P54351

SS
Gene name
Nsf2
Protein name
Vesicle-fusing ATPase 2
Names
N-ethylmaleimide-sensitive fusion protein 2, NEM-sensitive fusion protein 2, Vesicular-fusion protein NSF2, dNsf-2, NSF-2
Species
Drosophila melanogaster (Fruit fly)
KEGG Pathway
dme:Dmel_CG33101
EC number
3.6.4.6: Acting on ATP; involved in cellular and subcellular movement
Protein Class

Descriptions

NSF is a member of the type II AAA+ (ATPase associated with various cellular activities) family. Mutations within the N-D1 linker region may increase the basal activity of NSF.

Autoinhibitory domains (AIDs)

208-227 (N-D1 linker region) SS

Target domain

257-404 (D1 domain or ATPase domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P54351

Entry ID Method Resolution Chain Position Source
AF-P54351-F1 Predicted AlphaFoldDB

No variants for P54351

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P54351

No associated diseases with P54351

8 regional properties for P54351

Type Name Position InterPro Accession
domain CDC48, N-terminal subdomain 8 - 92 IPR003338
domain AAA+ ATPase domain 257 - 404 IPR003593-1
domain AAA+ ATPase domain 540 - 676 IPR003593-2
domain ATPase, AAA-type, core 261 - 401 IPR003959-1
domain ATPase, AAA-type, core 544 - 670 IPR003959-2
conserved_site ATPase, AAA-type, conserved site 372 - 390 IPR003960
domain CDC48, domain 2 117 - 188 IPR004201
domain AAA ATPase, AAA+ lid domain 428 - 464 IPR041569

Functions

Description
EC Number 3.6.4.6 Acting on ATP; involved in cellular and subcellular movement
Subcellular Localization
  • Cytoplasm
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
Golgi stack The set of thin, flattened membrane-bounded compartments, called cisternae, that form the central portion of the Golgi complex. The stack usually comprises cis, medial, and trans cisternae; the cis- and trans-Golgi networks are not considered part of the stack.
presynapse The part of a synapse that is part of the presynaptic cell.

3 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient.
metal ion binding Binding to a metal ion.

13 GO annotations of biological process

Name Definition
Golgi to plasma membrane protein transport The directed movement of proteins from the Golgi to the plasma membrane in transport vesicles that move from the trans-Golgi network to the plasma membrane.
Golgi vesicle docking The initial attachment of a Golgi transport vesicle membrane to a target membrane, mediated by proteins protruding from the membrane of the Golgi vesicle and the target membrane.
intra-Golgi vesicle-mediated transport The directed movement of substances within the Golgi, mediated by small transport vesicles. These either fuse with the cis-Golgi or with each other to form the membrane stacks known as the cis-Golgi reticulum (network).
liquid clearance, open tracheal system The clearance of liquid from the epithelial tubes of an open tracheal system, shortly before the emergence of the larva, to generate an air-filled tubule system.
neuromuscular synaptic transmission The process of synaptic transmission from a neuron to a muscle, across a synapse.
neurotransmitter secretion The regulated release of neurotransmitter from the presynapse into the synaptic cleft via calcium-regulated exocytosis during synaptic transmission.
open tracheal system development The process whose specific outcome is the progression of an open tracheal system over time, from its formation to the mature structure. An open tracheal system is a respiratory system, a branched network of epithelial tubes that supplies oxygen to target tissues via spiracles. An example of this is found in Drosophila melanogaster.
regulation of synaptic assembly at neuromuscular junction Any process that modulates the frequency, rate or extent of synaptic assembly at neuromuscular junctions.
SNARE complex disassembly The disaggregation of the SNARE protein complex into its constituent components. The SNARE complex is a protein complex involved in membrane fusion; a stable ternary complex consisting of a four-helix bundle, usually formed from one R-SNARE and three Q-SNAREs with an ionic layer sandwiched between hydrophobic layers.
synaptic vesicle priming A process that converts synaptic vesicles to a state of competence for calcium triggered fusion with the active zone membrane by bringing the two membranes into very close proximity. Priming typically (but not always) occurs after docking (Jahn and Fasshauer, 2012). Primed vesicles are also capable of spontaneously fusing with the active zone membrane.
terminal branching, open tracheal system Formation of terminal branches in the open tracheal system. These are long cytoplasmic extensions that form fine tubules that transport oxygen directly to the tissues. An example of the process is found in Drosophila melanogaster.
trachea morphogenesis The process in which a trachea is generated and organized. The trachea is the portion of the airway that attaches to the bronchi as it branches.
vesicle-mediated transport A cellular transport process in which transported substances are moved in membrane-bounded vesicles; transported substances are enclosed in the vesicle lumen or located in the vesicle membrane. The process begins with a step that directs a substance to the forming vesicle, and includes vesicle budding and coating. Vesicles are then targeted to, and fuse with, an acceptor membrane.

6 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P46461 comt Vesicle-fusing ATPase 1 Drosophila melanogaster (Fruit fly) SS
P46459 NSF Vesicle-fusing ATPase Homo sapiens (Human) SS
P46460 Nsf Vesicle-fusing ATPase Mus musculus (Mouse) SS
Q9QUL6 Nsf Vesicle-fusing ATPase Rattus norvegicus (Rat) SS
Q94392 nsf-1 Vesicle-fusing ATPase Caenorhabditis elegans SS
Q9M0Y8 NSF Vesicle-fusing ATPase Arabidopsis thaliana (Mouse-ear cress) SS
10 20 30 40 50 60
MSIEKAHRMR AIKCPTDELS LTNKAIVNVS DFTEEVKYVD ISPGPGLHYI FALEKISGPE
70 80 90 100 110 120
LPLGHVGFSL VQRKWATLSI NQEIDVRPYR FDASADIITL VSFETDFLQK KTTTQEPYDS
130 140 150 160 170 180
DEMAKEFLMQ FAGMPLTVGQ TLVFQFKDKK FLGLAVKTLE AVDPRTVGDS LPKTRNVRFG
190 200 210 220 230 240
RILGNTVVQF EKAENSVLNL QGRSKGKIVR QSIINPDWDF GKMGIGGLDK EFNAIFRRAF
250 260 270 280 290 300
ASRVFPPELV EQLGIKHVKG ILLYGPPGTG KTLMARQIGT MLNAREPKIV NGPQILDKYV
310 320 330 340 350 360
GESEANIRRL FAEAEEEEKR LGPNSGLHII IFDEIDAICK ARGSVAGNSG VHDTVVNQLL
370 380 390 400 410 420
AKIDGVEQLN NILVIGMTNR RDMIDEALLR PGRLEVQMEI SLPNEQGRVQ ILNIHTKRMR
430 440 450 460 470 480
DFNKIASDVD NNEIAAKTKN FSGAELEGLV RAAQSTAMNR LIKADSKVHV DPEAMEKLRV
490 500 510 520 530 540
TRADFLHALD NDIKPAFGAA QEMLENLLAR GIINWGPPVT ELLEDGMLSV QQAKATESSG
550 560 570 580 590 600
LVSVLIEGAP NSGKSALAAN LAQLSDFPFV KVCSPEDMVG FTESAKCLHI RKIFDDAYRS
610 620 630 640 650 660
TLSCIVVDNV ERLLDYGPIG PRYSNLTLQA LLVLLKKQPP KGRKLLILCT SSRRDVLEEM
670 680 690 700 710 720
EMLSAFTSVL HVSNLSTPEN VLAVLDDSDL FSPEELQSIA RKMAGKRLCI GIKKLLALID
730 740 750
MIRQSEPHQR VIKFLSKMEE EGGLEMDRVQ GH