5 | Homo sapiens (Human) | SS |
| O15197 | EPHB6 | Ephrin type-B receptor 6 | Homo sapiens (Human) | SS |
| P54764 | EPHA4 | Ephrin type-A receptor 4 | Homo sapiens (Human) | SS |
| P54754 | Ephb3 | Ephrin type-B receptor 3 | Mus musculus (Mouse) | SS |
| Q03145 | Epha2 | Ephrin type-A receptor 2 | Mus musculus (Mouse) | PR |
| Q61772 | Epha7 | Ephrin type-A receptor 7 | Mus musculus (Mouse) | SS |
| O09127 | Epha8 | Ephrin type-A receptor 8 | Mus musculus (Mouse) | SS |
| Q8CBF3 | Ephb1 | Ephrin type-B receptor 1 | Mus musculus (Mouse) | SS |
| Q8BYG9 | Epha10 | Ephrin type-A receptor 10 | Mus musculus (Mouse) | SS |
| Q60629 | Epha5 | Ephrin type-A receptor 5 | Mus musculus (Mouse) | SS |
| P29319 | Epha3 | Ephrin type-A receptor 3 | Mus musculus (Mouse) | SS |
| Q62413 | Epha6 | Ephrin type-A receptor 6 | Mus musculus (Mouse) | SS |
| Q60750 | Epha1 | Ephrin type-A receptor 1 | Mus musculus (Mouse) | SS |
| P54763 | Ephb2 | Ephrin type-B receptor 2 | Mus musculus (Mouse) | SS |
| P54761 | Ephb4 | Ephrin type-B receptor 4 | Mus musculus (Mouse) | PR |
| O08644 | Ephb6 | Ephrin type-B receptor 6 | Mus musculus (Mouse) | PR |
| Q03137 | Epha4 | Ephrin type-A receptor 4 | Mus musculus (Mouse) | SS |
| P54757 | Epha5 | Ephrin type-A receptor 5 | Rattus norvegicus (Rat) | SS |
| P54759 | Epha7 | Ephrin type-A receptor 7 | Rattus norvegicus (Rat) | SS |
| P09759 | Ephb1 | Ephrin type-B receptor 1 | Rattus norvegicus (Rat) | SS |
| P0C0K7 | Ephb6 | Ephrin type-B receptor 6 | Rattus norvegicus (Rat) | SS |
| O08680 | Epha3 | Ephrin type-A receptor 3 | Rattus norvegicus (Rat) | SS |
| O61460 | vab-1 | Ephrin receptor 1 | Caenorhabditis elegans | SS |
| Q7X8C5 | WAKL2 | Wall-associated receptor kinase-like 2 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9S9M2 | WAKL4 | Wall-associated receptor kinase-like 4 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9LMN7 | WAK5 | Wall-associated receptor kinase 5 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9C9L5 | WAKL9 | Wall-associated receptor kinase-like 9 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q8VYA3 | WAKL10 | Wall-associated receptor kinase-like 10 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9M092 | WAKL17 | Wall-associated receptor kinase-like 17 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q0WNY5 | WAKL18 | Wall-associated receptor kinase-like 18 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| O13147 | ephb3 | Ephrin type-B receptor 3 | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| O73878 | ephb4b | Ephrin type-B receptor 4b | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| O13146 | epha3 | Ephrin type-A receptor 3 | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MAGVPVGALL | PLLVGVCGAV | TGSRVYPANE | VTLLDSRSVQ | GELGWIASPL | EGGWEEVSIM |
| 70 | 80 | 90 | 100 | 110 | 120 |
| DEKNTPIRTY | QVCNVMEPSQ | NNWLRTDWIP | REGAQRVYIE | IKFTLRDCNS | LPGVMGTCKE |
| 130 | 140 | 150 | 160 | 170 | 180 |
| TFNLYYYESN | NDKERFIRES | QFAKIDTIAA | DESFTQVDIG | DRIMKLNTEV | RDVGPLSKKG |
| 190 | 200 | 210 | 220 | 230 | 240 |
| FYLAFQDVGA | CIALVSVRVF | YKKCPLTVRN | LAQFPDTITG | ADTSSLVEVR | GSCVNNSEEK |
| 250 | 260 | 270 | 280 | 290 | 300 |
| DVPKMYCGAD | GEWLVPIGNC | LCNAGYEERN | GECQACKIGY | YKALSTDVAC | AKCPPHSYSI |
| 310 | 320 | 330 | 340 | 350 | 360 |
| WEGSTSCTCD | RGFFRAENDA | ASMPCTRPPS | APQNLISNVN | ETSVNLEWSA | PQNKGGRDDI |
| 370 | 380 | 390 | 400 | 410 | 420 |
| SYNVVCKRCG | AGEPSHCRSC | GSGVHFSPQQ | NGLKTTKVSI | TDLLAHTNYT | FEVWAVNGVS |
| 430 | 440 | 450 | 460 | 470 | 480 |
| KHNPSQDQAV | SVTVTTNQAA | PSPIALIQAK | EITRHSVALA | WLEPDRPNGV | ILEYEVKYYE |
| 490 | 500 | 510 | 520 | 530 | 540 |
| KDQNERSYRI | VKTASRNTDI | KGLNPLTSYV | FHVRARTAAG | YGDFSGPFEF | TTNTVPSPII |
| 550 | 560 | 570 | 580 | 590 | 600 |
| GDGTNPTVLL | VSVAGSVVLV | VILIAAFVIS | RRRSKYSKAK | QEADEEKHLN | QGVRTYVDPF |
| 610 | 620 | 630 | 640 | 650 | 660 |
| TYEDPNQAVR | EFAKEIDASC | IKIEKVIGVG | EFGEVCSGRL | KVPGKREICV | AIKTLKAGYT |
| 670 | 680 | 690 | 700 | 710 | 720 |
| DKQRRDFLSE | ASIMGQFDHP | NIIHLEGVVT | KCKPVMIITE | YMENGSLDAF | LRKNDGRFTV |
| 730 | 740 | 750 | 760 | 770 | 780 |
| IQLVGMLRGI | GSGMKYLSDM | SYVHRDLAAR | NILVNSNLVC | KVSDFGMSRV | LEDDPEAAYT |
| 790 | 800 | 810 | 820 | 830 | 840 |
| TRGGKIPIRW | TAPEAIAYRK | FTSASDVWSY | GIVMWEVMSY | GERPYWDMSN | QDVIKAIEEG |
| 850 | 860 | 870 | 880 | 890 | 900 |
| YRLPPPMDCP | IALHQLMLDC | WQKERSDRPK | FGQIVNMLDK | LIRNPNSLKR | TGSESSRPST |
| 910 | 920 | 930 | 940 | 950 | 960 |
| ALLDPSSPEF | SAVVSVSDWL | QAIKMERYKD | NFTAAGYTTL | EAVVHMNQDD | LARIGITAIT |
| 970 | 980 | ||||
| HQNKILSSVQ | AMRSQMQQMH | GRMVPV |