P54757
SS
Gene name |
Epha5 (Ehk-1, Ekh1, Rek7) |
Protein name |
Ephrin type-A receptor 5 |
Names |
EC 2.7.10.1 , EPH homology kinase 1 , EHK-1 |
Species |
Rattus norvegicus (Rat) |
KEGG Pathway |
rno:79208 |
EC number |
2.7.10.1: Protein-tyrosine kinases |
Protein Class |
|
Descriptions
Ephrin type-B receptor 2 is a membrane-associated protein that mediates axon guidance, cell migration and morphogenesis. The Eph receptor tyrosine kinase family is regulated by autophosphorylation within the juxtamembrane region and the kinase activation segment. The structure, supported by mutagenesis data, reveals that the juxtamembrane segment adopts a helical conformation that distorts the small lobe of the kinase domain, and blocks the activation segment from attaining an activated conformation. Phosphorylation of the conserved juxtamembrane tyrosines would relieve this autoinhibition by disturbing the association of the juxtamembrane segment with the kinase domain, while liberating phosphotyrosine sites for binding SH2 domains of target proteins.
Autoinhibitory domains (AIDs)
Target domain |
677-938 (Protein kinase domain) |
Relief mechanism |
PTM |
Assay |
|
Accessory elements
819-845 (Activation loop from InterPro)
Target domain |
677-938 (Protein kinase domain) |
Relief mechanism |
|
Assay |
|
References
Autoinhibited structure
Activated structure
1 structures for P54757
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-P54757-F1 | Predicted | AlphaFoldDB |
No variants for P54757
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for P54757 | |||||
No associated diseases with P54757
12 regional properties for P54757
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Protein kinase domain | 677 - 938 | IPR000719 |
| domain | Ephrin receptor ligand binding domain | 62 - 240 | IPR001090 |
| domain | Serine-threonine/tyrosine-protein kinase, catalytic domain | 677 - 934 | IPR001245 |
| conserved_site | Tyrosine-protein kinase, receptor class V, conserved site | 216 - 236 | IPR001426-1 |
| conserved_site | Tyrosine-protein kinase, receptor class V, conserved site | 278 - 298 | IPR001426-2 |
| domain | Sterile alpha motif domain | 967 - 1005 | IPR001660 |
| domain | Fibronectin type III | 359 - 469 | IPR003961-1 |
| domain | Fibronectin type III | 470 - 564 | IPR003961-2 |
| active_site | Tyrosine-protein kinase, active site | 798 - 810 | IPR008266 |
| domain | Tyrosine-protein kinase ephrin type A/B receptor-like | 299 - 337 | IPR011641 |
| domain | Tyrosine-protein kinase, catalytic domain | 677 - 934 | IPR020635 |
| domain | Ephrin receptor, transmembrane domain | 578 - 674 | IPR027936 |
Functions
| Description | ||
|---|---|---|
| EC Number | 2.7.10.1 | Protein-tyrosine kinases |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
9 GO annotations of cellular component
| Name | Definition |
|---|---|
| adherens junction | A cell-cell junction composed of the epithelial cadherin-catenin complex. The epithelial cadherins, or E-cadherins, of each interacting cell extend through the plasma membrane into the extracellular space and bind to each other. The E-cadherins bind to catenins on the cytoplasmic side of the membrane, where the E-cadherin-catenin complex binds to cytoskeletal components and regulatory and signaling molecules. |
| axon | The long process of a neuron that conducts nerve impulses, usually away from the cell body to the terminals and varicosities, which are sites of storage and release of neurotransmitter. |
| cell periphery | The broad region around and including the plasma membrane of a cell, encompassing the cell cortex (inside the cell), the plasma membrane, and any external encapsulating structures. |
| dendrite | A neuron projection that has a short, tapering, morphology. Dendrites receive and integrate signals from other neurons or from sensory stimuli, and conduct nerve impulses towards the axon or the cell body. In most neurons, the impulse is conveyed from dendrites to axon via the cell body, but in some types of unipolar neuron, the impulse does not travel via the cell body. |
| external side of plasma membrane | The leaflet of the plasma membrane that faces away from the cytoplasm and any proteins embedded or anchored in it or attached to its surface. |
| neuronal cell body | The portion of a neuron that includes the nucleus, but excludes cell projections such as axons and dendrites. |
| perinuclear region of cytoplasm | Cytoplasm situated near, or occurring around, the nucleus. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
| rough endoplasmic reticulum | The rough (or granular) endoplasmic reticulum (ER) has ribosomes adhering to the outer surface; the ribosomes are the site of translation of the mRNA for those proteins which are either to be retained within the cisternae (ER-resident proteins), the proteins of the lysosomes, or the proteins destined for export from the cell. Glycoproteins undergo their initial glycosylation within the cisternae. |
5 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| ephrin receptor activity | Combining with an ephrin receptor ligand to initiate a change in cell activity. |
| GPI-linked ephrin receptor activity | Combining with a GPI-anchored ephrin to initiate a change in cell activity. |
| growth factor binding | Binding to a growth factor, proteins or polypeptides that stimulate a cell or organism to grow or proliferate. |
| transmembrane-ephrin receptor activity | Combining with a transmembrane ephrin to initiate a change in cell activity. |
14 GO annotations of biological process
| Name | Definition |
|---|---|
| axon guidance | The chemotaxis process that directs the migration of an axon growth cone to a specific target site in response to a combination of attractive and repulsive cues. |
| cAMP-mediated signaling | Any intracellular signal transduction in which the signal is passed on within the cell via cyclic AMP (cAMP). Includes production of cAMP, and downstream effectors that further transmit the signal within the cell. |
| cellular response to follicle-stimulating hormone stimulus | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a follicle-stimulating hormone stimulus. |
| cellular response to forskolin | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a forskolin stimulus. |
| dendritic spine morphogenesis | The process in which the anatomical structures of a dendritic spine are generated and organized. A dendritic spine is a protrusion from a dendrite and a specialized subcellular compartment involved in synaptic transmission. |
| ephrin receptor signaling pathway | The series of molecular signals initiated by ephrin binding to its receptor, and ending with the regulation of a downstream cellular process, e.g. transcription. |
| hippocampus development | The progression of the hippocampus over time from its initial formation until its mature state. |
| negative regulation of cell adhesion | Any process that stops, prevents, or reduces the frequency, rate or extent of cell adhesion. |
| neuron development | The process whose specific outcome is the progression of a neuron over time, from initial commitment of the cell to a specific fate, to the fully functional differentiated cell. |
| phosphorylation | The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide. |
| positive regulation of CREB transcription factor activity | Any process that activates or increases the frequency, rate or extent of activity of the transcription factor CREB. |
| regulation of actin cytoskeleton organization | Any process that modulates the frequency, rate or extent of the formation, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising actin filaments and their associated proteins. |
| regulation of GTPase activity | Any process that modulates the rate of GTP hydrolysis by a GTPase. |
| regulation of insulin secretion involved in cellular response to glucose stimulus | Any process that modulates the frequency, rate or extent of the regulated release of insulin that contributes to the response of a cell to glucose. |
52 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P28693 | EPHB2 | Ephrin type-B receptor 2 | Gallus gallus (Chicken) | PR |
| Q07496 | EPHA4 | Ephrin type-A receptor 4 | Gallus gallus (Chicken) | SS |
| Q07494 | EPHB1 | Ephrin type-B receptor 1 | Gallus gallus (Chicken) | SS |
| Q07497 | EPHB5 | Ephrin type-B receptor 5 | Gallus gallus (Chicken) | PR |
| P29318 | EPHA3 | Ephrin type-A receptor 3 | Gallus gallus (Chicken) | SS |
| O42422 | EPHA7 | Ephrin type-A receptor 7 | Gallus gallus (Chicken) | SS |
| Q07498 | EPHB3 | Ephrin type-B receptor 3 | Gallus gallus (Chicken) | SS |
| P54755 | EPHA5 | Ephrin type-A receptor 5 | Gallus gallus (Chicken) | SS |
| P0C0K6 | EPHB6 | Ephrin type-B receptor 6 | Pan troglodytes (Chimpanzee) | SS |
| P29322 | EPHA8 | Ephrin type-A receptor 8 | Homo sapiens (Human) | SS |
| P21709 | EPHA1 | Ephrin type-A receptor 1 | Homo sapiens (Human) | SS |
| P54764 | EPHA4 | Ephrin type-A receptor 4 | Homo sapiens (Human) | SS |
| P54753 | EPHB3 | Ephrin type-B receptor 3 | Homo sapiens (Human) | SS |
| P29320 | EPHA3 | Ephrin type-A receptor 3 | Homo sapiens (Human) | PR |
| P54760 | EPHB4 | Ephrin type-B receptor 4 | Homo sapiens (Human) | SS |
| P29317 | EPHA2 | Ephrin type-A receptor 2 | Homo sapiens (Human) | SS |
| Q15375 | EPHA7 | Ephrin type-A receptor 7 | Homo sapiens (Human) | SS |
| Q5JZY3 | EPHA10 | Ephrin type-A receptor 10 | Homo sapiens (Human) | SS |
| P29323 | EPHB2 | Ephrin type-B receptor 2 | Homo sapiens (Human) | EV |
| Q9UF33 | EPHA6 | Ephrin type-A receptor 6 | Homo sapiens (Human) | SS |
| P54762 | EPHB1 | Ephrin type-B receptor 1 | Homo sapiens (Human) | SS |
| O15197 | EPHB6 | Ephrin type-B receptor 6 | Homo sapiens (Human) | SS |
| P54756 | EPHA5 | Ephrin type-A receptor 5 | Homo sapiens (Human) | SS |
| P54754 | Ephb3 | Ephrin type-B receptor 3 | Mus musculus (Mouse) | SS |
| Q03145 | Epha2 | Ephrin type-A receptor 2 | Mus musculus (Mouse) | PR |
| Q03137 | Epha4 | Ephrin type-A receptor 4 | Mus musculus (Mouse) | SS |
| Q61772 | Epha7 | Ephrin type-A receptor 7 | Mus musculus (Mouse) | SS |
| O09127 | Epha8 | Ephrin type-A receptor 8 | Mus musculus (Mouse) | SS |
| Q8CBF3 | Ephb1 | Ephrin type-B receptor 1 | Mus musculus (Mouse) | SS |
| Q8BYG9 | Epha10 | Ephrin type-A receptor 10 | Mus musculus (Mouse) | SS |
| P29319 | Epha3 | Ephrin type-A receptor 3 | Mus musculus (Mouse) | SS |
| Q62413 | Epha6 | Ephrin type-A receptor 6 | Mus musculus (Mouse) | SS |
| Q60750 | Epha1 | Ephrin type-A receptor 1 | Mus musculus (Mouse) | SS |
| P54763 | Ephb2 | Ephrin type-B receptor 2 | Mus musculus (Mouse) | SS |
| P54761 | Ephb4 | Ephrin type-B receptor 4 | Mus musculus (Mouse) | PR |
| O08644 | Ephb6 | Ephrin type-B receptor 6 | Mus musculus (Mouse) | PR |
| Q60629 | Epha5 | Ephrin type-A receptor 5 | Mus musculus (Mouse) | SS |
| P54759 | Epha7 | Ephrin type-A receptor 7 | Rattus norvegicus (Rat) | SS |
| P09759 | Ephb1 | Ephrin type-B receptor 1 | Rattus norvegicus (Rat) | SS |
| O08680 | Epha3 | Ephrin type-A receptor 3 | Rattus norvegicus (Rat) | SS |
| P0C0K7 | Ephb6 | Ephrin type-B receptor 6 | Rattus norvegicus (Rat) | SS |
| O61460 | vab-1 | Ephrin receptor 1 | Caenorhabditis elegans | SS |
| Q3E8W4 | ANX2 | Receptor-like protein kinase ANXUR2 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q8VZJ9 | CRCK2 | Calmodulin-binding receptor-like cytoplasmic kinase 2 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9FLW0 | At5g24010 | Probable receptor-like protein kinase At5g24010 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9LSV3 | WAKL16 | Putative wall-associated receptor kinase-like 16 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9M342 | WAKL15 | Wall-associated receptor kinase-like 15 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9M345 | LECRK42 | L-type lectin-domain containing receptor kinase IV.2 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9SCZ4 | FER | Receptor-like protein kinase FERONIA | Arabidopsis thaliana (Mouse-ear cress) | PR |
| O13147 | ephb3 | Ephrin type-B receptor 3 | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| O73878 | ephb4b | Ephrin type-B receptor 4b | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| O13146 | epha3 | Ephrin type-A receptor 3 | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MRGSGPRGAG | RRRTQGRGGG | GDTPRVPASL | AGCYSAPLKG | PLWTCLLLCA | ALRTLLASPS |
| 70 | 80 | 90 | 100 | 110 | 120 |
| NEVNLLDSRT | VLGDLGWIAF | PKNGWEEIGE | VDENYAPIHT | YQVCKVMEQN | QNNWLLTSWI |
| 130 | 140 | 150 | 160 | 170 | 180 |
| SNEGASRIFI | ELKFTLRDCN | SLPGGLGTCK | ETFNMYYFES | DDENGRNIKD | NQYIKIDTIA |
| 190 | 200 | 210 | 220 | 230 | 240 |
| ADESFTELDL | GDRVMKLNTE | VRDVGPLSKK | GFYLAFQDVG | ACIALVSVRV | YYKKCPSVVR |
| 250 | 260 | 270 | 280 | 290 | 300 |
| HLAVFPDTIT | GADSSQLLEV | SGSCVNHSVT | DDPPKMHCSA | EGEWLVPIGK | CMCKAGYEEK |
| 310 | 320 | 330 | 340 | 350 | 360 |
| NGTCQVCRPG | FFKASPHSQT | CSKCPPHSYT | HEEASTSCVC | EKDYFRRESD | PPTMACTRPP |
| 370 | 380 | 390 | 400 | 410 | 420 |
| SAPRNAISNV | NETSVFLEWI | PPADTGGGKD | VSYYILCKKC | NSHAGVCEEC | GGHVRYLPQQ |
| 430 | 440 | 450 | 460 | 470 | 480 |
| IGLKNTSVMM | ADPLAHTNYT | FEIEAVNGVS | DLSPGTRQYV | SVNVTTNQAA | PSPVTNVKKG |
| 490 | 500 | 510 | 520 | 530 | 540 |
| KIAKNSISLS | WQEPDRPNGI | ILEYEIKYFE | KDQETSYTII | KSKETTITAE | GLKPASVYVF |
| 550 | 560 | 570 | 580 | 590 | 600 |
| QIRARTAAGY | GVFSRRFEFE | TTPVFGASND | QSQIPIIGVS | VTVGVILLAV | MIGFLLSGSC |
| 610 | 620 | 630 | 640 | 650 | 660 |
| CECGCGRASS | LCAVAHPSLI | WRCGYSKAKQ | DPEEEKMHFH | NGHIKLPGVR | TYIDPHTYED |
| 670 | 680 | 690 | 700 | 710 | 720 |
| PTQAVHEFGK | EIEASCITIE | RVIGAGEFGE | VCSGRLKLPG | KRELPVATKT | LKVGYTEKQR |
| 730 | 740 | 750 | 760 | 770 | 780 |
| RDFLSEASIM | GQFDHPNIIH | LEGVVTKSKP | VMIVTEYMEN | GSLDTFLKKN | DGQFTVIQLV |
| 790 | 800 | 810 | 820 | 830 | 840 |
| GMLRGIAAGM | KYLSDMGYVH | RDLAARNILI | NSNLVCKVSD | FGLSRVLEDD | PEAAYTTRGG |
| 850 | 860 | 870 | 880 | 890 | 900 |
| KIPIRWTAPE | AIAFRKFTSA | SDVWSYGIVM | WEVVSYGERP | YWEMTNQDVI | KAVEEGYRLP |
| 910 | 920 | 930 | 940 | 950 | 960 |
| SPMDCPAALY | QLMLDCWQKD | RNSRPKFDDI | VNMLDKLIRN | PSSLKTLVNA | SSRVSTLLAE |
| 970 | 980 | 990 | 1000 | ||
| HGSLGSGAYR | SVGEWLEATK | MGRYTEIFME | NGYSSMDAVA | QVTLE |