P84085

EV
Gene name
ARF5
Protein name
ADP-ribosylation factor 5
Names
Species
Homo sapiens (Human)
KEGG Pathway
hsa:381
EC number
Protein Class
ADP RIBOSYLATION FACTOR-RELATED (PTHR11711)

Descriptions

Small GTPases couple their GDP/GTP structural cycle to cytosol/membrane alternation to function as versatile molecular switches in the cell. Membrane localization of their active, GTP-bound form is pivotal to their ability to propagate information, and this requires their post-translational modification by lipids.
Arf GTPases are modified by a myristate attached to their N-terminus, which is shielded by intramolecular interactions in their inactive state. The myristoylated N-terminus of Arf is autoinhibitory in solution and is displaced by membranes, priming Arf GTPases for activation by their GEFs. Replacement of the N-terminal myristate by a 6xHis-tag preserves autoinhibition, representing that membranes unlock the N-terminal region to facilitate subsequent activation.

Autoinhibitory domains (AIDs)

3-16 (N-terminal helix) EV

Target domain

16-138 (Small GTP-binding protein domain)

Relief mechanism

Ligand binding

Assay

Mutagenesis experiment

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

2 structures for P84085

Entry ID Method Resolution Chain Position Source
2B6H X-ray 176 A A 8-180 PDB
AF-P84085-F1 Predicted AlphaFoldDB

121 variants for P84085

Variant ID(s) Position Change Description Diseaes Association Provenance
rs866219932 2 G>V No Ensembl
rs1207747890 4 T>A No gnomAD
rs965631736 4 T>I No TOPMed
gnomAD
rs965631736 4 T>S No TOPMed
gnomAD
rs1794237698 5 V>M No Ensembl
rs759843060 7 A>V No ExAC
gnomAD
rs1794237834 8 L>F No TOPMed
rs1162647178 8 L>P No gnomAD
rs1383146791 9 F>I No gnomAD
rs1562953554 11 R>P No Ensembl
rs1794237934 11 R>W No Ensembl
rs769953515 12 I>V No ExAC
gnomAD
rs866714062 14 G>V No Ensembl
TCGA novel 15 K>N Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
rs1371360324 17 Q>H No gnomAD
rs775454058 18 M>I No ExAC
gnomAD
rs202016109 19 R>L No Ensembl
TCGA novel 19 R>W Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
rs1284054298 22 M>I No gnomAD
rs1449393189 22 M>L No gnomAD
TCGA novel 24 G>R Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
rs1794247263 26 D>A No TOPMed
rs866133430 27 A>V No Ensembl
rs1794247376 28 A>V No TOPMed
gnomAD
rs1587535775 30 K>Q No Ensembl
rs1587535782 31 T>N No Ensembl
rs767832041 33 I>V No ExAC
TOPMed
gnomAD
rs1794247724 36 K>R No Ensembl
rs1803150 39 L>W No Ensembl
rs1303907072 40 G>E No TOPMed
gnomAD
rs1303907072 40 G>V No TOPMed
gnomAD
rs1587535793 41 E>G No TOPMed
rs1794247884 41 E>K No gnomAD
COSM1447851 41 E>R Variant assessed as Somatic; HIGH impact. [NCI-TCGA] No NCI-TCGA Cosmic
TCGA novel 42 I>V Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
rs1794247985 44 T>S No TOPMed
rs1794248143 49 I>T No Ensembl
rs952591454 49 I>V No Ensembl
rs912240810 51 F>Y No TOPMed
gnomAD
rs754222019 61 I>T No Ensembl
rs1039475305 61 I>V No TOPMed
rs1794253997 62 C>R No TOPMed
rs1794253997 62 C>S No TOPMed
rs981999965 74 I>M No Ensembl
rs1297082014 75 R>W Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
gnomAD
COSM3877865 79 R>L Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA Cosmic
rs1794254487 83 Q>R No Ensembl
rs144009854 97 R>Q No ESP
ExAC
TOPMed
gnomAD
rs367999369 97 R>W No ESP
ExAC
TOPMed
gnomAD
COSM78870 98 E>* Variant assessed as Somatic; HIGH impact. [NCI-TCGA] No NCI-TCGA Cosmic
TCGA novel
rs1794261468
 98 E>D Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
TOPMed
COSM3698147 99 R>W Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA Cosmic
rs1794261568 101 Q>* No gnomAD
COSM1085148 102 E>* Variant assessed as Somatic; HIGH impact. [NCI-TCGA] No NCI-TCGA Cosmic
TCGA novel 103 S>F Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
rs771445967 103 S>P No ExAC
gnomAD
rs781588986 104 A>T No ExAC
gnomAD
TCGA novel 108 Q>* Variant assessed as Somatic; HIGH impact. [NCI-TCGA] No NCI-TCGA
TCGA novel 108 Q>L Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
rs1389309924 110 M>I No gnomAD
rs1794262196 110 M>L No Ensembl
rs1794262196 110 M>V No Ensembl
rs1223710373 114 D>N No gnomAD
rs1489738157 115 E>D No TOPMed
gnomAD
COSM3674819
rs780593712
 117 R>L prostate [Cosmic] No cosmic curated
ExAC
TOPMed
gnomAD
rs780593712 117 R>Q No ExAC
TOPMed
gnomAD
rs1286936912
COSM1085149
 117 R>W Variant assessed as Somatic; MODERATE impact. endometrium [NCI-TCGA, Cosmic] No NCI-TCGA Cosmic
cosmic curated
NCI-TCGA
TOPMed
gnomAD
rs1176017535 118 D>G No TOPMed
rs1233003773 119 A>T No gnomAD
rs1794279276 119 A>V No TOPMed
rs747749998 120 V>G No ExAC
TOPMed
gnomAD
rs1794279479 124 F>C No Ensembl
rs773043755
COSM318745
 125 A>S lung [Cosmic] No cosmic curated
ExAC
gnomAD
TCGA novel 129 D>G Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
rs746526652 130 M>I No ExAC
gnomAD
rs1794279688 132 N>S No TOPMed
rs201108741 133 A>T No 1000Genomes
ExAC
TOPMed
gnomAD
rs1309906980 134 M>V No TOPMed
gnomAD
TCGA novel 135 P>H Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
rs1794279862 135 P>L No TOPMed
rs759398219 135 P>T No ExAC
gnomAD
rs1278238405 136 V>M No TOPMed
gnomAD
rs775287559
TCGA novel
 137 S>R Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
ExAC
TOPMed
gnomAD
rs543068324 138 E>K No 1000Genomes
ExAC
TOPMed
gnomAD
rs543068324 138 E>Q No 1000Genomes
ExAC
TOPMed
gnomAD
rs1356798060 140 T>S No gnomAD
rs1206926592 142 K>R No gnomAD
rs1587536937 144 G>A No Ensembl
rs1273089709 148 L>F No gnomAD
rs1304516534 148 L>S No TOPMed
rs751785636 149 R>C No ExAC
TOPMed
gnomAD
rs767496095 149 R>H No ExAC
TOPMed
gnomAD
rs767496095 149 R>L No ExAC
TOPMed
gnomAD
rs751785636 149 R>S No ExAC
TOPMed
gnomAD
rs916226462 150 S>G No TOPMed
gnomAD
rs750899407 151 R>C No ExAC
gnomAD
rs756706436 151 R>H No ExAC
TOPMed
gnomAD
rs756706436 151 R>L No ExAC
TOPMed
gnomAD
rs749643254 152 T>K No ExAC
gnomAD
rs749643254 152 T>M Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No ExAC
NCI-TCGA
gnomAD
COSM1085150 155 V>I Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA Cosmic
rs1222389800 157 A>T Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
gnomAD
rs1261127308 157 A>V No gnomAD
rs780948404 158 T>I No ExAC
TOPMed
gnomAD
rs780948404 158 T>S No ExAC
TOPMed
gnomAD
rs2117418854 162 Q>P No Ensembl
rs1794283006 164 T>K No TOPMed
gnomAD
TCGA novel 165 G>C Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
rs1480770986 168 D>N Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA
TOPMed
gnomAD
rs2117418891 171 D>H No Ensembl
COSM744675 172 W>L Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No NCI-TCGA Cosmic
rs1794283697 174 S>C No TOPMed
rs1794283697 174 S>F No TOPMed
rs142827438 175 H>Q No ESP
ExAC
TOPMed
gnomAD
rs774035808 176 E>K Variant assessed as Somatic; MODERATE impact. [NCI-TCGA] No ExAC
NCI-TCGA
TOPMed
gnomAD
rs1261768055 178 S>* No TOPMed
gnomAD
rs944602969 178 S>A No TOPMed
gnomAD
rs772150653 179 K>N No ExAC
gnomAD
rs773554378
COSM1085151
 180 R>C ovary Variant assessed as Somatic; MODERATE impact. endometrium [Cosmic, NCI-TCGA] No NCI-TCGA Cosmic
cosmic curated
ExAC
NCI-TCGA
TOPMed
gnomAD
COSM1447853
rs760826467
 180 R>H Variant assessed as Somatic; MODERATE impact. large_intestine [NCI-TCGA, Cosmic] No NCI-TCGA Cosmic
cosmic curated
ExAC
NCI-TCGA
gnomAD
rs1587537120 181 R>Y No Ensembl

No associated diseases with P84085

1 regional properties for P84085

Type Name Position InterPro Accession
domain Small GTP-binding protein domain 16 - 138 IPR005225

Functions

Description
EC Number
Subcellular Localization
  • Golgi apparatus
  • Cytoplasm, perinuclear region
  • Membrane ; Lipid-anchor
  • Golgi apparatus, trans-Golgi network membrane ; Lipid-anchor
PANTHER Family PTHR11711 ADP RIBOSYLATION FACTOR-RELATED
PANTHER Subfamily PTHR11711:SF323 ADP-RIBOSYLATION FACTOR 5
PANTHER Protein Class G-protein
PANTHER Pathway Category Huntington disease
ARF

4 GO annotations of cellular component

Name Definition
extracellular exosome A vesicle that is released into the extracellular region by fusion of the limiting endosomal membrane of a multivesicular body with the plasma membrane. Extracellular exosomes, also simply called exosomes, have a diameter of about 40-100 nm.
Golgi apparatus A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways.
perinuclear region of cytoplasm Cytoplasm situated near, or occurring around, the nucleus.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

2 GO annotations of molecular function

Name Definition
GTP binding Binding to GTP, guanosine triphosphate.
GTPase activity Catalysis of the reaction

3 GO annotations of biological process

Name Definition
intracellular protein transport The directed movement of proteins in a cell, including the movement of proteins between specific compartments or structures within a cell, such as organelles of a eukaryotic cell.
retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum The directed movement of substances from the Golgi back to the endoplasmic reticulum, mediated by vesicles bearing specific protein coats such as COPI or COG.
vesicle-mediated transport A cellular transport process in which transported substances are moved in membrane-bounded vesicles; transported substances are enclosed in the vesicle lumen or located in the vesicle membrane. The process begins with a step that directs a substance to the forming vesicle, and includes vesicle budding and coating. Vesicles are then targeted to, and fuse with, an acceptor membrane.

48 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q2KJ96 ARL5A ADP-ribosylation factor-like protein 5A Bos taurus (Bovine) SS
P84081 ARF2 ADP-ribosylation factor 2 Bos taurus (Bovine) SS
P84080 ARF1 ADP-ribosylation factor 1 Bos taurus (Bovine) SS
Q3SZF2 ARF4 ADP-ribosylation factor 4 Bos taurus (Bovine) SS
Q0VC18 ARL4D ADP-ribosylation factor-like protein 4D Bos taurus (Bovine) PR
P49702 ARF5 ADP-ribosylation factor 5 Gallus gallus (Chicken) SS
P61209 Arf1 ADP-ribosylation factor 1 Drosophila melanogaster (Fruit fly) SS
P40945 Arf4 ADP ribosylation factor 4 Drosophila melanogaster (Fruit fly) SS
A6NH57 ARL5C Putative ADP-ribosylation factor-like protein 5C Homo sapiens (Human) SS
P18085 ARF4 ADP-ribosylation factor 4 Homo sapiens (Human) EV
P49703 ARL4D ADP-ribosylation factor-like protein 4D Homo sapiens (Human) PR
P84077 ARF1 ADP-ribosylation factor 1 Homo sapiens (Human) EV
Q8N4G2 ARL14 ADP-ribosylation factor-like protein 14 Homo sapiens (Human) SS
Q969Q4 ARL11 ADP-ribosylation factor-like protein 11 Homo sapiens (Human) PR
Q96KC2 ARL5B ADP-ribosylation factor-like protein 5B Homo sapiens (Human) SS
P62330 ARF6 ADP-ribosylation factor 6 Homo sapiens (Human) EV
Q8IVW1 ARL17A ADP-ribosylation factor-like protein 17 Homo sapiens (Human) SS
Q9H0F7 ARL6 ADP-ribosylation factor-like protein 6 Homo sapiens (Human) SS
P40616 ARL1 ADP-ribosylation factor-like protein 1 Homo sapiens (Human) SS
P61204 ARF3 ADP-ribosylation factor 3 Homo sapiens (Human) EV
Q9Y689 ARL5A ADP-ribosylation factor-like protein 5A Homo sapiens (Human) SS
P49076 ARF1 ADP-ribosylation factor Zea mays (Maize) SS
P61750 Arf4 ADP-ribosylation factor 4 Mus musculus (Mouse) SS
Q99PE9 Arl4d ADP-ribosylation factor-like protein 4D Mus musculus (Mouse) PR
Q6P068 Arl5c ADP-ribosylation factor-like protein 5C Mus musculus (Mouse) SS
Q80ZU0 Arl5a ADP-ribosylation factor-like protein 5A Mus musculus (Mouse) SS
P61205 Arf3 ADP-ribosylation factor 3 Mus musculus (Mouse) SS
Q9D4P0 Arl5b ADP-ribosylation factor-like protein 5B Mus musculus (Mouse) SS
P84078 Arf1 ADP-ribosylation factor 1 Mus musculus (Mouse) SS
Q8BSL7 Arf2 ADP-ribosylation factor 2 Mus musculus (Mouse) SS
P84084 Arf5 ADP-ribosylation factor 5 Mus musculus (Mouse) SS
P51824 ADP-ribosylation factor 1 Solanum tuberosum (Potato) SS
P84082 Arf2 ADP-ribosylation factor 2 Rattus norvegicus (Rat) SS
P51646 Arl5a ADP-ribosylation factor-like protein 5A Rattus norvegicus (Rat) SS
P36407 Trim23 E3 ubiquitin-protein ligase TRIM23 Rattus norvegicus (Rat) SS
P61751 Arf4 ADP-ribosylation factor 4 Rattus norvegicus (Rat) SS
P61206 Arf3 ADP-ribosylation factor 3 Rattus norvegicus (Rat) SS
P84079 Arf1 ADP-ribosylation factor 1 Rattus norvegicus (Rat) SS
P84083 Arf5 ADP-ribosylation factor 5 Rattus norvegicus (Rat) SS
P51823 ARF ADP-ribosylation factor 2 Oryza sativa subsp. japonica (Rice) SS
Q06396 Os01g0813400 ADP-ribosylation factor 1 Oryza sativa subsp. japonica (Rice) SS
Q10943 arf-1.2 ADP-ribosylation factor 1-like 2 Caenorhabditis elegans SS
P34212 arl-5 ADP-ribosylation factor-like protein 5 Caenorhabditis elegans SS
P40940 ARF3 ADP-ribosylation factor 3 Arabidopsis thaliana (Mouse-ear cress) SS
P36397 ARF1 ADP-ribosylation factor 1 Arabidopsis thaliana (Mouse-ear cress) SS
P0DH91 ARF2-B ADP-ribosylation factor 2-B Arabidopsis thaliana (Mouse-ear cress) SS
Q9LQC8 ARF2-A ADP-ribosylation factor 2-A Arabidopsis thaliana (Mouse-ear cress) SS
Q5M9P8 arl6 ADP-ribosylation factor-like protein 6 Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MGLTVSALFS RIFGKKQMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN
70 80 90 100 110 120
ICFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV QESADELQKM LQEDELRDAV
130 140 150 160 170
LLVFANKQDM PNAMPVSELT DKLGLQHLRS RTWYVQATCA TQGTGLYDGL DWLSHELSKR