Q02821
SS
Gene name |
SRP1 (KAP60, YNL189W, N1606) |
Protein name |
Importin subunit alpha |
Names |
Karyopherin subunit alpha , Karyopherin-60 , Serine-rich RNA polymerase I suppressor protein |
Species |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
KEGG Pathway |
sce:YNL189W |
EC number |
|
Protein Class |
|
Descriptions
Importin-α is a nuclear import receptor that facilitates the transport of proteins with nuclear localization sequences (NLSs) into the nucleus. It is autoinhibited by its N-terminal region, which occupies the NLS-binding site, preventing unnecessary binding and import activity. Importin-α is autoinhibited in the absence of importin-β and the binding of importin-β alleviates this autoinhibition, which displaces the autoinhibitory sequence.
Autoinhibitory domains (AIDs)
Target domain |
153-244 (Major NLS-binding site);321-409 (Minor NLS-binding site) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
No accessory elements
References
- Fontes MR et al. (2000) "Structural basis of recognition of monopartite and bipartite nuclear localization sequences by mammalian importin-alpha", Journal of molecular biology, 297, 1183-94
- Fontes MR et al. (2003) "Structural basis for the specificity of bipartite nuclear localization sequence binding by importin-alpha", The Journal of biological chemistry, 278, 27981-7
- Catimel B et al. (2001) "Biophysical characterization of interactions involving importin-alpha during nuclear import", The Journal of biological chemistry, 276, 34189-98
- Pumroy RA et al. (2015) "Molecular determinants for nuclear import of influenza A PB2 by importin α isoforms 3 and 7", Structure (London, England : 1993), 23, 374-84
Autoinhibited structure
Activated structure
13 structures for Q02821
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 1BK5 | X-ray | 220 A | A/B | 89-510 | PDB |
| 1BK6 | X-ray | 280 A | A/B | 89-510 | PDB |
| 1EE4 | X-ray | 210 A | A/B | 87-509 | PDB |
| 1EE5 | X-ray | 240 A | A | 87-510 | PDB |
| 1UN0 | X-ray | 260 A | A/B | 88-530 | PDB |
| 1WA5 | X-ray | 200 A | B | 1-530 | PDB |
| 2C1T | X-ray | 260 A | A/B | 88-541 | PDB |
| 4PVZ | X-ray | 250 A | A/B | 88-509 | PDB |
| 4XZR | X-ray | 225 A | B | 88-509 | PDB |
| 5H2W | X-ray | 250 A | A/C | 88-510 | PDB |
| 5H2X | X-ray | 220 A | A | 88-510 | PDB |
| 5T94 | X-ray | 263 A | B | 1-542 | PDB |
| AF-Q02821-F1 | Predicted | AlphaFoldDB |
No variants for Q02821
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for Q02821 | |||||
No associated diseases with Q02821
8 regional properties for Q02821
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| repeat | Armadillo | 119 - 202 | IPR000225-1 |
| repeat | Armadillo | 204 - 244 | IPR000225-2 |
| repeat | Armadillo | 247 - 286 | IPR000225-3 |
| repeat | Armadillo | 288 - 328 | IPR000225-4 |
| repeat | Armadillo | 330 - 412 | IPR000225-5 |
| repeat | Armadillo | 417 - 457 | IPR000225-6 |
| domain | Importin-alpha, importin-beta-binding domain | 1 - 108 | IPR002652 |
| repeat | Atypical Arm repeat | 471 - 523 | IPR032413 |
8 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| NLS-dependent protein nuclear import complex | A dimer consisting of an alpha and a beta-subunit that imports proteins with an NLS into the nucleus through a nuclear pore. |
| nuclear envelope | The double lipid bilayer enclosing the nucleus and separating its contents from the rest of the cytoplasm; includes the intermembrane space, a gap of width 20-40 nm (also called the perinuclear space). |
| nucleoplasm | That part of the nuclear content other than the chromosomes or the nucleolus. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| perinuclear region of cytoplasm | Cytoplasm situated near, or occurring around, the nucleus. |
| protein-containing complex | A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together. |
4 GO annotations of molecular function
| Name | Definition |
|---|---|
| disordered domain specific binding | Binding to a disordered domain of a protein. |
| nuclear import signal receptor activity | Combining with a nuclear import signal (NIS) on a cargo to be transported, to mediate transport of the cargo through the nuclear pore, from the cytoplasm to the nuclear lumen. The cargo can be either a RNA or a protein. |
| nuclear localization sequence binding | Binding to a nuclear localization sequence, a specific peptide sequence that acts as a signal to localize the protein within the nucleus. |
| protein-containing complex binding | Binding to a macromolecular complex. |
5 GO annotations of biological process
| Name | Definition |
|---|---|
| import into nucleus | The directed movement of substances into the nucleus. |
| NLS-bearing protein import into nucleus | The directed movement of a protein bearing a nuclear localization signal (NLS) from the cytoplasm into the nucleus, across the nuclear envelope. |
| proteasome localization | Any process in which the proteasome is transported to, or maintained in, a specific location. |
| protein import into nucleus | The directed movement of a protein from the cytoplasm to the nucleus. |
| protein targeting to membrane | The process of directing proteins towards a membrane, usually using signals contained within the protein. |
30 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| C1JZ66 | KPNA7 | Importin subunit alpha-8 | Bos taurus (Bovine) | PR |
| A2VE08 | KPNA1 | Importin subunit alpha-5 | Bos taurus (Bovine) | SS |
| Q0V7M0 | KPNA6 | Importin subunit alpha-7 | Bos taurus (Bovine) | SS |
| Q5ZML1 | KPNA1 | Importin subunit alpha-5 | Gallus gallus (Chicken) | SS |
| P52295 | Pen | Importin subunit alpha | Drosophila melanogaster (Fruit fly) | SS |
| O00629 | KPNA4 | Importin subunit alpha-3 | Homo sapiens (Human) | SS |
| O00505 | KPNA3 | Importin subunit alpha-4 | Homo sapiens (Human) | SS |
| A9QM74 | KPNA7 | Importin subunit alpha-8 | Homo sapiens (Human) | EV |
| P52292 | KPNA2 | Importin subunit alpha-1 | Homo sapiens (Human) | EV |
| P52294 | KPNA1 | Importin subunit alpha-5 | Homo sapiens (Human) | SS |
| O15131 | KPNA5 | Importin subunit alpha-6 | Homo sapiens (Human) | SS |
| O60684 | KPNA6 | Importin subunit alpha-7 | Homo sapiens (Human) | SS |
| O35343 | Kpna4 | Importin subunit alpha-3 | Mus musculus (Mouse) | SS |
| O35344 | Kpna3 | Importin subunit alpha-4 | Mus musculus (Mouse) | SS |
| C0LLJ0 | Kpna7 | Importin subunit alpha-8 | Mus musculus (Mouse) | SS |
| P52293 | Kpna2 | Importin subunit alpha-1 | Mus musculus (Mouse) | EV |
| Q60960 | Kpna1 | Importin subunit alpha-5 | Mus musculus (Mouse) | SS |
| O35345 | Kpna6 | Importin subunit alpha-7 | Mus musculus (Mouse) | PR |
| C6K7I2 | KPNA7 | Importin subunit alpha-8 | Sus scrofa (Pig) | SS |
| P83953 | Kpna1 | Importin subunit alpha-5 | Rattus norvegicus (Rat) | SS |
| Q56R16 | Kpna5 | Importin subunit alpha-6 | Rattus norvegicus (Rat) | SS |
| Q71VM4 | Os01g0253300 | Importin subunit alpha-1a | Oryza sativa subsp. japonica (Rice) | SS |
| Q19969 | ima-3 | Importin subunit alpha-3 | Caenorhabditis elegans | SS |
| P91276 | ima-2 | Importin subunit alpha-2 | Caenorhabditis elegans | SS |
| O04294 | IMPA3 | Importin subunit alpha-3 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9FWY7 | IMPA6 | Importin subunit alpha-6 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9M9X7 | IMPA7 | Importin subunit alpha-7 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9FJ09 | IMPA5 | Importin subunit alpha-5 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| O22478 | Importin subunit alpha | Solanum lycopersicum (Tomato) (Lycopersicon esculentum) | SS | |
| Q503E9 | kpna5 | Importin subunit alpha-6 | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MDNGTDSSTS | KFVPEYRRTN | FKNKGRFSAD | ELRRRRDTQQ | VELRKAKRDE | ALAKRRNFIP |
| 70 | 80 | 90 | 100 | 110 | 120 |
| PTDGADSDEE | DESSVSADQQ | FYSQLQQELP | QMTQQLNSDD | MQEQLSATVK | FRQILSREHR |
| 130 | 140 | 150 | 160 | 170 | 180 |
| PPIDVVIQAG | VVPRLVEFMR | ENQPEMLQLE | AAWALTNIAS | GTSAQTKVVV | DADAVPLFIQ |
| 190 | 200 | 210 | 220 | 230 | 240 |
| LLYTGSVEVK | EQAIWALGNV | AGDSTDYRDY | VLQCNAMEPI | LGLFNSNKPS | LIRTATWTLS |
| 250 | 260 | 270 | 280 | 290 | 300 |
| NLCRGKKPQP | DWSVVSQALP | TLAKLIYSMD | TETLVDACWA | ISYLSDGPQE | AIQAVIDVRI |
| 310 | 320 | 330 | 340 | 350 | 360 |
| PKRLVELLSH | ESTLVQTPAL | RAVGNIVTGN | DLQTQVVINA | GVLPALRLLL | SSPKENIKKE |
| 370 | 380 | 390 | 400 | 410 | 420 |
| ACWTISNITA | GNTEQIQAVI | DANLIPPLVK | LLEVAEYKTK | KEACWAISNA | SSGGLQRPDI |
| 430 | 440 | 450 | 460 | 470 | 480 |
| IRYLVSQGCI | KPLCDLLEIA | DNRIIEVTLD | ALENILKMGE | ADKEARGLNI | NENADFIEKA |
| 490 | 500 | 510 | 520 | 530 | 540 |
| GGMEKIFNCQ | QNENDKIYEK | AYKIIETYFG | EEEDAVDETM | APQNAGNTFG | FGSNVNQQFN |
| FN |