P84080
SS
Gene name |
ARF1 |
Protein name |
ADP-ribosylation factor 1 |
Names |
EC 3.6.5.2 |
Species |
Bos taurus (Bovine) |
KEGG Pathway |
bta:338058 |
EC number |
3.6.5.2: Acting on GTP; involved in cellular and subcellular movement |
Protein Class |
|
Descriptions
Small GTPases couple their GDP/GTP structural cycle to cytosol/membrane alternation to function as versatile molecular switches in the cell. Membrane localization of their active, GTP-bound form is pivotal to their ability to propagate information, and this requires their post-translational modification by lipids.<br>Arf GTPases are modified by a myristate attached to their N-terminus, which is shielded by intramolecular interactions in their inactive state. The myristoylated N-terminus of Arf is autoinhibitory in solution and is displaced by membranes, priming Arf GTPases for activation by their GEFs. Replacement of the N-terminal myristate by a 6xHis-tag preserves autoinhibition, representing that membranes unlock the N-terminal region to facilitate subsequent activation.
Autoinhibitory domains (AIDs)
Target domain |
16-138 (Small GTP-binding protein domain) |
Relief mechanism |
Ligand binding |
Assay |
|
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
5 structures for P84080
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 1R8Q | X-ray | 186 A | A/B | 1-181 | PDB |
| 1R8S | X-ray | 146 A | A | 18-181 | PDB |
| 1S9D | X-ray | 180 A | A | 18-181 | PDB |
| 4C0A | X-ray | 330 A | C/D/G/H | 18-181 | PDB |
| AF-P84080-F1 | Predicted | AlphaFoldDB |
45 variants for P84080
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs480927530 | 4 | I>L | No | EVA | |
| rs446420899 | 7 | N>I | No | EVA | |
| rs457783096 | 8 | L>H | No | EVA | |
| rs477818380 | 8 | L>I | No | EVA | |
| rs481963798 | 11 | G>V | No | EVA | |
| rs441829434 | 12 | L>I | No | EVA | |
| rs473297564 | 13 | F>V | No | EVA | |
| rs440317988 | 16 | K>T | No | EVA | |
| rs446680932 | 24 | G>A | No | EVA | |
| rs446680932 | 24 | G>V | No | EVA | |
| rs438051589 | 26 | D>G | No | EVA | |
| rs469483218 | 28 | A>G | No | EVA | |
| rs455898426 | 29 | G>E | No | EVA | |
| rs467260574 | 39 | L>Q | No | EVA | |
| rs435797299 | 39 | L>V | No | EVA | |
| rs477755453 | 45 | T>P | No | EVA | |
| rs464146283 | 50 | G>S | No | EVA | |
| rs475915114 | 85 | T>P | No | EVA | |
| rs445831773 | 91 | V>A | No | EVA | |
| rs477090327 | 95 | N>I | No | EVA | |
| rs463499184 | 96 | D>V | No | EVA | |
| rs444392081 | 97 | R>S | No | EVA | |
| rs475809417 | 98 | E>A | No | EVA | |
| rs462211770 | 102 | E>V | No | EVA | |
| rs442083833 | 103 | A>G | No | EVA | |
| rs453513920 | 104 | R>L | No | EVA | |
| rs453513920 | 104 | R>P | No | EVA | |
| rs473573936 | 104 | R>S | No | EVA | |
| rs433373002 | 105 | E>A | No | EVA | |
| rs433373002 | 105 | E>G | No | EVA | |
| rs471265443 | 106 | E>A | No | EVA | |
| rs457634336 | 106 | E>D | No | EVA | |
| rs468139606 | 110 | M>R | No | EVA | |
| rs434461566 | 111 | L>V | No | EVA | |
| rs445770040 | 113 | E>* | No | EVA | |
| rs445770040 | 113 | E>Q | No | EVA | |
| rs477028991 | 114 | D>G | No | EVA | |
| rs449911925 | 115 | E>D | No | EVA | |
| rs463436083 | 115 | E>G | No | EVA | |
| rs481401825 | 118 | D>G | No | EVA | |
| rs462117954 | 119 | A>P | No | EVA | |
| rs473460744 | 127 | K>N | No | EVA | |
| rs459860847 | 128 | Q>* | No | EVA | |
| rs440716884 | 153 | W>G | No | EVA | |
| rs472011427 | 173 | L>P | No | EVA |
No associated diseases with P84080
1 regional properties for P84080
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Small GTP-binding protein domain | 16 - 141 | IPR005225 |
Functions
| Description | ||
|---|---|---|
| EC Number | 3.6.5.2 | Acting on GTP; involved in cellular and subcellular movement |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
6 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| Golgi apparatus | A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways. |
| Golgi membrane | The lipid bilayer surrounding any of the compartments of the Golgi apparatus. |
| neuron projection | A prolongation or process extending from a nerve cell, e.g. an axon or dendrite. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
| postsynaptic density | An electron dense network of proteins within and adjacent to the postsynaptic membrane of an asymmetric, neuron-neuron synapse. Its major components include neurotransmitter receptors and the proteins that spatially and functionally organize them such as anchoring and scaffolding molecules, signaling enzymes and cytoskeletal components. |
2 GO annotations of molecular function
| Name | Definition |
|---|---|
| GTP binding | Binding to GTP, guanosine triphosphate. |
| GTPase activity | Catalysis of the reaction |
6 GO annotations of biological process
| Name | Definition |
|---|---|
| dendritic spine organization | A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of a dendritic spine. A dendritic spine is a specialized protrusion from a neuronal dendrite and is involved in synaptic transmission. |
| intracellular protein transport | The directed movement of proteins in a cell, including the movement of proteins between specific compartments or structures within a cell, such as organelles of a eukaryotic cell. |
| long-term synaptic depression | A process that modulates synaptic plasticity such that synapses are changed resulting in the decrease in the rate, or frequency of synaptic transmission at the synapse. |
| regulation of Arp2/3 complex-mediated actin nucleation | Any process that modulates the frequency, rate or extent of actin nucleation mediated by the Arp2/3 complex and interacting proteins. |
| regulation of receptor internalization | Any process that modulates the frequency, rate or extent of receptor internalization. |
| vesicle-mediated transport | A cellular transport process in which transported substances are moved in membrane-bounded vesicles; transported substances are enclosed in the vesicle lumen or located in the vesicle membrane. The process begins with a step that directs a substance to the forming vesicle, and includes vesicle budding and coating. Vesicles are then targeted to, and fuse with, an acceptor membrane. |
47 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P84081 | ARF2 | ADP-ribosylation factor 2 | Bos taurus (Bovine) | SS |
| Q0VC18 | ARL4D | ADP-ribosylation factor-like protein 4D | Bos taurus (Bovine) | PR |
| Q2KJ96 | ARL5A | ADP-ribosylation factor-like protein 5A | Bos taurus (Bovine) | SS |
| Q3SZF2 | ARF4 | ADP-ribosylation factor 4 | Bos taurus (Bovine) | SS |
| Q5E9I6 | ARF3 | ADP-ribosylation factor 3 | Bos taurus (Bovine) | SS |
| P49702 | ARF5 | ADP-ribosylation factor 5 | Gallus gallus (Chicken) | SS |
| P40945 | Arf4 | ADP ribosylation factor 4 | Drosophila melanogaster (Fruit fly) | SS |
| P61209 | Arf1 | ADP-ribosylation factor 1 | Drosophila melanogaster (Fruit fly) | SS |
| P84085 | ARF5 | ADP-ribosylation factor 5 | Homo sapiens (Human) | EV |
| P61204 | ARF3 | ADP-ribosylation factor 3 | Homo sapiens (Human) | EV |
| A6NH57 | ARL5C | Putative ADP-ribosylation factor-like protein 5C | Homo sapiens (Human) | SS |
| Q969Q4 | ARL11 | ADP-ribosylation factor-like protein 11 | Homo sapiens (Human) | PR |
| Q9Y689 | ARL5A | ADP-ribosylation factor-like protein 5A | Homo sapiens (Human) | SS |
| P18085 | ARF4 | ADP-ribosylation factor 4 | Homo sapiens (Human) | EV |
| P49703 | ARL4D | ADP-ribosylation factor-like protein 4D | Homo sapiens (Human) | PR |
| Q8N4G2 | ARL14 | ADP-ribosylation factor-like protein 14 | Homo sapiens (Human) | SS |
| Q8IVW1 | ARL17A | ADP-ribosylation factor-like protein 17 | Homo sapiens (Human) | SS |
| Q9H0F7 | ARL6 | ADP-ribosylation factor-like protein 6 | Homo sapiens (Human) | SS |
| Q96KC2 | ARL5B | ADP-ribosylation factor-like protein 5B | Homo sapiens (Human) | SS |
| P84077 | ARF1 | ADP-ribosylation factor 1 | Homo sapiens (Human) | EV |
| P49076 | ARF1 | ADP-ribosylation factor | Zea mays (Maize) | SS |
| P84084 | Arf5 | ADP-ribosylation factor 5 | Mus musculus (Mouse) | SS |
| P61750 | Arf4 | ADP-ribosylation factor 4 | Mus musculus (Mouse) | SS |
| Q99PE9 | Arl4d | ADP-ribosylation factor-like protein 4D | Mus musculus (Mouse) | PR |
| Q6P068 | Arl5c | ADP-ribosylation factor-like protein 5C | Mus musculus (Mouse) | SS |
| Q80ZU0 | Arl5a | ADP-ribosylation factor-like protein 5A | Mus musculus (Mouse) | SS |
| P61205 | Arf3 | ADP-ribosylation factor 3 | Mus musculus (Mouse) | SS |
| Q9D4P0 | Arl5b | ADP-ribosylation factor-like protein 5B | Mus musculus (Mouse) | SS |
| Q8BSL7 | Arf2 | ADP-ribosylation factor 2 | Mus musculus (Mouse) | SS |
| P84078 | Arf1 | ADP-ribosylation factor 1 | Mus musculus (Mouse) | SS |
| P51824 | ADP-ribosylation factor 1 | Solanum tuberosum (Potato) | SS | |
| P84082 | Arf2 | ADP-ribosylation factor 2 | Rattus norvegicus (Rat) | SS |
| P51646 | Arl5a | ADP-ribosylation factor-like protein 5A | Rattus norvegicus (Rat) | SS |
| P84083 | Arf5 | ADP-ribosylation factor 5 | Rattus norvegicus (Rat) | SS |
| P36407 | Trim23 | E3 ubiquitin-protein ligase TRIM23 | Rattus norvegicus (Rat) | SS |
| P61751 | Arf4 | ADP-ribosylation factor 4 | Rattus norvegicus (Rat) | SS |
| P61206 | Arf3 | ADP-ribosylation factor 3 | Rattus norvegicus (Rat) | SS |
| P84079 | Arf1 | ADP-ribosylation factor 1 | Rattus norvegicus (Rat) | SS |
| P51823 | ARF | ADP-ribosylation factor 2 | Oryza sativa subsp. japonica (Rice) | SS |
| Q06396 | Os01g0813400 | ADP-ribosylation factor 1 | Oryza sativa subsp. japonica (Rice) | SS |
| P34212 | arl-5 | ADP-ribosylation factor-like protein 5 | Caenorhabditis elegans | SS |
| Q10943 | arf-1.2 | ADP-ribosylation factor 1-like 2 | Caenorhabditis elegans | SS |
| P40940 | ARF3 | ADP-ribosylation factor 3 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| P0DH91 | ARF2-B | ADP-ribosylation factor 2-B | Arabidopsis thaliana (Mouse-ear cress) | SS |
| P36397 | ARF1 | ADP-ribosylation factor 1 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9LQC8 | ARF2-A | ADP-ribosylation factor 2-A | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q5M9P8 | arl6 | ADP-ribosylation factor-like protein 6 | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MGNIFANLFK | GLFGKKEMRI | LMVGLDAAGK | TTILYKLKLG | EIVTTIPTIG | FNVETVEYKN |
| 70 | 80 | 90 | 100 | 110 | 120 |
| ISFTVWDVGG | QDKIRPLWRH | YFQNTQGLIF | VVDSNDRERV | NEAREELMRM | LAEDELRDAV |
| 130 | 140 | 150 | 160 | 170 | 180 |
| LLVFANKQDL | PNAMNAAEIT | DKLGLHSLRH | RNWYIQATCA | TSGDGLYEGL | DWLSNQLRNQ |
| K |