P59895

SS
Gene name
Nek6
Protein name
Serine/threonine-protein kinase Nek6
Names
Never in mitosis A-related kinase 6, NimA-related protein kinase 6
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:360161
EC number
Protein Class

Descriptions

Nek6 is a NIMA-related kinase essential for proper mitotic spindle assembly and has a catalytic domain with a 30-40 amino acid N-terminal extension. A human homolog Nek6 (Q9HC98) is subject to autoinhibition by the tyrosine motif, Tyr108. This is because the Y108A mutation is more active than wild-type Nek6. The same tyrosine motif is found in rat Nek6.

Autoinhibitory domains (AIDs)

108-114 (Tyrosine motif) SS

Target domain

31-41 (N-terminal extension of kinase domain)

Relief mechanism

Partner binding

Assay

Accessory elements

189-212 (Activation loop from InterPro)

Target domain

45-310 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for P59895

Entry ID Method Resolution Chain Position Source
AF-P59895-F1 Predicted AlphaFoldDB

No variants for P59895

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P59895

No associated diseases with P59895

7 regional properties for P59895

Type Name Position InterPro Accession
domain Protein kinase domain 45 - 310 IPR000719
domain Serine-threonine/tyrosine-protein kinase, catalytic domain 122 - 135 IPR001245-1
domain Serine-threonine/tyrosine-protein kinase, catalytic domain 162 - 180 IPR001245-2
domain Serine-threonine/tyrosine-protein kinase, catalytic domain 228 - 250 IPR001245-3
domain Serine-threonine/tyrosine-protein kinase, catalytic domain 274 - 296 IPR001245-4
active_site Serine/threonine-protein kinase, active site 168 - 180 IPR008271
binding_site Protein kinase, ATP binding site 51 - 74 IPR017441

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm
  • Nucleus
  • Nucleus speckle
  • Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
  • Cytoplasm, cytoskeleton, spindle pole
  • Colocalizes with APBB1 at the nuclear speckles
  • Colocalizes with PIN1 in the nucleus
  • Colocalizes with ATF4, CIR1, ARHGAP33, ANKRA2, CDC42, NEK9, RAD26L, RBBP6, RPS7, TRIP4, RELB and PHF1 in the centrosome
  • Localizes to spindle microtubules in metaphase and anaphase and to the midbody during cytokinesis (By similarity)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

7 GO annotations of cellular component

Name Definition
centriolar satellite A small (70-100 nm) cytoplasmic granule that contains a number of centrosomal proteins; centriolar satellites traffic toward microtubule minus ends and are enriched near the centrosome.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
nuclear speck A discrete extra-nucleolar subnuclear domain, 20-50 in number, in which splicing factors are seen to be localized by immunofluorescence microscopy.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
protein-containing complex A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together.
spindle pole Either of the ends of a spindle, where spindle microtubules are organized; usually contains a microtubule organizing center and accessory molecules, spindle microtubules and astral microtubules.

10 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
DNA-binding transcription factor binding Binding to a DNA-binding transcription factor, a protein that interacts with a specific DNA sequence (sometimes referred to as a motif) within the regulatory region of a gene to modulate transcription.
kinesin binding Interacting selectively and non-covalently and stoichiometrically with kinesin, a member of a superfamily of microtubule-based motor proteins that perform force-generating tasks such as organelle transport and chromosome segregation.
magnesium ion binding Binding to a magnesium (Mg) ion.
protein kinase binding Binding to a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
protein serine/threonine/tyrosine kinase activity Catalysis of the reactions: ATP + a protein serine = ADP + protein serine phosphate; ATP + a protein threonine = ADP + protein threonine phosphate; and ATP + a protein tyrosine = ADP + protein tyrosine phosphate.
transcription corepressor binding Binding to a transcription corepressor, a protein involved in negative regulation of transcription via protein-protein interactions with transcription factors and other proteins that negatively regulate transcription. Transcription corepressors do not bind DNA directly, but rather mediate protein-protein interactions between repressing transcription factors and the basal transcription machinery.
ubiquitin protein ligase binding Binding to a ubiquitin protein ligase enzyme, any of the E3 proteins.

8 GO annotations of biological process

Name Definition
apoptotic process A programmed cell death process which begins when a cell receives an internal (e.g. DNA damage) or external signal (e.g. an extracellular death ligand), and proceeds through a series of biochemical events (signaling pathway phase) which trigger an execution phase. The execution phase is the last step of an apoptotic process, and is typically characterized by rounding-up of the cell, retraction of pseudopodes, reduction of cellular volume (pyknosis), chromatin condensation, nuclear fragmentation (karyorrhexis), plasma membrane blebbing and fragmentation of the cell into apoptotic bodies. When the execution phase is completed, the cell has died.
cell cycle The progression of biochemical and morphological phases and events that occur in a cell during successive cell replication or nuclear replication events. Canonically, the cell cycle comprises the replication and segregation of genetic material followed by the division of the cell, but in endocycles or syncytial cells nuclear replication or nuclear division may not be followed by cell division.
cell division The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells.
chromosome segregation The process in which genetic material, in the form of chromosomes, is organized into specific structures and then physically separated and apportioned to two or more sets. In eukaryotes, chromosome segregation begins with the condensation of chromosomes, includes chromosome separation, and ends when chromosomes have completed movement to the spindle poles.
peptidyl-serine phosphorylation The phosphorylation of peptidyl-serine to form peptidyl-O-phospho-L-serine.
protein autophosphorylation The phosphorylation by a protein of one or more of its own amino acid residues (cis-autophosphorylation), or residues on an identical protein (trans-autophosphorylation).
protein phosphorylation The process of introducing a phosphate group on to a protein.
regulation of mitotic metaphase/anaphase transition Any process that modulates the frequency, rate or extent of the cell cycle process in which a cell progresses from metaphase to anaphase during mitosis, triggered by the activation of the anaphase promoting complex by Cdc20/Sleepy homolog which results in the degradation of Securin.

9 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q8TDX7 NEK7 Serine/threonine-protein kinase Nek7 Homo sapiens (Human) EV
Q9HC98 NEK6 Serine/threonine-protein kinase Nek6 Homo sapiens (Human) EV
Q9ES74 Nek7 Serine/threonine-protein kinase Nek7 Mus musculus (Mouse) SS
Q9ES70 Nek6 Serine/threonine-protein kinase Nek6 Mus musculus (Mouse) SS
A2BD05 NEK6 Serine/threonine-protein kinase Nek6 Sus scrofa (Pig) SS
D3ZBE5 Nek7 Serine/threonine-protein kinase Nek7 Rattus norvegicus (Rat) SS
G5EFM9 nekl-3 Serine/threonine-protein kinase nekl-3 Caenorhabditis elegans PR
Q9LVL5 WNK4 Probable serine/threonine-protein kinase WNK4 Arabidopsis thaliana (Mouse-ear cress) PR
Q9STK6 WNK3 Probable serine/threonine-protein kinase WNK3 Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MAGQPSHMPH GGSPNHLCHV LGPAHPPDPQ RHPNTLSFRC SLADFQIEKK IGRGQFSEVY
70 80 90 100 110 120
KATCLLDRKT VALKKVQIFE MMDAKARQDC VKEIGLLKQL NHPNIIKYLD SFIEDNELNI
130 140 150 160 170 180
VLELADAGDL SQMIKYFKKQ KRLIPERTVW KYFVQLCSAV EHMHSRRVMH RDIKPANVFI
190 200 210 220 230 240
TATGIVKLGD LGLGRFFSSE TTAAHSLVGT PYYMSPERIH ENGYNFKSDI WSLGCLLYEM
250 260 270 280 290 300
AALQSPFYGD KMNLFSLCQK IEQCDYPPLP GEHYSEKLRE LVSMCIYPDP NHRPDIEYVH
310
QVAKQMHVWT SST