AiPD: Autoinhibited Protein Database

P53350

Gene name	PLK1 (PLK)
Protein name	Serine/threonine-protein kinase PLK1
Names	Polo-like kinase 1, PLK-1, Serine/threonine-protein kinase 13, STPK13
Species	Homo sapiens (Human)
KEGG Pathway	hsa:5347
EC number	2.7.11.21: Protein-serine/threonine kinases
Protein Class	SERINE/THREONINE-PROTEIN KINASE PLK (PTHR24345)

Descriptions

Polo-like kinase Plk1 acts at various stages in the cell cycle, specifically mitosis and cytokinesis. The kinase activity is regulated by a conserved C-terminal domain, termed the polo box domain (PBD). PBD is composed of the two polo boxes and an N-terminal extension, and each polo box contains a continuous six-stranded antiparallel β-sheet and an α-helix. The C-terminal region containing a polo-box domain is involved in autoinhibition via intramolecular interaction with the kinase domain in PLK1.

Autoinhibitory domains (AIDs)

Target domain	53-305 (Protein kinase domain)
Relief mechanism	PTM
Assay	Structural analysis

AID

367-603 (Polo box domain)

Target domain

53-305 (Protein kinase domain)

Relief mechanism

PTM (Phosphorylation at Thr-210 by the upstream kinase)

Assay

Structural analysis

Accessory elements

193-216 (Activation loop from InterPro)

Target domain	53-305 (Protein kinase domain)
Relief mechanism
Assay

References

Cheng KY et al. (2003) "The crystal structure of the human polo-like kinase-1 polo box domain and its phospho-peptide complex", The EMBO journal, 22, 5757-68

Zhu K et al. (2016) "Phospho-Pon Binding-Mediated Fine-Tuning of Plk1 Activity", Structure (London, England : 1993), 24, 1110-9

Autoinhibited structure

Activated structure

71 structures for P53350

Entry ID	Method	Resolution	Chain	Position	Source
1Q4K	X-ray	230 A	A/B/C	345-603	PDB
1Q4O	X-ray	220 A	A/B	367-603	PDB
1UMW	X-ray	190 A	A/B	367-603	PDB
2OGQ	X-ray	195 A	A	365-603	PDB
2OJX	X-ray	285 A	A	365-603	PDB
2OU7	X-ray	240 A	A	13-345	PDB
2OWB	X-ray	210 A	A	13-345	PDB
2RKU	X-ray	195 A	A	37-330	PDB
2V5Q	X-ray	230 A	A/B	33-345	PDB
2YAC	X-ray	220 A	A	36-345	PDB
3BZI	X-ray	210 A	A	365-603	PDB
3C5L	X-ray	233 A	A	373-593	PDB
3FC2	X-ray	245 A	A	13-345	PDB
3FVH	X-ray	158 A	A	371-603	PDB
3HIH	X-ray	170 A	A/B	371-593	PDB
3HIK	X-ray	177 A	A	367-603	PDB
3KB7	X-ray	250 A	A	36-345	PDB
3P2W	X-ray	166 A	A	371-594	PDB
3P2Z	X-ray	179 A	A	371-594	PDB
3P34	X-ray	140 A	A	371-594	PDB
3P35	X-ray	209 A	A/B/C	371-594	PDB
3P36	X-ray	159 A	A	371-594	PDB
3P37	X-ray	238 A	A/B/C	371-594	PDB
3Q1I	X-ray	140 A	A	371-594	PDB
3RQ7	X-ray	155 A	A	371-603	PDB
3THB	X-ray	250 A	A	13-345	PDB
4A4L	X-ray	235 A	A	36-345	PDB
4A4O	X-ray	270 A	A	36-345	PDB
4DFW	X-ray	155 A	A	367-603	PDB
4E67	X-ray	210 A	A	371-594	PDB
4E9C	X-ray	170 A	A	371-594	PDB
4E9D	X-ray	275 A	A	371-594	PDB
4H5X	X-ray	195 A	A/B	367-603	PDB
4H71	X-ray	193 A	A/B	367-603	PDB
4HAB	X-ray	265 A	A/B/C	371-593	PDB
4HCO	X-ray	275 A	A/B	367-603	PDB
4HY2	X-ray	200 A	A	371-595	PDB
4J52	X-ray	230 A	A	38-330	PDB
4J53	X-ray	250 A	A	38-330	PDB
4LKL	X-ray	158 A	A	372-593	PDB
4LKM	X-ray	200 A	A/C	371-601	PDB
4O56	X-ray	180 A	A	367-603	PDB
4O6W	X-ray	145 A	A	371-603	PDB
4O9W	X-ray	169 A	A	373-594	PDB
4RCP	X-ray	160 A	A	372-599	PDB
4WHH	X-ray	190 A	A	371-603	PDB
4WHK	X-ray	180 A	A	371-603	PDB
4WHL	X-ray	271 A	A	371-603	PDB
4X9R	X-ray	140 A	A	371-603	PDB
4X9V	X-ray	143 A	A	371-603	PDB
4X9W	X-ray	180 A	A	371-603	PDB
5J19	X-ray	200 A	A/B	367-594	PDB
5NEI	X-ray	268 A	A	371-603	PDB
5NFU	X-ray	181 A	A	371-602	PDB
5NJE	X-ray	198 A	A	371-603	PDB
5NMM	X-ray	202 A	A	371-603	PDB
5NN1	X-ray	178 A	A	371-603	PDB
5NN2	X-ray	181 A	A	371-594	PDB
5TA6	X-ray	250 A	A	13-345	PDB
5TA8	X-ray	260 A	A	13-345	PDB
6AX4	X-ray	145 A	A	371-603	PDB
6GY2	X-ray	311 A	A/B	365-603	PDB
7MSO	X-ray	185 A	A/B	371-603	PDB
7MX1	X-ray	164 A	A/B	371-603	PDB
8BJT	X-ray	219 A	A	37-330	PDB
8CRC	X-ray	165 A	A	371-594	PDB
8JOQ	X-ray	180 A	A	371-594	PDB
8JOY	X-ray	261 A	A	371-594	PDB
8X72	X-ray	220 A	A	13-345	PDB
8XB9	X-ray	195 A	A	371-603	PDB
AF-P53350-F1	Predicted				AlphaFoldDB

362 variants for P53350

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
CA7964124 rs759656440	4	A>S		No	ClinGen ExAC gnomAD
CA395148590 rs1290386708	5	V>M		No	ClinGen gnomAD
CA395148597 rs1423596323	6	T>A		No	ClinGen gnomAD
rs1021134870 CA279535578	7	A>S		No	ClinGen TOPMed
rs1245346767 CA395148610	8	G>E		No	ClinGen TOPMed
CA395148614 rs1438981347	9	K>E		No	ClinGen gnomAD
CA7964126 rs571545759	12	R>W		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs764367827 CA7964128	14	P>A		No	ClinGen ExAC gnomAD
CA7964129 rs753468980	15	A>T		No	ClinGen ExAC TOPMed gnomAD
CA395148651 rs1222018262	15	A>V		No	ClinGen gnomAD
rs1290770914 CA395148661	17	P>S		No	ClinGen gnomAD
rs1453123013 CA395148672	19	K>E		No	ClinGen gnomAD
CA279535590 rs966462064	19	K>R		No	ClinGen TOPMed gnomAD
rs1232261739 CA395148683	20	A>G		No	ClinGen gnomAD
rs765005937 CA7964131	21	G>V		No	ClinGen ExAC
rs1382853622 CA395148690	22	V>I		No	ClinGen gnomAD
rs749921244 CA7964132	25	V>I		No	ClinGen ExAC TOPMed gnomAD
rs758028407 CA7964133	26	A>T		No	ClinGen ExAC TOPMed gnomAD
CA7964134 rs780089094	27	A>P		No	ClinGen ExAC TOPMed gnomAD
rs1315465388 CA395148721	27	A>V		No	ClinGen TOPMed
rs754746085 CA7964136	28	P>A		No	ClinGen ExAC TOPMed gnomAD
rs748043179 CA7964138	28	P>L		No	ClinGen ExAC TOPMed gnomAD
rs754746085 CA7964137	28	P>S		No	ClinGen ExAC TOPMed gnomAD
CA7964139 rs771030731	30	A>T		No	ClinGen ExAC TOPMed gnomAD
rs1380681511 CA395148736	30	A>V		No	ClinGen gnomAD
rs933156352 CA279535613	32	A>V		No	ClinGen TOPMed
rs774608785 CA7964140	33	A>V		No	ClinGen ExAC gnomAD
rs1338733957 CA395148753	34	A>T		No	ClinGen gnomAD
rs1212626444 CA395148764	35	P>L		No	ClinGen TOPMed gnomAD
CA279535620 rs983282909	36	P>L		No	ClinGen TOPMed
CA7964141 rs745902058	36	P>T		No	ClinGen ExAC TOPMed gnomAD
CA395148789 rs1260213034	39	E>D		No	ClinGen TOPMed
CA395148800 rs1241134783	41	P>S		No	ClinGen TOPMed gnomAD
CA395148798 rs1241134783	41	P>T		No	ClinGen TOPMed gnomAD
RCV001250902 rs1597133306	43	V>A		No	ClinVar dbSNP
rs1597133306 CA395148817	43	V>G		No	ClinGen Ensembl
rs1597133314 CA395148821	44	L>P		No	ClinGen Ensembl
rs1474208424 CA395148842	47	P>L		No	ClinGen gnomAD
CA395148839 rs1369881552	47	P>S		No	ClinGen TOPMed gnomAD
CA395148845 rs1423348958	48	R>C		No	ClinGen gnomAD
rs764772076 CA7964148	50	R>G		No	ClinGen ExAC TOPMed gnomAD
CA395148858 rs1404475875	50	R>Q		No	ClinGen gnomAD
rs750121456 CA7964149	52	R>H		No	ClinGen ExAC gnomAD
rs984792608 CA279535633	55	R>W		No	ClinGen Ensembl
rs1342552399 CA395148891	56	G>S		No	ClinGen gnomAD
CA395148896 rs757915883	57	R>C		No	ClinGen ExAC gnomAD
CA7964150 rs757915883	57	R>S		No	ClinGen ExAC gnomAD
CA395148920 rs1318356215	60	G>V		No	ClinGen gnomAD
rs757818441 CA7964154	69	E>Q		No	ClinGen ExAC gnomAD
CA395148996 rs1257642556	71	S>L		No	ClinGen gnomAD
rs371297722 CA7964156	72	D>E		No	ClinGen ESP ExAC TOPMed gnomAD
rs1382720064 CA395149004	72	D>V		No	ClinGen TOPMed
rs1302599020 CA395149012	74	D>N		No	ClinGen TOPMed
rs755962137 CA7964157	76	K>N		No	ClinGen ExAC gnomAD
CA395149046 rs1597133406	78	V>G		No	ClinGen Ensembl
rs1196244303 CA395149061	81	G>S		No	ClinGen gnomAD
CA395149079 rs1429440231	83	I>S		No	ClinGen TOPMed
CA7964158 rs779069738	83	I>V		No	ClinGen ExAC TOPMed gnomAD
rs746091320 CA7964159	84	V>M		No	ClinGen ExAC gnomAD
CA395149086 rs1371715205	85	P>A		No	ClinGen TOPMed
CA395149100 rs1404452752	87	S>A		No	ClinGen gnomAD
CA395149126 rs1429623555	91	K>R		No	ClinGen TOPMed
rs747176994 CA7964163	92	P>A		No	ClinGen ExAC TOPMed gnomAD
CA395149133 rs1597133437	92	P>L		No	ClinGen Ensembl
CA7964165 rs777243517	93	H>D		No	ClinGen ExAC gnomAD
CA7964166 rs762032549	93	H>P		No	ClinGen ExAC gnomAD
CA7964167 rs770312220	93	H>Q		No	ClinGen ExAC gnomAD
rs202000368 CA279535679	98	M>I		No	ClinGen gnomAD
rs1203983274 CA395149185	99	S>F		No	ClinGen gnomAD
CA395149212 rs1187983580	103	S>T		No	ClinGen TOPMed gnomAD
CA395149217 rs1269101759	104	I>V		No	ClinGen TOPMed
CA395149237 rs1489046989	107	S>C		No	ClinGen gnomAD
CA7964170 rs139594215	107	S>N		No	ClinGen ESP ExAC TOPMed gnomAD
CA395149238 rs1489046989	107	S>R		No	ClinGen gnomAD
CA7964172 rs759137799	109	A>V		No	ClinGen ExAC gnomAD
CA7964174 rs752714699	110	H>N		No	ClinGen ExAC gnomAD
CA395149277 rs755802528	112	H>Q		No	ClinGen ExAC TOPMed gnomAD
CA395149278 rs1311515674	113	V>I		No	ClinGen TOPMed
rs1221722889 CA395149285	114	V>L		No	ClinGen TOPMed
CA395149313 rs1158092975	118	G>S		No	ClinGen gnomAD
CA395149354 rs1391027910	123	N>S		No	ClinGen TOPMed
rs1387078647 CA395149372	125	F>L		No	ClinGen gnomAD
rs780291064 CA7964179	128	V>A		No	ClinGen ExAC gnomAD
CA279535699 rs933521218	129	V>M		No	ClinGen gnomAD
CA7964180 rs747036711	134	R>C		No	ClinGen ExAC gnomAD
rs1270146071 CA395149433	135	R>Q		No	ClinGen gnomAD
rs768801966 CA7964181	135	R>W		No	ClinGen ExAC gnomAD
CA7964215 rs750244892	143	K>R		No	ClinGen ExAC gnomAD
CA279535965 rs751015580	147	A>S		No	ClinGen gnomAD
CA395149523 rs751015580	147	A>T		No	ClinGen gnomAD
CA395149538 rs1423069231	149	T>I		No	ClinGen TOPMed
CA7964217 rs767675632	151	P>A		No	ClinGen ExAC gnomAD
CA395149567 rs1217147797	154	R>*		No	ClinGen gnomAD
CA7964218 rs536418824	154	R>Q		No	ClinGen 1000Genomes ExAC gnomAD
rs1597133850 CA395149581	156	Y>S		No	ClinGen Ensembl
rs1387460801 CA395149592	158	R>Q		No	ClinGen TOPMed
CA7964219 rs756287352	158	R>W		No	ClinGen ExAC gnomAD
CA395149600 rs1166017820	159	Q>L		No	ClinGen TOPMed
rs934973222 CA279535978	160	I>T		No	ClinGen gnomAD
rs1396712472 CA395149611	161	V>L		No	ClinGen gnomAD
CA395149616 rs1428414613	162	L>F		No	ClinGen TOPMed
rs1156269385 CA395149639	165	Q>R		No	ClinGen gnomAD
CA395149666 rs1597133883 COSM167619	169	R>Q	large_intestine [Cosmic]	No	ClinGen cosmic curated Ensembl
rs1052549183 CA279535983	171	R>L		No	ClinGen gnomAD
rs1052549183 CA395149679	171	R>Q		No	ClinGen gnomAD
rs1436785460 CA395149683	172	V>F		No	ClinGen gnomAD
rs1436785460 CA395149681	172	V>I		No	ClinGen gnomAD
rs1434706059 CA395149689	173	I>V		No	ClinGen gnomAD
CA7964224 rs779319154	179	L>V		No	ClinGen ExAC gnomAD
rs746280506 CA7964225	183	F>L		No	ClinGen ExAC
rs1044876624 CA279535994	184	L>M		No	ClinGen Ensembl
CA7964226 rs771722070	185	N>S		No	ClinGen ExAC gnomAD
rs775051167 CA7964227	187	D>E		No	ClinGen ExAC TOPMed gnomAD
rs375744773 CA7964238	195	F>L		No	ClinGen ESP ExAC gnomAD
CA7964239 rs754032412	199	T>A		No	ClinGen ExAC TOPMed gnomAD
CA395149878 rs1166091191	200	K>T		No	ClinGen TOPMed
CA7964241 rs140934881	202	E>K		No	ClinGen ESP ExAC TOPMed gnomAD
rs758709314 CA7964243	204	D>G		No	ClinGen ExAC gnomAD
CA7964245 rs779641699	205	G>R		No	ClinGen ExAC TOPMed gnomAD
CA7964244 rs779641699	205	G>W		No	ClinGen ExAC TOPMed gnomAD
CA395149922 rs1235432057	206	E>D		No	ClinGen TOPMed gnomAD
rs1414986698 CA395149974	214	T>I		No	ClinGen gnomAD
CA7964250 rs369625624	224	S>N		No	ClinGen ESP ExAC TOPMed gnomAD
rs11645497 CA7964252 COSM329545	230	F>L	haematopoietic_and_lymphoid_tissue [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed gnomAD
CA279536244 rs201113590	234	V>M		No	ClinGen 1000Genomes gnomAD
rs572490408 CA7964254	241	M>T		No	ClinGen ExAC gnomAD
CA279536606 rs866571631	247	G>D		No	ClinGen gnomAD
rs751899280 CA7964281	249	P>L		No	ClinGen ExAC gnomAD
CA395150232 rs1567239041	250	P>S		No	ClinGen Ensembl
rs1321541989 CA395150269	255	C>Y		No	ClinGen gnomAD
VAR_041019 CA7964284 rs35056440	261	L>F		No	ClinGen UniProt 1000Genomes ESP ExAC TOPMed dbSNP gnomAD
rs1351133950 CA395150314	262	R>L		No	ClinGen TOPMed gnomAD
rs1351133950 COSM1323646 CA395150313	262	R>Q	ovary [Cosmic]	No	ClinGen cosmic curated TOPMed gnomAD
rs148585508 CA7964285	262	R>W		No	ClinGen ESP ExAC TOPMed gnomAD
CA7964288 rs376917678	265	K>R		No	ClinGen ESP ExAC TOPMed gnomAD
rs1207220606 CA395150363	269	S>G		No	ClinGen gnomAD
CA7964289 rs779038687	270	I>V		No	ClinGen ExAC gnomAD
rs753166578 CA7964303	274	I>V		No	ClinGen ExAC TOPMed gnomAD
rs539361988 CA7964304	275	N>K		No	ClinGen 1000Genomes ExAC gnomAD
CA395150647 rs1597135198	275	N>T		No	ClinGen Ensembl
CA7964306 rs753261853	277	V>M		No	ClinGen ExAC TOPMed gnomAD
CA7964307 rs756845433	278	A>G		No	ClinGen ExAC
CA395150662 rs759970852	278	A>S		No	ClinGen gnomAD
CA279539802 rs759970852	278	A>T		No	ClinGen gnomAD
CA395150667 rs745801697	279	A>S		No	ClinGen ExAC TOPMed gnomAD
rs745801697 CA7964309	279	A>T		No	ClinGen ExAC TOPMed gnomAD
rs746888240 CA7964312	282	I>M		No	ClinGen ExAC TOPMed gnomAD
CA7964311 rs779699124	282	I>V		No	ClinGen ExAC TOPMed gnomAD
CA279539815 rs374590367	283	Q>H		No	ClinGen ESP TOPMed
CA7964313 rs769874912	286	L>P		No	ClinGen ExAC gnomAD
rs769874912 CA279539816	286	L>R		No	ClinGen ExAC gnomAD
rs1311497324 CA395150762	288	T>I		No	ClinGen TOPMed gnomAD
rs765747258 CA7964314	291	T>I		No	ClinGen ExAC TOPMed gnomAD
rs1275646491 CA395150793	292	A>S		No	ClinGen gnomAD
CA395150798 rs1397343920	292	A>V		No	ClinGen gnomAD
CA279539827 rs1041097622 COSM1518694	293	R>H	lung [Cosmic]	No	ClinGen cosmic curated TOPMed
rs753048559 CA7964317	294	P>L		No	ClinGen ExAC gnomAD
CA395150820 rs1274670002	295	T>I		No	ClinGen gnomAD
rs146543273 CA7964318	296	I>V		No	ClinGen ESP ExAC TOPMed gnomAD
VAR_051659 rs16972799 CA7964319	297	N>D		No	ClinGen UniProt 1000Genomes ESP ExAC TOPMed dbSNP gnomAD
CA7964320 rs775691254	297	N>S		No	ClinGen ExAC TOPMed gnomAD
CA7964321 rs761146699 COSM1262522	298	E>K	oesophagus [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed gnomAD
rs763601917 CA7964322	301	N>S		No	ClinGen ExAC gnomAD
rs1477452628 CA395150903	303	E>K		No	ClinGen gnomAD
CA7964324 rs761337968	308	G>C		No	ClinGen ExAC gnomAD
rs764851103 CA7964325	310	I>T		No	ClinGen ExAC TOPMed gnomAD
rs145066711 CA7964328	313	R>C		No	ClinGen ESP ExAC TOPMed gnomAD
rs146806568 CA7964329 COSM557135	313	R>H	lung large_intestine [Cosmic]	No	ClinGen cosmic curated ESP ExAC TOPMed gnomAD
CA7964330 rs754834039	316	I>V		No	ClinGen ExAC gnomAD
rs1597135293 CA395151055	317	T>P		No	ClinGen Ensembl
rs1224974758 CA395151085	320	T>I		No	ClinGen TOPMed
rs1302143812 CA395151087	321	I>V		No	ClinGen Ensembl
CA7964332 rs749527952 COSM171762	326	S>L	large_intestine skin [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
rs199862442 CA7964334	327	I>T		No	ClinGen ESP ExAC TOPMed gnomAD
CA7964335 rs575376447	328	A>P		No	ClinGen 1000Genomes ExAC gnomAD
CA395151134 rs1440159569	328	A>V		No	ClinGen TOPMed
rs1452140119 CA395151138	329	P>S		No	ClinGen TOPMed gnomAD
rs752011535 CA279539886	330	S>G		No	ClinGen TOPMed
CA7964338 rs146042616	331	S>N		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA7964337 rs146042616	331	S>T		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
VAR_041020 CA7964339 rs45489499	332	L>V		No	ClinGen UniProt ESP ExAC dbSNP gnomAD
rs1260771202 CA395151162	333	D>N		No	ClinGen TOPMed gnomAD
CA7964342 rs765047496	335	S>R		No	ClinGen ExAC gnomAD
CA7964341 rs761542345	335	S>R		No	ClinGen ExAC TOPMed gnomAD
CA279539907 rs781498914	337	R>Q		No	ClinGen Ensembl
rs150139246 CA279539904	337	R>W		No	ClinGen ESP ExAC TOPMed gnomAD
rs772412347 CA7964344	339	P>A		No	ClinGen ExAC gnomAD
rs1487608227 CA395151204	340	L>F		No	ClinGen gnomAD
rs1023741689 CA279539936	344	N>D		No	ClinGen TOPMed
rs1436190197 CA395151231	344	N>S		No	ClinGen gnomAD
CA7964346 rs533321688	345	K>E		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs752470218 CA7964369	348	E>G		No	ClinGen ExAC gnomAD
CA279541472 rs755929210	349	N>K		No	ClinGen ExAC TOPMed gnomAD
CA279541478 rs771753889	350	P>A		No	ClinGen TOPMed
CA395151282 rs771753889	350	P>S		No	ClinGen TOPMed
rs1319627345 CA395151286	351	L>M		No	ClinGen TOPMed gnomAD
rs765399402 CA7964371	352	P>L		No	ClinGen ExAC gnomAD
rs374942700 CA7964372	354	R>C		No	ClinGen ESP ExAC TOPMed gnomAD
CA7964373 rs138670004	354	R>H		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs138670004 CA7964374	354	R>L		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs374942700 CA395151305	354	R>S		No	ClinGen ESP ExAC TOPMed gnomAD
rs747122668 CA7964375	355	P>L		No	ClinGen ExAC TOPMed gnomAD
rs781776740 CA7964377	356	R>P		No	ClinGen ExAC TOPMed gnomAD
rs781776740 CA7964378	356	R>Q		No	ClinGen ExAC TOPMed gnomAD
CA7964376 rs373003785	356	R>W		No	ClinGen ESP ExAC TOPMed gnomAD
CA7964379 rs770287669	358	K>E		No	ClinGen ExAC TOPMed gnomAD
rs772943552 CA7964380	358	K>R		No	ClinGen ExAC gnomAD
rs748958860 CA7964381	361	P>S		No	ClinGen ExAC TOPMed gnomAD
rs1307745072 CA395151353	362	V>G		No	ClinGen TOPMed
rs770558489 CA7964382	362	V>L		No	ClinGen ExAC gnomAD
CA7964383 rs773883513	364	R>G		No	ClinGen ExAC gnomAD
rs149319665 CA7964384	364	R>Q		No	ClinGen ESP ExAC TOPMed gnomAD
CA395151372 rs1221439433	366	T>A		No	ClinGen TOPMed
CA395151376 rs1376532841	366	T>I		No	ClinGen gnomAD
CA395151396 rs1597136540	369	V>G		No	ClinGen Ensembl
rs1227712165 CA395151401	370	V>A		No	ClinGen TOPMed gnomAD
rs1227712165 CA395151402	370	V>G		No	ClinGen TOPMed gnomAD
rs577937163 CA7964387	371	D>N		No	ClinGen ExAC TOPMed gnomAD
rs1273778563 CA395151414	372	C>S		No	ClinGen TOPMed gnomAD
rs1273778563 CA395151413	372	C>Y		No	ClinGen TOPMed gnomAD
CA395151421 rs1336368353	373	H>R		No	ClinGen TOPMed
CA7964389 rs531192640	373	H>Y		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs1468560703 CA395151434	375	S>N		No	ClinGen TOPMed
rs919194931 CA279541545	376	D>E		No	ClinGen TOPMed gnomAD
CA7964390 rs758643617	377	M>I		No	ClinGen ExAC gnomAD
CA395151482 rs1206210323	382	H>Y		No	ClinGen gnomAD
rs137928994 CA7964391	383	S>G		No	ClinGen ESP ExAC TOPMed gnomAD
CA395151506 rs1405110181	385	N>K		No	ClinGen TOPMed
rs755036110 CA7964393	385	N>S		No	ClinGen ExAC
CA395151513 rs1187655104	386	A>V		No	ClinGen TOPMed gnomAD
CA395151516 rs1419285399	387	S>F		No	ClinGen gnomAD
rs994653073 CA279541558	387	S>T		No	ClinGen TOPMed
CA395151522 rs1476473248	388	K>T		No	ClinGen gnomAD
rs1242105455 CA395151529	389	P>S		No	ClinGen TOPMed
rs200843318 CA7964394	390	S>L		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs756741138 CA7964396	392	R>C		No	ClinGen ExAC gnomAD
CA7964397 rs778276138	392	R>H		No	ClinGen ExAC TOPMed gnomAD
rs756741138 CA395151546	392	R>S		No	ClinGen ExAC gnomAD
CA395151576 rs1447076591	397	Q>*		No	ClinGen gnomAD
CA7964424 rs746563979	400	A>T		No	ClinGen ExAC TOPMed gnomAD
rs201491344 CA7964425	404	A>S		No	ClinGen ESP ExAC TOPMed gnomAD
rs1448067540 CA395151647	405	C>Y		No	ClinGen gnomAD
rs565735478 CA7964426	406	I>F		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA395151657 rs1567240498	407	P>A		No	ClinGen Ensembl
CA395151663 rs1181495539	408	I>V		No	ClinGen TOPMed
CA279542136 rs1019188887	421	Y>D		No	ClinGen Ensembl
rs762224700 CA7964454	432	V>M		No	ClinGen ExAC TOPMed gnomAD
rs758785599 CA7964457	434	V>L		No	ClinGen ExAC gnomAD
rs765976859 CA7964458	437	N>S		No	ClinGen ExAC TOPMed gnomAD
CA395151905 COSM1221199 rs1227390743	440	T>A	large_intestine [Cosmic]	No	ClinGen cosmic curated TOPMed
CA395151912 rs1353944469	441	R>C		No	ClinGen TOPMed
CA7964461 rs35347525	441	R>H		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA7964464 rs777872494	443	I>V		No	ClinGen ExAC gnomAD
CA7964465 rs749048434	444	L>F		No	ClinGen ExAC gnomAD
rs770851479 CA7964466	445	Y>C		No	ClinGen ExAC gnomAD
CA395151932 rs1174025932	445	Y>H		No	ClinGen gnomAD
CA395151973 rs1393559854	450	S>T		No	ClinGen gnomAD
CA395152001 rs1311438943	454	I>T		No	ClinGen gnomAD
rs767455739 CA279542562	454	I>V		No	ClinGen Ensembl
rs747225202 CA7964468	455	E>Q		No	ClinGen ExAC gnomAD
CA7964471 rs189573198	456	R>C		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs199640055 CA7964472	456	R>H		No	ClinGen 1000Genomes ExAC gnomAD
rs189573198 CA7964470	456	R>S		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA7964474 rs369087739	458	G>C		No	ClinGen ESP ExAC TOPMed gnomAD
CA395152022 rs369087739	458	G>R		No	ClinGen ESP ExAC TOPMed gnomAD
CA7964475 rs369087739	458	G>S		No	ClinGen ESP ExAC TOPMed gnomAD
CA279542598 rs893016241	461	S>T		No	ClinGen gnomAD
CA7964479 rs752098841	461	S>Y		No	ClinGen ExAC TOPMed gnomAD
rs45569335 VAR_041021 CA7964481	463	L>H		No	ClinGen UniProt 1000Genomes ESP ExAC TOPMed dbSNP gnomAD
CA395152062 rs1423531067	464	T>I		No	ClinGen TOPMed
rs757326389 CA7964483	465	V>M		No	ClinGen ExAC TOPMed gnomAD
CA395152093 rs1172564502	469	P>R		No	ClinGen gnomAD
rs571020158 CA7964484	469	P>S		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs184851025 CA7964514	476	I>M		No	ClinGen 1000Genomes ExAC gnomAD
rs1253309078 CA395152490	476	I>V		No	ClinGen gnomAD
rs775771251 CA7964515	483	R>C		No	ClinGen ExAC TOPMed gnomAD
rs760374318 CA7964516	483	R>H		No	ClinGen ExAC TOPMed gnomAD
rs1347432817 CA395152548	484	N>K		No	ClinGen TOPMed gnomAD
CA395152572 rs753480677	487	S>R		No	ClinGen gnomAD
rs763884087 CA7964517	488	E>K		No	ClinGen ExAC gnomAD
CA7964519 rs150875727	498	T>K		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs150875727 CA7964520	498	T>M		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA7964522 rs138397393	499	P>L		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA395152653 rs1309116201	500	R>C		No	ClinGen gnomAD
CA7964524 rs751350031	500	R>H		No	ClinGen ExAC TOPMed gnomAD
CA279542762 rs1054240818	501	E>*		No	ClinGen Ensembl
rs985550581 CA279542767	501	E>G		No	ClinGen TOPMed gnomAD
rs914374826 CA279542770	505	L>F		No	ClinGen TOPMed gnomAD
rs1231915356 CA395152691	506	A>T		No	ClinGen gnomAD
CA7964527 rs749324461	507	R>W		No	ClinGen ExAC gnomAD
rs945867732 CA279542776	509	P>L		No	ClinGen Ensembl
rs1310479212 COSM3387312 CA395152721	511	L>I	pancreas [Cosmic]	No	ClinGen cosmic curated TOPMed
rs149623133 CA7964529	512	R>Q		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs757330190 CA7964528	512	R>W		No	ClinGen ExAC TOPMed gnomAD
rs1353221013 CA395152734	513	T>I		No	ClinGen TOPMed
rs1418691449 CA395152729	513	T>S		No	ClinGen gnomAD
rs1442748968 CA395152740	514	W>S		No	ClinGen gnomAD
rs972715628 CA279542787	518	R>C		No	ClinGen TOPMed gnomAD
CA7964530 rs56027600 VAR_041022	518	R>H		No	ClinGen UniProt ESP ExAC TOPMed dbSNP gnomAD
COSM968839 CA7964532 rs775897156	520	A>T	endometrium [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
rs1299669276 CA395152795	523	L>M		No	ClinGen gnomAD
rs1353262765 CA395152802	524	H>Y		No	ClinGen TOPMed gnomAD
CA7964533 rs747431571	525	L>F		No	ClinGen ExAC gnomAD
CA395152820 rs1336351584	526	S>R		No	ClinGen gnomAD
rs1438034094 CA395152832	528	G>D		No	ClinGen gnomAD
rs1271912815 CA395152829	528	G>S		No	ClinGen TOPMed gnomAD
rs140033760 CA7964536	530	V>M		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA395152867 rs1187901305	533	N>S		No	ClinGen TOPMed gnomAD
CA395152927 rs1476815093	539	T>S		No	ClinGen TOPMed
rs376644188 CA7964560	543	L>F		No	ClinGen ESP ExAC gnomAD
rs1347511762 CA395152984	548	A>T		No	ClinGen gnomAD
CA7964562 rs752551627	550	V>M		No	ClinGen ExAC TOPMed gnomAD
rs1189706032 CA395153002	551	T>A		No	ClinGen TOPMed
rs757466531 CA7964563	553	I>L		No	ClinGen ExAC gnomAD
CA279542977 rs977271413	554	D>G		No	ClinGen gnomAD
CA7964565 rs371534600	554	D>N		No	ClinGen ESP ExAC TOPMed gnomAD
CA7964567 rs780546109	557	R>Q		No	ClinGen ExAC gnomAD
CA395153044 rs1379177197	557	R>W		No	ClinGen gnomAD
rs1260623231 CA395153063	560	R>C		No	ClinGen TOPMed
rs143546758 CA7964568	560	R>H		No	ClinGen ESP ExAC TOPMed gnomAD
CA395153070 rs1385549091	561	T>S		No	ClinGen gnomAD
rs1323299411 CA395153083 COSM1221200	563	R>C	large_intestine [Cosmic]	No	ClinGen cosmic curated TOPMed gnomAD
rs748564084 CA7964571 COSM267196	563	R>H	large_intestine [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed gnomAD
rs1048434068 CA279543012	566	L>I		No	ClinGen TOPMed gnomAD
rs887178996 CA279543020	570	Y>H		No	ClinGen TOPMed gnomAD
rs1210743504 CA395153149	573	C>S		No	ClinGen gnomAD
rs1271355789 CA395153156	574	K>E		No	ClinGen gnomAD
rs770360171 CA395153161	574	K>N		No	ClinGen ExAC TOPMed gnomAD
CA7964575 rs770360171	574	K>N		No	ClinGen ExAC TOPMed gnomAD
CA7964577 rs759460218	575	E>*		No	ClinGen ExAC gnomAD
CA7964578 rs772035502	575	E>V		No	ClinGen ExAC gnomAD
rs1435911732 CA395153174	577	A>P		No	ClinGen TOPMed gnomAD
CA395153173 rs1435911732	577	A>T		No	ClinGen TOPMed gnomAD
CA7964580 rs536981666	578	S>T		No	ClinGen 1000Genomes ExAC gnomAD
CA279543049 rs984791278 COSM1221202	579	R>Q	large_intestine [Cosmic]	No	ClinGen cosmic curated TOPMed gnomAD
CA7964581 rs765463407	579	R>W		No	ClinGen ExAC gnomAD
CA7964583 rs34359215	581	R>C		No	ClinGen ExAC TOPMed gnomAD
rs755512074 CA395153209	583	A>S		No	ClinGen ExAC TOPMed gnomAD
rs755512074 CA7964586	583	A>T		No	ClinGen ExAC TOPMed gnomAD
CA7964588 rs753069463	584	R>C		No	ClinGen ExAC TOPMed gnomAD
rs573637165 CA7964589	584	R>H		No	ClinGen ExAC gnomAD
rs1291934300 CA395153227	586	M>T		No	ClinGen TOPMed gnomAD
CA395153224 rs1208449375	586	M>V		No	ClinGen TOPMed gnomAD
CA395153232 rs1334309293	587	V>M		No	ClinGen TOPMed gnomAD
rs148952170 CA7964590	588	D>E		No	ClinGen ESP ExAC TOPMed gnomAD
CA7964592 rs770679580	593	S>P		No	ClinGen ExAC
CA7964593 rs778360538	594	R>C		No	ClinGen ExAC TOPMed gnomAD
CA279543126 rs1021332654	594	R>H		No	ClinGen TOPMed gnomAD
rs1021332654 CA395153278	594	R>L		No	ClinGen TOPMed gnomAD
CA279543118 rs778360538	594	R>S		No	ClinGen ExAC TOPMed gnomAD
VAR_051660 COSM702964 CA7964594 rs34001032	595	S>L	lung [Cosmic]	No	ClinGen cosmic curated UniProt ESP ExAC TOPMed dbSNP gnomAD
rs1329379434 CA395153280	595	S>P		No	ClinGen TOPMed
CA395153293 rs1256684214	597	S>N		No	ClinGen gnomAD
CA395153302 rs1359233506	598	N>S		No	ClinGen Ensembl
CA7964596 rs775440787	599	R>C		No	ClinGen ExAC TOPMed gnomAD
rs34954545 CA7964597 VAR_051661	599	R>H		No	ClinGen UniProt 1000Genomes ESP ExAC TOPMed dbSNP gnomAD
rs34954545 CA395153308	599	R>P		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs776547432 CA7964599	601	K>T		No	ClinGen ExAC gnomAD
CA279543166 rs763081575	602	A>P		No	ClinGen ExAC TOPMed gnomAD
rs763081575 CA395153323	602	A>S		No	ClinGen ExAC TOPMed gnomAD
rs763081575 CA7964600	602	A>T		No	ClinGen ExAC TOPMed gnomAD
rs766749879 CA7964601	602	A>V		No	ClinGen ExAC

1 associated diseases with P53350

Without disease ID

3 regional properties for P53350

Type	Name	Position	InterPro Accession
domain	PDZ domain	14 - 94	IPR001478-1
domain	PDZ domain	154 - 234	IPR001478-2
domain	EBP50, C-terminal	235 - 358	IPR015098

Functions

		Description
EC Number	2.7.11.21	Protein-serine/threonine kinases
Subcellular Localization	Nucleus Chromosome, centromere, kinetochore Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, spindle Midbody	localization at the centrosome starts at the G1/S transition (PubMed:24018379) During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores Localizes to the outer kinetochore Presence of SGO1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization Localizes onto the central spindle by phosphorylating and docking at midzone proteins KIF20A/MKLP2 and PRC1 Colocalizes with FRY to separating centrosomes and spindle poles from prophase to metaphase in mitosis, but not in other stages of the cell cycle Localization to the centrosome is required for S phase progression (PubMed:24018379) Colocalizes with HSF1 at the spindle poles during prometaphase (PubMed:18794143)
PANTHER Family	PTHR24345	SERINE/THREONINE-PROTEIN KINASE PLK
PANTHER Subfamily	PTHR24345:SF0	SERINE_THREONINE-PROTEIN KINASE PLK1
PANTHER Protein Class	non-receptor serine/threonine protein kinase protein modifying enzyme
PANTHER Pathway Category	No pathway information available

17 GO annotations of cellular component

Name	Definition
centriolar satellite	A small (70-100 nm) cytoplasmic granule that contains a number of centrosomal proteins; centriolar satellites traffic toward microtubule minus ends and are enriched near the centrosome.
centriole	A cellular organelle, found close to the nucleus in many eukaryotic cells, consisting of a small cylinder with microtubular walls, 300-500 nm long and 150-250 nm in diameter. It contains nine short, parallel, peripheral microtubular fibrils, each fibril consisting of one complete microtubule fused to two incomplete microtubules. Cells usually have two centrioles, lying at right angles to each other. At division, each pair of centrioles generates another pair and the twin pairs form the pole of the mitotic spindle.
centrosome	A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
chromatin	The ordered and organized complex of DNA, protein, and sometimes RNA, that forms the chromosome.
cytoplasm	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
kinetochore	A multisubunit complex that is located at the centromeric region of DNA and provides an attachment point for the spindle microtubules.
microtubule cytoskeleton	The part of the cytoskeleton (the internal framework of a cell) composed of microtubules and associated proteins.
midbody	A thin cytoplasmic bridge formed between daughter cells at the end of cytokinesis. The midbody forms where the contractile ring constricts, and may persist for some time before finally breaking to complete cytokinesis.
mitotic spindle pole	Either of the ends of a mitotic spindle, a spindle that forms as part of mitosis, where spindle microtubules are organized; usually contains a microtubule organizing center and accessory molecules, spindle microtubules and astral microtubules.
nucleoplasm	That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
outer kinetochore	The region of a kinetochore most external to centromeric DNA; this outer region mediates kinetochore-microtubule interactions.
spindle	The array of microtubules and associated molecules that forms between opposite poles of a eukaryotic cell during mitosis or meiosis and serves to move the duplicated chromosomes apart.
spindle midzone	The area in the center of the spindle where the spindle microtubules from opposite poles overlap.
spindle pole	Either of the ends of a spindle, where spindle microtubules are organized; usually contains a microtubule organizing center and accessory molecules, spindle microtubules and astral microtubules.
synaptonemal complex	A proteinaceous scaffold found between homologous chromosomes during meiosis. It consists of 2 lateral elements and a central element, all running parallel to each other. Transverse filaments connect the lateral elements to the central element.

10 GO annotations of molecular function

Name	Definition
anaphase-promoting complex binding	Binding to an anaphase-promoting complex. A ubiquitin ligase complex that degrades mitotic cyclins and anaphase inhibitory protein, thereby triggering sister chromatid separation and exit from mitosis.
ATP binding	Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
identical protein binding	Binding to an identical protein or proteins.
magnesium ion binding	Binding to a magnesium (Mg) ion.
microtubule binding	Binding to a microtubule, a filament composed of tubulin monomers.
protein kinase activity	Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP.
protein kinase binding	Binding to a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate.
protein serine kinase activity	Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine kinase activity	Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
protein serine/threonine/tyrosine kinase activity	Catalysis of the reactions: ATP + a protein serine = ADP + protein serine phosphate; ATP + a protein threonine = ADP + protein threonine phosphate; and ATP + a protein tyrosine = ADP + protein tyrosine phosphate.

42 GO annotations of biological process

Name	Definition
centrosome cycle	The cell cycle process in which centrosome duplication and separation takes place. The centrosome cycle can operate with a considerable degree of independence from other processes of the cell cycle.
establishment of mitotic spindle orientation	A cell cycle process that sets the alignment of mitotic spindle relative to other cellular structures.
establishment of protein localization	The directed movement of a protein to a specific location.
female meiosis chromosome segregation	The cell cycle process in which genetic material, in the form of chromosomes, is organized and then physically separated and apportioned to two or more sets during the meiotic cell cycle in a female.
G2/M transition of mitotic cell cycle	The mitotic cell cycle transition by which a cell in G2 commits to M phase. The process begins when the kinase activity of M cyclin/CDK complex reaches a threshold high enough for the cell cycle to proceed. This is accomplished by activating a positive feedback loop that results in the accumulation of unphosphorylated and active M cyclin/CDK complex.
homologous chromosome segregation	The cell cycle process in which replicated homologous chromosomes are organized and then physically separated and apportioned to two sets during the first division of the meiotic cell cycle. Each replicated chromosome, composed of two sister chromatids, aligns at the cell equator, paired with its homologous partner; this pairing off, referred to as synapsis, permits genetic recombination. One homolog (both sister chromatids) of each morphologic type goes into each of the resulting chromosome sets.
microtubule bundle formation	A process that results in a parallel arrangement of microtubules.
mitotic cell cycle	Progression through the phases of the mitotic cell cycle, the most common eukaryotic cell cycle, which canonically comprises four successive phases called G1, S, G2, and M and includes replication of the genome and the subsequent segregation of chromosomes into daughter cells. In some variant cell cycles nuclear replication or nuclear division may not be followed by cell division, or G1 and G2 phases may be absent.
mitotic chromosome condensation	The cell cycle process in which chromatin structure is compacted prior to and during mitosis in eukaryotic cells.
mitotic cytokinesis	A cell cycle process that results in the division of the cytoplasm of a cell after mitosis, resulting in the separation of the original cell into two daughter cells.
mitotic G2 DNA damage checkpoint signaling	A mitotic cell cycle checkpoint that detects and negatively regulates progression through the G2/M transition of the cell cycle in response to DNA damage.
mitotic nuclear membrane disassembly	The mitotic cell cycle process in which the controlled partial or complete breakdown of the nuclear membranes during occurs during mitosis.
mitotic sister chromatid segregation	The cell cycle process in which replicated homologous chromosomes are organized and then physically separated and apportioned to two sets during the mitotic cell cycle. Each replicated chromosome, composed of two sister chromatids, aligns at the cell equator, paired with its homologous partner. One homolog of each morphologic type goes into each of the resulting chromosome sets.
mitotic spindle assembly checkpoint signaling	A signal transduction process that contributes to a mitotic cell cycle spindle assembly checkpoint, that delays the metaphase/anaphase transition of a mitotic nuclear division until the spindle is correctly assembled and chromosomes are attached to the spindle.
mitotic spindle organization	A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the microtubule spindle during a mitotic cell cycle.
negative regulation of apoptotic process	Any process that stops, prevents, or reduces the frequency, rate or extent of cell death by apoptotic process.
negative regulation of cyclin-dependent protein serine/threonine kinase activity	Any process that stops, prevents, or reduces the frequency, rate or extent of cyclin-dependent protein serine/threonine kinase activity.
negative regulation of transcription by RNA polymerase II	Any process that stops, prevents, or reduces the frequency, rate or extent of transcription mediated by RNA polymerase II.
nuclear membrane disassembly	The controlled breakdown of the nuclear membranes, for example during cellular division.
peptidyl-serine phosphorylation	The phosphorylation of peptidyl-serine to form peptidyl-O-phospho-L-serine.
positive regulation of peptidyl-threonine phosphorylation	Any process that increases the frequency, rate or extent of peptidyl-threonine phosphorylation. Peptidyl-threonine phosphorylation is the phosphorylation of peptidyl-threonine to form peptidyl-O-phospho-L-threonine.
positive regulation of proteasomal ubiquitin-dependent protein catabolic process	Any process that activates or increases the frequency, rate or extent of the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of ubiquitin, and mediated by the proteasome.
positive regulation of protein localization to nucleus	Any process that activates or increases the frequency, rate or extent of protein localization to nucleus.
positive regulation of proteolysis	Any process that activates or increases the frequency, rate or extent of the hydrolysis of a peptide bond or bonds within a protein.
positive regulation of ubiquitin protein ligase activity	Any process that activates or increases the frequency, rate or extent of ubiquitin protein ligase activity.
positive regulation of ubiquitin-protein transferase activity	Any process that activates, maintains or increases the rate of ubiquitin transferase activity.
protein destabilization	Any process that decreases the stability of a protein, making it more vulnerable to degradative processes or aggregation.
protein localization to chromatin	Any process in which a protein is transported to, or maintained at, a part of a chromosome that is organized into chromatin.
protein localization to nuclear envelope	A process in which a protein is transported to, or maintained at, a location within a nuclear envelope.
protein phosphorylation	The process of introducing a phosphate group on to a protein.
protein ubiquitination	The process in which one or more ubiquitin groups are added to a protein.
regulation of anaphase-promoting complex-dependent catabolic process	Any process that modulates the frequency, rate or extent of anaphase-promoting complex-dependent catabolic process.
regulation of cell cycle	Any process that modulates the rate or extent of progression through the cell cycle.
regulation of cytokinesis	Any process that modulates the frequency, rate or extent of the division of the cytoplasm of a cell and its separation into two daughter cells.
regulation of mitotic cell cycle	Any process that modulates the rate or extent of progress through the mitotic cell cycle.
regulation of mitotic cell cycle phase transition	Any process that modulates the frequency, rate or extent of mitotic cell cycle phase transition.
regulation of mitotic metaphase/anaphase transition	Any process that modulates the frequency, rate or extent of the cell cycle process in which a cell progresses from metaphase to anaphase during mitosis, triggered by the activation of the anaphase promoting complex by Cdc20/Sleepy homolog which results in the degradation of Securin.
regulation of mitotic spindle assembly	Any process that modulates the frequency, rate or extent of mitotic spindle assembly.
regulation of protein binding	Any process that modulates the frequency, rate or extent of protein binding.
regulation of protein localization to cell cortex	Any process that modulates the frequency, rate or extent of protein localization to cell cortex.
sister chromatid cohesion	The cell cycle process in which the sister chromatids of a replicated chromosome become tethered to each other.
synaptonemal complex disassembly	The controlled breakdown of a synaptonemal complex.

9 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q2TA25	PLK1	Serine/threonine-protein kinase PLK1	Bos taurus (Bovine)	SS
O97143	SAK	Serine/threonine-protein kinase PLK4	Drosophila melanogaster (Fruit fly)	EV
P52304	polo	Serine/threonine-protein kinase polo	Drosophila melanogaster (Fruit fly)	SS
Q07832	Plk1	Serine/threonine-protein kinase PLK1	Mus musculus (Mouse)	EV
Q62673	Plk1	Serine/threonine-protein kinase PLK1	Rattus norvegicus (Rat)	SS
Q20845	plk-3	Serine/threonine-protein kinase plk-3	Caenorhabditis elegans	PR
Q9N2L7	plk-2	Serine/threonine-protein kinase plk-2	Caenorhabditis elegans	SS
P34331	plk-1	Serine/threonine-protein kinase plk-1	Caenorhabditis elegans	SS
P62205	plk1	Serine/threonine-protein kinase PLK1	Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)	SS

10	20	30	40	50	60
MSAAVTAGKL	ARAPADPGKA	GVPGVAAPGA	PAAAPPAKEI	PEVLVDPRSR	RRYVRGRFLG
70	80	90	100	110	120
KGGFAKCFEI	SDADTKEVFA	GKIVPKSLLL	KPHQREKMSM	EISIHRSLAH	QHVVGFHGFF
130	140	150	160	170	180
EDNDFVFVVL	ELCRRRSLLE	LHKRRKALTE	PEARYYLRQI	VLGCQYLHRN	RVIHRDLKLG
190	200	210	220	230	240
NLFLNEDLEV	KIGDFGLATK	VEYDGERKKT	LCGTPNYIAP	EVLSKKGHSF	EVDVWSIGCI
250	260	270	280	290	300
MYTLLVGKPP	FETSCLKETY	LRIKKNEYSI	PKHINPVAAS	LIQKMLQTDP	TARPTINELL
310	320	330	340	350	360
NDEFFTSGYI	PARLPITCLT	IPPRFSIAPS	SLDPSNRKPL	TVLNKGLENP	LPERPREKEE
370	380	390	400	410	420
PVVRETGEVV	DCHLSDMLQQ	LHSVNASKPS	ERGLVRQEEA	EDPACIPIFW	VSKWVDYSDK
430	440	450	460	470	480
YGLGYQLCDN	SVGVLFNDST	RLILYNDGDS	LQYIERDGTE	SYLTVSSHPN	SLMKKITLLK
490	500	510	520	530	540
YFRNYMSEHL	LKAGANITPR	EGDELARLPY	LRTWFRTRSA	IILHLSNGSV	QINFFQDHTK
550	560	570	580	590	600
LILCPLMAAV	TYIDEKRDFR	TYRLSLLEEY	GCCKELASRL	RYARTMVDKL	LSSRSASNRL

KAS