AiPD: Autoinhibited Protein Database

P55957

Gene name	BID
Protein name	BH3-interacting domain death agonist
Names	p22 BID, BID
Species	Homo sapiens (Human)
KEGG Pathway	hsa:637
EC number
Protein Class	BH3-INTERACTING DOMAIN DEATH AGONIST (PTHR35447)

Descriptions

BID is a proapoptotic member of the BCL2 family that shares only the BH3 domain homology with other members of the family in its amino acid sequence. The full-length BID is inactive and present in the cytosolic fraction of living cells. Upon cleavage by Caspase 8, the C-terminal part of BID translocates to mitochondria and is sufficient to trigger cytochrome c release in isolated mitochondria. The N-terminal segment may serve as an internal inhibitor of proapoptotic activity. The proapoptotic activity of BID is expected to require dissociation of the N-terminal segment including H1(14-29) and H2(33-41).

Autoinhibitory domains (AIDs)

Target domain	86-102 (BH3 domain)
Relief mechanism	Cleavage
Assay	Deletion assay, Structural analysis

AID

14-41 (N-terminal autoinhibitory segment including two helices H1 and H2)

Target domain

86-102 (BH3 domain)

Relief mechanism

Cleavage (Cleavage by Caspase-8)

Assay

Deletion assay, Structural analysis

Accessory elements

No accessory elements

References

Chou JJ et al. (1999) "Solution structure of BID, an intracellular amplifier of apoptotic signaling", Cell, 96, 615-24

Autoinhibited structure

Activated structure

17 structures for P55957

Entry ID	Method	Resolution	Chain	Position	Source
1ZY3	NMR	-	B	82-101	PDB
2BID	NMR	-	A	1-195	PDB
2KBW	NMR	-	B	76-106	PDB
2M5B	NMR	-	B	80-101	PDB
2M5I	NMR	-	A	61-195	PDB
4BD2	X-ray	221 A	C	76-109	PDB
4QVE	X-ray	205 A	B	76-109	PDB
4ZEQ	X-ray	180 A	B	79-104	PDB
4ZIG	X-ray	220 A	B	79-98	PDB
4ZII	X-ray	219 A	C	76-109	PDB
5AJJ	X-ray	175 A	B	79-112	PDB
5C3F	X-ray	143 A	B	80-101	PDB
7M5A	X-ray	150 A	B	80-100	PDB
7M5B	X-ray	185 A	B/D	80-100	PDB
7P33	X-ray	279 A	F/G/H/I/J	76-109	PDB
7QTW	X-ray	141 A	B	76-109	PDB
AF-P55957-F1	Predicted				AlphaFoldDB

168 variants for P55957

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
rs953741731 CA321235925	3	C>R		No	ClinGen TOPMed
CA410246443 rs1313841544	3	C>W		No	ClinGen gnomAD
CA410246258 rs1350104974	5	V>F		No	ClinGen TOPMed
CA410246245 rs1467042967	7	N>D		No	ClinGen TOPMed
CA410246236 rs1196785035 COSM579552	8	G>D	lung [Cosmic]	No	ClinGen cosmic curated gnomAD
rs770121452 CA10090941	8	G>S		No	ClinGen ExAC gnomAD
rs8190315 VAR_018845 CA10090940	10	S>G		No	ClinGen UniProt 1000Genomes ESP ExAC TOPMed dbSNP gnomAD
CA10090938 rs535265881	13	D>N		No	ClinGen 1000Genomes ExAC
rs899436087 CA321229353	14	E>A		No	ClinGen TOPMed
rs1225380451 CA410246183	16	I>F		No	ClinGen gnomAD
rs747539715 CA10090937	17	T>A		No	ClinGen ExAC TOPMed gnomAD
CA321229349 rs113033070	19	L>P		No	ClinGen Ensembl
rs940864168 CA321229344	22	F>C		No	ClinGen TOPMed
CA10090935 rs758837211	22	F>L		No	ClinGen ExAC TOPMed gnomAD
rs1231804680 CA410246109	27	S>R		No	ClinGen gnomAD
CA410246096 rs1331193773	29	S>C		No	ClinGen gnomAD
CA10090931 rs753398808	31	N>K		No	ClinGen ExAC gnomAD
CA410246065 rs1391991356	33	F>L		No	ClinGen TOPMed
rs764590275 CA10090930	34	R>C		No	ClinGen ExAC TOPMed gnomAD
rs760132280 CA10090929	34	R>H		No	ClinGen ExAC TOPMed gnomAD
CA10090928 rs199617244	35	R>G		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs767379821 CA10090927	35	R>I		No	ClinGen ExAC
rs761738989 CA321229311	36	E>D		No	ClinGen ExAC TOPMed gnomAD
rs372024838 CA10090924	39	A>T		No	ClinGen ESP ExAC TOPMed gnomAD
CA10090923 rs759844569	39	A>V		No	ClinGen ExAC
rs1284531075 CA410246022	41	G>D		No	ClinGen gnomAD
CA10090922 rs776679558	41	G>S		No	ClinGen ExAC gnomAD
CA410246013 rs150608132	42	H>Q		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA410246016 rs1336284212	42	H>R		No	ClinGen Ensembl
CA410246011 rs747016844	43	E>*		No	ClinGen ExAC TOPMed gnomAD
CA410246010 rs1251759596	43	E>G		No	ClinGen gnomAD
rs747016844 CA10090920	43	E>K		No	ClinGen ExAC TOPMed gnomAD
rs777994982 CA10090919	46	V>A		No	ClinGen ExAC TOPMed gnomAD
CA410245984 rs1199515354	48	A>T		No	ClinGen gnomAD
CA410245976 rs1284609688	49	P>L		No	ClinGen gnomAD
CA10090917 rs748577760	49	P>S		No	ClinGen ExAC gnomAD
rs11542850 CA321229268	50	Q>*		No	ClinGen gnomAD
rs11542850 CA410245972	50	Q>E		No	ClinGen gnomAD
CA10090916 rs779372119	52	E>*		No	ClinGen ExAC gnomAD
CA10090914 rs753553004	53	G>D		No	ClinGen ExAC gnomAD
CA10090915 rs755255176	53	G>S		No	ClinGen ExAC gnomAD
rs1333326559 CA410245937	55	D>H		No	ClinGen gnomAD
rs1333326559 CA410245938	55	D>N		No	ClinGen gnomAD
CA410245930 rs1257785042	56	E>K		No	ClinGen Ensembl
rs879161693 CA321229259	57	L>P		No	ClinGen Ensembl
CA321229258 rs922447901	58	Q>*		No	ClinGen TOPMed
CA10090912 rs755629886	58	Q>R		No	ClinGen ExAC TOPMed gnomAD
rs1455741313 CA410245898	61	G>S		No	ClinGen gnomAD
rs1411787711 CA410245892	62	N>H		No	ClinGen gnomAD
CA10090909 rs756092459	62	N>I		No	ClinGen ExAC TOPMed gnomAD
CA10090908 rs756092459	62	N>T		No	ClinGen ExAC TOPMed gnomAD
rs762595889 CA10090906	63	R>C		No	ClinGen ExAC TOPMed gnomAD
CA10090905 rs771344517	63	R>H		No	ClinGen ExAC TOPMed gnomAD
rs771344517 CA10090904	63	R>P		No	ClinGen ExAC TOPMed gnomAD
rs762595889 CA10090907	63	R>S		No	ClinGen ExAC TOPMed gnomAD
rs773469812 CA10090902	64	S>R		No	ClinGen ExAC gnomAD
rs201438109 CA10090901	64	S>T		No	ClinGen ExAC gnomAD
rs748717824 CA410245871	65	S>R		No	ClinGen ExAC TOPMed gnomAD
rs61757628 CA10090898	68	R>C		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs769081918 CA10090897	68	R>H		No	ClinGen ExAC TOPMed gnomAD
rs749551829 CA10090896	69	L>F		No	ClinGen ExAC gnomAD
rs1365510991 CA410245264	75	D>G		No	ClinGen TOPMed gnomAD
CA410245262 rs1365510991	75	D>V		No	ClinGen TOPMed gnomAD
CA10090829 rs768772704	76	S>C		No	ClinGen ExAC
rs779754238 CA10090827	77	E>G		No	ClinGen ExAC gnomAD
CA10090828 rs553170594	77	E>K		No	ClinGen 1000Genomes ExAC gnomAD
rs755901638 CA10090826	80	E>V		No	ClinGen ExAC gnomAD
rs1462876451 CA410245154	81	D>N		No	ClinGen gnomAD
rs1569038087 CA410245132	82	I>V		No	ClinGen Ensembl
rs1246232157 CA410245116	83	I>V		No	ClinGen gnomAD
CA410245096 rs148107209	84	R>G		No	ClinGen ESP ExAC TOPMed gnomAD
rs777997323 CA10090824	84	R>Q		No	ClinGen ExAC TOPMed gnomAD
CA10090825 rs148107209	84	R>W		No	ClinGen ESP ExAC TOPMed gnomAD
CA321226005 rs991416275	85	N>K		No	ClinGen gnomAD
CA410245060 rs1399322019	85	N>S		No	ClinGen TOPMed
CA10090823 rs758479946	86	I>T		No	ClinGen ExAC gnomAD
rs758447724 CA10090822	88	R>K		No	ClinGen ExAC TOPMed gnomAD
CA10090821 rs765274901	89	H>R		No	ClinGen ExAC gnomAD
rs375433200 COSM1184706 CA10090819	91	A>T	large_intestine [Cosmic]	No	ClinGen cosmic curated ESP ExAC TOPMed gnomAD
CA10090817 rs761042336	94	G>R		No	ClinGen ExAC gnomAD
CA410244863 rs1291732520	96	S>N		No	ClinGen TOPMed gnomAD
CA410244837 rs1487631102	97	M>I		No	ClinGen TOPMed
COSM1580029 CA10090816 rs772742590	97	M>V	central_nervous_system [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
CA410244813 rs1193532159	98	D>E		No	ClinGen TOPMed
CA10090815 rs556890832	98	D>G		No	ClinGen 1000Genomes ExAC gnomAD
CA10090814 rs537058757	99	R>C		No	ClinGen 1000Genomes ExAC gnomAD
rs370897535 CA10090813	99	R>H		No	ClinGen ESP ExAC TOPMed gnomAD
CA410244807 rs537058757	99	R>S		No	ClinGen 1000Genomes ExAC gnomAD
CA10090808 rs775453548	102	P>L		No	ClinGen ExAC gnomAD
rs575351241 CA10090807	103	P>L		No	ClinGen ExAC TOPMed gnomAD
rs1292917414 CA410244712	104	G>S		No	ClinGen gnomAD
rs780833600 CA10090805	104	G>V		No	ClinGen ExAC gnomAD
rs143734092 CA10090804	105	L>P		No	ClinGen ESP ExAC TOPMed gnomAD
CA10090803 rs748334233	106	V>G		No	ClinGen ExAC TOPMed gnomAD
CA321225882 rs143198074	108	G>S		No	ClinGen ESP TOPMed gnomAD
CA321225859 rs867403167	110	A>T		No	ClinGen Ensembl
rs372763830 CA10090800	113	L>F		No	ClinGen ESP ExAC TOPMed gnomAD
rs766841317 CA10090799	115	N>T		No	ClinGen ExAC gnomAD
rs767973661 CA10090796	116	T>I		No	ClinGen ExAC gnomAD
rs200270687 CA10090797	116	T>P		No	ClinGen ExAC gnomAD
rs763724206 CA10090793	118	R>Q		No	ClinGen ExAC TOPMed gnomAD
rs774106967 CA10090794	118	R>W		No	ClinGen ExAC TOPMed gnomAD
CA10090792 COSM1246110 rs762470911	119	S>L	oesophagus [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
CA410244488 rs1569037832	119	S>T		No	ClinGen Ensembl
COSM1031879 rs146769173 CA10090790	120	E>D	endometrium [Cosmic]	No	ClinGen cosmic curated ESP ExAC TOPMed gnomAD
CA10090791 rs139202406	120	E>G		No	ClinGen ESP ExAC TOPMed gnomAD
rs1039824902 CA321225807	120	E>K		No	ClinGen Ensembl
CA10090789 rs745767890	121	E>V		No	ClinGen ExAC gnomAD
CA10090764 rs142609840	123	R>Q		No	ClinGen ESP ExAC TOPMed gnomAD
rs749451392 CA10090765	123	R>W		No	ClinGen ExAC TOPMed gnomAD
rs746410035 CA10090762	125	R>K		No	ClinGen ExAC TOPMed gnomAD
CA410243771 rs1159470596	126	D>Y		No	ClinGen gnomAD
rs1471365359 CA410243756	127	L>V		No	ClinGen gnomAD
rs925525205 CA321224953	133	Q>H		No	ClinGen Ensembl
rs959646977 CA321224956	133	Q>R		No	ClinGen gnomAD
rs895511972 CA410243614	135	L>P		No	ClinGen TOPMed
CA321224948 rs895511972	135	L>R		No	ClinGen TOPMed
rs145415034 CA321224933	136	Q>*		No	ClinGen ESP TOPMed
rs539560010 CA10090761	136	Q>H		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs1232265246 CA410243567	138	Y>*		No	ClinGen Ensembl
rs1225189516 CA410243557	140	R>G		No	ClinGen TOPMed gnomAD
rs1330293831 CA410243515	142	M>I		No	ClinGen TOPMed
rs752039343 CA10090759	142	M>L		No	ClinGen ExAC TOPMed gnomAD
CA410243499 rs1314201369	143	E>G		No	ClinGen gnomAD
rs1245484966 CA410243487	144	K>E		No	ClinGen gnomAD
CA321224914 rs967098884	145	E>K		No	ClinGen TOPMed
CA10090754 rs140738257	148	M>I		No	ClinGen ESP ExAC TOPMed gnomAD
rs150084106 CA10090755	148	M>T		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs778272070 CA10090756	148	M>V		No	ClinGen ExAC gnomAD
rs1601833850 CA410243352	151	L>V		No	ClinGen Ensembl
rs753778329 CA10090751	156	A>T		No	ClinGen ExAC gnomAD
CA10090750 rs766369895	157	K>E		No	ClinGen ExAC
rs773039137 CA10090747	158	K>N		No	ClinGen ExAC gnomAD
CA10090748 rs760590436	158	K>R		No	ClinGen ExAC gnomAD
CA10090746 rs771733957	160	A>S		No	ClinGen ExAC TOPMed gnomAD
CA10090745 rs763268339	160	A>V		No	ClinGen ExAC gnomAD
rs17853595 CA321224772 VAR_025332	162	H>Q		No	ClinGen UniProt Ensembl dbSNP
rs547322681 CA10090743	163	T>M		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs745881133 CA10090741	164	P>L		No	ClinGen ExAC TOPMed gnomAD
rs745881133 CA10090742	164	P>R		No	ClinGen ExAC TOPMed gnomAD
rs1290735330 CA410243142	164	P>S		No	ClinGen gnomAD
rs747484734 CA10090738	166	L>V		No	ClinGen ExAC gnomAD
CA10090737 rs778228540	167	L>P		No	ClinGen ExAC TOPMed gnomAD
CA10090736 rs185077891	168	R>C		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA410243074 COSM444680 rs1270911833	168	R>H	breast [Cosmic]	No	ClinGen cosmic curated TOPMed gnomAD
rs752404269 CA10090735	169	D>N		No	ClinGen ExAC gnomAD
CA410243054 rs778362797	170	V>I		No	ClinGen ExAC TOPMed gnomAD
CA10090734 rs778362797	170	V>L		No	ClinGen ExAC TOPMed gnomAD
rs376912423 CA10090733	171	F>V		No	ClinGen ESP ExAC
rs1425719027 CA410242988	173	T>I		No	ClinGen TOPMed gnomAD
rs1425719027 CA410242990	173	T>K		No	ClinGen TOPMed gnomAD
CA10090728 rs1427627939	180	Q>R		No	ClinGen gnomAD
CA10090727 rs750323234	181	N>H		No	ClinGen ExAC gnomAD
CA10090726 rs367829996	181	N>K		No	ClinGen ESP ExAC TOPMed gnomAD
rs538615870 CA10090725	183	R>C		No	ClinGen ExAC TOPMed gnomAD
rs538615870 CA410242874	183	R>G		No	ClinGen ExAC TOPMed gnomAD
rs139212717 CA10090724	183	R>H		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs139212717 CA321224632	183	R>P		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs1228391650 CA410242865	184	T>N		No	ClinGen TOPMed
rs771091033 CA10090720	186	V>G		No	ClinGen ExAC gnomAD
rs61752236 CA10090721	186	V>L		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA10090722 rs61752236	186	V>M		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs1273774956 CA410242806	190	A>T		No	ClinGen gnomAD
rs550005605 CA10090718	192	N>T		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs774617687 CA410242305	193	G>R		No	ClinGen ExAC TOPMed gnomAD
rs774617687 CA10090696	193	G>R		No	ClinGen ExAC TOPMed gnomAD
CA410242279 rs1569035683	194	M>I		No	ClinGen Ensembl
rs59225839 CA10090694 VAR_061041	194	M>T		No	ClinGen UniProt 1000Genomes ESP ExAC TOPMed dbSNP gnomAD

No associated diseases with P55957

6 regional properties for P55957

Type	Name	Position	InterPro Accession
domain	Coagulation factor 5/8 C-terminal domain	30 - 185	IPR000421
domain	Protein kinase domain	610 - 905	IPR000719
domain	Serine-threonine/tyrosine-protein kinase, catalytic domain	610 - 905	IPR001245
conserved_site	Tyrosine-protein kinase, receptor class II, conserved site	790 - 798	IPR002011
active_site	Tyrosine-protein kinase, active site	762 - 774	IPR008266
domain	Tyrosine-protein kinase, catalytic domain	610 - 905	IPR020635

Functions

		Description
EC Number
Subcellular Localization	Cytoplasm Mitochondrion membrane Mitochondrion outer membrane	When uncleaved, it is predominantly cytoplasmic
PANTHER Family	PTHR35447	BH3-INTERACTING DOMAIN DEATH AGONIST
PANTHER Subfamily	PTHR35447:SF1	BH3-INTERACTING DOMAIN DEATH AGONIST
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

5 GO annotations of cellular component

Name	Definition
cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
integral component of mitochondrial membrane	The component of the mitochondrial membrane consisting of the gene products having at least some part of their peptide sequence embedded in the hydrophobic region of the membrane.
membrane	A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.
mitochondrial outer membrane	The outer, i.e. cytoplasm-facing, lipid bilayer of the mitochondrial envelope.
mitochondrion	A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.

2 GO annotations of molecular function

Name	Definition
death receptor binding	Binding to a member of the death receptor (DR) family. The DR family falls within the tumor necrosis factor receptor superfamily and is characterized by a cytoplasmic region of ~80 residues termed the death domain (DD).
ubiquitin protein ligase binding	Binding to a ubiquitin protein ligase enzyme, any of the E3 proteins.

23 GO annotations of biological process

Name	Definition
activation of cysteine-type endopeptidase activity involved in apoptotic process	Any process that initiates the activity of the inactive enzyme cysteine-type endopeptidase in the context of an apoptotic process.
apoptotic mitochondrial changes	The morphological and physiological alterations undergone by mitochondria during apoptosis.
establishment of protein localization to membrane	The directed movement of a protein to a specific location in a membrane.
extrinsic apoptotic signaling pathway via death domain receptors	The series of molecular signals in which a signal is conveyed from the cell surface to trigger the apoptotic death of a cell. The pathway starts with a ligand binding to a death domain receptor on the cell surface, and ends when the execution phase of apoptosis is triggered.
hepatocyte apoptotic process	Any apoptotic process in a hepatocyte, the main structural component of the liver.
mitochondrial ATP synthesis coupled electron transport	The transfer of electrons through a series of electron donors and acceptors, generating energy that is ultimately used for synthesis of ATP, as it occurs in the mitochondrial inner membrane or chloroplast thylakoid membrane.
mitochondrial outer membrane permeabilization	The process by which the mitochondrial outer membrane becomes permeable to the passing of proteins and other molecules from the intermembrane space to the cytosol as part of the apoptotic signaling pathway.
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage	Any process that stops, prevents or reduces the frequency, rate or extent of intrinsic apoptotic signaling pathway in response to DNA damage.
neuron apoptotic process	Any apoptotic process in a neuron, the basic cellular unit of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the nervous system.
positive regulation of apoptotic process	Any process that activates or increases the frequency, rate or extent of cell death by apoptotic process.
positive regulation of extrinsic apoptotic signaling pathway	Any process that activates or increases the frequency, rate or extent of extrinsic apoptotic signaling pathway.
positive regulation of fibroblast apoptotic process	Any process that activates or increases the frequency, rate or extent of fibroblast apoptotic process.
positive regulation of intrinsic apoptotic signaling pathway	Any process that activates or increases the frequency, rate or extent of intrinsic apoptotic signaling pathway.
positive regulation of mitochondrial membrane potential	Any process that activates or increases the frequency, rate or extent of establishment or extent of a mitochondrial membrane potential, the electric potential existing across any mitochondrial membrane arising from charges in the membrane itself and from the charges present in the media on either side of the membrane.
positive regulation of protein-containing complex assembly	Any process that activates or increases the frequency, rate or extent of protein complex assembly.
positive regulation of release of cytochrome c from mitochondria	Any process that increases the rate, frequency or extent of release of cytochrome c from mitochondria, the process in which cytochrome c is enabled to move from the mitochondrial intermembrane space into the cytosol, which is an early step in apoptosis and leads to caspase activation.
protein targeting to mitochondrion	The process of directing proteins towards and into the mitochondrion, usually mediated by mitochondrial proteins that recognize signals contained within the imported protein.
protein-containing complex assembly	The aggregation, arrangement and bonding together of a set of macromolecules to form a protein-containing complex.
regulation of cell population proliferation	Any process that modulates the frequency, rate or extent of cell proliferation.
regulation of G1/S transition of mitotic cell cycle	Any signalling pathway that modulates the activity of a cell cycle cyclin-dependent protein kinase to modulate the switch from G1 phase to S phase of the mitotic cell cycle.
release of cytochrome c from mitochondria	The process that results in the movement of cytochrome c from the mitochondrial intermembrane space into the cytosol, which is part of the apoptotic signaling pathway and leads to caspase activation.
signal transduction in response to DNA damage	A cascade of processes induced by the detection of DNA damage within a cell.
supramolecular fiber organization	A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of a supramolecular fiber, a polymer consisting of an indefinite number of protein or protein complex subunits that have polymerised to form a fiber-shaped structure.

3 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
P70444	Bid	BH3-interacting domain death agonist	Mus musculus (Mouse)	PR
Q4JHS0	BID	BH3-interacting domain death agonist	Sus scrofa (Pig)	SS
Q9JLT6	Bid	BH3-interacting domain death agonist	Rattus norvegicus (Rat)	PR

10	20	30	40	50	60
MDCEVNNGSS	LRDECITNLL	VFGFLQSCSD	NSFRRELDAL	GHELPVLAPQ	WEGYDELQTD
70	80	90	100	110	120
GNRSSHSRLG	RIEADSESQE	DIIRNIARHL	AQVGDSMDRS	IPPGLVNGLA	LQLRNTSRSE
130	140	150	160	170	180
EDRNRDLATA	LEQLLQAYPR	DMEKEKTMLV	LALLLAKKVA	SHTPSLLRDV	FHTTVNFINQ
190
NLRTYVRSLA	RNGMD