Q01314

SS
Gene name
AKT1 (PKB)
Protein name
RAC-alpha serine/threonine-protein kinase
Names
EC 2.7.11.1 , Protein kinase B , PKB , Protein kinase B alpha , PKB alpha , RAC-PK-alpha
Species
Bos taurus (Bovine)
KEGG Pathway
bta:280991
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

Akt is a serine/threonine kinase that belongs to a group of proteins called the AGC superfamily. It is involved in various biological responses through the phosphatidylinositol 3-kinase (PI3K) signal transduction pathway. Akt2, in particular, is regulated by different regions of the protein.<br>The N-terminal PH domain of Akt2 plays a regulatory role in its activation. Phosphorylation of Ser474 in the C-terminal regulatory domain is also necessary for full activation of Akt2. These modifications help activate the kinase and enable it to carry out its functions.<br>The linker region between the N-lobe and C-lobe of Akt2 occupies the ATP binding site. Additionally, the activation loop within Akt2 aids in autoinhibition by blocking the binding of peptide substrates, while still allowing access to the ATP binding site.

Autoinhibitory domains (AIDs)

144-439 (Linker region between N- and C-lobes) SS

Target domain

141-480 (Kinase domain)

Relief mechanism

PTM

Assay

7-106 (PH domain) SS

Target domain

150-408 (Protein kinase domain)

Relief mechanism

Ligand binding, PTM

Assay

Accessory elements

291-314 (Activation loop from InterPro)

Target domain

124-479 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for Q01314

Entry ID Method Resolution Chain Position Source
AF-Q01314-F1 Predicted AlphaFoldDB

No variants for Q01314

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q01314

No associated diseases with Q01314

8 regional properties for Q01314

Type Name Position InterPro Accession
domain Protein kinase domain 150 - 408 IPR000719
domain AGC-kinase, C-terminal 409 - 480 IPR000961
domain Pleckstrin homology domain 5 - 110 IPR001849
active_site Serine/threonine-protein kinase, active site 270 - 282 IPR008271
binding_site Protein kinase, ATP binding site 156 - 189 IPR017441
domain Protein kinase, C-terminal 429 - 474 IPR017892
domain Protein kinase B alpha, catalytic domain 124 - 479 IPR034676
domain Protein Kinase B, pleckstrin homology domain 4 - 111 IPR039026

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Nucleus
  • Cell membrane
  • Nucleus after activation by integrin-linked protein kinase 1 (ILK1)
  • Nuclear translocation is enhanced by interaction with TCL1A (By similarity)
  • Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus
  • Colocalizes with WDFY2 in intracellular vesicles (By similarity)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

6 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
calmodulin binding Binding to calmodulin, a calcium-binding protein with many roles, both in the calcium-bound and calcium-free states.
potassium channel activator activity Binds to and increases the activity of a potassium channel, resulting in its opening.
protein kinase activity Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions

24 GO annotations of biological process

Name Definition
apoptotic process A programmed cell death process which begins when a cell receives an internal (e.g. DNA damage) or external signal (e.g. an extracellular death ligand), and proceeds through a series of biochemical events (signaling pathway phase) which trigger an execution phase. The execution phase is the last step of an apoptotic process, and is typically characterized by rounding-up of the cell, retraction of pseudopodes, reduction of cellular volume (pyknosis), chromatin condensation, nuclear fragmentation (karyorrhexis), plasma membrane blebbing and fragmentation of the cell into apoptotic bodies. When the execution phase is completed, the cell has died.
cellular response to epidermal growth factor stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an epidermal growth factor stimulus.
cellular response to insulin stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an insulin stimulus. Insulin is a polypeptide hormone produced by the islets of Langerhans of the pancreas in mammals, and by the homologous organs of other organisms.
glucose metabolic process The chemical reactions and pathways involving glucose, the aldohexose gluco-hexose. D-glucose is dextrorotatory and is sometimes known as dextrose; it is an important source of energy for living organisms and is found free as well as combined in homo- and hetero-oligosaccharides and polysaccharides.
glycogen biosynthetic process The chemical reactions and pathways resulting in the formation of glycogen, a polydisperse, highly branched glucan composed of chains of D-glucose residues.
negative regulation of cilium assembly Any process that stops, prevents or reduces the frequency, rate or extent of cilium assembly.
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Any process that stops, prevents, or reduces the frequency, rate or extent of a cysteine-type endopeptidase activity involved in the apoptotic process.
negative regulation of protein localization to lysosome Any process that stops, prevents or reduces the frequency, rate or extent of protein localization to lysosome.
negative regulation of release of cytochrome c from mitochondria Any process that decreases the rate, frequency or extent of release of cytochrome c from mitochondria, the process in which cytochrome c is enabled to move from the mitochondrial intermembrane space into the cytosol, which is an early step in apoptosis and leads to caspase activation.
nervous system development The process whose specific outcome is the progression of nervous tissue over time, from its formation to its mature state.
organic substance transport The directed movement of organic substances into, out of or within a cell, or between cells, or within a multicellular organism by means of some agent such as a transporter or pore. An organic substance is a molecular entity that contains carbon.
peptidyl-serine phosphorylation The phosphorylation of peptidyl-serine to form peptidyl-O-phospho-L-serine.
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
positive regulation of endothelial cell proliferation Any process that activates or increases the rate or extent of endothelial cell proliferation.
positive regulation of lipid biosynthetic process Any process that activates or increases the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of lipids.
positive regulation of TORC1 signaling Any process that activates or increases the frequency, rate or extent of TORC1 signaling.
protein phosphorylation The process of introducing a phosphate group on to a protein.
regulation of apoptotic process Any process that modulates the occurrence or rate of cell death by apoptotic process.
regulation of cell migration Any process that modulates the frequency, rate or extent of cell migration.
regulation of neuron projection development Any process that modulates the rate, frequency or extent of neuron projection development. Neuron projection development is the process whose specific outcome is the progression of a neuron projection over time, from its formation to the mature structure. A neuron projection is any process extending from a neural cell, such as axons or dendrites (collectively called neurites).
regulation of translation Any process that modulates the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of proteins by the translation of mRNA or circRNA.
response to growth factor Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a growth factor stimulus.
response to growth hormone Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a growth hormone stimulus. Growth hormone is a peptide hormone that binds to the growth hormone receptor and stimulates growth.
response to insulin-like growth factor stimulus Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an insulin-like growth factor stimulus.

13 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q6TJY3 RPS6KB1 Ribosomal protein S6 kinase beta-1 Bos taurus (Bovine) SS
Q8INB9 Akt RAC serine/threonine-protein kinase Drosophila melanogaster (Fruit fly) SS
Q9Y243 AKT3 RAC-gamma serine/threonine-protein kinase Homo sapiens (Human) SS
P31751 AKT2 RAC-beta serine/threonine-protein kinase Homo sapiens (Human) EV SS
P31749 AKT1 RAC-alpha serine/threonine-protein kinase Homo sapiens (Human) EV
Q60823 Akt2 RAC-beta serine/threonine-protein kinase Mus musculus (Mouse) PR
Q9WUA6 Akt3 RAC-gamma serine/threonine-protein kinase Mus musculus (Mouse) PR
P31750 Akt1 RAC-alpha serine/threonine-protein kinase Mus musculus (Mouse) PR
P47197 Akt2 RAC-beta serine/threonine-protein kinase Rattus norvegicus (Rat) SS
Q63484 Akt3 RAC-gamma serine/threonine-protein kinase Rattus norvegicus (Rat) PR
P47196 Akt1 RAC-alpha serine/threonine-protein kinase Rattus norvegicus (Rat) PR
Q17941 akt-1 Serine/threonine-protein kinase akt-1 Caenorhabditis elegans PR
Q9XTG7 akt-2 Serine/threonine-protein kinase akt-2 Caenorhabditis elegans SS
10 20 30 40 50 60
MNDVAIVKEG WLHKRGEYIK TWRPRYFLLK NDGTFIGYKE RPQDLEQRES PLNNFSVAQC
70 80 90 100 110 120
QLMKTERPRP NTFIIRCLQW TTVIERTFHV ETPEEREEWT TAIQTVADGL KRQEEETMDF
130 140 150 160 170 180
RSGSPGENSG AEEMEVSLAK PKHRVTMNDF EYLKLLGKGT FGKVILVKEK ATGRYYAMKI
190 200 210 220 230 240
LKKEVIVAKD EVAHTLTENR VLQNSRHPFL TALKYSFQTH DRLCFVMEYA NGGELFFHLS
250 260 270 280 290 300
RERVFSEDRA RFYGAEIVSA LDYLHSEKEV VYRDLKLENL MLDKDGHIKI TDFGLCKEGI
310 320 330 340 350 360
KDGATMKTFC GTPEYLAPEV LEDNDYGRAV DWWGLGVVMY EMMCGRLPFY NQDHEKLFEL
370 380 390 400 410 420
ILMEEIRFPR TLSPEAKSLL SGLLKKDPKQ RLGGGSEDAK EIMQHRFFAS IVWQDVYEKK
430 440 450 460 470
LSPPFKPQVT SETDTRYFDE EFTAQMITIT PPDQDDSMEG VDSERRPHFP QFSYSASATA