P47809

EV
Gene name
Map2k4 (Jnkk1, Mek4, Mkk4, Prkmk4, Sek1, Serk1, Skk1)
Protein name
Dual specificity mitogen-activated protein kinase kinase 4
Names
MAP kinase kinase 4, MAPKK 4, C-JUN N-terminal kinase kinase 1, JNK kinase 1, JNKK 1, JNK-activating kinase 1, MAPK/ERK kinase 4, MEK 4, SAPK/ERK kinase 1, SEK1
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:26398
EC number
2.7.12.2: Dual-specificity kinases (those acting on Ser/Thr and Tyr residues)
Protein Class

Descriptions

(Annotation based on sequence homology with P45985)
MAP2K4 encodes for Dual specificity mitogen-activated protein kinase kinase 4, belongs to MAP2Ks family, plays an important role in MAP kinase signal transduction pathway. The activation segment appears in a stable a-helical conformation. Phosphorylation of the activation segment destabilizes the autoinhibited state.

Autoinhibitory domains (AIDs)

Accessory elements

244-266 (Activation loop from InterPro)

Target domain

100-365 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for P47809

Entry ID Method Resolution Chain Position Source
AF-P47809-F1 Predicted AlphaFoldDB

No variants for P47809

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P47809

No associated diseases with P47809

3 regional properties for P47809

Type Name Position InterPro Accession
domain Protein kinase domain 199 - 527 IPR000719
active_site Serine/threonine-protein kinase, active site 320 - 332 IPR008271
binding_site Protein kinase, ATP binding site 205 - 228 IPR017441

Functions

Description
EC Number 2.7.12.2 Dual-specificity kinases (those acting on Ser/Thr and Tyr residues)
Subcellular Localization
  • Cytoplasm
  • Nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

5 GO annotations of cellular component

Name Definition
axon The long process of a neuron that conducts nerve impulses, usually away from the cell body to the terminals and varicosities, which are sites of storage and release of neurotransmitter.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
dendrite cytoplasm All of the contents of a dendrite, excluding the surrounding plasma membrane.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
perikaryon The portion of the cell soma (neuronal cell body) that excludes the nucleus.

7 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
JUN kinase kinase activity Catalysis of the phosphorylation of tyrosine and threonine residues in a c-Jun NH2-terminal kinase (JNK), a member of a subgroup of mitogen-activated protein kinases (MAPKs), which signal in response to cytokines and exposure to environmental stress. JUN kinase kinase (JNKK) is a dual-specificity protein kinase kinase and requires activation by a serine/threonine kinase JUN kinase kinase kinase.
MAP kinase kinase activity Catalysis of the concomitant phosphorylation of threonine (T) and tyrosine (Y) residues in a Thr-Glu-Tyr (TEY) thiolester sequence in a MAP kinase (MAPK) substrate.
mitogen-activated protein kinase kinase kinase binding Binding to a mitogen-activated protein kinase kinase kinase, a protein that can phosphorylate a MAP kinase kinase.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
protein tyrosine kinase activity Catalysis of the reaction: ATP + a protein tyrosine = ADP + protein tyrosine phosphate.

16 GO annotations of biological process

Name Definition
apoptotic process A programmed cell death process which begins when a cell receives an internal (e.g. DNA damage) or external signal (e.g. an extracellular death ligand), and proceeds through a series of biochemical events (signaling pathway phase) which trigger an execution phase. The execution phase is the last step of an apoptotic process, and is typically characterized by rounding-up of the cell, retraction of pseudopodes, reduction of cellular volume (pyknosis), chromatin condensation, nuclear fragmentation (karyorrhexis), plasma membrane blebbing and fragmentation of the cell into apoptotic bodies. When the execution phase is completed, the cell has died.
cell growth involved in cardiac muscle cell development The growth of a cardiac muscle cell, where growth contributes to the progression of the cell over time from its initial formation to its mature state.
cellular response to mechanical stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a mechanical stimulus.
JNK cascade An intracellular protein kinase cascade containing at least a JNK (a MAPK), a JNKK (a MAPKK) and a JUN3K (a MAP3K). The cascade can also contain an additional tier: the upstream MAP4K. The kinases in each tier phosphorylate and activate the kinases in the downstream tier to transmit a signal within a cell.
MAPK cascade An intracellular protein kinase cascade containing at least a MAPK, a MAPKK and a MAP3K. The cascade can also contain an additional tiers: the upstream MAP4K. The kinases in each tier phosphorylate and activate the kinase in the downstream tier to transmit a signal within a cell.
negative regulation of motor neuron apoptotic process Any process that stops, prevents or reduces the frequency, rate or extent of motor neuron apoptotic process.
positive regulation of apoptotic process Any process that activates or increases the frequency, rate or extent of cell death by apoptotic process.
positive regulation of DNA replication Any process that activates or increases the frequency, rate or extent of DNA replication.
positive regulation of JNK cascade Any process that activates or increases the frequency, rate or extent of signal transduction mediated by the JNK cascade.
positive regulation of JUN kinase activity Any process that activates or increases the frequency, rate or extent of JUN kinase activity.
positive regulation of neuron apoptotic process Any process that activates or increases the frequency, rate or extent of cell death of neurons by apoptotic process.
positive regulation of nitric-oxide synthase biosynthetic process Any process that activates or increases the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of a nitric oxide synthase enzyme.
positive regulation of protein phosphorylation Any process that activates or increases the frequency, rate or extent of addition of phosphate groups to amino acids within a protein.
positive regulation of smooth muscle cell apoptotic process Any process that activates or increases the frequency, rate, or extent of smooth muscle cell apoptotic process.
protein phosphorylation The process of introducing a phosphate group on to a protein.
response to wounding Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating damage to the organism.

14 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q5E9X2 MAP2K6 Dual specificity mitogen-activated protein kinase kinase 6 Bos taurus (Bovine) EV
P45985 MAP2K4 Dual specificity mitogen-activated protein kinase kinase 4 Homo sapiens (Human) EV
O14733 MAP2K7 Dual specificity mitogen-activated protein kinase kinase 7 Homo sapiens (Human) PR
P46734 MAP2K3 Dual specificity mitogen-activated protein kinase kinase 3 Homo sapiens (Human) SS
Q8CE90 Map2k7 Dual specificity mitogen-activated protein kinase kinase 7 Mus musculus (Mouse) PR
P70236 Map2k6 Dual specificity mitogen-activated protein kinase kinase 6 Mus musculus (Mouse) EV
O09110 Map2k3 Dual specificity mitogen-activated protein kinase kinase 3 Mus musculus (Mouse) PR
Q9WVS7 Map2k5 Dual specificity mitogen-activated protein kinase kinase 5 Mus musculus (Mouse) PR
Q63932 Map2k2 Dual specificity mitogen-activated protein kinase kinase 2 Mus musculus (Mouse) PR
P31938 Map2k1 Dual specificity mitogen-activated protein kinase kinase 1 Mus musculus (Mouse) PR
G5EDF7 sek-1 Dual specificity mitogen-activated protein kinase kinase sek-1 Caenorhabditis elegans PR
G5EDT6 jkk-1 Dual specificity mitogen-activated protein kinase kinase jkk-1 Caenorhabditis elegans PR
Q21307 mek-1 Dual specificity mitogen-activated protein kinase kinase mek-1 Caenorhabditis elegans PR
Q9DGE0 map2k6 Dual specificity mitogen-activated protein kinase kinase 6 Danio rerio (Zebrafish) (Brachydanio rerio) PR
10 20 30 40 50 60
MAAPSPSGGG GSGGGGGTPG PIGPPASGHP AVSSMQGKRK ALKLNFANPP VKSTARFTLN
70 80 90 100 110 120
PNTTGVQNPH IERLRTHSIE SSGKLKISPE QHWDFTAEDL KDLGEIGRGA YGSVNKMVHK
130 140 150 160 170 180
PSGQIMAVKR IRSTVDEKEQ KQLLMDLDVV MRSSDCPYIV QFYGALFREG DCWICMELMS
190 200 210 220 230 240
TSFDKFYKYV YSVLDDVIPE EILGKITLAT VKALNHLKEN LKIIHRDIKP SNILLDRSGN
250 260 270 280 290 300
IKLCDFGISG QLVDSIAKTR DAGCRPYMAP ERIDPSASRQ GYDVRSDVWS LGITLYELAT
310 320 330 340 350 360
GRFPYPKWNS VFDQLTQVVK GDPPQLSNSE EREFSPSFIN FVNLCLTKDE SKRPKYKELL
370 380 390
KHPFILMYEE RTVEVACYVC KILDQMPATP SSPMYVD