P56477

SS
Gene name
Irf5
Protein name
Interferon regulatory factor 5
Names
IRF-5
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:27056
EC number
Protein Class

Descriptions

IRF5 is a transcription factor that plays a critical role in innate immunity by activating expression of type I interferon. A human homolog IRF3 (Q14653) contains a C-terminal region containing phosphorylation sites. Phosphorylation of the sites converts IRF3 into a constitutively active form. The C-terminal autoinhibitory region intramolecularly interact with N-terminal region overlapping with an atypical transactivation domain. Thus, the C-terminal region similar with that of IRF3 may be involved in autoinhibition.

Autoinhibitory domains (AIDs)

430-497 (C-terminal autoinhibitory region) SS

Target domain

246-430 (IFN association domain)

Relief mechanism

PTM

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P56477

Entry ID Method Resolution Chain Position Source
AF-P56477-F1 Predicted AlphaFoldDB

3 variants for P56477

Variant ID(s) Position Change Description Diseaes Association Provenance
rs585125464 126 A>G No Ensembl
rs259714691 261 R>Q No Ensembl
rs258156314 495 G>S No Ensembl

No associated diseases with P56477

3 regional properties for P56477

Type Name Position InterPro Accession
domain Interferon regulatory factor, DNA-binding domain 10 - 123 IPR001346
domain Interferon regulatory factor-3 246 - 430 IPR019471
conserved_site Interferon regulatory factor, conserved site 35 - 68 IPR019817

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm
  • Nucleus
  • Shuttles between the nucleus and the cytoplasm: upon activation by the TLR adapter MYD88 and subsequent phosphorylation, translocates to the nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

7 GO annotations of molecular function

Name Definition
DNA-binding transcription activator activity, RNA polymerase II-specific A DNA-binding transcription factor activity that activates or increases transcription of specific gene sets transcribed by RNA polymerase II.
DNA-binding transcription factor activity, RNA polymerase II-specific A DNA-binding transcription factor activity that modulates the transcription of specific gene sets transcribed by RNA polymerase II.
identical protein binding Binding to an identical protein or proteins.
protein kinase binding Binding to a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate.
RNA polymerase II cis-regulatory region sequence-specific DNA binding Binding to a specific upstream regulatory DNA sequence (transcription factor recognition sequence or binding site) located in cis relative to the transcription start site (i.e., on the same strand of DNA) of a gene transcribed by RNA polymerase II.
sequence-specific DNA binding Binding to DNA of a specific nucleotide composition, e.g. GC-rich DNA binding, or with a specific sequence motif or type of DNA e.g. promotor binding or rDNA binding.
sequence-specific double-stranded DNA binding Binding to double-stranded DNA of a specific nucleotide composition, e.g. GC-rich DNA binding, or with a specific sequence motif or type of DNA, e.g. promotor binding or rDNA binding.

11 GO annotations of biological process

Name Definition
cytokine-mediated signaling pathway The series of molecular signals initiated by the binding of a cytokine to a receptor on the surface of a cell, and ending with the regulation of a downstream cellular process, e.g. transcription.
defense response to virus Reactions triggered in response to the presence of a virus that act to protect the cell or organism.
immune system process Any process involved in the development or functioning of the immune system, an organismal system for calibrated responses to potential internal or invasive threats.
inflammatory response The immediate defensive reaction (by vertebrate tissue) to infection or injury caused by chemical or physical agents. The process is characterized by local vasodilation, extravasation of plasma into intercellular spaces and accumulation of white blood cells and macrophages.
innate immune response Innate immune responses are defense responses mediated by germline encoded components that directly recognize components of potential pathogens.
positive regulation of apoptotic process Any process that activates or increases the frequency, rate or extent of cell death by apoptotic process.
positive regulation of transcription by RNA polymerase II Any process that activates or increases the frequency, rate or extent of transcription from an RNA polymerase II promoter.
positive regulation of type I interferon production Any process that activates or increases the frequency, rate, or extent of type I interferon production. Type I interferons include the interferon-alpha, beta, delta, episilon, zeta, kappa, tau, and omega gene families.
regulation of transcription by RNA polymerase II Any process that modulates the frequency, rate or extent of transcription mediated by RNA polymerase II.
response to muramyl dipeptide Any process that results in a change in state or activity of an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a muramyl dipeptide stimulus. Muramyl dipeptide is derived from peptidoglycan.
response to peptidoglycan Any process that results in a change in state or activity of an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a peptidoglycan stimulus. Peptidoglycan is a bacterial cell wall macromolecule.

7 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q58DJ0 IRF5 Interferon regulatory factor 5 Bos taurus (Bovine) SS
Q13568 IRF5 Interferon regulatory factor 5 Homo sapiens (Human) EV
Q61179 Irf9 Interferon regulatory factor 9 Mus musculus (Mouse) PR
Q64287 Irf4 Interferon regulatory factor 4 Mus musculus (Mouse) EV
P70671 Irf3 Interferon regulatory factor 3 Mus musculus (Mouse) SS
P70434 Irf7 Interferon regulatory factor 7 Mus musculus (Mouse) SS
P23611 Irf8 Interferon regulatory factor 8 Mus musculus (Mouse) PR
10 20 30 40 50 60
MNHSAPGIPP PPRRVRLKPW LVAQVNSCQY PGLQWVNGEK KLFYIPWRHA TRHGPSQDGD
70 80 90 100 110 120
NTIFKAWAKE TGKYTEGVDE ADPAKWKANL RCALNKSRDF QLFYDGPRDM PPQPYKIYEV
130 140 150 160 170 180
CSNGPAPTES QPTDDYVLGE EEEEEEEELQ RMLPGLSITE PALPGPPNAP YSLPKEDTKW
190 200 210 220 230 240
PPALQPPVGL GPPVPDPNLL APPSGNPAGF RQLLPEVLEP GPLASSQPPT EPLLPDLLIS
250 260 270 280 290 300
PHMLPLTDLE IKFQYRGRAP RTLTISNPQG CRLFYSQLEA TQEQVELFGP VTLEQVRFPS
310 320 330 340 350 360
PEDIPSDKQR FYTNQLLDVL DRGLILQLQG QDLYAIRLCQ CKVFWSGPCA LAHGSCPNPI
370 380 390 400 410 420
QREVKTKLFS LEQFLNELIL FQKGQTNTPP PFEIFFCFGE EWPDVKPREK KLITVQVVPV
430 440 450 460 470 480
AARLLLEMFS GELSWSADSI RLQISNPDLK DHMVEQFKEL HHLWQSQQQL QPMVQAPPVA
490
GLDASQGPWP MHPVGMQ