AiPD: Autoinhibited Protein Database

Q03113

Gene name	GNA12
Protein name	Guanine nucleotide-binding protein subunit alpha-12
Names	G alpha-12 , G-protein subunit alpha-12
Species	Homo sapiens (Human)
KEGG Pathway	hsa:2768
EC number
Protein Class

Descriptions

Gαi1 is a G-protein alpha subunit that plays a crucial role in signal transduction pathway initiated by G-protein-coupled receptor (GPCR) activation. G proteins function as transducers downstream of GPCRs in numerous signaling cascades. The alpha subunit contains the guanine nucleotide binding site and alternates between an active (GTP-bound state) and an inactive (GDP-bound state). Activated GPCR induces conformational change in Gαi1, which allows for GDP-to-GTP exchange by binding to the GPCR-binding site within a Ras-like domain, leading to dissociation of the Gαi1 subunit and initiation of downstream signaling.

Autoinhibitory domains (AIDs)

86-204 (α-H domain) SS

Target domain	211-375 (Ras-like domain)
Relief mechanism	Ligand binding, Partner binding
Assay

AID

86-204 (α-H domain)

Target domain

211-375 (Ras-like domain)

Relief mechanism

Ligand binding (GTP binding), Partner binding(Binding of activated GPCR)

Assay

Accessory elements

No accessory elements

References

Goricanec D et al. (2016) "Conformational dynamics of a G-protein α subunit is tightly regulated by nucleotide binding", Proceedings of the National Academy of Sciences of the United States of America, 113, E3629-38

Coleman DE et al. (1999) "Structure of Gialpha1.GppNHp, autoinhibition in a galpha protein-substrate complex", The Journal of biological chemistry, 274, 16669-72

Lutz S et al. (2007) "Structure of Galphaq-p63RhoGEF-RhoA complex reveals a pathway for the activation of RhoA by GPCRs", Science (New York, N.Y.), 318, 1923-7

Autoinhibited structure

Activated structure

2 structures for Q03113

Entry ID	Method	Resolution	Chain	Position	Source
7YDJ	EM	303 A			PDB
AF-Q03113-F1	Predicted				AlphaFoldDB

359 variants for Q03113

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
rs1779097221	2	S>Y		No	TOPMed
rs2114987464	3	G>E		No	Ensembl
rs1779096942	4	V>M		No	gnomAD
rs1260230928	7	T>S		No	gnomAD
rs1472157324	7	T>S		No	TOPMed gnomAD
rs1779096296	9	S>G		No	Ensembl
rs1265709948	9	S>I		No	TOPMed gnomAD
rs1779096013	10	R>C		No	TOPMed gnomAD
rs2114987432	10	R>L		No	Ensembl
rs1200233467	12	L>M		No	TOPMed
rs2114987426	12	L>R		No	Ensembl
TCGA novel	14	P>L	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA
rs1476538889	14	P>R		No	TOPMed gnomAD
rs1263167985	14	P>S		No	TOPMed gnomAD
rs1583324621	16	E>D		No	TOPMed
rs2114987414	16	E>K		No	Ensembl
rs1366360746	17	A>V		No	TOPMed gnomAD
rs1779094893	19	G>E		No	Ensembl
rs1161173743	19	G>R		No	TOPMed
rs1161173743	19	G>W		No	TOPMed
rs2114987398	20	A>G		No	Ensembl
rs1439912897	20	A>S		No	gnomAD
rs2114987394	22	E>*		No	Ensembl
rs1779094535	23	R>H		No	Ensembl
rs1779094348	26	G>D		No	Ensembl
rs2114987379	26	G>S		No	Ensembl
rs1779094180	28	G>C		No	TOPMed
rs1196500408	29	A>T		No	gnomAD
rs1469782889	31	D>N		No	TOPMed gnomAD
rs1562455202	32	A>T		No	Ensembl
rs947233017	33	E>G		No	gnomAD
rs1180111592	34	R>C		No	TOPMed gnomAD
rs1358887041	35	E>*		No	Ensembl
rs1290452555	35	E>G		No	gnomAD
rs991432234	37	R>G		No	TOPMed gnomAD
rs991432234	37	R>W		No	TOPMed gnomAD
rs1195142459	42	D>H		No	gnomAD
rs1195142459	42	D>N		No	gnomAD
rs1779092620	43	I>V		No	TOPMed
rs1779092373	45	A>S		No	TOPMed
rs775373263	45	A>V		No	ExAC TOPMed gnomAD
rs769505548	46	L>V		No	ExAC TOPMed gnomAD
rs1779092062	48	A>V		No	Ensembl
rs1310334094	49	R>C		No	gnomAD
rs1779091841	49	R>H		No	TOPMed
rs1310334094	49	R>S		No	gnomAD
rs1242547838	50	E>D		No	gnomAD
rs757276606	51	R>L		No	ExAC gnomAD
rs757276606	51	R>Q		No	ExAC gnomAD
rs1374831388	51	R>W		No	gnomAD
rs1396572923	52	R>C		No	gnomAD
rs1465410868	54	V>A		No	gnomAD
rs1300879649	54	V>F		No	gnomAD
rs1417455995	55	R>L	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA TOPMed gnomAD
rs1417455995	55	R>Q		No	TOPMed gnomAD
rs778979465	55	R>W		No	ExAC TOPMed gnomAD
rs1421445311	56	R>C		No	TOPMed gnomAD
rs1300066969	56	R>H		No	TOPMed
rs374498518	57	L>P		No	ESP ExAC gnomAD
rs766629746	58	V>M		No	ExAC gnomAD
rs756414986	59	K>R		No	ExAC gnomAD
rs756414986	59	K>T		No	ExAC gnomAD
rs768279667	60	I>M		No	gnomAD
rs762390854	63	L>Q		No	ExAC gnomAD
rs1779090205	64	G>R		No	Ensembl
rs866666086	66	G>D		No	Ensembl
rs1444967207	67	E>*		No	gnomAD
VAR_071044 rs11552939	68	S>G		No	UniProt dbSNP gnomAD
rs1309006560	69	G>S		No	TOPMed gnomAD
rs1395349919	72	T>K		No	gnomAD
rs1299756954	74	L>F		No	gnomAD
rs1583324411	78	R>S		No	Ensembl
COSM4834638	80	I>M	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA Cosmic
rs769533838	81	H>Y		No	ExAC gnomAD
rs1287424694	82	G>D		No	TOPMed
rs776555586	82	G>R		No	ExAC gnomAD
rs776555586	82	G>S		No	ExAC gnomAD
rs770705735	83	R>C		No	ExAC TOPMed gnomAD
rs1779088555	83	R>H		No	TOPMed
rs770705735	83	R>S		No	ExAC TOPMed gnomAD
rs1324713245	85	F>L		No	TOPMed gnomAD
rs1005951200	86	D>H		No	TOPMed gnomAD
rs1257817344	87	Q>H		No	TOPMed gnomAD
rs1404261944	88	K>Q	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA gnomAD
rs2114987139	88	K>T		No	Ensembl
rs1177705019	89	A>E		No	TOPMed gnomAD
rs2114987135	89	A>S		No	Ensembl
rs1177705019	89	A>V		No	TOPMed gnomAD
rs778926459	90	L>M		No	ExAC gnomAD
rs1779087799	91	L>Q		No	Ensembl
rs755021382	91	L>V		No	ExAC TOPMed gnomAD
rs1358616075	94	R>H	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA gnomAD
rs1358616075	94	R>L		No	gnomAD
rs2114987114	95	D>N		No	Ensembl
rs375882198	96	T>I		No	ESP TOPMed gnomAD
rs375882198	96	T>S		No	ESP TOPMed gnomAD
rs997016632	97	I>V		No	gnomAD
rs148809988	98	F>L		No	ESP ExAC TOPMed gnomAD
rs781627594	99	D>A		No	ExAC TOPMed gnomAD
rs1583324321	99	D>E		No	TOPMed
rs781627594	99	D>G		No	ExAC TOPMed gnomAD
rs1779087124	99	D>H		No	Ensembl
rs757785133	100	N>S		No	ExAC gnomAD
rs1779086824	101	I>V		No	TOPMed gnomAD
rs1292399463	102	L>F		No	gnomAD
rs1310625909	103	K>E		No	TOPMed
rs372701112	104	G>C		No	ESP ExAC TOPMed gnomAD
rs1792626538	105	S>L		No	Ensembl
rs780830669	106	R>S		No	TOPMed gnomAD
rs1562432727	108	L>F		No	TOPMed
rs1562432727	108	L>I		No	TOPMed
rs777583728	109	V>A		No	ExAC gnomAD
COSM6027342	112	R>*	Variant assessed as Somatic; HIGH impact. [NCI-TCGA]	No	NCI-TCGA Cosmic
rs754971607	115	L>F		No	ExAC TOPMed gnomAD
rs1047457263	116	G>S		No	TOPMed gnomAD
rs145512130	121	Y>H		No	ESP ExAC TOPMed gnomAD
rs1291227305	123	E>K		No	gnomAD
rs1792624703	125	E>K		No	TOPMed
rs1792624640	126	K>T		No	TOPMed gnomAD
rs751704187	127	H>N		No	ExAC gnomAD
rs764422187	127	H>Q		No	ExAC gnomAD
rs751704187	127	H>Y		No	ExAC gnomAD
rs894208409	128	G>W		No	Ensembl
rs1301833404	131	L>M		No	TOPMed gnomAD
rs1301833404	131	L>V		No	TOPMed gnomAD
rs746845598	134	F>L		No	ExAC TOPMed gnomAD
rs775896855	134	F>V		No	ExAC TOPMed gnomAD
COSM5552816 rs190617612	135	E>K	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA Cosmic 1000Genomes ExAC NCI-TCGA TOPMed gnomAD
rs1244997823	136	N>H		No	TOPMed
rs879083916	137	K>N		No	Ensembl
rs1181416866	138	A>V		No	TOPMed
rs746569487	139	G>A		No	ExAC gnomAD
rs1163400593	139	G>W		No	gnomAD
rs12721527	140	L>P		No	Ensembl
rs1792623038	141	P>H		No	TOPMed
TCGA novel	141	P>L	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA
rs1195630460 COSM3637777	141	P>S	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA Cosmic NCI-TCGA gnomAD
rs1331790514	142	V>A		No	gnomAD
rs140101989	142	V>L		No	ESP ExAC TOPMed gnomAD
rs1210452556	143	E>Q		No	gnomAD
rs778866783	144	P>L		No	ExAC TOPMed gnomAD
rs1212952961	145	A>T		No	gnomAD
rs751715632	151	V>F		No	ExAC TOPMed gnomAD
rs751715632	151	V>I		No	ExAC TOPMed gnomAD
rs751715632	151	V>L		No	ExAC TOPMed gnomAD
rs752999465	152	P>A		No	ExAC TOPMed gnomAD
rs1792621771	152	P>L		No	TOPMed gnomAD
rs1324531539	153	A>T	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA gnomAD
rs759995518	155	S>N		No	ExAC TOPMed gnomAD
rs766968121	156	A>S		No	ExAC TOPMed gnomAD
rs766968121	156	A>T		No	ExAC TOPMed gnomAD
rs760099419	156	A>V		No	ExAC gnomAD
COSM1568449	159	R>G	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA Cosmic
rs1792620963	159	R>K		No	TOPMed
rs1792620816	161	S>A		No	TOPMed
COSM746284	163	I>F	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA Cosmic
rs1792620682	163	I>V		No	TOPMed
rs1256491972	164	R>K		No	TOPMed gnomAD
rs2115457458	164	R>S		No	Ensembl
TCGA novel	167	F>L	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA
rs747827635	168	S>G		No	ExAC gnomAD
rs1792620284	168	S>I		No	TOPMed
COSM1549694 rs774244411	169	R>Q	lung [Cosmic]	No	cosmic curated 1000Genomes ExAC TOPMed gnomAD
rs1041978667	169	R>W		No	TOPMed
rs1193799566	170	R>G		No	TOPMed gnomAD
rs749152313	171	S>G		No	ExAC gnomAD
rs142768832	171	S>N		No	ESP TOPMed gnomAD
rs764331463	175	L>P		No	ExAC gnomAD
rs1790005677	177	E>Q		No	TOPMed
rs1790005436	178	S>A		No	Ensembl
rs777097834	178	S>L		No	ExAC gnomAD
rs771393290	179	V>M		No	ExAC gnomAD
rs1169894748	183	L>P		No	gnomAD
rs1021817793	185	N>S		No	TOPMed
COSM297183	186	L>F	Variant assessed as Somatic; HIGH impact. [NCI-TCGA]	No	NCI-TCGA Cosmic
rs368804687	187	D>E		No	ESP ExAC TOPMed gnomAD
rs758894496	189	I>M		No	ExAC gnomAD
rs747636303	190	G>S		No	ExAC TOPMed gnomAD
rs1455629624	191	Q>H		No	gnomAD
rs1583205691	191	Q>P		No	Ensembl
rs764178346	193	N>H		No	ExAC gnomAD
rs759677261	195	F>L		No	ExAC gnomAD
rs1276285181	196	P>A		No	TOPMed gnomAD
rs1276285181	196	P>S		No	TOPMed gnomAD
rs754038385	197	S>G		No	ExAC TOPMed gnomAD
rs766643469	201	I>T		No	ExAC TOPMed gnomAD
rs1789907598	201	I>V		No	Ensembl
rs772291731	205	R>S		No	ExAC TOPMed gnomAD
rs1789906652	206	K>R		No	TOPMed
rs1789906532	207	A>T		No	Ensembl
TCGA novel	207	A>V	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA
rs1208353672	209	K>M		No	TOPMed
TCGA novel	212	V>A	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA
rs1789905663	216	F>L		No	TOPMed
rs201475988	217	V>I	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	ESP ExAC NCI-TCGA TOPMed gnomAD
rs1789904833	218	I>V		No	TOPMed gnomAD
rs1562390540	220	K>N		No	Ensembl
rs375880168	222	P>S		No	ESP ExAC gnomAD
rs769392159	225	M>L		No	ExAC gnomAD
rs769392159	225	M>V		No	ExAC gnomAD
rs1430809713	226	V>M		No	Ensembl
rs1368316981	227	D>N		No	TOPMed gnomAD
rs780940374	228	V>M		No	ExAC gnomAD
TCGA novel	229	G>C	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA
rs1294969195	230	G>S	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA TOPMed
rs777850035	232	R>Q		No	ExAC gnomAD
rs980878134	232	R>W	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA TOPMed gnomAD
rs1235372215	233	S>A		No	gnomAD
rs1377175911	233	S>C		No	gnomAD
TCGA novel	233	S>T	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA
rs1789902296	235	R>C		No	Ensembl
COSM74769 COSM4947282	235	R>H	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA Cosmic
rs371474667	237	K>N		No	ESP ExAC TOPMed gnomAD
rs1431626569	239	F>L		No	gnomAD
COSM3637769 COSM3637770	240	Q>*	Variant assessed as Somatic; HIGH impact. [NCI-TCGA]	No	NCI-TCGA Cosmic
COSM3663127 rs1328252664	240	Q>H	liver [Cosmic]	No	cosmic curated TOPMed gnomAD
rs1226702362	240	Q>R		No	TOPMed gnomAD
rs45606633 VAR_049359	242	F>L		No	UniProt Ensembl dbSNP
rs1789901026	243	D>G		No	gnomAD
rs756257195	243	D>H		No	ExAC TOPMed gnomAD
rs756257195	243	D>N		No	ExAC TOPMed gnomAD
rs1166750331	244	G>E		No	gnomAD
rs1242852315	244	G>R		No	TOPMed
rs1294157750	245	I>V		No	TOPMed
rs767837122	246	T>M		No	ExAC TOPMed gnomAD
rs1583202899	247	S>T		No	Ensembl
rs762077179	248	I>V		No	ExAC gnomAD
rs774700519	249	L>V		No	ExAC gnomAD
rs1261139023	250	F>L		No	TOPMed
rs566672345	251	M>I		No	1000Genomes ExAC gnomAD
rs535681947	251	M>L		No	1000Genomes ExAC gnomAD
rs1239815747	251	M>T		No	gnomAD
rs745435606	255	S>G		No	ExAC gnomAD
COSM3832597 COSM3832596	255	S>N	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA Cosmic
rs745435606	255	S>R		No	ExAC gnomAD
rs999835892	258	D>N		No	TOPMed gnomAD
rs1562390388	259	Q>E		No	Ensembl
rs770685700	259	Q>L		No	ExAC gnomAD
rs201962977	260	V>F		No	1000Genomes ExAC TOPMed gnomAD
rs201962977	260	V>I		No	1000Genomes ExAC TOPMed gnomAD
rs201962977	260	V>L		No	1000Genomes ExAC TOPMed gnomAD
COSM1648001 rs1371708745 COSM746289	262	M>L	lung Variant assessed as Somatic; MODERATE impact. [Cosmic, NCI-TCGA]	No	cosmic curated TOPMed gnomAD NCI-TCGA Cosmic
rs1371708745	262	M>V		No	TOPMed gnomAD
rs1434085965	264	D>A		No	gnomAD
TCGA novel	265	R>G	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA
rs954175741	266	R>C		No	Ensembl
COSM3698352 rs758369709 COSM3698353	266	R>H	Variant assessed as Somatic; MODERATE impact. large_intestine [NCI-TCGA, Cosmic]	No	NCI-TCGA Cosmic cosmic curated ExAC NCI-TCGA TOPMed gnomAD
rs758369709	266	R>L		No	ExAC TOPMed gnomAD
rs758369709	266	R>P		No	ExAC TOPMed gnomAD
rs1329158036	267	T>N		No	TOPMed gnomAD
rs746042429 COSM387723	268	N>S	lung [Cosmic]	No	cosmic curated gnomAD
rs904215612	269	R>Q		No	TOPMed gnomAD
COSM4948645 COSM1450209	269	R>W	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA Cosmic
rs779040599	271	V>G		No	ExAC gnomAD
rs756132903	272	E>G		No	ExAC gnomAD
rs767565658	273	S>A		No	ExAC
rs767565658	273	S>T		No	ExAC
rs1789893724	274	M>T		No	gnomAD
COSM1088908 COSM4871445	275	N>D	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA Cosmic
rs1562390324	275	N>H		No	TOPMed
rs1487722362	277	F>L		No	gnomAD
rs200282933	278	E>K		No	Ensembl
rs751868693	279	T>N		No	ExAC gnomAD
rs2115279689	279	T>P		No	Ensembl
rs932431919	280	I>V		No	TOPMed gnomAD
rs150519840	281	V>I	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	1000Genomes ESP ExAC NCI-TCGA TOPMed gnomAD
rs150519840	281	V>L		No	1000Genomes ESP ExAC TOPMed gnomAD
rs758966311	282	N>D		No	ExAC gnomAD
rs776211969	282	N>I		No	ExAC
rs1789891389	283	N>K		No	gnomAD
rs1789891499	283	N>S		No	TOPMed
COSM4870843 rs1789891247 COSM1088907	284	K>R	Variant assessed as Somatic; MODERATE impact. endometrium [NCI-TCGA, Cosmic]	No	NCI-TCGA Cosmic cosmic curated TOPMed
rs1789890886	286	F>V		No	gnomAD
rs191664263	287	F>Y		No	1000Genomes ExAC
rs1789890488	288	N>S		No	TOPMed
rs771969963	289	V>I	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	ExAC NCI-TCGA TOPMed gnomAD
rs199802606	290	S>P		No	1000Genomes
rs1789889974	291	I>V		No	TOPMed
COSM1088906 COSM4869958	292	I>S	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA Cosmic
rs999964514	295	L>V		No	TOPMed
rs1434842389	297	K>T		No	gnomAD
rs745875941	299	D>E		No	ExAC TOPMed gnomAD
rs1424918885	305	V>M		No	TOPMed gnomAD
rs1789888423	307	T>S		No	TOPMed
rs1789887964	308	V>A		No	Ensembl
rs368581021	308	V>M	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	ESP ExAC NCI-TCGA TOPMed gnomAD
rs778023039	309	S>N		No	ExAC TOPMed gnomAD
rs1789887596	311	K>R		No	TOPMed
rs76478938	312	K>N		No	ExAC gnomAD
TCGA novel	312	K>Q	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA
rs1789887131	315	P>A		No	Ensembl
rs765598901	315	P>L		No	ExAC TOPMed gnomAD
rs765598901	315	P>R		No	ExAC TOPMed gnomAD
COSM1088905 rs1789887131 COSM4866887	315	P>S	Variant assessed as Somatic; MODERATE impact. endometrium [NCI-TCGA, Cosmic]	No	NCI-TCGA Cosmic cosmic curated Ensembl
rs753239425	316	D>G		No	ExAC TOPMed gnomAD
rs1562390197	316	D>N		No	Ensembl
rs753239425	316	D>V		No	ExAC TOPMed gnomAD
rs141784313	320	D>N		No	1000Genomes ESP ExAC TOPMed gnomAD
rs141784313	320	D>Y		No	1000Genomes ESP ExAC TOPMed gnomAD
rs111264276	321	P>S		No	Ensembl
rs761630254	322	H>Q		No	ExAC gnomAD
rs1414975774	322	H>R		No	TOPMed gnomAD
rs774205451	323	R>K		No	ExAC
rs1562390153	323	R>S		No	Ensembl
rs768426522	324	L>M		No	ExAC TOPMed gnomAD
rs768426522	324	L>V		No	ExAC TOPMed gnomAD
rs545924345	327	V>I		No	1000Genomes ExAC TOPMed gnomAD
rs1789884280	328	Q>H		No	Ensembl
rs143607374	329	R>C		No	ESP ExAC TOPMed gnomAD
rs777971344	329	R>H		No	ExAC TOPMed gnomAD
rs758624210	330	Y>F		No	ExAC TOPMed gnomAD
rs45583847 VAR_049360	330	Y>H		No	UniProt dbSNP gnomAD
rs1789882516	332	V>F		No	Ensembl
rs1789882516	332	V>I		No	Ensembl
rs753001643	333	Q>R		No	ExAC gnomAD
rs755401911	336	D>N		No	ExAC TOPMed gnomAD
rs754346065	337	R>K		No	ExAC gnomAD
rs1267185998	338	K>N		No	gnomAD
rs1264110448	340	R>Q		No	gnomAD
rs766851973	340	R>W		No	ExAC gnomAD
rs1583202314	341	N>T		No	Ensembl
rs1336567198	342	R>C		No	gnomAD
COSM1450208 COSM4786003 rs140322696	342	R>H	Variant assessed as Somatic; MODERATE impact. large_intestine [NCI-TCGA, Cosmic]	No	NCI-TCGA Cosmic cosmic curated ESP ExAC NCI-TCGA TOPMed gnomAD
rs140322696	342	R>L		No	ESP ExAC TOPMed gnomAD
rs1394982808	343	S>G		No	gnomAD
rs111404280	343	S>R		No	1000Genomes ExAC gnomAD
rs957106905	344	K>E		No	Ensembl
rs201860352	345	P>S		No	1000Genomes ExAC TOPMed gnomAD
rs773974899	346	L>V		No	ExAC TOPMed gnomAD
rs1158817269	348	H>Q		No	gnomAD
rs1462284322	349	H>N		No	gnomAD
rs12721528	350	F>L		No	Ensembl
rs1583202221	351	T>P		No	Ensembl
rs1789878855	352	T>I		No	Ensembl
rs1190438211	353	A>D		No	gnomAD
rs1262468448	353	A>S		No	gnomAD
rs1262468448	353	A>T	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA gnomAD
rs560123937	355	D>N		No	ExAC TOPMed gnomAD
rs560123937	355	D>Y		No	ExAC TOPMed gnomAD
rs904184373	357	E>G		No	Ensembl
rs200756903 COSM1088904 COSM1698332	357	E>K	Variant assessed as Somatic; MODERATE impact. skin endometrium breast [NCI-TCGA, Cosmic]	No	NCI-TCGA Cosmic cosmic curated ExAC NCI-TCGA TOPMed gnomAD
rs372644647	358	N>K		No	1000Genomes ESP ExAC TOPMed gnomAD
COSM3880434 COSM3880435 rs749682380	359	V>I	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA Cosmic ExAC NCI-TCGA gnomAD
rs780640776	360	R>C		No	ExAC gnomAD
rs1274321284	363	F>L		No	gnomAD
rs1180278804	364	H>R		No	TOPMed gnomAD
rs1789872246	368	D>N		No	TOPMed
rs1393299603	369	T>A		No	gnomAD
rs749900591	369	T>N		No	ExAC TOPMed gnomAD
rs749900591	369	T>S		No	ExAC TOPMed gnomAD
rs756865434	370	I>N		No	ExAC gnomAD
rs767164073	370	I>V		No	ExAC gnomAD
rs762806711	373	E>D		No	ExAC TOPMed gnomAD
COSM4162127 rs1789871200	376	K>N	thyroid [Cosmic]	No	cosmic curated Ensembl
COSM1088903 COSM4868984	378	I>S	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA Cosmic
rs1197066546	378	I>V		No	TOPMed gnomAD
rs765754611	380	L>M		No	TOPMed gnomAD
rs1264957572	381	Q>H		No	gnomAD
COSM3832590 COSM3832591	382	Q>=	Variant assessed as Somatic; LOW impact. [NCI-TCGA]	No	NCI-TCGA Cosmic

No associated diseases with Q03113

3 regional properties for Q03113

Type	Name	Position	InterPro Accession
domain	W2 domain	720 - 904	IPR003307
domain	MIF4G-like, type 3	78 - 308	IPR003890
domain	Initiation factor eIF-4 gamma, MA3	543 - 666	IPR003891

Functions

		Description
EC Number
Subcellular Localization	Cell membrane ; Lipid-anchor Lateral cell membrane ; Lipid-anchor Cytoplasm	CDH1 enhances cell membrane localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

8 GO annotations of cellular component

Name	Definition
brush border membrane	The portion of the plasma membrane surrounding the brush border.
cytoplasm	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
focal adhesion	A cell-substrate junction that anchors the cell to the extracellular matrix and that forms a point of termination of actin filaments. In insects focal adhesion has also been referred to as hemi-adherens junction (HAJ).
heterotrimeric G-protein complex	Any of a family of heterotrimeric GTP-binding and hydrolyzing proteins; they belong to a superfamily of GTPases that includes monomeric proteins such as EF-Tu and RAS. Heterotrimeric G-proteins consist of three subunits; the alpha subunit contains the guanine nucleotide binding site and possesses GTPase activity; the beta and gamma subunits are tightly associated and function as a beta-gamma heterodimer; extrinsic plasma membrane proteins (cytoplasmic face) that function as a complex to transduce signals from G protein-coupled receptors to an effector protein.
lateral plasma membrane	The portion of the plasma membrane at the lateral side of the cell. In epithelial cells, lateral plasma membranes are on the sides of cells which lie at the interface of adjacent cells.
neuron projection	A prolongation or process extending from a nerve cell, e.g. an axon or dendrite.
neuronal cell body	The portion of a neuron that includes the nucleus, but excludes cell projections such as axons and dendrites.
plasma membrane	The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

7 GO annotations of molecular function

Name	Definition
D5 dopamine receptor binding	Binding to a D5 dopamine receptor.
G-protein beta/gamma-subunit complex binding	Binding to a complex of G-protein beta/gamma subunits.
GTP binding	Binding to GTP, guanosine triphosphate.
GTPase activity	Catalysis of the reaction
metal ion binding	Binding to a metal ion.
protein phosphatase 2A binding	Binding to protein phosphatase 2A.
protein phosphatase regulator activity	Binds to and modulates the activity of a protein phosphatase.

16 GO annotations of biological process

Name	Definition
adenylate cyclase-modulating G protein-coupled receptor signaling pathway	A G protein-coupled receptor signaling pathway in which the signal is transmitted via the activation or inhibition of adenylyl cyclase activity and a subsequent change in the intracellular concentration of cyclic AMP (cAMP).
blood coagulation	The sequential process in which the multiple coagulation factors of the blood interact, ultimately resulting in the formation of an insoluble fibrin clot; it may be divided into three stages
cell differentiation	The cellular developmental process in which a relatively unspecialized cell, e.g. embryonic or regenerative cell, acquires specialized structural and/or functional features that characterize a specific cell. Differentiation includes the processes involved in commitment of a cell to a specific fate and its subsequent development to the mature state.
embryonic digit morphogenesis	The process, occurring in the embryo, by which the anatomical structures of the digit are generated and organized. A digit is one of the terminal divisions of an appendage, such as a finger or toe.
G protein-coupled receptor signaling pathway	The series of molecular signals initiated by a ligand binding to its receptor, in which the activated receptor promotes the exchange of GDP for GTP on the alpha-subunit of an associated heterotrimeric G-protein complex. The GTP-bound activated alpha-G-protein then dissociates from the beta- and gamma-subunits to further transmit the signal within the cell. The pathway begins with receptor-ligand interaction, and ends with regulation of a downstream cellular process. The pathway can start from the plasma membrane, Golgi or nuclear membrane.
in utero embryonic development	The process whose specific outcome is the progression of the embryo in the uterus over time, from formation of the zygote in the oviduct, to birth. An example of this process is found in Mus musculus.
negative regulation of vascular associated smooth muscle cell migration	Any process that stops, prevents or reduces the frequency, rate or extent of vascular associated smooth muscle cell migration.
negative regulation of vascular associated smooth muscle cell proliferation	Any process that stops, prevents or reduces the frequency, rate or extent of vascular smooth muscle cell proliferation.
positive regulation of phosphoprotein phosphatase activity	Any process that activates or increases the activity of a phosphoprotein phosphatase.
regulation of blood pressure	Any process that modulates the force with which blood travels through the circulatory system. The process is controlled by a balance of processes that increase pressure and decrease pressure.
regulation of cell shape	Any process that modulates the surface configuration of a cell.
regulation of fibroblast migration	Any process that modulates the rate, frequency or extent of fibroblast cell migration. Fibroblast cell migration is accomplished by extension and retraction of a pseudopodium.
regulation of proteasomal ubiquitin-dependent protein catabolic process	Any process that modulates the frequency, rate or extent of the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of ubiquitin, and mediated by the proteasome.
regulation of TOR signaling	Any process that modulates the frequency, rate or extent of TOR signaling.
response to xenobiotic stimulus	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus from a xenobiotic, a compound foreign to the organim exposed to it. It may be synthesized by another organism (like ampicilin) or it can be a synthetic chemical.
Rho protein signal transduction	The series of molecular signals within the cell that are mediated by a member of the Rho family of proteins switching to a GTP-bound active state.

61 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
P63097	GNAI1	Guanine nucleotide-binding protein G	Bos taurus (Bovine)	SS
P0C7Q4	GNAT3	Guanine nucleotide-binding protein G	Bos taurus (Bovine)	SS
P04696	GNAT2	Guanine nucleotide-binding protein G	Bos taurus (Bovine)	SS
P04695	GNAT1	Guanine nucleotide-binding protein G	Bos taurus (Bovine)	SS
P08239	GNAO1	Guanine nucleotide-binding protein G	Bos taurus (Bovine)	SS
P50147	GNAI2	Guanine nucleotide-binding protein G	Gallus gallus (Chicken)	SS
P50146	GNAI1	Guanine nucleotide-binding protein G	Gallus gallus (Chicken)	SS
P20353	Galphai	G protein alpha i subunit	Drosophila melanogaster (Fruit fly)	SS
P16378	Galphao	G protein alpha o subunit	Drosophila melanogaster (Fruit fly)	SS
P25157	cta	Guanine nucleotide-binding protein subunit alpha homolog	Drosophila melanogaster (Fruit fly)	SS
Q14344	GNA13	Guanine nucleotide-binding protein subunit alpha-13	Homo sapiens (Human)	SS
P29992	GNA11	Guanine nucleotide-binding protein subunit alpha-11	Homo sapiens (Human)	SS
P30679	GNA15	Guanine nucleotide-binding protein subunit alpha-15	Homo sapiens (Human)	SS
O95837	GNA14	Guanine nucleotide-binding protein subunit alpha-14	Homo sapiens (Human)	SS
P50148	GNAQ	Guanine nucleotide-binding protein G	Homo sapiens (Human)	SS
P38405	GNAL	Guanine nucleotide-binding protein G	Homo sapiens (Human)	SS
A8MTJ3	GNAT3	Guanine nucleotide-binding protein G	Homo sapiens (Human)	SS
P19087	GNAT2	Guanine nucleotide-binding protein G	Homo sapiens (Human)	SS
P11488	GNAT1	Guanine nucleotide-binding protein G	Homo sapiens (Human)	SS
P04899	GNAI2	Guanine nucleotide-binding protein G	Homo sapiens (Human)	SS
P08754	GNAI3	Guanine nucleotide-binding protein G	Homo sapiens (Human)	SS
P19086	GNAZ	Guanine nucleotide-binding protein G	Homo sapiens (Human)	SS
P63096	GNAI1	Guanine nucleotide-binding protein G	Homo sapiens (Human)	EV
P09471	GNAO1	Guanine nucleotide-binding protein G	Homo sapiens (Human)	SS
Q9DC51	Gnai3	Guanine nucleotide-binding protein G	Mus musculus (Mouse)	SS
P20612	Gnat1	Guanine nucleotide-binding protein G	Mus musculus (Mouse)	SS
P27601	Gna13	Guanine nucleotide-binding protein subunit alpha-13	Mus musculus (Mouse)	SS
Q3V3I2	Gnat3	Guanine nucleotide-binding protein G	Mus musculus (Mouse)	SS
O70443	Gnaz	Guanine nucleotide-binding protein G	Mus musculus (Mouse)	SS
P50149	Gnat2	Guanine nucleotide-binding protein G	Mus musculus (Mouse)	SS
P08752	Gnai2	Guanine nucleotide-binding protein G	Mus musculus (Mouse)	SS
B2RSH2	Gnai1	Guanine nucleotide-binding protein G	Mus musculus (Mouse)	SS
P18872	Gnao1	Guanine nucleotide-binding protein G	Mus musculus (Mouse)	SS
P27600	Gna12	Guanine nucleotide-binding protein subunit alpha-12	Mus musculus (Mouse)	SS
P93564	GPA1	Guanine nucleotide-binding protein alpha-1 subunit	Solanum tuberosum (Potato)	SS
P19627	Gnaz	Guanine nucleotide-binding protein G	Rattus norvegicus (Rat)	SS
P29348	Gnat3	Guanine nucleotide-binding protein G	Rattus norvegicus (Rat)	SS
P04897	Gnai2	Guanine nucleotide-binding protein G	Rattus norvegicus (Rat)	SS
P59215	Gnao1	Guanine nucleotide-binding protein G	Rattus norvegicus (Rat)	SS
P08753	Gnai3	Guanine nucleotide-binding protein G	Rattus norvegicus (Rat)	SS
Q6Q7Y5	Gna13	Guanine nucleotide-binding protein subunit alpha-13	Rattus norvegicus (Rat)	SS
Q63210	Gna12	Guanine nucleotide-binding protein subunit alpha-12	Rattus norvegicus (Rat)	SS
Q0DJ33	GPA1	Guanine nucleotide-binding protein alpha-1 subunit	Oryza sativa subsp. japonica (Rice)	SS
O76584	gpa-11	Guanine nucleotide-binding protein alpha-11 subunit	Caenorhabditis elegans	SS
P51875	goa-1	Guanine nucleotide-binding protein G	Caenorhabditis elegans	SS
Q18434	odr-3	Guanine nucleotide-binding protein alpha-17 subunit	Caenorhabditis elegans	SS
Q9BIG4	gpa-10	Guanine nucleotide-binding protein alpha-10 subunit	Caenorhabditis elegans	PR
P28052	gpa-3	Guanine nucleotide-binding protein alpha-3 subunit	Caenorhabditis elegans	SS
Q9XTB2	gpa-13	Guanine nucleotide-binding protein alpha-13 subunit	Caenorhabditis elegans	SS
Q93743	gpa-6	Guanine nucleotide-binding protein alpha-6 subunit	Caenorhabditis elegans	SS
Q20907	gpa-8	Guanine nucleotide-binding protein alpha-8 subunit	Caenorhabditis elegans	PR
P91907	gpa-15	Guanine nucleotide-binding protein alpha-15 subunit	Caenorhabditis elegans	SS
Q21917	gpa-7	Guanine nucleotide-binding protein alpha-7 subunit	Caenorhabditis elegans	SS
Q9BIG5	gpa-4	Guanine nucleotide-binding protein alpha-4 subunit	Caenorhabditis elegans	SS
P28051	gpa-1	Guanine nucleotide-binding protein alpha-1 subunit	Caenorhabditis elegans	SS
Q9N2V6	gpa-16	Guanine nucleotide-binding protein alpha-16 subunit	Caenorhabditis elegans	SS
P93163	GPA2	Guanine nucleotide-binding protein alpha-2 subunit	Glycine max (Soybean) (Glycine hispida)	SS
P49084	GPA1	Guanine nucleotide-binding protein alpha-1 subunit	Glycine max (Soybean) (Glycine hispida)	SS
O80462	XLG1	Extra-large guanine nucleotide-binding protein 1	Arabidopsis thaliana (Mouse-ear cress)	PR
P18064	GPA1	Guanine nucleotide-binding protein alpha-1 subunit	Arabidopsis thaliana (Mouse-ear cress)	SS
P26981	GPA1	Guanine nucleotide-binding protein alpha-1 subunit	Solanum lycopersicum (Tomato) (Lycopersicon esculentum)	SS

10	20	30	40	50	60
MSGVVRTLSR	CLLPAEAGGA	RERRAGSGAR	DAEREARRRS	RDIDALLARE	RRAVRRLVKI
70	80	90	100	110	120
LLLGAGESGK	STFLKQMRII	HGREFDQKAL	LEFRDTIFDN	ILKGSRVLVD	ARDKLGIPWQ
130	140	150	160	170	180
YSENEKHGMF	LMAFENKAGL	PVEPATFQLY	VPALSALWRD	SGIREAFSRR	SEFQLGESVK
190	200	210	220	230	240
YFLDNLDRIG	QLNYFPSKQD	ILLARKATKG	IVEHDFVIKK	IPFKMVDVGG	QRSQRQKWFQ
250	260	270	280	290	300
CFDGITSILF	MVSSSEYDQV	LMEDRRTNRL	VESMNIFETI	VNNKLFFNVS	IILFLNKMDL
310	320	330	340	350	360
LVEKVKTVSI	KKHFPDFRGD	PHRLEDVQRY	LVQCFDRKRR	NRSKPLFHHF	TTAIDTENVR
370	380
FVFHAVKDTI	LQENLKDIML	Q