Q02445
SS
Gene name |
Tfpi |
Protein name |
Tissue factor pathway inhibitor |
Names |
TFPI , Extrinsic pathway inhibitor , EPI , Lipoprotein-associated coagulation inhibitor , LACI |
Species |
Rattus norvegicus (Rat) |
KEGG Pathway |
rno:29436 |
EC number |
|
Protein Class |
|
Descriptions
Kunitz-type protease inhibitor 2 (or hepatocyte growth factor activator inhibitor type 2 (HAI-2)) is an integral membrane Kunitz-type serine protease inhibitor that regulates the proteolysis of matriptase and prostasin in a cell-type selective manner. The vast majority of non-glycosylated HAI-2 is synthesized into multiple disulfide-linked oligomers, which lack protease inhibitory function, likely due to distorted conformations caused by the disarrayed disulfide linkages. N-glycosylation on Asn-57 is required for folding into a functional HAI-2 with full protease suppressive activity and correct subcellular targeting signal.
Autoinhibitory domains (AIDs)
Target domain |
50-104 (Pancreatic trypsin inhibitor Kunitz domain) |
Relief mechanism |
PTM |
Assay |
|
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
1 structures for Q02445
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q02445-F1 | Predicted | AlphaFoldDB |
1 variants for Q02445
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs197267875 | 187 | V>I | No | EVA |
No associated diseases with Q02445
6 regional properties for Q02445
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Pancreatic trypsin inhibitor Kunitz domain | 50 - 104 | IPR002223-1 |
| domain | Pancreatic trypsin inhibitor Kunitz domain | 122 - 175 | IPR002223-2 |
| domain | Pancreatic trypsin inhibitor Kunitz domain | 220 - 273 | IPR002223-3 |
| conserved_site | Proteinase inhibitor I2, Kunitz, conserved site | 81 - 99 | IPR020901-1 |
| conserved_site | Proteinase inhibitor I2, Kunitz, conserved site | 152 - 170 | IPR020901-2 |
| conserved_site | Proteinase inhibitor I2, Kunitz, conserved site | 250 - 268 | IPR020901-3 |
3 GO annotations of cellular component
| Name | Definition |
|---|---|
| caveola | A membrane raft that forms small pit, depression, or invagination that communicates with the outside of a cell and extends inward, indenting the cytoplasm and the cell membrane. Examples include flask-shaped invaginations of the plasma membrane in adipocytes associated with caveolin proteins, and minute pits or incuppings of the cell membrane formed during pinocytosis. Caveolae may be pinched off to form free vesicles within the cytoplasm. |
| cell surface | The external part of the cell wall and/or plasma membrane. |
| extracellular space | That part of a multicellular organism outside the cells proper, usually taken to be outside the plasma membranes, and occupied by fluid. |
1 GO annotations of molecular function
| Name | Definition |
|---|---|
| serine-type endopeptidase inhibitor activity | Binds to and stops, prevents or reduces the activity of a serine-type endopeptidase. |
7 GO annotations of biological process
| Name | Definition |
|---|---|
| blood coagulation | The sequential process in which the multiple coagulation factors of the blood interact, ultimately resulting in the formation of an insoluble fibrin clot; it may be divided into three stages |
| cellular response to interleukin-1 | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an interleukin-1 stimulus. |
| cellular response to lipopolysaccharide | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a lipopolysaccharide stimulus; lipopolysaccharide is a major component of the cell wall of gram-negative bacteria. |
| cellular response to steroid hormone stimulus | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a steroid hormone stimulus. |
| negative regulation of blood coagulation | Any process that stops, prevents, or reduces the frequency, rate or extent of blood coagulation. |
| response to estradiol | Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of stimulus by estradiol, a C18 steroid hormone hydroxylated at C3 and C17 that acts as a potent estrogen. |
| response to lipopolysaccharide | Any process that results in a change in state or activity of an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a lipopolysaccharide stimulus; lipopolysaccharide is a major component of the cell wall of gram-negative bacteria. |
13 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q7YRQ8 | TFPI2 | Tissue factor pathway inhibitor 2 | Bos taurus (Bovine) | SS |
| Q868Z9 | Ppn | Papilin | Drosophila melanogaster (Fruit fly) | SS |
| M9PE65 | axo | Axotactin | Drosophila melanogaster (Fruit fly) | SS |
| P49223 | SPINT3 | Kunitz-type protease inhibitor 3 | Homo sapiens (Human) | SS |
| O43291 | SPINT2 | Kunitz-type protease inhibitor 2 | Homo sapiens (Human) | EV |
| O43278 | SPINT1 | Kunitz-type protease inhibitor 1 | Homo sapiens (Human) | EV |
| P48307 | TFPI2 | Tissue factor pathway inhibitor 2 | Homo sapiens (Human) | SS |
| P10646 | TFPI | Tissue factor pathway inhibitor | Homo sapiens (Human) | SS |
| Q9R097 | Spint1 | Kunitz-type protease inhibitor 1 | Mus musculus (Mouse) | SS |
| Q9WU03 | Spint2 | Kunitz-type protease inhibitor 2 | Mus musculus (Mouse) | SS |
| O35536 | Tfpi2 | Tissue factor pathway inhibitor 2 | Mus musculus (Mouse) | SS |
| O54819 | Tfpi | Tissue factor pathway inhibitor | Mus musculus (Mouse) | SS |
| Q28864 | TFPI | Tissue factor pathway inhibitor | Macaca mulatta (Rhesus macaque) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MTNKLKKDHA | FWAAVCLLLS | IVPELLNALP | EEDDDTINTD | SELRPMKPLH | TFCAMKAEDG |
| 70 | 80 | 90 | 100 | 110 | 120 |
| PCKAMIRSYY | FNMNSHQCEE | FIYGGCRGNK | NRFDTLEECR | KTCIPGYKKT | TIKTTSGAEK |
| 130 | 140 | 150 | 160 | 170 | 180 |
| PDFCFLEEDP | GICRGFMTRY | FYNNQSKQCE | QFKYGGCLGN | SNNFETLEEC | RNTCEDPVNE |
| 190 | 200 | 210 | 220 | 230 | 240 |
| VQKGDYVTNQ | ITVTDRTTVN | NVVIPQATKA | PSQWDYDGPS | WCLEPADSGL | CKASEKRFYY |
| 250 | 260 | 270 | 280 | 290 | 300 |
| NPAIGKCRQF | NYTGCGGNNN | NFTTKQDCNR | ACKKDSSKKS | SKRAKTQRRR | KSFVKVMYEN |
| IH |