P60570
SS
Gene name |
Panx1 (Px1) |
Protein name |
Pannexin-1 [Cleaved into: Caspase-activated pannexin-1 |
Names |
Caspase-activated PANX1] |
Species |
Rattus norvegicus (Rat) |
KEGG Pathway |
rno:315435 |
EC number |
|
Protein Class |
|
Descriptions
Pannexin-1(Panx1) is the structural component of the gap junctions and the hemichannels involved in the ATP release and nucleotide permeation. Cleavage at Asp-379 removes a 46-amino acid C-terminal segment (380-426) that otherwise acts as an autoinhibitory blocker of the conducting pore formed by the Panx1 hexameric complex. Thus, accumulation of active caspase-3 during apoptosis can result in essentially irreversible open-gating of Panx1 channels.
Autoinhibitory domains (AIDs)
Target domain |
28-293 (Pannexin) |
Relief mechanism |
Cleavage |
Assay |
|
Accessory elements
No accessory elements
References
- Yeon JH et al. (2016) "Systems-wide Identification of cis-Regulatory Elements in Proteins", Cell systems, 2, 89-100
- Boyd-Tressler A et al. (2014) "Chemotherapeutic drugs induce ATP release via caspase-gated pannexin-1 channels and a caspase/pannexin-1-independent mechanism", The Journal of biological chemistry, 289, 27246-27263
- Mou L et al. (2020) "Structural basis for gating mechanism of Pannexin 1 channel", Cell research, 30, 452-454
Autoinhibited structure
Activated structure
1 structures for P60570
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-P60570-F1 | Predicted | AlphaFoldDB |
1 variants for P60570
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs3321206604 | 260 | D>N | No | EVA |
No associated diseases with P60570
No regional properties for P60570
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| No domain, repeats, and functional sites for P60570 | |||
6 GO annotations of cellular component
| Name | Definition |
|---|---|
| bleb | A cell extension caused by localized decoupling of the cytoskeleton from the plasma membrane and characterized by rapid formation, rounded shape, and scarcity of organelles within the protrusion. Blebs are formed during apoptosis and other cellular processes, including cell locomotion, cell division, and as a result of physical or chemical stresses. |
| endoplasmic reticulum | The irregular network of unit membranes, visible only by electron microscopy, that occurs in the cytoplasm of many eukaryotic cells. The membranes form a complex meshwork of tubular channels, which are often expanded into slitlike cavities called cisternae. The ER takes two forms, rough (or granular), with ribosomes adhering to the outer surface, and smooth (with no ribosomes attached). |
| endoplasmic reticulum membrane | The lipid bilayer surrounding the endoplasmic reticulum. |
| gap junction | A cell-cell junction composed of pannexins or innexins and connexins, two different families of channel-forming proteins. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
| protein-containing complex | A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together. |
14 GO annotations of molecular function
| Name | Definition |
|---|---|
| actin binding | Binding to monomeric or multimeric forms of actin, including actin filaments. |
| actin filament binding | Binding to an actin filament, also known as F-actin, a helical filamentous polymer of globular G-actin subunits. |
| ATP transmembrane transporter activity | Enables the transfer of ATP, adenosine triphosphate, from one side of a membrane to the other. |
| calcium channel activity | Enables the facilitated diffusion of a calcium ion (by an energy-independent process) involving passage through a transmembrane aqueous pore or channel without evidence for a carrier-mediated mechanism. |
| gap junction channel activity | A wide pore channel activity that enables a direct cytoplasmic connection from one cell to an adjacent cell. The gap junction can pass large solutes as well as electrical signals between cells. Gap junctions consist of two gap junction hemi-channels, or connexons, one contributed by each membrane through which the gap junction passes. |
| leak channel activity | Enables the transport of a solute across a membrane via a narrow pore channel that is open even in an unstimulated or 'resting' state. |
| monoatomic anion channel activity | Enables the energy-independent passage of a monoatomic anion across a lipid bilayer down a concentration gradient. |
| protease binding | Binding to a protease or a peptidase. |
| protein-containing complex binding | Binding to a macromolecular complex. |
| scaffold protein binding | Binding to a scaffold protein. Scaffold proteins are crucial regulators of many key signaling pathways. Although not strictly defined in function, they are known to interact and/or bind with multiple members of a signaling pathway, tethering them into complexes. |
| signaling receptor binding | Binding to one or more specific sites on a receptor molecule, a macromolecule that undergoes combination with a hormone, neurotransmitter, drug or intracellular messenger to initiate a change in cell function. |
| structural molecule activity | The action of a molecule that contributes to the structural integrity of a complex. |
| transmembrane transporter binding | Binding to a transmembrane transporter, a protein or protein complex that enables the transfer of a substance, usually a specific substance or a group of related substances, from one side of a membrane to the other. |
| wide pore channel activity | Enables the transport of a solute across a membrane via a large pore, un-gated channel. Examples include gap junctions, which transport substances from one cell to another; and porins which transport substances in and out of bacteria, mitochondria and chloroplasts. |
10 GO annotations of biological process
| Name | Definition |
|---|---|
| ATP transport | The directed movement of ATP, adenosine triphosphate, into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. |
| calcium ion transport | The directed movement of calcium (Ca) ions into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. |
| cell-cell signaling | Any process that mediates the transfer of information from one cell to another. This process includes signal transduction in the receiving cell and, where applicable, release of a ligand and any processes that actively facilitate its transport and presentation to the receiving cell. Examples include signaling via soluble ligands, via cell adhesion molecules and via gap junctions. |
| monoatomic anion transmembrane transport | The process in which a monoatomic anion is transported across a membrane. Monatomic anions (also called simple anions) are negatively charged ions consisting of exactly one atom. |
| monoatomic cation transport | The directed movement of a monoatomic cation, into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. Monatomic cations (also called simple cations) are positively charged ions consisting of exactly one atom. |
| positive regulation of interleukin-1 alpha production | Any process that activates or increases the frequency, rate, or extent of interleukin-1 alpha production. |
| positive regulation of interleukin-1 beta production | Any process that activates or increases the frequency, rate, or extent of interleukin-1 beta production. |
| positive regulation of macrophage cytokine production | Any process that increases the rate, frequency or extent of macrophage cytokine production. Macrophage cytokine production is the appearance of a chemokine due to biosynthesis or secretion following a cellular stimulus, resulting in an increase in its intracellular or extracellular levels. |
| response to ATP | Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an ATP (adenosine 5'-triphosphate) stimulus. |
| response to ischemia | Any process that results in a change in state or activity of an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a inadequate blood supply. |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MAIAHLATEY | VFSDFLLKEP | TEPKFKGLRL | ELAVDKMVTC | IAVGLPLLLI | SLAFAQEISI |
| 70 | 80 | 90 | 100 | 110 | 120 |
| GTQISCFSPS | SFSWRQAAFV | DSYCWAAVQQ | KNSLQSESGN | LPLWLHKFFP | YILLLFAILL |
| 130 | 140 | 150 | 160 | 170 | 180 |
| YLPALFWRFA | AAPHLCSDLK | FIMEELDKVY | NRAIKAAKSA | RDLDLRDGPG | PPGVTENVGQ |
| 190 | 200 | 210 | 220 | 230 | 240 |
| SLWEISESHF | KYPIVEQYLK | TKKNSSHLIM | KYISCRLVTF | AVVLLACIYL | SYYFSLSSLS |
| 250 | 260 | 270 | 280 | 290 | 300 |
| DEFLCSIKSG | VLRNDSTIPD | SFQCKLIAVG | IFQLLSLINL | LVYALLVPVV | IYTLFVPFRQ |
| 310 | 320 | 330 | 340 | 350 | 360 |
| KTDVLKVYEI | LPTFDVLHFK | SEGYNDLSLY | NLFLEENISE | LKSYKCLKVL | ENIKSNGQGI |
| 370 | 380 | 390 | 400 | 410 | 420 |
| DPMLLLTNLG | MIKMDVIDGK | VPMSLQTKGE | DQGSQRMDFK | DLDLSSETAA | NNGEKNSRQR |
| LLNSSC |