Q007T5

SS
Gene name
ARF6
Protein name
ADP-ribosylation factor 6
Names
EC 3.6.5.2
Species
Sus scrofa (Pig)
KEGG Pathway
ssc:780425
EC number
3.6.5.2: Acting on GTP; involved in cellular and subcellular movement
Protein Class

Descriptions

Small GTPases couple their GDP/GTP structural cycle to cytosol/membrane alternation to function as versatile molecular switches in the cell. Membrane localization of their active, GTP-bound form is pivotal to their ability to propagate information, and this requires their post-translational modification by lipids.<br>Arf GTPases are modified by a myristate attached to their N-terminus, which is shielded by intramolecular interactions in their inactive state. The myristoylated N-terminus of Arf is autoinhibitory in solution and is displaced by membranes, priming Arf GTPases for activation by their GEFs. Replacement of the N-terminal myristate by a 6xHis-tag preserves autoinhibition, representing that membranes unlock the N-terminal region to facilitate subsequent activation.

Autoinhibitory domains (AIDs)

3-16 (N-terminal helix) SS

Target domain

16-138 (Small GTP-binding protein domain)

Relief mechanism

Ligand binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q007T5

Entry ID Method Resolution Chain Position Source
AF-Q007T5-F1 Predicted AlphaFoldDB

1 variants for Q007T5

Variant ID(s) Position Change Description Diseaes Association Provenance
rs703365748 146 D>Y No EVA

No associated diseases with Q007T5

1 regional properties for Q007T5

Type Name Position InterPro Accession
domain Small GTP-binding protein domain 12 - 139 IPR005225

Functions

Description
EC Number 3.6.5.2 Acting on GTP; involved in cellular and subcellular movement
Subcellular Localization
  • Cytoplasm, cytosol
  • Cell membrane ; Lipid-anchor
  • Endosome membrane ; Lipid-anchor
  • Recycling endosome membrane ; Lipid-anchor
  • Cell projection, filopodium membrane ; Lipid-anchor
  • Cell projection, ruffle
  • Cleavage furrow
  • Midbody, Midbody ring
  • Early endosome membrane ; Lipid-anchor
  • Golgi apparatus, trans-Golgi network membrane ; Lipid-anchor
  • Distributed uniformly on the plasma membrane, as well as throughout the cytoplasm during metaphase
  • Subsequently concentrated at patches in the equatorial region at the onset of cytokinesis, and becomes distributed in the equatorial region concurrent with cleavage furrow ingression
  • In late stages of cytokinesis, concentrates at the midbody ring/Flemming body (By similarity)
  • Recruitment to the midbody ring requires both activation by PSD/EFA6A and interaction with KIF23/MKLP1 (By similarity)
  • After abscission of the intercellular bridge, incorporated into one of the daughter cells as a midbody remnant and localizes to punctate structures beneath the plasma membrane (By similarity)
  • Recruited to the cell membrane in association with CYTH2 and ARL4C
  • Colocalizes with DAB2IP at the plasma membrane and endocytic vesicles (By similarity)
  • Myristoylation is required for proper localization to membranes: myristoylation on Lys-3 allows ARF6 to remain on membranes during the GTPase cycle (By similarity)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

12 GO annotations of cellular component

Name Definition
cleavage furrow The cleavage furrow is a plasma membrane invagination at the cell division site. The cleavage furrow begins as a shallow groove and eventually deepens to divide the cytoplasm.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
early endosome membrane The lipid bilayer surrounding an early endosome.
endocytic vesicle A membrane-bounded intracellular vesicle formed by invagination of the plasma membrane around an extracellular substance. Endocytic vesicles fuse with early endosomes to deliver the cargo for further sorting.
endosome A vacuole to which materials ingested by endocytosis are delivered.
filopodium membrane The portion of the plasma membrane surrounding a filopodium.
Flemming body A cell part that is the central region of the midbody characterized by a gap in alpha-tubulin staining. It is a dense structure of antiparallel microtubules from the central spindle in the middle of the intercellular bridge.
focal adhesion A cell-substrate junction that anchors the cell to the extracellular matrix and that forms a point of termination of actin filaments. In insects focal adhesion has also been referred to as hemi-adherens junction (HAJ).
Golgi apparatus A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
recycling endosome membrane The lipid bilayer surrounding a recycling endosome.
ruffle Projection at the leading edge of a crawling cell; the protrusions are supported by a microfilament meshwork.

3 GO annotations of molecular function

Name Definition
G protein activity A molecular function regulator that cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular processes. Intrinsic GTPase activity returns the G protein to its GDP-bound state. The return to the GDP-bound state can be accelerated by the action of a GTPase-activating protein (GAP).
GDP binding Binding to GDP, guanosine 5'-diphosphate.
GTP binding Binding to GTP, guanosine triphosphate.

6 GO annotations of biological process

Name Definition
cell differentiation The cellular developmental process in which a relatively unspecialized cell, e.g. embryonic or regenerative cell, acquires specialized structural and/or functional features that characterize a specific cell. Differentiation includes the processes involved in commitment of a cell to a specific fate and its subsequent development to the mature state.
intracellular protein transport The directed movement of proteins in a cell, including the movement of proteins between specific compartments or structures within a cell, such as organelles of a eukaryotic cell.
nervous system development The process whose specific outcome is the progression of nervous tissue over time, from its formation to its mature state.
protein localization to endosome A process in which a protein is transported to, or maintained in, a location within an endosome.
regulation of dendritic spine development Any process that modulates the rate, frequency, or extent of dendritic spine development, the process whose specific outcome is the progression of the dendritic spine over time, from its formation to the mature structure.
vesicle-mediated transport A cellular transport process in which transported substances are moved in membrane-bounded vesicles; transported substances are enclosed in the vesicle lumen or located in the vesicle membrane. The process begins with a step that directs a substance to the forming vesicle, and includes vesicle budding and coating. Vesicles are then targeted to, and fuse with, an acceptor membrane.

6 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P40994 ARF3 ADP-ribosylation factor 3 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SS
P26990 ARF6 ADP-ribosylation factor 6 Gallus gallus (Chicken) SS
P40946 Arf6 ADP-ribosylation factor 6 Drosophila melanogaster (Fruit fly) SS
P62330 ARF6 ADP-ribosylation factor 6 Homo sapiens (Human) EV
P62331 Arf6 ADP-ribosylation factor 6 Mus musculus (Mouse) SS
P62332 Arf6 ADP-ribosylation factor 6 Rattus norvegicus (Rat) SS
10 20 30 40 50 60
MGKVLSKIFG NKEMRILMLG LDAAGKTTIL YKLKLGQSVT TIPTVGFNVE TVTYKNVKFN
70 80 90 100 110 120
VWDVGGQDKI RPLWRHYYTG TQGLIFVVDC ADRDRIDEAR QELHRIINDR EMRDAIILIF
130 140 150 160 170
ANKQDLPDAM KPHEIQEKLG LTRIRDRNWY VQPSCATSGD GLYEGLTWLT SNYKS