P68403

SS
Gene name
Prkcb (Pkcb, Prkcb1)
Protein name
Protein kinase C beta type
Names
PKC-B , PKC-beta , EC 2.7.11.13
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:25023
EC number
2.7.11.13: Protein-serine/threonine kinases
Protein Class

Descriptions

PRKCA encodes for Protein kinase C (PKC) alpha type belonging to the PCKs family and is involved in various cellular processes, including cell growth, immune response, muscle contraction, and synaptic plasticity. A well-known autoinhibitory region of the PKC family is a pseudosubstrate sequence at residues 1-30 that directly interacts with and blocks the catalytic activity of the kinase domain. Subsequences of the pseudosubstrate region are the C1 domains (C1A and C1B that bind diacylglycerol (DAG)), and the C2 domain (which binds to anionic phospholipids in a Ca2+-dependent manner). The C1A (residues 31-100) domain coordinates a network of domain-domain intramolecular interactions, including direct C1A-C2 contacts. The pseudosubstrate and C1A domains function as a single functional unit and are required to maintain autoinhibition. Additionally, the absence of the C1A domain significantly increases basal activity.

Autoinhibitory domains (AIDs)

101-151 (C1B domain) SS

Target domain

344-586 (Protein kinase domain)

Relief mechanism

Ligand binding

Assay

152-177 (C1B-C2 linker) SS

Target domain

344-586 (Protein kinase domain)

Relief mechanism

Ligand binding

Assay

Accessory elements

483-506 (Activation loop from InterPro)

Target domain

341-664 (Catalytic domain of the Serine/Threonine Kinase, Classical Protein Kinase C beta)

Relief mechanism

Assay

483-506 (Activation loop from InterPro)

Target domain

341-664 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

3 structures for P68403

Entry ID Method Resolution Chain Position Source
1A25 X-ray 270 A A/B 154-289 PDB
3PFQ X-ray 400 A A 1-661 PDB
AF-P68403-F1 Predicted AlphaFoldDB

No variants for P68403

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P68403

No associated diseases with P68403

6 regional properties for P68403

Type Name Position InterPro Accession
domain DEP domain 216 - 291 IPR000591
domain Cyclic nucleotide-binding domain 43 - 162 IPR000595-1
domain Cyclic nucleotide-binding domain 356 - 475 IPR000595-2
domain Ras-like guanine nucleotide exchange factor, N-terminal 495 - 634 IPR000651
domain Ras guanine-nucleotide exchange factors catalytic domain 768 - 1010 IPR001895
conserved_site Ras guanine-nucleotide exchange factor, conserved site 921 - 950 IPR019804

Functions

Description
EC Number 2.7.11.13 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Nucleus
  • Membrane; Peripheral membrane protein
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

11 GO annotations of cellular component

Name Definition
brush border membrane The portion of the plasma membrane surrounding the brush border.
calyx of Held The terminal specialization of a calyciferous axon which forms large synapses in the mammalian auditory central nervous system.
centrosome A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
membrane A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
presynapse The part of a synapse that is part of the presynaptic cell.
presynaptic cytosol The region of the cytosol consisting of all cytosol that is part of the presynapse.
spectrin Membrane associated dimeric protein (240 and 220 kDa) of erythrocytes. Forms a complex with ankyrin, actin and probably other components of the membrane cytoskeleton, so that there is a mesh of proteins underlying the plasma membrane, potentially restricting the lateral mobility of integral proteins.

12 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
calcium channel regulator activity Modulates the activity of a calcium channel.
chromatin binding Binding to chromatin, the network of fibers of DNA, protein, and sometimes RNA, that make up the chromosomes of the eukaryotic nucleus during interphase.
diacylglycerol-dependent serine/threonine kinase activity Catalysis of the reaction
histone binding Binding to a histone, any of a group of water-soluble proteins found in association with the DNA of eukaryotic or archaeal chromosomes. They are involved in the condensation and coiling of chromosomes during cell division and have also been implicated in gene regulation and DNA replication. They may be chemically modified (methylated, acetlyated and others) to regulate gene transcription.
histone H3T6 kinase activity Catalysis of the reaction
nuclear androgen receptor binding Binding to a nuclear androgen receptor.
nuclear receptor coactivator activity A transcription coactivator activity that activates or increases the transcription of specific gene sets via binding to a DNA-bound nuclear receptor, either on its own or as part of a complex. Coactivators often act by altering chromatin structure and modifications. For example, one class of transcription coregulators modifies chromatin structure through covalent modification of histones. A second class remodels the conformation of chromatin in an ATP-dependent fashion. A third class modulates interactions of DNA-bound DNA-binding transcription factors with other transcription coregulators. A fourth class of coactivator activity is the bridging of a DNA-binding transcription factor to the general (basal) transcription machinery. The Mediator complex, which bridges sequence-specific DNA binding transcription factors and RNA polymerase, is also a transcription coactivator.
protein kinase C binding Binding to protein kinase C.
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions
zinc ion binding Binding to a zinc ion (Zn).

30 GO annotations of biological process

Name Definition
adaptive immune response An immune response mediated by cells expressing specific receptors for antigens produced through a somatic diversification process, and allowing for an enhanced secondary response to subsequent exposures to the same antigen (immunological memory).
apoptotic process A programmed cell death process which begins when a cell receives an internal (e.g. DNA damage) or external signal (e.g. an extracellular death ligand), and proceeds through a series of biochemical events (signaling pathway phase) which trigger an execution phase. The execution phase is the last step of an apoptotic process, and is typically characterized by rounding-up of the cell, retraction of pseudopodes, reduction of cellular volume (pyknosis), chromatin condensation, nuclear fragmentation (karyorrhexis), plasma membrane blebbing and fragmentation of the cell into apoptotic bodies. When the execution phase is completed, the cell has died.
B cell activation The change in morphology and behavior of a mature or immature B cell resulting from exposure to a mitogen, cytokine, chemokine, cellular ligand, or an antigen for which it is specific.
B cell receptor signaling pathway The series of molecular signals initiated by the cross-linking of an antigen receptor on a B cell.
calcium ion transport The directed movement of calcium (Ca) ions into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
cellular response to carbohydrate stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a carbohydrate stimulus.
dibenzo-p-dioxin metabolic process The chemical reactions and pathways involving dibenzo-p-dioxin, a substance composed of two benzene rings linked by two ether bonds. Dibenzo-p-dioxins are generated as by-products in the manufacturing of herbicides, insecticides, fungicides, paper pulp bleaching, and in incineration, and can accumulate in milk and throughout the food chain, creating significant health concern.
intracellular calcium ion homeostasis A homeostatic process involved in the maintenance of a steady state level of calcium ions within a cell.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
negative regulation of glucose transmembrane transport Any process that decreases the frequency, rate or extent of glucose transport across a membrane. Glucose transport is the directed movement of the hexose monosaccharide glucose into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
negative regulation of insulin receptor signaling pathway Any process that stops, prevents, or reduces the frequency, rate or extent of insulin receptor signaling.
phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway A phospholipase C-activating G protein-coupled receptor signaling pathway initiated by acetylcholine binding to its receptor on the surface of a target cell, and ending with the regulation of a downstream cellular process, e.g. transcription.
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
positive regulation of angiogenesis Any process that activates or increases angiogenesis.
positive regulation of B cell receptor signaling pathway Any process that activates or increases the frequency, rate or extent of signaling pathways initiated by the cross-linking of an antigen receptor on a B cell.
positive regulation of canonical NF-kappaB signal transduction Any process that activates or increases the frequency, rate or extent of I-kappaB kinase/NF-kappaB signaling.
positive regulation of insulin secretion Any process that activates or increases the frequency, rate or extent of the regulated release of insulin.
positive regulation of odontogenesis of dentin-containing tooth Any process that activates or increases the frequency, rate or extent of the formation and development of teeth, the hard, bony appendages that are borne on the jaws, or on other bones in the walls of the mouth or pharynx of most vertebrates.
positive regulation of vascular endothelial growth factor receptor signaling pathway Any process that activates or increases the frequency, rate or extent of vascular endothelial growth factor receptor signaling pathway activity.
post-translational protein modification The process of covalently altering one or more amino acids in a protein after the protein has been completely translated and released from the ribosome.
presynaptic modulation of chemical synaptic transmission Any process, acting in the presynapse that results in modulation of chemical synaptic transmission.
regulation of dopamine secretion Any process that modulates the frequency, rate or extent of the regulated release of dopamine.
regulation of glucose transmembrane transport Any process that modulates the frequency, rate or extent of glucose transport across a membrane. Glucose transport is the directed movement of the hexose monosaccharide glucose into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
regulation of growth Any process that modulates the frequency, rate or extent of the growth of all or part of an organism so that it occurs at its proper speed, either globally or in a specific part of the organism's development.
regulation of synaptic vesicle exocytosis Any process that modulates the frequency, rate or extent of synaptic vesicle exocytosis.
regulation of transcription by RNA polymerase II Any process that modulates the frequency, rate or extent of transcription mediated by RNA polymerase II.
response to ethanol Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an ethanol stimulus.
response to glucose Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a glucose stimulus.
response to vitamin D Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a vitamin D stimulus.
response to xenobiotic stimulus Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus from a xenobiotic, a compound foreign to the organim exposed to it. It may be synthesized by another organism (like ampicilin) or it can be a synthetic chemical.

28 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P24583 PKC1 Protein kinase C-like 1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SS
P04409 PRKCA Protein kinase C alpha type Bos taurus (Bovine) EV SS
P05128 PRKCG Protein kinase C gamma type Bos taurus (Bovine) SS
P05126 PRKCB Protein kinase C beta type Bos taurus (Bovine) SS
P13677 inaC Protein kinase C, eye isozyme Drosophila melanogaster (Fruit fly) SS
P05130 Pkc53E Protein kinase C, brain isozyme Drosophila melanogaster (Fruit fly) SS
P05129 PRKCG Protein kinase C gamma type Homo sapiens (Human) SS
P17252 PRKCA Protein kinase C alpha type Homo sapiens (Human) EV
P05771 PRKCB Protein kinase C beta type Homo sapiens (Human) SS
P20444 Prkca Protein kinase C alpha type Mus musculus (Mouse) SS
P63318 Prkcg Protein kinase C gamma type Mus musculus (Mouse) SS
P68404 Prkcb Protein kinase C beta type Mus musculus (Mouse) SS
P05696 Prkca Protein kinase C alpha type Rattus norvegicus (Rat) SS
P63319 Prkcg Protein kinase C gamma type Rattus norvegicus (Rat) SS
O55173 Pdpk1 3-phosphoinositide-dependent protein kinase 1 Rattus norvegicus (Rat) SS
P09217 Prkcz Protein kinase C zeta type Rattus norvegicus (Rat) SS
F1M7Y5 Prkci Protein kinase C iota type Rattus norvegicus (Rat) SS
Q63433 Pkn1 Serine/threonine-protein kinase N1 Rattus norvegicus (Rat) SS
O08874 Pkn2 Serine/threonine-protein kinase N2 Rattus norvegicus (Rat) SS
Q64617 Prkch Protein kinase C eta type Rattus norvegicus (Rat) PR
P09216 Prkce Protein kinase C epsilon type Rattus norvegicus (Rat) PR
P09215 Prkcd Protein kinase C delta type Rattus norvegicus (Rat) PR
P47197 Akt2 RAC-beta serine/threonine-protein kinase Rattus norvegicus (Rat) SS
P47196 Akt1 RAC-alpha serine/threonine-protein kinase Rattus norvegicus (Rat) PR
Q63484 Akt3 RAC-gamma serine/threonine-protein kinase Rattus norvegicus (Rat) PR
P90980 pkc-2 Protein kinase C-like 2 Caenorhabditis elegans SS
A8KBH6 prkcb Protein kinase C beta type Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) SS
Q7SY24 prkcbb Protein kinase C beta type Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MADPAAGPPP SEGEESTVRF ARKGALRQKN VHEVKNHKFT ARFFKQPTFC SHCTDFIWGF
70 80 90 100 110 120
GKQGFQCQVC CFVVHKRCHE FVTFSCPGAD KGPASDDPRS KHKFKIHTYS SPTFCDHCGS
130 140 150 160 170 180
LLYGLIHQGM KCDTCMMNVH KRCVMNVPSL CGTDHTERRG RIYIQAHIDR EVLIVVVRDA
190 200 210 220 230 240
KNLVPMDPNG LSDPYVKLKL IPDPKSESKQ KTKTIKCSLN PEWNETFRFQ LKESDKDRRL
250 260 270 280 290 300
SVEIWDWDLT SRNDFMGSLS FGISELQKAG VDGWFKLLSQ EEGEYFNVPV PPEGSEGNEE
310 320 330 340 350 360
LRQKFERAKI GQGTKAPEEK TANTISKFDN NGNRDRMKLT DFNFLMVLGK GSFGKVMLSE
370 380 390 400 410 420
RKGTDELYAV KILKKDVVIQ DDDVECTMVE KRVLALPGKP PFLTQLHSCF QTMDRLYFVM
430 440 450 460 470 480
EYVNGGDLMY HIQQVGRFKE PHAVFYAAEI AIGLFFLQSK GIIYRDLKLD NVMLDSEGHI
490 500 510 520 530 540
KIADFGMCKE NIWDGVTTKT FCGTPDYIAP EIIAYQPYGK SVDWWAFGVL LYEMLAGQAP
550 560 570 580 590 600
FEGEDEDELF QSIMEHNVAY PKSMSKEAVA ICKGLMTKHP GKRLGCGPEG ERDIKEHAFF
610 620 630 640 650 660
RYIDWEKLER KEIQPPYKPK ARDKRDTSNF DKEFTRQPVE LTPTDKLFIM NLDQNEFAGF
670
SYTNPEFVIN V