P48608

EV
Gene name
dia
Protein name
Protein diaphanous
Names
Species
Drosophila melanogaster (Fruit fly)
KEGG Pathway
dme:Dmel_CG1768
EC number
Protein Class
PROTEIN DIAPHANOUS (PTHR45691)

Descriptions

Formins are conserved actin nucleators responsible for the assembly of diverse actin structures. The autoinhibition mechanism involves the intramolecular interaction between N-terminal DID domain and C-terminal DAD domain. Autoinhibition is relieved by GTP-bound Rho proteins, which bind to the DID and an adjacent GTPase binding (G) element, and consequently displace the DAD.

Autoinhibitory domains (AIDs)

114-369 (DID domain) EV

Target domain

59-241 (GBD domain)

Relief mechanism

Partner binding

Assay

Mutagenesis experiment, Structural analysis

991-1051 (DAD domain) EV

Target domain

59-241 (GBD domain)

Relief mechanism

Partner binding

Assay

Mutagenesis experiment, Structural analysis

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P48608

Entry ID Method Resolution Chain Position Source
AF-P48608-F1 Predicted AlphaFoldDB

No variants for P48608

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P48608

No associated diseases with P48608

6 regional properties for P48608

Type Name Position InterPro Accession
domain DRF autoregulatory 1027 - 1041 IPR010465
domain Formin, FH3 domain 246 - 444 IPR010472
domain Formin, GTPase-binding domain 59 - 241 IPR010473
domain Diaphanous autoregulatory (DAD) domain 1022 - 1054 IPR014767
domain Rho GTPase-binding/formin homology 3 (GBD/FH3) domain 59 - 431 IPR014768
domain Formin, FH2 domain 601 - 1044 IPR015425

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm, cytoskeleton
  • Cleavage furrow
  • Apical cell membrane
  • Localizes to the site where the metaphase furrow is anticipated to form, to the growing tip of cellularization furrows, and to contractile rings (PubMed:7821209)
  • Recruited to the apical cell membrane of epithelial cells in a Rho1 dependent manner, enhanced by phosphatidylinositol 4,5-bisphosphate (PtdIns[4,5]P(2)) enrichment established by sktl/skittles and Pten (PubMed:23853710)
PANTHER Family PTHR45691 PROTEIN DIAPHANOUS
PANTHER Subfamily PTHR45691:SF6 PROTEIN DIAPHANOUS
PANTHER Protein Class cytoskeletal protein
PANTHER Pathway Category No pathway information available

9 GO annotations of cellular component

Name Definition
apical part of cell The region of a polarized cell that forms a tip or is distal to a base. For example, in a polarized epithelial cell, the apical region has an exposed surface and lies opposite to the basal lamina that separates the epithelium from other tissue.
cell cortex The region of a cell that lies just beneath the plasma membrane and often, but not always, contains a network of actin filaments and associated proteins.
cleavage furrow The cleavage furrow is a plasma membrane invagination at the cell division site. The cleavage furrow begins as a shallow groove and eventually deepens to divide the cytoplasm.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytoskeleton A cellular structure that forms the internal framework of eukaryotic and prokaryotic cells. The cytoskeleton includes intermediate filaments, microfilaments, microtubules, the microtrabecular lattice, and other structures characterized by a polymeric filamentous nature and long-range order within the cell. The various elements of the cytoskeleton not only serve in the maintenance of cellular shape but also have roles in other cellular functions, including cellular movement, cell division, endocytosis, and movement of organelles.
M band The midline of aligned thick filaments in a sarcomere; location of specific proteins that link thick filaments. Depending on muscle type the M band consists of different numbers of M lines.
protein-containing complex A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together.
vesicle membrane The lipid bilayer surrounding any membrane-bounded vesicle in the cell.
Z disc Platelike region of a muscle sarcomere to which the plus ends of actin filaments are attached.

3 GO annotations of molecular function

Name Definition
actin binding Binding to monomeric or multimeric forms of actin, including actin filaments.
phosphatidylinositol-4,5-bisphosphate binding Binding to phosphatidylinositol-4,5-bisphosphate, a derivative of phosphatidylinositol in which the inositol ring is phosphorylated at the 4' and 5' positions.
small GTPase binding Binding to a small monomeric GTPase.

28 GO annotations of biological process

Name Definition
actin cytoskeleton reorganization A process that is carried out at the cellular level which results in dynamic structural changes to the arrangement of constituent parts of cytoskeletal structures comprising actin filaments and their associated proteins.
actin filament organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising actin filaments. Includes processes that control the spatial distribution of actin filaments, such as organizing filaments into meshworks, bundles, or other structures, as by cross-linking.
actin nucleation The initial step in the formation of an actin filament, in which actin monomers combine to form a new filament. Nucleation is slow relative to the subsequent addition of more monomers to extend the filament.
actomyosin contractile ring assembly The process of assembly of a ring composed of actin, myosin, and associated proteins that will function in cytokinesis.
apical constriction The actin-mediated process that results in the contraction of the apical end of a polarized columnar epithelial cell.
assembly of apicomedial cortex actomyosin A process which results in the assembly or arrangement of constituent parts apicomedial cortex actomyosin.
cellularization The separation of a multi-nucleate cell or syncytium into individual cells. An example of this is found in Drosophila melanogaster embryo development.
maintenance of protein location in cell Any process in which a protein is maintained in a specific location within, or in the membrane of, a cell, and is prevented from moving elsewhere.
meiosis I cytokinesis A cell cycle process that results in the division of the cytoplasm of a cell after meiosis I, resulting in the separation of the original cell into two daughter cells.
meiosis II cytokinesis A cell cycle process that results in the division of the cytoplasm of a cell after meiosis II, resulting in the separation of the original cell into two daughter cells.
melanotic encapsulation of foreign target Formation of a multilayered, melanized sheath of cells around a foreign invader.
mitotic cell cycle, embryonic The eukaryotic cell cycle in which a cell is duplicated without changing ploidy, occurring in the embryo.
mitotic cytokinesis A cell cycle process that results in the division of the cytoplasm of a cell after mitosis, resulting in the separation of the original cell into two daughter cells.
open tracheal system development The process whose specific outcome is the progression of an open tracheal system over time, from its formation to the mature structure. An open tracheal system is a respiratory system, a branched network of epithelial tubes that supplies oxygen to target tissues via spiracles. An example of this is found in Drosophila melanogaster.
plasma membrane tubulation A membrane tubulation process occurring in a plasma membrane.
pole cell formation Formation of a small group of cells (pole cells) at the posterior pole of the insect blastula. They are the first cells to cellularize after the arrival of nuclei at the end of the syncytial blastula stage and are the precursors to the insect germ cells.
positive regulation of actin filament polymerization Any process that activates or increases the frequency, rate or extent of actin polymerization.
positive regulation of wound healing Any process that increases the rate, frequency, or extent of the series of events that restore integrity to a damaged tissue, following an injury.
protein localization Any process in which a protein is transported to, or maintained in, a specific location.
protein secretion The controlled release of proteins from a cell.
pseudocleavage involved in syncytial blastoderm formation Formation of furrows in the cytoplasm between nuclei during cell cycles in embryos that contribute to the formation of the syncytial blastoderm. An example of this process is found in Drosophila melanogaster.
regulation of actomyosin structure organization Any process that modulates the frequency, rate or extent of the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures containing both actin and myosin or paramyosin.
regulation of embryonic cell shape Any process that modulates the surface configuration of an embryonic cell.
regulation of filopodium assembly Any process that modulates the frequency, rate or extent of the assembly of a filopodium, a thin, stiff protrusion extended by the leading edge of a motile cell such as a crawling fibroblast or amoeba, or an axonal growth cone.
salivary gland morphogenesis The process in which the anatomical structures of the salivary gland are generated and organized.
sarcomere organization The myofibril assembly process that results in the organization of muscle actomyosin into sarcomeres. The sarcomere is the repeating unit of a myofibril in a muscle cell, composed of an array of overlapping thick and thin filaments between two adjacent Z discs.
sensory perception of sound The series of events required for an organism to receive an auditory stimulus, convert it to a molecular signal, and recognize and characterize the signal. Sonic stimuli are detected in the form of vibrations and are processed to form a sound.
spindle assembly The aggregation, arrangement and bonding together of a set of components to form the spindle, the array of microtubules and associated molecules that serves to move duplicated chromosomes apart.

8 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q9NSV4 DIAPH3 Protein diaphanous homolog 3 Homo sapiens (Human) SS
O60610 DIAPH1 Protein diaphanous homolog 1 Homo sapiens (Human) SS
O60879 DIAPH2 Protein diaphanous homolog 2 Homo sapiens (Human) SS
O08808 Diaph1 Protein diaphanous homolog 1 Mus musculus (Mouse) EV
O70566 Diaph2 Protein diaphanous homolog 2 Mus musculus (Mouse) SS
Q9Z207 Diaph3 Protein diaphanous homolog 3 Mus musculus (Mouse) SS
F1M775 Diaph1 Protein diaphanous homolog 1 Rattus norvegicus (Rat) SS
F1LVW7 Diaph3 Protein diaphanous homolog 3 Rattus norvegicus (Rat) SS
10 20 30 40 50 60
MSRHEKTKST GGGLLDSLFG RPSKSKGGTI SSGTLAHGGR PVSADNYVVP GVEDFEQYIQ
70 80 90 100 110 120
QLSVAELDAK FLEIIEDMNI PKDKREPLLA KSKEERQKMI MWHLKGKNSL ERSANSRFEK
130 140 150 160 170 180
PIDYVEYLQN GEHSTHKVYQ CVESLRVALT SNPISWIKEF GVAGIGTIEK LLARSKNNAS
190 200 210 220 230 240
YEKIEFEAIR CLKAIMNNTW GLNVVLNPDQ HSVVLLLAQS LDPRKPQTMC EALKLLASFC
250 260 270 280 290 300
IVYERNGYEK VLRAITTIAA TSFKASERFR PIVDALFASD QQDPKRDLAC HSLIFINTLT
310 320 330 340 350 360
NTPTDLNFRL HLRCEIMRMG LYDRLDEFTK IVEASNNENL QQHFKIFNEI REDDFEEFVQ
370 380 390 400 410 420
RFDNVTFNMD DATDCFDVLK NLVTDTTSEP YFLSILQHLL YIRDDFYFRP AYYQLIEECI
430 440 450 460 470 480
SQIVFHKGYC DPNFENRNFN IDTSLLLDDI VEKAKAKESK RSEEYEKKIE QLESAKQEAE
490 500 510 520 530 540
AKAAHLEEKV KLMEANGVAA PSPNKLPKVN IPMPPPPPGG GGAPPPPPPP MPGRAGGGPP
550 560 570 580 590 600
PPPPPPMPGR AGGPPPPPPP PGMGGPPPPP MPGMMRPGGG PPPPPMMMGP MVPVLPHGLK
610 620 630 640 650 660
PKKKWDVKNP MKRANWKAIV PAKMSDKAFW VKCQEDKLAQ DDFLAELAVK FSSKPVKKEQ
670 680 690 700 710 720
KDAVDKPTTL TKKNVDLRVL DSKTAQNLAI MLGGSLKHLS YEQIKICLLR CDTDILSSNI
730 740 750 760 770 780
LQQLIQYLPP PEHLKRLQEI KAKGEPLPPI EQFAATIGEI KRLSPRLHNL NFKLTYADMV
790 800 810 820 830 840
QDIKPDIVAG TAACEEIRNS KKFSKILELI LLLGNYMNSG SKNEAAFGFE ISYLTKLSNT
850 860 870 880 890 900
KDADNKQTLL HYLADLVEKK FPDALNFYDD LSHVNKASRV NMDAIQKAMR QMNSAVKNLE
910 920 930 940 950 960
TDLQNNKVPQ CDDDKFSEVM GKFAEECRQQ VDVLGKMQLQ MEKLYKDLSE YYAFDPSKYT
970 980 990 1000 1010 1020
MEEFFADIKT FKDAFQAAHN DNVRVREELE KKRRLQEARE QSAREQQERQ QRKKAVVDMD
1030 1040 1050 1060 1070 1080
APQTQEGVMD SLLEALQTGS AFGQRNRQAR RQRPAGAERR AQLSRSRSRT RVTNGQLMTR
1090
EMILNEVLGS A