P49071

SS
Gene name
MAPk-Ak2 (CG3086)
Protein name
MAP kinase-activated protein kinase 2
Names
MAPK-activated protein kinase 2 , MAPKAP kinase 2 , MAPKAPK-2 , EC 2.7.11.1
Species
Drosophila melanogaster (Fruit fly)
KEGG Pathway
dme:Dmel_CG3086
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

MAPKAPK2 is a stress-activated serine/threonine-protein kinase. MAPKAPK2, contains an autoinhibitory α-helix overlapping with a leucine-rich nuclear export signal (NES), and also lies close to the nuclear localization signal (NLS). The closed conformation of MAPKAPK2 prevents exportin binding to NES. Phosphorylation of MAPKAPK2 at threonine 317 induces a conformational change, thus unmasks and increases the activity of NES.

Autoinhibitory domains (AIDs)

288-308 (Autoinhibitory A-helix) SS

Target domain

0-281 (Protein kinase domain)

Relief mechanism

PTM

Assay

Accessory elements

162-184 (Activation loop from InterPro)

Target domain

20-281 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for P49071

Entry ID Method Resolution Chain Position Source
AF-P49071-F1 Predicted AlphaFoldDB

No variants for P49071

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P49071

No associated diseases with P49071

3 regional properties for P49071

Type Name Position InterPro Accession
domain Protein kinase domain 20 - 281 IPR000719
active_site Serine/threonine-protein kinase, active site 138 - 150 IPR008271
binding_site Protein kinase, ATP binding site 26 - 49 IPR017441

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

7 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
calcium-dependent protein serine/threonine kinase activity Calcium-dependent catalysis of the reactions
calmodulin binding Binding to calmodulin, a calcium-binding protein with many roles, both in the calcium-bound and calcium-free states.
calmodulin-dependent protein kinase activity Calmodulin-dependent catalysis of the reactions
mitogen-activated protein kinase binding Binding to a mitogen-activated protein kinase.
protein kinase activity Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction
protein serine kinase activity Catalysis of the reactions

5 GO annotations of biological process

Name Definition
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
positive regulation of cell size Any process that increases cell size.
protein phosphorylation The process of introducing a phosphate group on to a protein.
regulation of JNK cascade Any process that modulates the frequency, rate or extent of signal transduction mediated by the JNK cascade.
response to salt stress Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating an increase or decrease in the concentration of salt (particularly but not exclusively sodium and chloride ions) in the environment.

9 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q3SYZ2 MAPKAPK3 MAP kinase-activated protein kinase 3 Bos taurus (Bovine) SS
Q8IW41 MAPKAPK5 MAP kinase-activated protein kinase 5 Homo sapiens (Human) SS
Q16644 MAPKAPK3 MAP kinase-activated protein kinase 3 Homo sapiens (Human) SS
P49137 MAPKAPK2 MAP kinase-activated protein kinase 2 Homo sapiens (Human) EV
O54992 Mapkapk5 MAP kinase-activated protein kinase 5 Mus musculus (Mouse) EV
P49138 Mapkapk2 MAP kinase-activated protein kinase 2 Mus musculus (Mouse) EV
Q3UMW7 Mapkapk3 MAP kinase-activated protein kinase 3 Mus musculus (Mouse) SS
Q66H84 Mapkapk3 MAP kinase-activated protein kinase 3 Rattus norvegicus (Rat) SS
Q965G5 mak-2 MAP kinase-activated protein kinase mak-2 Caenorhabditis elegans SS
10 20 30 40 50 60
MLSLQNQRQP KTTPLTDDYV TSNTVLGYGI NGKVVQCTHR RTQQNYALKV LLDSERARRE
70 80 90 100 110 120
VDLHWRVSGC RYIVNIIDVY ENTFKDRKCL LVVMECMEGG ELFQRIQDKA DGAFTEREAA
130 140 150 160 170 180
QIMHEICAAV DYLHSRDIAH RDLKPENLLY TTTQPNATLK LTDFGFAKET FTSYTLQTPC
190 200 210 220 230 240
YTPYYVAPEV LGPEKYDKSC DIWSLGVVMY IIMCGFPPFY SNHGLAISPG MKNRIRTGQY
250 260 270 280 290 300
DFPDPEWTNV SQAAKDLIKG MLNVDPSKRL RIQDVISNKW IAQYNAVPQT PLCTGRMLKE
310 320 330 340 350
AEETWPEVQE EMTRSLATMR VDYDQMQIKA LDKSNNPLLT KRRKKIEEME LYMANATRN