Q06263

EV
Gene name
VTA1 (YLR181C)
Protein name
Vacuolar protein sorting-associated protein VTA1
Names
VPS20-associated protein 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YLR181C
EC number
Protein Class
VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VTA1 HOMOLOG (PTHR46009)

Descriptions

Vta is a cofactor of AAA-ATPase Vps4 via Vps4 stimulatory element (VSE), which is required to functionalize the endosomal sorting complexes required for transport (ESCRTs). The VSE domain is autoinhibited by the Vta1 linker region (183-266), inhibiting the Vps4 stimulation. ESCRT-III binding to the Vta1 microtubule interacting and trafficking domains relieves linker region autoinhibition of the VSE to produce maximal activation of Vps4 during ESCRT function. Deletion of amino-terminal portions of the linker region results in a hyperactive form of Vta1, which stimulates Vps4 function by facilitating oligomerization of Vps4.

Autoinhibitory domains (AIDs)

183-266 (Portion of linker region) EV

Target domain

267-289 (VSE domain)

Relief mechanism

Partner binding

Assay

Deletion assay, Mutagenesis experiment

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

10 structures for Q06263

Entry ID Method Resolution Chain Position Source
2LUH NMR - A 1-167 PDB
2RKK X-ray 290 A A/B 1-167 PDB
2RKL X-ray 150 A A/B/C/D/E/F 280-330 PDB
3MHV X-ray 310 A A 289-330 PDB
5H7P NMR - A 1-167 PDB
5UIE EM 570 A H/I/J/K/L/M/N/O/P/Q/R/S 1-330 PDB
5XMK EM 418 A G/H/I/J/K/L/M/N 1-330 PDB
6AP1 EM 320 A H/I/J/K/L/M/N/O/P/Q/R/S 1-330 PDB
6OO2 EM 440 A H/I/J/K/L/M/N/O/P/Q/R/S 280-330 PDB
AF-Q06263-F1 Predicted AlphaFoldDB

8 variants for Q06263

Variant ID(s) Position Change Description Diseaes Association Provenance
s12-517581 31 A>V No SGRP
s12-517450 75 S>G No SGRP
s12-517345 110 D>N No SGRP
s12-517116 186 I>T No SGRP
s12-517096 193 H>Y No SGRP
s12-517009 222 D>N No SGRP
s12-516898 259 E>K No SGRP
s12-516847 276 H>N No SGRP

No associated diseases with Q06263

2 regional properties for Q06263

Type Name Position InterPro Accession
domain Vta1/callose synthase, N-terminal 6 - 165 IPR039431
domain Vta1, C-terminal 293 - 328 IPR041212

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm
  • Endosome membrane ; Peripheral membrane protein
PANTHER Family PTHR46009 VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VTA1 HOMOLOG
PANTHER Subfamily PTHR46009:SF1 VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VTA1 HOMOLOG
PANTHER Protein Class membrane traffic protein
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
endosome A vacuole to which materials ingested by endocytosis are delivered.
ESCRT IV complex An ESCRT complex that has AAA-ATPase activity and is involved in ESCRT-mediated intralumenal vesicle formation and the final stages of cytokinesis. The complex catalyzes disassembly of the ESCRT III filament around the neck of the budding vesicle in an ATP-driven reaction, resulting in membrane scission and recycling of the ESCRT III components back to the cytosol. In yeast, it is formed by the AAA ATPase Vps4 and its cofactor Vta1.
multivesicular body A type of endosome in which regions of the limiting endosomal membrane invaginate to form internal vesicles; membrane proteins that enter the internal vesicles are sequestered from the cytoplasm.

2 GO annotations of molecular function

Name Definition
ATPase activator activity Binds to and increases the activity of an ATP hydrolysis activity.
protein-macromolecule adaptor activity The binding activity of a protein that brings together two or more macromolecules in contact, permitting those molecules to function in a coordinated way. The adaptor can bring together two proteins, or a protein and another macromolecule such as a lipid or a nucleic acid.

4 GO annotations of biological process

Name Definition
late endosome to vacuole transport The directed movement of substances from late endosomes to the vacuole. In yeast, after transport to the prevacuolar compartment, endocytic content is delivered to the late endosome and on to the vacuole. This pathway is analogous to endosome to lysosome transport.
late endosome to vacuole transport via multivesicular body sorting pathway The directed movement of substances from endosomes to vacuoles by a pathway in which molecules are sorted into multivesicular bodies, which then fuse with the vacuole.
lipid transport The directed movement of lipids into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. Lipids are compounds soluble in an organic solvent but not, or sparingly, in an aqueous solvent.
protein transport The directed movement of proteins into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MASNAARVVA TAKDFDKVGL GIIGYYLQLY AVELILSEED RSQEMTALAT ELLDTIEAFK
70 80 90 100 110 120
KEIGGESEAE DSDKSLHVMN TLIHDQEKAK IYMLNFTMSL YNEKLKQLKD GPWDVMLKRS
130 140 150 160 170 180
LWCCIDLFSC ILHLWKENIS ETSTNSLQKR IKYCKIYLSK LAKGEIGSSD EKTLDYADFA
190 200 210 220 230 240
DDSEEIKDED VDHQTSDLEN NNNDKVEGLA PKDQTTSYEP VDEVPEFIDD ADSVNEEEQT
250 260 270 280 290 300
VDKNEDAITK DEQQVVKKEV DLTRPSAPSE PAAAEHKSYT KDELTKIMDR ASKIEQIQKL
310 320
AKYAISALNY EDLPTAKDEL TKALDLLNSI