P56536

EV
Gene name
Kif5c (Nkhc2)
Protein name
Kinesin heavy chain isoform 5C
Names
Kinesin heavy chain neuron-specific 2, Kinesin-1
Species
Rattus norvegicus (Rat)
KEGG Pathway
EC number
Protein Class
CENTROMERE PROTEIN E (PTHR47968)

Descriptions

Kinesin-1 is a plus-end microtubule-based motor and defects in kinesin-based transport are associated with diseases including neurodegeneration. Kinesin is autoinhibited through head-tail interactions. Kinesin-1 maintains an autoinhibited state through the KHC-tail domain, which interacts with the KHC motor domain, and through the KLC subunit, which physically separates the two KHC motor domains. JIP1 binding to KHC and KLC and JP3 binding to KLC can relieve the autoinhibition of kinesin-1.

Autoinhibitory domains (AIDs)

930-955 (tail) EV

Target domain

6-335 (Kinesin motor domain)

Relief mechanism

Partner binding

Assay

Deletion assay, Mutagenesis experiment

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

9 structures for P56536

Entry ID Method Resolution Chain Position Source
2KIN X-ray 200 A A 2-239 PDB
3KIN X-ray 310 A A/C 2-239 PDB
5HLE X-ray 290 A A 10-320 PDB
5HNW EM 660 A K 10-320 PDB
5HNX EM 660 A K 10-320 PDB
5HNY EM 630 A K 10-320 PDB
5HNZ EM 580 A K 10-320 PDB
8TT7 NMR - A/B 325-376 PDB
AF-P56536-F1 Predicted AlphaFoldDB

No variants for P56536

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P56536

No associated diseases with P56536

2 regional properties for P56536

Type Name Position InterPro Accession
domain Kinesin motor domain 6 - 335 IPR001752
conserved_site Kinesin motor domain, conserved site 226 - 237 IPR019821

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm, cytoskeleton
  • Cell projection, dendrite
  • Abundant in distal regions of dendrites
PANTHER Family PTHR47968 CENTROMERE PROTEIN E
PANTHER Subfamily PTHR47968:SF57 KINESIN FAMILY MEMBER 5C
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

11 GO annotations of cellular component

Name Definition
axon cytoplasm Any cytoplasm that is part of a axon.
axonal growth cone The migrating motile tip of a growing nerve cell axon.
ciliary rootlet A cytoskeleton-like structure, originating from the basal body at the proximal end of a cilium, and extending proximally toward the cell nucleus. Rootlets are typically 80-100 nm in diameter and contain cross striae distributed at regular intervals of approximately 55-70 nm.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
dendrite A neuron projection that has a short, tapering, morphology. Dendrites receive and integrate signals from other neurons or from sensory stimuli, and conduct nerve impulses towards the axon or the cell body. In most neurons, the impulse is conveyed from dendrites to axon via the cell body, but in some types of unipolar neuron, the impulse does not travel via the cell body.
dendrite cytoplasm All of the contents of a dendrite, excluding the surrounding plasma membrane.
distal axon That part of an axon close to and including the growth cone or the axon terminus.
kinesin complex Any complex that includes a dimer of molecules from the kinesin superfamily, a group of related proteins that contain an extended region of predicted alpha-helical coiled coil in the main chain that likely produces dimerization. The native complexes of several kinesin family members have also been shown to contain additional peptides, often designated light chains as all of the noncatalytic subunits that are currently known are smaller than the chain that contains the motor unit. Kinesin complexes generally possess a force-generating enzymatic activity, or motor, which converts the free energy of the gamma phosphate bond of ATP into mechanical work.
microtubule Any of the long, generally straight, hollow tubes of internal diameter 12-15 nm and external diameter 24 nm found in a wide variety of eukaryotic cells; each consists (usually) of 13 protofilaments of polymeric tubulin, staggered in such a manner that the tubulin monomers are arranged in a helical pattern on the microtubular surface, and with the alpha/beta axes of the tubulin subunits parallel to the long axis of the tubule; exist in equilibrium with pool of tubulin monomers and can be rapidly assembled or disassembled in response to physiological stimuli; concerned with force generation, e.g. in the spindle.
neuron projection A prolongation or process extending from a nerve cell, e.g. an axon or dendrite.
neuronal cell body The portion of a neuron that includes the nucleus, but excludes cell projections such as axons and dendrites.

6 GO annotations of molecular function

Name Definition
apolipoprotein receptor binding Binding to an apolipoprotein receptor.
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient.
microtubule binding Binding to a microtubule, a filament composed of tubulin monomers.
microtubule motor activity A motor activity that generates movement along a microtubule, driven by ATP hydrolysis.
plus-end-directed microtubule motor activity A motor activity that generates movement along a microtubule toward the plus end, driven by ATP hydrolysis.

11 GO annotations of biological process

Name Definition
anterograde axonal protein transport The directed movement of proteins along microtubules from the cell body toward the cell periphery in nerve cell axons.
anterograde dendritic transport of messenger ribonucleoprotein complex The directed movement of a messenger ribonucleoprotein complex along microtubules in nerve cell dendrites towards the postsynapse.
anterograde dendritic transport of neurotransmitter receptor complex The directed movement of a neurotransmitter receptor complex along microtubules in nerve cell dendrites towards the postsynapse.
ATP metabolic process The chemical reactions and pathways involving ATP, adenosine triphosphate, a universally important coenzyme and enzyme regulator.
axon guidance The chemotaxis process that directs the migration of an axon growth cone to a specific target site in response to a combination of attractive and repulsive cues.
cytoskeleton-dependent intracellular transport The directed movement of substances along cytoskeletal fibers such as microfilaments or microtubules within a cell.
intracellular mRNA localization Any process in which mRNA is transported to, or maintained in, a specific location within the cell.
microtubule-based movement A microtubule-based process that results in the movement of organelles, other microtubules, or other cellular components. Examples include motor-driven movement along microtubules and movement driven by polymerization or depolymerization of microtubules.
motor neuron axon guidance The process in which the migration of an axon growth cone of a motor neuron is directed to a specific target site in response to a combination of attractive and repulsive cues.
mRNA transport The directed movement of mRNA, messenger ribonucleic acid, into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
synaptic vesicle transport The directed movement of synaptic vesicles.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MADPAECSIK VMCRFRPLNE AEILRGDKFI PKFKGEETVV IGQGKPYVFD RVLPPNTTQE
70 80 90 100 110 120
QVYNACAKQI VKDVLEGYNG TIFAYGQTSS GKTHTMEGKL HDPQLMGIIP RIAHDIFDHI
130 140 150 160 170 180
YSMDENLEFH IKVSYFEIYL DKIRDLLDVS KTNLAVHEDK NRVPYVKGCT ERFVSSPEEV
190 200 210 220 230 240
MDVIDEGKAN RHVAVTNMNE HSSRSHSIFL INIKQENVET EKKLSGKLYL VDLAGSEKVS
250 260 270 280 290 300
KTGAEGAVLD EAKNINKSLS ALGNVISALA EGTKTHVPYR DSKMTRILQD SLGGNCRTTI
310 320 330 340 350 360
VICCSPSVFN EAETKSTLMF GQRAKTIKNT VSVNLELTAE EWKKKYEKEK EKNKALKSVI
370 380 390 400 410 420
QHLEVELNRW RNGEAVPEDE QISAKDQKNL EPCDNTPIID NITPVVDGIS AEKEKYDEEI
430 440 450 460 470 480
TSLYRQLDDK DDEINQQSQL AEKLKQQMLD QDELLASTRR DYEKIQEELT RLQIENEAAK
490 500 510 520 530 540
DEVKEVLQAL EELAVNYDQK SQEVEDKTRA NEQLTDELAQ KTTTLTTTQR ELSQLQELSN
550 560 570 580 590 600
HQKKRATEIL NLLLKDLGEI GGIIGTNDVK TLADVNGVIE EEFTMARLYI SKMKSEVKSL
610 620 630 640 650 660
VNRSKQLESA QTDSNRKMNA SERELAACQL LISQHEAKIK SLTDYMQNME QKRRQLEESQ
670 680 690 700 710 720
DSLSEELAKL RAQEKMHEVS FQDKEKEHLT RLQDAEEVKK ALEQQMESHR EAHQKQLSRL
730 740 750 760 770 780
RDEIEEKQRI IDEIRDLNQK LQLEQERLSS DYNKLKIEDQ EREVKLEKLL LLNDKREQAR
790 800 810 820 830 840
EDLKGLEETV SRELQTLHNL RKLFVQDLTT RVKKSVELDS DDGGGSAAQK QKISFLENNL
850 860 870 880 890 900
EQLTKVHKQL VRDNADLRCE LPKLEKRLRA TAERVKALES ALKEAKENAM RDRKRYQQEV
910 920 930 940 950
DRIKEAVRAK NMARRAHSAQ IAKPIRPGHY PASSPTAVHA VRGGGGSSNS THYQK