AiPD: Autoinhibited Protein Database

P61586

Gene name	RHOA
Protein name	Transforming protein RhoA
Names	Rho cDNA clone 12, h12
Species	Homo sapiens (Human)
KEGG Pathway	hsa:387
EC number	3.6.5.2: Acting on GTP; involved in cellular and subcellular movement
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

91-193 (Predicted disordered autoinhibitory region) PR

Target domain
Relief mechanism
Assay	cis-regPred

AID

91-193 (Predicted disordered autoinhibitory region)

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Yeon JH et al. (2016) "Systems-wide Identification of cis-Regulatory Elements in Proteins", Cell Systems, 2, 89-100

Autoinhibited structure

Activated structure

114 structures for P61586

Entry ID	Method	Resolution	Chain	Position	Source
1A2B	X-ray	240 A	A	1-181	PDB
1CC0	X-ray	500 A	A/C	1-190	PDB
1CXZ	X-ray	220 A	A	1-181	PDB
1DPF	X-ray	200 A	A	1-180	PDB
1FTN	X-ray	210 A	A	1-193	PDB
1KMQ	X-ray	155 A	A	4-181	PDB
1LB1	X-ray	281 A	B/D/F/H	1-190	PDB
1OW3	X-ray	180 A	B	1-193	PDB
1S1C	X-ray	260 A	A/B	1-181	PDB
1TX4	X-ray	165 A	B	3-179	PDB
1X86	X-ray	322 A	B/D/F/H	1-193	PDB
1XCG	X-ray	250 A	B/F	3-180	PDB
2RGN	X-ray	350 A	C/F	1-193	PDB
3KZ1	X-ray	270 A	E/F	1-181	PDB
3LW8	X-ray	185 A	A/B/C/D	2-181	PDB
3LWN	X-ray	228 A	A/B	2-181	PDB
3LXR	X-ray	168 A	A	2-181	PDB
3MSX	X-ray	165 A	A	1-180	PDB
3T06	X-ray	284 A	B/F	3-180	PDB
4D0N	X-ray	210 A	A	1-184	PDB
4XH9	X-ray	200 A	B/E	2-180	PDB
4XOI	X-ray	209 A	A/C	1-180	PDB
4XSG	X-ray	180 A	A	1-179	PDB
4XSH	X-ray	250 A	A	1-179	PDB
5A0F	X-ray	200 A	A	1-181	PDB
5BWM	X-ray	250 A	A	1-179	PDB
5C2K	X-ray	142 A	A	1-193	PDB
5C4M	X-ray	130 A	A	1-193	PDB
5EZ6	X-ray	180 A	B	1-181	PDB
5FR1	X-ray	275 A	A	1-193	PDB
5FR2	X-ray	335 A	A	1-193	PDB
5HPY	X-ray	240 A	B/F	3-181	PDB
5IRC	X-ray	172 A	D/F	2-181	PDB
5JCP	X-ray	210 A	A/B	1-181	PDB
5JHG	X-ray	250 A	B/F	1-181	PDB
5JHH	X-ray	230 A	B/F	1-181	PDB
5M6X	X-ray	240 A	B/I	2-193	PDB
5M70	X-ray	220 A	B/G	2-193	PDB
5ZHX	X-ray	350 A	e/f/g/h	1-193	PDB
6BC0	X-ray	220 A	F	1-181	PDB
6BCA	X-ray	200 A	C/F	1-181	PDB
6BCB	X-ray	140 A	F	1-181	PDB
6KX2	X-ray	145 A	A	1-181	PDB
6KX3	X-ray	198 A	A	1-181	PDB
6R3V	X-ray	175 A	B	1-193	PDB
6V6M	X-ray	139 A	A	1-181	PDB
6V6U	X-ray	116 A	A	1-181	PDB
6V6V	X-ray	140 A	A	1-181	PDB
7G80	X-ray	167 A	A	1-184	PDB
7G81	X-ray	151 A	A	1-184	PDB
7G82	X-ray	141 A	A	1-184	PDB
7G83	X-ray	131 A	A	1-184	PDB
7G84	X-ray	181 A	A	1-184	PDB
7G85	X-ray	174 A	A	1-184	PDB
7G86	X-ray	170 A	A	1-184	PDB
7G87	X-ray	205 A	A	1-184	PDB
7G88	X-ray	187 A	A	1-184	PDB
7G89	X-ray	190 A	A	1-184	PDB
7G8A	X-ray	150 A	A	1-184	PDB
7G8B	X-ray	142 A	A	1-184	PDB
7G8C	X-ray	218 A	A	1-184	PDB
7G8D	X-ray	194 A	A	1-184	PDB
7G8E	X-ray	179 A	A	1-184	PDB
7G8F	X-ray	142 A	A	1-184	PDB
7G8G	X-ray	192 A	A	1-184	PDB
7G8H	X-ray	167 A	A	1-184	PDB
7G8I	X-ray	247 A	A	1-184	PDB
7G8J	X-ray	199 A	A	1-184	PDB
7G8K	X-ray	149 A	A	1-184	PDB
7G8L	X-ray	160 A	A	1-184	PDB
7G8M	X-ray	203 A	A	1-184	PDB
7G8N	X-ray	232 A	A	1-184	PDB
7G8O	X-ray	158 A	A	1-184	PDB
7G8P	X-ray	221 A	A	1-184	PDB
7G8Q	X-ray	156 A	A	1-184	PDB
7G8R	X-ray	144 A	A	1-184	PDB
7G8S	X-ray	160 A	A	1-184	PDB
7G8T	X-ray	139 A	A	1-184	PDB
7G8U	X-ray	244 A	A	1-184	PDB
7G8V	X-ray	145 A	A	1-184	PDB
7G8W	X-ray	194 A	A	1-184	PDB
7G8X	X-ray	171 A	A	1-184	PDB
7G8Y	X-ray	175 A	A	1-184	PDB
7G8Z	X-ray	151 A	A	1-184	PDB
7G90	X-ray	191 A	A	1-184	PDB
7G91	X-ray	229 A	A	1-184	PDB
7G92	X-ray	187 A	A	1-184	PDB
7G93	X-ray	169 A	A	1-184	PDB
7G94	X-ray	147 A	A	1-184	PDB
7G95	X-ray	155 A	A	1-184	PDB
7G96	X-ray	230 A	A	1-184	PDB
7G97	X-ray	230 A	A	1-184	PDB
7G98	X-ray	288 A	A	1-184	PDB
7G99	X-ray	178 A	A	1-184	PDB
7G9A	X-ray	258 A	A	1-184	PDB
7G9B	X-ray	255 A	A	1-184	PDB
7G9C	X-ray	269 A	A	1-184	PDB
7G9D	X-ray	266 A	A	1-184	PDB
7G9E	X-ray	215 A	A	1-184	PDB
7G9F	X-ray	194 A	A	1-184	PDB
7G9G	X-ray	208 A	A	1-184	PDB
7G9H	X-ray	275 A	A	1-184	PDB
7G9I	X-ray	220 A	A	1-184	PDB
7G9J	X-ray	197 A	A	1-184	PDB
7QSC	X-ray	191 A	B/D	2-193	PDB
7QTM	X-ray	225 A	B/I	2-193	PDB
7WZA	X-ray	150 A	A	1-181	PDB
7WZC	X-ray	180 A	A	1-181	PDB
8BNT	X-ray	140 A	A	1-184	PDB
8FC7	EM	330 A	E/F/G/H	1-193	PDB
8FC9	EM	375 A	E/F/G/H	1-193	PDB
8FCB	EM	352 A	E/F/G/H	1-193	PDB
8T1C	EM	349 A	E	1-193	PDB
AF-P61586-F1	Predicted				AlphaFoldDB

45 variants for P61586

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
COSM3940568 RCV000432532 CA16602950 rs1057519953 RCV000444013 RCV000425080 RCV000443944 RCV000431457	5	R>L	Non-Hodgkin lymphoma Carcinoma of esophagus oesophagus Gastric adenocarcinoma Neoplasm of the large intestine Breast neoplasm [ClinVar, Cosmic]	Yes	ClinGen cosmic curated ClinVar Ensembl dbSNP
RCV000432727 RCV000421840 CA16602949 RCV000422905 RCV000444856 rs1057519953 COSM190569 RCV000440078	5	R>Q	Non-Hodgkin lymphoma Carcinoma of esophagus large_intestine Variant assessed as Somatic; impact. Gastric adenocarcinoma Neoplasm of the large intestine haematopoietic_and_lymphoid_tissue Breast neoplasm [ClinVar, Cosmic, NCI-TCGA]	Yes	ClinGen cosmic curated ClinVar Ensembl NCI-TCGA dbSNP
RCV000430318 RCV000423113 RCV000430517 CA16602948 RCV000420653 RCV000441218 COSM446704 rs1057519952	5	R>W	Non-Hodgkin lymphoma Carcinoma of esophagus oesophagus Variant assessed as Somatic; impact. Gastric adenocarcinoma Neoplasm of the large intestine breast Breast neoplasm [ClinVar, Cosmic, NCI-TCGA]	Yes	ClinGen cosmic curated ClinVar Ensembl NCI-TCGA dbSNP
RCV000435482 RCV000435238 RCV000418248 RCV000428054 CA16602947 rs1057519951	40	E>K	Squamous cell carcinoma of the head and neck Variant assessed as Somatic; impact. Gastric adenocarcinoma Lung adenocarcinoma Breast neoplasm [ClinVar, NCI-TCGA]	Yes	ClinGen ClinVar Ensembl NCI-TCGA dbSNP
RCV000425677 RCV000425897 COSM125851 CA16602946 rs1057519951 RCV000443212 RCV000436482	40	E>Q	Squamous cell carcinoma of the head and neck upper_aerodigestive_tract Variant assessed as Somatic; impact. Gastric adenocarcinoma Lung adenocarcinoma breast Breast neoplasm [ClinVar, Cosmic, NCI-TCGA]	Yes	ClinGen cosmic curated ClinVar Ensembl NCI-TCGA dbSNP
CA16602951 RCV000420783 rs1057519954 RCV000438221 RCV000427520	42	Y>C	Non-Hodgkin lymphoma Squamous cell carcinoma of the head and neck Variant assessed as Somatic; impact. Gastric adenocarcinoma [ClinVar, NCI-TCGA]	Yes	ClinGen ClinVar Ensembl NCI-TCGA dbSNP
CA16602953 RCV000418559 COSM3357717 RCV000430340 rs1057519954 RCV000440615	42	Y>F	Non-Hodgkin lymphoma Squamous cell carcinoma of the head and neck Gastric adenocarcinoma haematopoietic_and_lymphoid_tissue [ClinVar, Cosmic]	Yes	ClinGen cosmic curated ClinVar Ensembl dbSNP
RCV000419217 CA16602952 RCV000427521 RCV000436909 rs1057519954	42	Y>S	Non-Hodgkin lymphoma Squamous cell carcinoma of the head and neck Variant assessed as Somatic; impact. Gastric adenocarcinoma [ClinVar, NCI-TCGA]	Yes	ClinGen ClinVar Ensembl NCI-TCGA dbSNP
VAR_083545 rs1575653629 RCV001526536 RCV001095368 RCV001539108 CA352788014 RCV001526531	47	E>K	Ectodermal dysplasia with facial dysmorphism and acral, ocular, and brain anomalies Hemihypertrophy neuro-ectodermal phenotype EDFAOB; somatic mosaic variant; decreased Rho protein signal transduction; decreased substrate adhesion-dependent cell spreading; decreased number of stress fibers assembly; decreased cytoplasmic microtubule organization [ClinVar, UniProt]	Yes	ClinGen ClinVar Ensembl dbSNP UniProt
rs1553631976 RCV000623047 CA352786786	66	Y>H	Inborn genetic diseases [ClinVar]	Yes	ClinGen ClinVar Ensembl dbSNP
RCV002221594 VAR_083546 rs1575647025 CA352786720 TCGA novel	71	P>S	Ectodermal dysplasia with facial dysmorphism and acral, ocular, and brain anomalies Variant assessed as Somatic; impact. EDFAOB; somatic mosaic variant; decreased Rho protein signal transduction; decreased substrate adhesion-dependent cell spreading; decreased stress fibers assembly; decreased cytoplasmic microtubule organization [ClinVar, NCI-TCGA, UniProt]	Yes	ClinGen ClinVar NCI-TCGA Ensembl dbSNP UniProt
rs1386884613 CA352788306	3	A>G		No	ClinGen gnomAD
TCGA novel	3	A>T	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA352788269 rs1575653732	9	V>G		No	ClinGen Ensembl
CA74517701 COSM3824157 rs11552761	17	G>A	Variant assessed as Somatic; impact. breast [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated Ensembl NCI-TCGA
CA74517702 rs11552761	17	G>E	Variant assessed as Somatic; impact. [NCI-TCGA]	No	ClinGen Ensembl NCI-TCGA
TCGA novel	22	L>F	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA74517671 rs11552762	32	E>D		No	ClinGen Ensembl
TCGA novel	32	E>G	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
CA352787998 rs1259336198	49	D>G		No	ClinGen gnomAD
rs1575647051 RCV001029738 CA352786855	61	A>T		No	ClinGen ClinVar Ensembl dbSNP
TCGA novel	67	D>N	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
COSM3736658 rs866298036 CA74513482	75	P>S	Variant assessed as Somatic; impact. skin [NCI-TCGA, Cosmic]	No	ClinGen cosmic curated Ensembl NCI-TCGA
rs372868120 CA352786644	78	D>E		No	ClinGen ESP ExAC TOPMed gnomAD
TCGA novel	78	D>N	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs1381401434 CA352786588	86	I>N		No	ClinGen Ensembl
rs1386276711 CA352786592	86	I>V		No	ClinGen gnomAD
CA74513411 rs1019221398	87	D>N	Variant assessed as Somatic; 0.0 impact. [NCI-TCGA]	No	ClinGen NCI-TCGA TOPMed gnomAD
rs866333389 CA74508739	108	P>L	Variant assessed as Somatic; impact. [NCI-TCGA]	No	ClinGen Ensembl NCI-TCGA
rs1260716785 CA352785839	114	L>V		No	ClinGen gnomAD
CA352785703 rs1200080340	129	R>Q		No	ClinGen TOPMed
rs1420305556 CA352785678	132	A>V		No	ClinGen TOPMed
CA2397838 rs779873892	134	M>L		No	ClinGen ExAC TOPMed gnomAD
rs371460390 CA74508707	134	M>T		No	ClinGen ESP TOPMed gnomAD
rs1376671049 CA352784450	137	E>*		No	ClinGen gnomAD
rs773583237 CA352784445	137	E>D		No	ClinGen ExAC gnomAD
rs775207141 CA2397788	141	P>S		No	ClinGen ExAC gnomAD
TCGA novel	143	E>A	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs745317533 CA2397786	146	D>E		No	ClinGen ExAC TOPMed gnomAD
rs1357783668 CA352784369	147	M>I		No	ClinGen TOPMed
CA352784370 rs1260250679	147	M>R		No	ClinGen gnomAD
CA352784342 rs1465894043	151	I>T		No	ClinGen TOPMed
CA352784336 rs1250486455 COSM1328154	152	G>C	ovary Variant assessed as Somatic; 0.0 impact. [Cosmic, NCI-TCGA]	No	ClinGen cosmic curated NCI-TCGA gnomAD
TCGA novel	153	A>T	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA
rs11552757	161	A>V	Variant assessed as Somatic; impact. [NCI-TCGA]	No	NCI-TCGA

1 associated diseases with P61586

[MIM: 618727]: Ectodermal dysplasia with facial dysmorphism and acral, ocular, and brain anomalies (EDFAOB)

A neuroectodermal syndrome characterized by linear hypopigmentation, alopecia, apparently asymptomatic leukoencephalopathy, and facial, ocular, dental and acral anomalies. Patients show no intellectual or neurologic impairment. {ECO:0000269|PubMed:31570889}. Note=The disease is caused by variants affecting the gene represented in this entry.

Without disease ID

A neuroectodermal syndrome characterized by linear hypopigmentation, alopecia, apparently asymptomatic leukoencephalopathy, and facial, ocular, dental and acral anomalies. Patients show no intellectual or neurologic impairment. {ECO:0000269|PubMed:31570889}. Note=The disease is caused by variants affecting the gene represented in this entry.

1 regional properties for P61586

Type	Name	Position	InterPro Accession
domain	Small GTP-binding protein domain	5 - 159	IPR005225

Functions

		Description
EC Number	3.6.5.2	Acting on GTP; involved in cellular and subcellular movement
Subcellular Localization	Cell membrane; Lipid-anchor; Cytoplasmic side Cytoplasm, cytoskeleton Cleavage furrow Cytoplasm, cell cortex Midbody Cell projection, lamellipodium Cell projection, dendrite Nucleus Cytoplasm	Localized to cell-cell contacts in calcium-treated keratinocytes (By similarity) Translocates to the equatorial region before furrow formation in a ECT2-dependent manner Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in an activated form and in a myosin- and actin-independent manner
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

26 GO annotations of cellular component

Name	Definition
apical junction complex	A functional unit located near the cell apex at the points of contact between epithelial cells, which in vertebrates is composed of the tight junction, the zonula adherens, and desmosomes and in some invertebrates, such as Drosophila, is composed of the subapical complex (SAC), the zonula adherens and the septate junction. Functions in the regulation of cell polarity, tissue integrity and intercellular adhesion and permeability.
axon	The long process of a neuron that conducts nerve impulses, usually away from the cell body to the terminals and varicosities, which are sites of storage and release of neurotransmitter.
cell cortex	The region of a cell that lies just beneath the plasma membrane and often, but not always, contains a network of actin filaments and associated proteins.
cell junction	A cellular component that forms a specialized region of connection between two or more cells, or between a cell and the extracellular matrix, or between two membrane-bound components of a cell, such as flagella.
cell periphery	The part of a cell encompassing the cell cortex, the plasma membrane, and any external encapsulating structures.
cell projection	A prolongation or process extending from a cell, e.g. a flagellum or axon.
cleavage furrow	The cleavage furrow is a plasma membrane invagination at the cell division site. The cleavage furrow begins as a shallow groove and eventually deepens to divide the cytoplasm.
cytoplasmic vesicle	A vesicle found in the cytoplasm of a cell.
cytoskeleton	A cellular structure that forms the internal framework of eukaryotic and prokaryotic cells. The cytoskeleton includes intermediate filaments, microfilaments, microtubules, the microtrabecular lattice, and other structures characterized by a polymeric filamentous nature and long-range order within the cell. The various elements of the cytoskeleton not only serve in the maintenance of cellular shape but also have roles in other cellular functions, including cellular movement, cell division, endocytosis, and movement of organelles.
cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
dendritic spine	A small, membranous protrusion from a dendrite that forms a postsynaptic compartment, typically receiving input from a single presynapse. They function as partially isolated biochemical and an electrical compartments. Spine morphology is variable:they can be thin, stubby, mushroom, or branched, with a continuum of intermediate morphologies. They typically terminate in a bulb shape, linked to the dendritic shaft by a restriction. Spine remodeling is though to be involved in synaptic plasticity.
endoplasmic reticulum membrane	The lipid bilayer surrounding the endoplasmic reticulum.
endosome	A vacuole to which materials ingested by endocytosis are delivered.
extracellular exosome	A vesicle that is released into the extracellular region by fusion of the limiting endosomal membrane of a multivesicular body with the plasma membrane. Extracellular exosomes, also simply called exosomes, have a diameter of about 40-100 nm.
extrinsic component of cytoplasmic side of plasma membrane	The component of a plasma membrane consisting of gene products and protein complexes that are loosely bound to its cytoplasmic surface, but not integrated into the hydrophobic region.
ficolin-1-rich granule membrane	The lipid bilayer surrounding a ficolin-1-rich granule.
focal adhesion	A cell-substrate junction that anchors the cell to the extracellular matrix and that forms a point of termination of actin filaments. In insects focal adhesion has also been referred to as hemi-adherens junction (HAJ).
glutamatergic synapse	A synapse that uses glutamate as a neurotransmitter.
lamellipodium	A thin sheetlike process extended by the leading edge of a migrating cell or extending cell process; contains a dense meshwork of actin filaments.
midbody	A thin cytoplasmic bridge formed between daughter cells at the end of cytokinesis. The midbody forms where the contractile ring constricts, and may persist for some time before finally breaking to complete cytokinesis.
nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
plasma membrane	The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
postsynapse	The part of a synapse that is part of the post-synaptic cell.
ruffle membrane	The portion of the plasma membrane surrounding a ruffle.
secretory granule membrane	The lipid bilayer surrounding a secretory granule.
vesicle	Any small, fluid-filled, spherical organelle enclosed by membrane.

8 GO annotations of molecular function

Name	Definition
G protein activity	A molecular function regulator that cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular processes. Intrinsic GTPase activity returns the G protein to its GDP-bound state. The return to the GDP-bound state can be accelerated by the action of a GTPase-activating protein (GAP).
GDP binding	Binding to GDP, guanosine 5'-diphosphate.
GTP binding	Binding to GTP, guanosine triphosphate.
GTPase activity	Catalysis of the reaction: GTP + H2O = GDP + H+ + phosphate.
myosin binding	Binding to a myosin; myosins are any of a superfamily of molecular motor proteins that bind to actin and use the energy of ATP hydrolysis to generate force and movement along actin filaments.
protein domain specific binding	Binding to a specific domain of a protein.
protein kinase binding	Binding to a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate.
Rho GDP-dissociation inhibitor binding	Binding to a Rho GDP-dissociation inhibitor protein.

93 GO annotations of biological process

Name	Definition
actin cytoskeleton organization	A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising actin filaments and their associated proteins.
actin cytoskeleton reorganization	A process that is carried out at the cellular level which results in dynamic structural changes to the arrangement of constituent parts of cytoskeletal structures comprising actin filaments and their associated proteins.
actin filament organization	A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising actin filaments. Includes processes that control the spatial distribution of actin filaments, such as organizing filaments into meshworks, bundles, or other structures, as by cross-linking.
alpha-beta T cell lineage commitment	The process in which a pro-T cell becomes committed to becoming an alpha-beta T cell.
androgen receptor signaling pathway	The series of molecular signals initiated by androgen binding to its receptor, and ending with the regulation of a downstream cellular process, e.g. transcription.
angiotensin-mediated vasoconstriction involved in regulation of systemic arterial blood pressure	The decrease in blood vessel diameter as a result of the release of angiotensin into the blood stream.
apical junction assembly	The formation of an apical junction, a functional unit located near the cell apex at the points of contact between epithelial cells composed of the tight junction, the zonula adherens junction and the desmosomes, by the aggregation, arrangement and bonding together of its constituents.
apolipoprotein A-I-mediated signaling pathway	The series of molecular signals initiated by apolipoprotein A-I binding to its receptor on the surface of a target cell, and ending with the regulation of a downstream cellular process, e.g. transcription.
beta selection	The process in which successful recombination of a T cell receptor beta chain into a translatable protein coding sequence leads to rescue from apoptosis and subsequent proliferation of an immature T cell.
cell junction assembly	A cellular process that results in the aggregation, arrangement and bonding together of a set of components to form a cell junction.
cell migration	The controlled self-propelled movement of a cell from one site to a destination guided by molecular cues. Cell migration is a central process in the development and maintenance of multicellular organisms.
cell-matrix adhesion	The binding of a cell to the extracellular matrix via adhesion molecules.
cellular response to chemokine	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a chemokine stimulus.
cellular response to cytokine stimulus	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cytokine stimulus.
cellular response to lipopolysaccharide	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a lipopolysaccharide stimulus; lipopolysaccharide is a major component of the cell wall of gram-negative bacteria.
cerebral cortex cell migration	The orderly movement of cells from one site to another in the cerebral cortex.
cleavage furrow formation	Generation of the cleavage furrow, a shallow groove in the cell surface near the old metaphase plate that marks the site of cytokinesis. This process includes the recruitment and localized activation of signals such as RhoA at the site of the future furrow to ensure that furrowing initiates at the correct site in the cell.
cortical cytoskeleton organization	A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures in the cell cortex, i.e. just beneath the plasma membrane.
cytoplasmic microtubule organization	A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of structures formed of microtubules and associated proteins in the cytoplasm of a cell.
endothelial cell migration	The orderly movement of an endothelial cell into the extracellular matrix to form an endothelium.
endothelial tube lumen extension	Any endothelial tube morphogenesis process by which the tube is increased in length.
establishment of epithelial cell apical/basal polarity	The specification and formation of the apicobasal polarity of an epithelial cell.
establishment or maintenance of cell polarity	Any cellular process that results in the specification, formation or maintenance of anisotropic intracellular organization or cell growth patterns.
forebrain radial glial cell differentiation	The process in which neuroepithelial cells of the neural tube give rise to radial glial cells, specialized bipotential progenitors cells of the forebrain. Differentiation includes the processes involved in commitment of a cell to a specific fate.
GTP metabolic process	The chemical reactions and pathways involving GTP, guanosine triphosphate.
kidney development	The process whose specific outcome is the progression of the kidney over time, from its formation to the mature structure. The kidney is an organ that filters the blood and/or excretes the end products of body metabolism in the form of urine.
mitotic cleavage furrow formation	Any cleavage furrow formation that is involved in mitotic cell cycle.
mitotic spindle assembly	Mitotic bipolar spindle assembly begins with spindle microtubule nucleation from the separated spindle pole body, includes spindle elongation during prometaphase, and is complete when all kinetochores are stably attached the spindle, and the spindle assembly checkpoint is satisfied.
motor neuron apoptotic process	Any apoptotic process in a motor neuron, an efferent neuron that passes from the central nervous system or a ganglion toward or to a muscle and conducts an impulse that causes movement.
negative chemotaxis	The directed movement of a motile cell or organism towards a lower concentration of a chemical.
negative regulation of cell migration involved in sprouting angiogenesis	Any process that decreases the frequency, rate or extent of cell migration involved in sprouting angiogenesis. Cell migration involved in sprouting angiogenesis is the orderly movement of endothelial cells into the extracellular matrix in order to form new blood vessels contributing to the process of sprouting angiogenesis.
negative regulation of cell size	Any process that reduces cell size.
negative regulation of cell-substrate adhesion	Any process that decreases the frequency, rate or extent of cell-substrate adhesion. Cell-substrate adhesion is the attachment of a cell to the underlying substrate via adhesion molecules.
negative regulation of I-kappaB kinase/NF-kappaB signaling	Any process that stops, prevents, or reduces the frequency, rate or extent of -kappaB kinase/NF-kappaB signaling.
negative regulation of intracellular steroid hormone receptor signaling pathway	Any process that stops, prevents, or reduces the frequency, rate or extent of the activity of any intracellular steroid hormone receptor signaling pathway.
negative regulation of motor neuron apoptotic process	Any process that stops, prevents or reduces the frequency, rate or extent of motor neuron apoptotic process.
negative regulation of neuron differentiation	Any process that stops, prevents, or reduces the frequency, rate or extent of neuron differentiation.
negative regulation of neuron projection development	Any process that decreases the rate, frequency or extent of neuron projection development. Neuron projection development is the process whose specific outcome is the progression of a neuron projection over time, from its formation to the mature structure. A neuron projection is any process extending from a neural cell, such as axons or dendrites (collectively called neurites).
negative regulation of oxidative phosphorylation	Any process that decreases the frequency, rate or extent of the chemical reactions and pathways resulting in the phosphorylation of ADP to ATP that accompanies the oxidation of a metabolite through the operation of the respiratory chain. Oxidation of compounds establishes a proton gradient across the membrane, providing the energy for ATP synthesis.
negative regulation of reactive oxygen species biosynthetic process	Any process that stops, prevents or reduces the frequency, rate or extent of reactive oxygen species biosynthetic process.
negative regulation of vascular associated smooth muscle cell migration	Any process that stops, prevents or reduces the frequency, rate or extent of vascular associated smooth muscle cell migration.
negative regulation of vascular associated smooth muscle cell proliferation	Any process that stops, prevents or reduces the frequency, rate or extent of vascular smooth muscle cell proliferation.
neuron migration	The characteristic movement of an immature neuron from germinal zones to specific positions where they will reside as they mature.
neuron projection morphogenesis	The process in which the anatomical structures of a neuron projection are generated and organized. A neuron projection is any process extending from a neural cell, such as axons or dendrites.
odontogenesis	The process whose specific outcome is the progression of a tooth or teeth over time, from formation to the mature structure(s). A tooth is any hard bony, calcareous, or chitinous organ found in the mouth or pharynx of an animal and used in procuring or masticating food.
ossification involved in bone maturation	The formation of bone or of a bony substance, or the conversion of fibrous tissue or of cartilage into bone, involved in the progression of the skeleton from its formation to its mature state.
positive regulation of actin filament polymerization	Any process that activates or increases the frequency, rate or extent of actin polymerization.
positive regulation of alpha-beta T cell differentiation	Any process that activates or increases the frequency, rate or extent of alpha-beta T cell differentiation.
positive regulation of cell growth	Any process that activates or increases the frequency, rate, extent or direction of cell growth.
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process	Any process that activates or increases the activity of a cysteine-type endopeptidase involved in the apoptotic process.
positive regulation of cytokinesis	Any process that activates or increases the frequency, rate or extent of the division of the cytoplasm of a cell, and its separation into two daughter cells.
positive regulation of I-kappaB kinase/NF-kappaB signaling	Any process that activates or increases the frequency, rate or extent of I-kappaB kinase/NF-kappaB signaling.
positive regulation of leukocyte adhesion to vascular endothelial cell	Any process that activates or increases the frequency, rate or extent of leukocyte adhesion to vascular endothelial cell.
positive regulation of lipase activity	Any process that increases the frequency, rate or extent of lipase activity, the hydrolysis of a lipid or phospholipid.
positive regulation of neuron apoptotic process	Any process that activates or increases the frequency, rate or extent of cell death of neurons by apoptotic process.
positive regulation of neuron differentiation	Any process that activates or increases the frequency, rate or extent of neuron differentiation.
positive regulation of NIK/NF-kappaB signaling	Any process that activates or increases the frequency, rate or extent of NIK/NF-kappaB signaling.
positive regulation of podosome assembly	Any process that activates or increases the rate or extent of podosome assembly.
positive regulation of protein serine/threonine kinase activity	Any process that increases the rate, frequency, or extent of protein serine/threonine kinase activity.
positive regulation of stress fiber assembly	Any process that activates or increases the frequency, rate or extent of the assembly of a stress fiber, a bundle of microfilaments and other proteins found in fibroblasts.
positive regulation of T cell migration	Any process that activates or increases the frequency, rate or extent of T cell migration.
positive regulation of translation	Any process that activates or increases the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of proteins by the translation of mRNA or circRNA.
positive regulation of vascular associated smooth muscle contraction	Any process that activates or increases the frequency, rate or extent of vascular smooth muscle contraction.
regulation of actin cytoskeleton organization	Any process that modulates the frequency, rate or extent of the formation, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising actin filaments and their associated proteins.
regulation of calcium ion transport	Any process that modulates the frequency, rate or extent of the directed movement of calcium ions into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
regulation of cell migration	Any process that modulates the frequency, rate or extent of cell migration.
regulation of cell shape	Any process that modulates the surface configuration of a cell.
regulation of dendrite development	Any process that modulates the frequency, rate or extent of dendrite development.
regulation of focal adhesion assembly	Any process that modulates the frequency, rate or extent of focal adhesion formation, the establishment and maturation of focal adhesions.
regulation of microtubule cytoskeleton organization	Any process that modulates the frequency, rate or extent of the formation, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising microtubules and their associated proteins.
regulation of modification of postsynaptic actin cytoskeleton	Any process that modulates the frequency, rate or extent of modification of postsynaptic actin cytoskeleton.
regulation of neural precursor cell proliferation	Any process that modulates the frequency, rate or extent of neural precursor cell proliferation.
regulation of osteoblast proliferation	Any process that modulates the frequency, rate or extent of osteoblast proliferation.
regulation of systemic arterial blood pressure by endothelin	The process in which endothelin modulates the force with which blood passes through the circulatory system. Endothelin is a hormone that is released by the endothelium, and it is a vasoconstrictor.
regulation of transcription by RNA polymerase II	Any process that modulates the frequency, rate or extent of transcription mediated by RNA polymerase II.
response to amino acid	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an amino acid stimulus. An amino acid is a carboxylic acids containing one or more amino groups.
response to ethanol	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an ethanol stimulus.
response to glucocorticoid	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a glucocorticoid stimulus. Glucocorticoids are hormonal C21 corticosteroids synthesized from cholesterol with the ability to bind with the cortisol receptor and trigger similar effects. Glucocorticoids act primarily on carbohydrate and protein metabolism, and have anti-inflammatory effects.
response to glucose	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a glucose stimulus.
response to hypoxia	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating lowered oxygen tension. Hypoxia, defined as a decline in O2 levels below normoxic levels of 20.8 - 20.95%, results in metabolic adaptation at both the cellular and organismal level.
response to mechanical stimulus	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a mechanical stimulus.
response to xenobiotic stimulus	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus from a xenobiotic, a compound foreign to the organim exposed to it. It may be synthesized by another organism (like ampicilin) or it can be a synthetic chemical.
Rho protein signal transduction	The series of molecular signals within the cell that are mediated by a member of the Rho family of proteins switching to a GTP-bound active state.
Roundabout signaling pathway	The series of molecular signals initiated by a SLIT protein binding to a Roundabout (ROBO) family receptor on the surface of a target cell, and ending with the regulation of a downstream cellular process, e.g. transcription.
skeletal muscle satellite cell migration	The orderly movement of a skeletal muscle satellite cell from one site to another. Migration of these cells is a key step in the process of growth and repair of skeletal muscle cells.
skeletal muscle tissue development	The developmental sequence of events leading to the formation of adult skeletal muscle tissue. The main events are: the fusion of myoblasts to form myotubes that increase in size by further fusion to them of myoblasts, the formation of myofibrils within their cytoplasm and the establishment of functional neuromuscular junctions with motor neurons. At this stage they can be regarded as mature muscle fibers.
stress fiber assembly	The aggregation, arrangement and bonding together of a set of components to form a stress fiber. A stress fiber is a contractile actin filament bundle that consists of short actin filaments with alternating polarity.
stress-activated protein kinase signaling cascade	The series of molecular signals in which a stress-activated protein kinase (SAPK) cascade relays a signal.
substantia nigra development	The progression of the substantia nigra over time from its initial formation until its mature state. The substantia nigra is the layer of gray substance that separates the posterior parts of the cerebral peduncles (tegmentum mesencephali) from the anterior parts; it normally includes a posterior compact part with many pigmented cells (pars compacta) and an anterior reticular part whose cells contain little pigment (pars reticularis).
substrate adhesion-dependent cell spreading	The morphogenetic process that results in flattening of a cell as a consequence of its adhesion to a substrate.
trabecula morphogenesis	The process of shaping a trabecula in an organ. A trabecula is a small, often microscopic, tissue element in the form of a small beam, strut or rod, which generally has a mechanical function. Trabecula are usually but not necessarily, composed of dense collagenous tissue.
Wnt signaling pathway, planar cell polarity pathway	The series of molecular signals initiated by binding of a Wnt protein to a receptor on the surface of the target cell where activated receptors signal via downstream effectors including C-Jun N-terminal kinase (JNK) to modulate cytoskeletal elements and control cell polarity.
wound healing, spreading of cells	The migration of a cell along or through a wound gap that contributes to the reestablishment of a continuous surface.

38 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q00246	RHO4	GTP-binding protein RHO4	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	PR
Q17QI8	Rhov	Rho-related GTP-binding protein RhoV	Bos taurus (Bovine)	PR
Q2HJ68	RND1	Rho-related GTP-binding protein Rho6	Bos taurus (Bovine)	PR
Q3ZBW5	RHOB	Rho-related GTP-binding protein RhoB	Bos taurus (Bovine)	PR
P61585	RHOA	Transforming protein RhoA	Bos taurus (Bovine)	PR
Q1RMJ6	RHOC	Rho-related GTP-binding protein RhoC	Bos taurus (Bovine)	PR
Q9PSX7	RHOC	Rho-related GTP-binding protein RhoC	Gallus gallus (Chicken)	PR
P24406	RHOA	Transforming protein RhoA	Canis lupus familiaris (Dog) (Canis familiaris)	PR
P48148	Rho1	Ras-like GTP-binding protein Rho1	Drosophila melanogaster (Fruit fly)	PR
O94955	RHOBTB3	Rho-related BTB domain-containing protein 3	Homo sapiens (Human)	EV
O94844	RHOBTB1	Rho-related BTB domain-containing protein 1	Homo sapiens (Human)	PR
P08134	RHOC	Rho-related GTP-binding protein RhoC	Homo sapiens (Human)	PR
P61587	RND3	Rho-related GTP-binding protein RhoE	Homo sapiens (Human)	PR
P62745	RHOB	Rho-related GTP-binding protein RhoB	Homo sapiens (Human)	PR
Q92730	RND1	Rho-related GTP-binding protein Rho6	Homo sapiens (Human)	PR
Q96L33	RHOV	Rho-related GTP-binding protein RhoV	Homo sapiens (Human)	PR
Q8BLR7	Rnd1	Rho-related GTP-binding protein Rho6	Mus musculus (Mouse)	PR
P61588	Rnd3	Rho-related GTP-binding protein RhoE	Mus musculus (Mouse)	PR
Q9DAK3	Rhobtb1	Rho-related BTB domain-containing protein 1	Mus musculus (Mouse)	PR
Q9CTN4	Rhobtb3	Rho-related BTB domain-containing protein 3	Mus musculus (Mouse)	SS
Q8VDU1	Rhov	Rho-related GTP-binding protein RhoV	Mus musculus (Mouse)	PR
Q62159	Rhoc	Rho-related GTP-binding protein RhoC	Mus musculus (Mouse)	PR
P62746	Rhob	Rho-related GTP-binding protein RhoB	Mus musculus (Mouse)	PR
Q9QUI0	Rhoa	Transforming protein RhoA	Mus musculus (Mouse)	PR
O77683	RND3	Rho-related GTP-binding protein RhoE	Sus scrofa (Pig)	PR
Q6SA80	Rnd3	Rho-related GTP-binding protein RhoE	Rattus norvegicus (Rat)	PR
Q9Z1Y0	Rhov	Rho-related GTP-binding protein RhoV	Rattus norvegicus (Rat)	PR
P61589	Rhoa	Transforming protein RhoA	Rattus norvegicus (Rat)	PR
P62747	Rhob	Rho-related GTP-binding protein RhoB	Rattus norvegicus (Rat)	PR
Q9SSX0	RAC1	Rac-like GTP-binding protein 1	Oryza sativa subsp japonica (Rice)	PR
Q6Z808	RAC3	Rac-like GTP-binding protein 3	Oryza sativa subsp japonica (Rice)	PR
Q68Y52	RAC2	Rac-like GTP-binding protein 2	Oryza sativa subsp japonica (Rice)	PR
Q67VP4	RAC4	Rac-like GTP-binding protein 4	Oryza sativa subsp japonica (Rice)	PR
Q22038	rho-1	Ras-like GTP-binding protein rhoA	Caenorhabditis elegans	PR
Q9SU67	ARAC8	Rac-like GTP-binding protein ARAC8	Arabidopsis thaliana (Mouse-ear cress)	PR
O82480	ARAC7	Rac-like GTP-binding protein ARAC7	Arabidopsis thaliana (Mouse-ear cress)	PR
Q38903	ARAC2	Rac-like GTP-binding protein ARAC2	Arabidopsis thaliana (Mouse-ear cress)	PR
O82481	ARAC10	Rac-like GTP-binding protein ARAC10	Arabidopsis thaliana (Mouse-ear cress)	PR

10	20	30	40	50	60
MAAIRKKLVI	VGDGACGKTC	LLIVFSKDQF	PEVYVPTVFE	NYVADIEVDG	KQVELALWDT
70	80	90	100	110	120
AGQEDYDRLR	PLSYPDTDVI	LMCFSIDSPD	SLENIPEKWT	PEVKHFCPNV	PIILVGNKKD
130	140	150	160	170	180
LRNDEHTRRE	LAKMKQEPVK	PEEGRDMANR	IGAFGYMECS	AKTKDGVREV	FEMATRAALQ
190
ARRGKKKSGC	LVL