P62205

SS
Gene name
plk1
Protein name
Serine/threonine-protein kinase PLK1
Names
EC 2.7.11.21 , Plx1 , Polo-like kinase 1 , PLK-1
Species
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
KEGG Pathway
xtr:407937
EC number
2.7.11.21: Protein-serine/threonine kinases
Protein Class

Descriptions

Polo-like kinase Plk1 acts at various stages in the cell cycle, specifically mitosis and cytokinesis. The kinase activity is regulated by a conserved C-terminal domain, termed the polo box domain (PBD). PBD is composed of the two polo boxes and an N-terminal extension, and each polo box contains a continuous six-stranded antiparallel β-sheet and an α-helix. The C-terminal region containing a polo-box domain is involved in autoinhibition via intramolecular interaction with the kinase domain in PLK1.

Autoinhibitory domains (AIDs)

361-598 (Polo box domain) SS

Target domain

44-296 (Protein kinase domain)

Relief mechanism

PTM

Assay

Accessory elements

184-207 (Activation loop from InterPro)

Target domain

36-300 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for P62205

Entry ID Method Resolution Chain Position Source
AF-P62205-F1 Predicted AlphaFoldDB

No variants for P62205

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P62205

No associated diseases with P62205

8 regional properties for P62205

Type Name Position InterPro Accession
domain Protein kinase domain 44 - 296 IPR000719
domain POLO box domain 404 - 482 IPR000959-1
domain POLO box domain 504 - 586 IPR000959-2
active_site Serine/threonine-protein kinase, active site 163 - 175 IPR008271
binding_site Protein kinase, ATP binding site 50 - 73 IPR017441
domain Second polo-box domain 503 - 584 IPR033695
domain First polo-box domain 401 - 488 IPR033701
domain Polo-like kinase 1, catalytic domain 36 - 300 IPR033702

Functions

Description
EC Number 2.7.11.21 Protein-serine/threonine kinases
Subcellular Localization
  • Nucleus
  • Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
  • Cytoplasm, cytoskeleton, spindle
  • Midbody
  • Localized at centrosomes at prophase, spindles at metaphase, and at the midbody during cytokinesis
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

6 GO annotations of cellular component

Name Definition
centrosome A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
kinetochore A multisubunit complex that is located at the centromeric region of DNA and provides an attachment point for the spindle microtubules.
midbody A thin cytoplasmic bridge formed between daughter cells at the end of cytokinesis. The midbody forms where the contractile ring constricts, and may persist for some time before finally breaking to complete cytokinesis.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
spindle pole Either of the ends of a spindle, where spindle microtubules are organized; usually contains a microtubule organizing center and accessory molecules, spindle microtubules and astral microtubules.

3 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions

7 GO annotations of biological process

Name Definition
cell division The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells.
mitotic cell cycle Progression through the phases of the mitotic cell cycle, the most common eukaryotic cell cycle, which canonically comprises four successive phases called G1, S, G2, and M and includes replication of the genome and the subsequent segregation of chromosomes into daughter cells. In some variant cell cycles nuclear replication or nuclear division may not be followed by cell division, or G1 and G2 phases may be absent.
mitotic G2 DNA damage checkpoint signaling A mitotic cell cycle checkpoint that detects and negatively regulates progression through the G2/M transition of the cell cycle in response to DNA damage.
mitotic spindle organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the microtubule spindle during a mitotic cell cycle.
negative regulation of double-strand break repair via homologous recombination Any process that stops, prevents, or reduces the frequency, rate or extent of double-strand break repair via homologous recombination.
protein localization to chromatin Any process in which a protein is transported to, or maintained at, a part of a chromosome that is organized into chromatin.
protein phosphorylation The process of introducing a phosphate group on to a protein.

9 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q2TA25 PLK1 Serine/threonine-protein kinase PLK1 Bos taurus (Bovine) SS
O97143 SAK Serine/threonine-protein kinase PLK4 Drosophila melanogaster (Fruit fly) EV
P52304 polo Serine/threonine-protein kinase polo Drosophila melanogaster (Fruit fly) SS
P53350 PLK1 Serine/threonine-protein kinase PLK1 Homo sapiens (Human) EV
Q07832 Plk1 Serine/threonine-protein kinase PLK1 Mus musculus (Mouse) EV
Q62673 Plk1 Serine/threonine-protein kinase PLK1 Rattus norvegicus (Rat) SS
Q20845 plk-3 Serine/threonine-protein kinase plk-3 Caenorhabditis elegans PR
Q9N2L7 plk-2 Serine/threonine-protein kinase plk-2 Caenorhabditis elegans SS
P34331 plk-1 Serine/threonine-protein kinase plk-1 Caenorhabditis elegans SS
10 20 30 40 50 60
MAQVAGKKLT VAPEAGKPPG IPGSSSSVKE IPEILVDPRT RRRYLRGRFL GKGGFAKCYE
70 80 90 100 110 120
ITDLESREVF AGKIVPKTML LKPHQKDKMT MEIAIQRSLD HRHVVGFHGF FEDNDFVYVV
130 140 150 160 170 180
LELCRRRSLL ELHKRRKAVT EPEARYYLKQ TILGCQYLHS NRVIHRDLKL GNLFLNDEME
190 200 210 220 230 240
VKIGDFGLAT KVEYDGERKK TLCGTPNYIA PEVLGKKGHS FEVDIWSIGC IMYTLLVGKP
250 260 270 280 290 300
PFETSCLKET YMRIKKNEYS VPKHINPMAT ALIQKMLRSE PTSRPTIDDL LNDEFFTTGY
310 320 330 340 350 360
IPSRLPTTCL TVPPRFSIAP STIDQSLRKP LTAINKGQDS PLVEKQSVPA KEEEMQQPES
370 380 390 400 410 420
TEPADCYLSE MLQQLTCVNA VKPSERAVIR QEEAEDPASV PIFWVSKWVD YSDKYGLGYQ
430 440 450 460 470 480
LCDNSVGVLF NDSTRLIMYN DGDSLQYIER NNSESYLNVR SYPAALTKKI TLLKYFRNYM
490 500 510 520 530 540
SEHLLKAGAN ITPREGDELA RLPFLRTWFR TRSAIILHLS NGTVQINFFQ DHTKIILCPL
550 560 570 580 590
MAAVSYIDEK REFHTYKLSL IQEFGCCKEL ASRLRYARTM VEKLQSSKSG VAHVKASA