P84903

SS
Gene name
Stim1
Protein name
Stromal interaction molecule 1
Names
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:361618
EC number
Protein Class

Descriptions

STIM1 stimulates calcium ion (Ca2+) entry through plasma membrane Orai1 channels in response to decreased Ca2+ concentrations in the endoplasmic reticulum lumen, and STIM1 and Orai1 are important for T cell activation, platelet aggregation and skeletal muscle development. The N-terminal luminal segment of STIM1 contains EF-hand and SAM (sterile α motif) domain and the C-terminal segment contains two putative coiled-coil domains (CC1 and CC2), as well as several acidic, serine-proline-rich, and basic segments. An acidic motif within the coiled-coil region keeps STIM1 inactive via an intramolecular interaction with a basic sequence with in CAD/SOAR that has been identified as key for Orai1 activation. The oligomerization of STIM1 unmasks the Orai1 activating domain (CAD/SOAR) by breaking the intramolecular interaction that keeps it inactive in the quiescent STIM1 molecule. Furthermore, it has been reported that stabilizing EF-SAM domains within full-length STIM1 inhibit Ca2+ release-activated Ca2+ (CRAC) entry.

Autoinhibitory domains (AIDs)

63-200 (EF-SAM domain) SS

Target domain

341-441 (SOAR domain)

Relief mechanism

Others

Assay

238-343 (Acidic motif) SS

Target domain

341-441 (SOAR domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P84903

Entry ID Method Resolution Chain Position Source
AF-P84903-F1 Predicted AlphaFoldDB

No variants for P84903

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P84903

No associated diseases with P84903

2 regional properties for P84903

Type Name Position InterPro Accession
domain Sterile alpha motif domain 129 - 200 IPR001660
domain Stromal interaction molecule, Orai1-activating region 341 - 441 IPR032393

Functions

Description
EC Number
Subcellular Localization
  • Cell membrane ; Single-pass type I membrane protein
  • Endoplasmic reticulum membrane ; Single-pass type I membrane protein
  • Sarcoplasmic reticulum
  • Cytoplasm, cytoskeleton
  • Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium and is detected at punctae corresponding to junctions between the endoplasmic reticulum and the cell membrane
  • Associated with the microtubule network at the growing distal tip of microtubules
  • Colocalizes with ORAI1 at the cell membrane
  • Colocalizes preferentially with CASQ1 at endoplasmic reticulum in response to a depletion of intracellular calcium (By similarity)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

10 GO annotations of cellular component

Name Definition
cortical endoplasmic reticulum A cortical network of highly dynamic tubules that are juxtaposed to the plasma membrane and undergo ring closure and tubule-branching movements.
endoplasmic reticulum The irregular network of unit membranes, visible only by electron microscopy, that occurs in the cytoplasm of many eukaryotic cells. The membranes form a complex meshwork of tubular channels, which are often expanded into slitlike cavities called cisternae. The ER takes two forms, rough (or granular), with ribosomes adhering to the outer surface, and smooth (with no ribosomes attached).
growth cone The migrating motile tip of a growing neuron projection, where actin accumulates, and the actin cytoskeleton is the most dynamic.
integral component of endoplasmic reticulum membrane The component of the endoplasmic reticulum membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.
integral component of plasma membrane The component of the plasma membrane consisting of the gene products and protein complexes having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.
microtubule Any of the long, generally straight, hollow tubes of internal diameter 12-15 nm and external diameter 24 nm found in a wide variety of eukaryotic cells; each consists (usually) of 13 protofilaments of polymeric tubulin, staggered in such a manner that the tubulin monomers are arranged in a helical pattern on the microtubular surface, and with the alpha/beta axes of the tubulin subunits parallel to the long axis of the tubule; exist in equilibrium with pool of tubulin monomers and can be rapidly assembled or disassembled in response to physiological stimuli; concerned with force generation, e.g. in the spindle.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
plasma membrane raft A membrane raft that is part of the plasma membrane.
protein-containing complex A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together.
sarcoplasmic reticulum membrane The lipid bilayer surrounding the sarcoplasmic reticulum.

6 GO annotations of molecular function

Name Definition
calcium channel regulator activity Modulates the activity of a calcium channel.
calcium ion binding Binding to a calcium ion (Ca2+).
identical protein binding Binding to an identical protein or proteins.
microtubule plus-end binding Binding to the plus end of a microtubule.
protease binding Binding to a protease or a peptidase.
store-operated calcium channel activity A ligand-gated ion channel activity which transports calcium in response to emptying of intracellular calcium stores.

11 GO annotations of biological process

Name Definition
activation of store-operated calcium channel activity A process that initiates the activity of an inactive store-operated calcium channel.
cation transport The directed movement of cations, atoms or small molecules with a net positive charge, into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
cellular calcium ion homeostasis Any process involved in the maintenance of an internal steady state of calcium ions at the level of a cell.
detection of calcium ion The series of events in which a calcium ion stimulus is received by a cell and converted into a molecular signal.
enamel mineralization The process in which calcium salts, mainly carbonated hydroxyapatite, are deposited in tooth enamel.
myotube differentiation The process in which a relatively unspecialized cell acquires specialized features of a myotube cell. Myotube differentiation starts with myoblast fusion and the appearance of specific cell markers (this is the cell development step). Then individual myotubes can fuse to form bigger myotubes and start to contract. Myotubes are multinucleated cells that are formed when proliferating myoblasts exit the cell cycle, differentiate and fuse.
positive regulation of adenylate cyclase activity Any process that activates or increases the frequency, rate or extent of adenylate cyclase activity.
positive regulation of angiogenesis Any process that activates or increases angiogenesis.
regulation of calcium ion transport Any process that modulates the frequency, rate or extent of the directed movement of calcium ions into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
regulation of store-operated calcium entry Any process that modulates the frequency, rate or extent of store-operated calcium entry.
store-operated calcium entry A calcium ion entry mechanism in the plasma membrane activated by the depletion of calcium ion from the internal calcium ion store in the endoplasmic reticulum.

6 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q58CP9 STIM1 Stromal interaction molecule 1 Bos taurus (Bovine) SS
P83094 Stim Stromal interaction molecule homolog Drosophila melanogaster (Fruit fly) SS
Q13586 STIM1 Stromal interaction molecule 1 Homo sapiens (Human) EV
P70302 Stim1 Stromal interaction molecule 1 Mus musculus (Mouse) SS
P83093 Stim2 Stromal interaction molecule 2 Mus musculus (Mouse) SS
G5EF60 stim-1 Stromal interaction molecule 1 Caenorhabditis elegans SS
10 20 30 40 50 60
MDVCARLALW LLWGLLLHQG QSLSHSHSEK NTGASSGATS EESTEAEFCR IDKPLCHSED
70 80 90 100 110 120
EKLSFEAVRN IHKLMDDDAN GDVDVEESDE FLREDLNYHD PTVKHSTFHG EDKLISVEDL
130 140 150 160 170 180
WKAWKASEVY NWTVDEVIQW LITYVELPQY EETFRKLQLT GHAMPRLAVT NTTMTGTVLK
190 200 210 220 230 240
MTDRSHRQKL QLKALDTVLF GPPLLTRHNH LKDFMLVVSI VIGVGGCWFA YIQNRYSKEH
250 260 270 280 290 300
MKKMMKDLEG LHRAEQSLHD LQERLHKAQE EHRTVEVEKV HLEKKLRDEI NLAKQEAQRL
310 320 330 340 350 360
KELREGTENE RSRQKYAEEE LEQVREALRK AEKELESHSS WYAPEALQKW LQLTHEVEVQ
370 380 390 400 410 420
YYNIKKQNAE RQLLVAKEGA EKIKKKRNTL FGTFHVAHSS SLDDVDHKIL TAKQALSEVT
430 440 450 460 470 480
AALRERLHRW QQIEILCGFQ IVNNPGIHSL VAALNIDPSW MGSTRPNPAH FIMTDDVDDM
490 500 510 520 530 540
DEEIVSPLSM QSPSLQSSVR QRLTEPQHGL GSQRDLTHSD SESSLHTSDR QRVAPKPPQM
550 560 570 580 590 600
GRAADEALNA TSSNGSHRLI EGVHPGSLVE KLPDSPALAK KTILALNHGL DKAHSLMELN
610 620 630 640 650 660
PSVPPGGSPL LDSSHSHSPS SPDPDTPSPV GDSRALQGSR NTRIPHLAGK KAMAEEDNGS
670 680
IGEETDSSPG RKKFPLKIFK KPLKK