P53894

SS
Gene name
CBK1 (YNL161W, N1727)
Protein name
Serine/threonine-protein kinase CBK1
Names
EC 2.7.11.1 , Cell wall biosynthesis kinase
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YNL161W
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

STK38, called nuclear Dbf2-related kinase 1 (NDR1 protein kinase), is a subclass of the AGC family of protein kinases that are involved in the regulation of cell division and cell morphology. NDR kinase activity is regulated by phosphorylation and by the Ca2+-binding protein, S100B. MOB1 also stimulates NDR kinase activity and interacts with NDR. <br> A common feature of the NDR family of kinases is an insert in the catalytic domain of 30 - 60 amino acids between subdomains VII and VIII. The insert sequence is not highly conserved but contains in all cases many positively charged amino acids. Mutations of the residues within the insert (autoinhibitory element) increase kinase activity and the binding of MOB1 or S100B to the N-terminal domain (SMA domain) of NDR induces the release of autoinhibition.

Autoinhibitory domains (AIDs)

554-565 (Autoinhibitory sequence) SS

Target domain

352-672 (Protein kinase domain)

Relief mechanism

Partner binding

Assay

Accessory elements

492-504 (Activation loop from InterPro)

Target domain

352-672 (Protein kinase domain)

Relief mechanism

Assay

567-576 (Activation loop from InterPro)

Target domain

352-672 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

6 structures for P53894

Entry ID Method Resolution Chain Position Source
4LQP X-ray 450 A A 251-756 PDB
4LQQ X-ray 360 A A/D 251-756 PDB
4LQS X-ray 330 A A 251-756 PDB
5NCL X-ray 315 A A 251-756 PDB
5NCM X-ray 280 A B 251-351 PDB
AF-P53894-F1 Predicted AlphaFoldDB

13 variants for P53894

Variant ID(s) Position Change Description Diseaes Association Provenance
s14-332615 6 T>S No SGRP
s14-332840 81 G>D No SGRP
s14-332896 100 Y>H No SGRP
s14-332945 116 G>D No SGRP
s14-332989 131 G>S No SGRP
s14-333252 218 Q>H No SGRP
s14-333254 219 L>H No SGRP
s14-333253 219 L>I No SGRP
s14-333262 222 Q>* No SGRP
s14-333297 233 H>Q No SGRP
s14-333314 239 Q>P No SGRP
s14-333389 264 N>T No SGRP
s14-334787 730 S>N No SGRP

No associated diseases with P53894

4 regional properties for P53894

Type Name Position InterPro Accession
domain Protein kinase domain 352 - 672 IPR000719
domain AGC-kinase, C-terminal 673 - 754 IPR000961
active_site Serine/threonine-protein kinase, active site 471 - 483 IPR008271
binding_site Protein kinase, ATP binding site 358 - 381 IPR017441

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

12 GO annotations of cellular component

Name Definition
actin cytoskeleton The part of the cytoskeleton (the internal framework of a cell) composed of actin and associated proteins. Includes actin cytoskeleton-associated complexes.
cell cortex The region of a cell that lies just beneath the plasma membrane and often, but not always, contains a network of actin filaments and associated proteins.
cellular bud A protuberance from a cell of an organism that reproduces by budding, which will grow larger and become a separate daughter cell after nuclear division, cytokinesis, and cell wall formation (when appropriate). The daughter cell may completely separate from the mother cell, or the mother and daughter cells may remain associated.
cellular bud neck The constriction between the mother cell and daughter cell (bud) in an organism that reproduces by budding.
cellular bud tip The end of a cellular bud distal to the site of attachment to the mother cell.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytoplasmic stress granule A dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress.
incipient cellular bud site The portion of the budding yeast plasma membrane where a daughter cell will emerge. The yeast marks this spot with bud-site selection proteins before bud emergence occurs. Actin is polarized to this spot just prior to and during bud emergence.
mating projection tip The apex of the mating projection in unicellular fungi exposed to mating pheromone; site of polarized growth.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
prospore membrane The prospore membrane is a double-membraned structure that extends from the cytoplasmic face of the spindle pole bodies to encompass the spindle pole bodies and the four nuclear lobes that are formed during meiosis. It helps isolate the meiotic nuclei from the cytoplasm during spore formation and serves as a foundation for the formation of the spore walls. An example of this component is found in Schizosaccharomyces pombe.
serine/threonine protein kinase complex A protein complex which is capable of protein serine/threonine kinase activity.

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
identical protein binding Binding to an identical protein or proteins.
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions

10 GO annotations of biological process

Name Definition
actomyosin structure organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures containing both actin and myosin or paramyosin. The myosin may be organized into filaments.
budding cell apical bud growth Growth at the tip of a bud, in a cell that reproduces by budding.
establishment or maintenance of actin cytoskeleton polarity Any cellular process that results in the specification, formation or maintenance of polarized actin-based cytoskeletal structures.
establishment or maintenance of cell polarity Any cellular process that results in the specification, formation or maintenance of anisotropic intracellular organization or cell growth patterns.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
mitotic cytokinesis A cell cycle process that results in the division of the cytoplasm of a cell after mitosis, resulting in the separation of the original cell into two daughter cells.
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
regulation of fungal-type cell wall organization Any process that modulates the rate, frequency or extent of the formation, arrangement of constituent parts, or disassembly of the fungal-type cell wall.
regulation of protein secretion Any process that modulates the frequency, rate or extent of the controlled release of a protein from a cell.
septum digestion after cytokinesis The process of physically separating the septal cell wall material by enzymatic digestion, that occurs after daughter cells are separated by cytokinesis.

9 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q03407 PKH1 Serine/threonine-protein kinase PKH1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
P24583 PKC1 Protein kinase C-like 1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SS
A2VDV2 STK38 Serine/threonine-protein kinase 38 Bos taurus (Bovine) SS
Q9NBK5 trc Serine/threonine-protein kinase tricornered Drosophila melanogaster (Fruit fly) SS
Q9Y2H1 STK38L Serine/threonine-protein kinase 38-like Homo sapiens (Human) EV
Q15208 STK38 Serine/threonine-protein kinase 38 Homo sapiens (Human) EV
Q7TSE6 Stk38l Serine/threonine-protein kinase 38-like Mus musculus (Mouse) SS
Q91VJ4 Stk38 Serine/threonine-protein kinase 38 Mus musculus (Mouse) SS
Q2L6W9 sax-1 Serine/threonine-protein kinase sax-1 Caenorhabditis elegans SS
10 20 30 40 50 60
MYNSSTNHHE GAPTSGHGYY MSQQQDQQHQ QQQQYANEMN PYQQIPRPPA AGFSSNYMKE
70 80 90 100 110 120
QGSHQSLQEH LQRETGNLGS GFTDVPALNY PATPPPHNNY AASNQMINTP PPSMGGLYRH
130 140 150 160 170 180
NNNSQSMVQN GNGSGNAQLP QLSPGQYSIE SEYNQNLNGS SSSSPFHQPQ TLRSNGSYSS
190 200 210 220 230 240
GLRSVKSFQR LQQEQENVQV QQQLSQAQQQ NSRQQQQQLQ YQQQQQQQQQ QQHMQIQQQQ
250 260 270 280 290 300
QQQQQQQQSQ SPVQSGFNNG TISNYMYFER RPDLLTKGTQ DKAAAVKLKI ENFYQSSVKY
310 320 330 340 350 360
AIERNERRVE LETELTSHNW SEERKSRQLS SLGKKESQFL RLRRTRLSLE DFHTVKVIGK
370 380 390 400 410 420
GAFGEVRLVQ KKDTGKIYAM KTLLKSEMYK KDQLAHVKAE RDVLAGSDSP WVVSLYYSFQ
430 440 450 460 470 480
DAQYLYLIME FLPGGDLMTM LIRWQLFTED VTRFYMAECI LAIETIHKLG FIHRDIKPDN
490 500 510 520 530 540
ILIDIRGHIK LSDFGLSTGF HKTHDSNYYK KLLQQDEATN GISKPGTYNA NTTDTANKRQ
550 560 570 580 590 600
TMVVDSISLT MSNRQQIQTW RKSRRLMAYS TVGTPDYIAP EIFLYQGYGQ ECDWWSLGAI
610 620 630 640 650 660
MYECLIGWPP FCSETPQETY RKIMNFEQTL QFPDDIHISY EAEDLIRRLL THADQRLGRH
670 680 690 700 710 720
GGADEIKSHP FFRGVDWNTI RQVEAPYIPK LSSITDTRFF PTDELENVPD SPAMAQAAKQ
730 740 750
REQMTKQGGS APVKEDLPFI GYTYSRFDYL TRKNAL