P46150
SS
Gene name |
Moe (EMR1, CG10701) |
Protein name |
Moesin/ezrin/radixin homolog 1 |
Names |
Ezrin-moesin-radixin 1 , Moesin , dMoesin , Protein D17 |
Species |
Drosophila melanogaster (Fruit fly) |
KEGG Pathway |
dme:Dmel_CG10701 |
EC number |
|
Protein Class |
|
Descriptions
MSN is a moesin protein and belongs to the ezrin-radixin-moesin (ERM) protein family, directly involved in the cytoskeleton-membrane crosslinking. Functional N-terminal FERM domain of ERM attaches to the membrane by binding specific membrane proteins while the last 34 residues of the C-terminal tail domain bind actin filaments. The autoinhibitory domain is positions at the C-terminal tail domain of ERM, where FERM domain of ERM is bound tightly via phosphotyrosine binding (PTB), pleckstrin homology (PH), and Enabled/VASP Homology 1 (EVH1) domains, thus masking the binding sites for other molecules. ERM is activated through phosphorylation at Thr558 weakening the FERM/tail binding and, unmasks the binding sites of membrane protein and actin filaments in the presence of phospholipids.
Autoinhibitory domains (AIDs)
Target domain |
6-296 (FERM domain) |
Relief mechanism |
PTM |
Assay |
|
Accessory elements
No accessory elements
References
- Yeon JH et al. (2016) "Systems-wide Identification of cis-Regulatory Elements in Proteins", Cell systems, 2, 89-100
- Pearson MA et al. (2000) "Structure of the ERM protein moesin reveals the FERM domain fold masked by an extended actin binding tail domain", Cell, 101, 259-70
- Austermann J et al. (2008) "Characterization of the Ca2+ -regulated ezrin-S100P interaction and its role in tumor cell migration", The Journal of biological chemistry, 283, 29331-40
- Liu J et al. (2014) "Conserved sequence repeats of IQGAP1 mediate binding to Ezrin", Journal of proteome research, 13, 1156-66
Autoinhibited structure
Activated structure
1 structures for P46150
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-P46150-F1 | Predicted | AlphaFoldDB |
No variants for P46150
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for P46150 | |||||
No associated diseases with P46150
6 regional properties for P46150
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | DEP domain | 216 - 291 | IPR000591 |
| domain | Cyclic nucleotide-binding domain | 43 - 162 | IPR000595-1 |
| domain | Cyclic nucleotide-binding domain | 356 - 475 | IPR000595-2 |
| domain | Ras-like guanine nucleotide exchange factor, N-terminal | 495 - 634 | IPR000651 |
| domain | Ras guanine-nucleotide exchange factors catalytic domain | 768 - 1010 | IPR001895 |
| conserved_site | Ras guanine-nucleotide exchange factor, conserved site | 921 - 950 | IPR019804 |
Functions
20 GO annotations of cellular component
| Name | Definition |
|---|---|
| adherens junction | A cell-cell junction composed of the epithelial cadherin-catenin complex. The epithelial cadherins, or E-cadherins, of each interacting cell extend through the plasma membrane into the extracellular space and bind to each other. The E-cadherins bind to catenins on the cytoplasmic side of the membrane, where the E-cadherin-catenin complex binds to cytoskeletal components and regulatory and signaling molecules. |
| apical part of cell | The region of a polarized cell that forms a tip or is distal to a base. For example, in a polarized epithelial cell, the apical region has an exposed surface and lies opposite to the basal lamina that separates the epithelium from other tissue. |
| apical plasma membrane | The region of the plasma membrane located at the apical end of the cell. |
| cell cortex | The region of a cell that lies just beneath the plasma membrane and often, but not always, contains a network of actin filaments and associated proteins. |
| cleavage furrow | The cleavage furrow is a plasma membrane invagination at the cell division site. The cleavage furrow begins as a shallow groove and eventually deepens to divide the cytoplasm. |
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| cytoplasmic side of apical plasma membrane | The side (leaflet) of the apical region of the plasma membrane that faces the cytoplasm. |
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| euchromatin | A dispersed and relatively uncompacted form of chromatin that is in a transcription-competent conformation. |
| extrinsic component of cytoplasmic side of plasma membrane | The component of a plasma membrane consisting of gene products and protein complexes that are loosely bound to its cytoplasmic surface, but not integrated into the hydrophobic region. |
| filopodium | Thin, stiff, actin-based protrusion extended by the leading edge of a motile cell such as a crawling fibroblast or amoeba, or an axonal or dendritic growth cone, or a dendritic shaft. |
| microvillus | Thin cylindrical membrane-covered projections on the surface of an animal cell containing a core bundle of actin filaments. Present in especially large numbers on the absorptive surface of intestinal cells. |
| mitotic spindle midzone | The area in the center of the anaphase spindle consisting of microtubules, microtubule bundling factors and kinesin motors where the spindle microtubules from opposite poles overlap in an antiparallel manner. |
| motile cilium | A cilium which may have a variable arrangement of axonemal microtubules and also contains molecular motors. It may beat with a whip-like pattern that promotes cell motility or transport of fluids and other cells across a cell surface, such as on epithelial cells that line the lumenal ducts of various tissues; or they may display a distinct twirling motion that directs fluid flow asymmetrically across the cellular surface to affect asymmetric body plan organization. Motile cilia can be found in single as well as multiple copies per cell. |
| nucleoplasm | That part of the nuclear content other than the chromosomes or the nucleolus. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
| polytene chromosome puff | A swelling at a site along the length of a polytene chromosome, thought to be the site of active transcription. |
| rhabdomere | The specialized microvilli-containing organelle on the apical surfaces of a photoreceptor cell containing the visual pigment rhodopsin and most of the proteins involved in phototransduction. |
| subapical complex | The most apical region of the lateral plasma membrane of an invertebrate epithelial cell. The subapical complex lies above the zonula adherens and the septate junction, and is comparable to the position of the tight junction of vertebrate cells. |
4 GO annotations of molecular function
| Name | Definition |
|---|---|
| actin binding | Binding to monomeric or multimeric forms of actin, including actin filaments. |
| cell adhesion molecule binding | Binding to a cell adhesion molecule. |
| microtubule binding | Binding to a microtubule, a filament composed of tubulin monomers. |
| phosphatidylinositol-4,5-bisphosphate binding | Binding to phosphatidylinositol-4,5-bisphosphate, a derivative of phosphatidylinositol in which the inositol ring is phosphorylated at the 4' and 5' positions. |
34 GO annotations of biological process
| Name | Definition |
|---|---|
| actin cytoskeleton organization | A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising actin filaments and their associated proteins. |
| actin filament-based process | Any cellular process that depends upon or alters the actin cytoskeleton, that part of the cytoskeleton comprising actin filaments and their associated proteins. |
| anterior/posterior pattern specification | The regionalization process in which specific areas of cell differentiation are determined along the anterior-posterior axis. The anterior-posterior axis is defined by a line that runs from the head or mouth of an organism to the tail or opposite end of the organism. |
| branching involved in open tracheal system development | The process in which the anatomical structures of branches in the open tracheal system are generated and organized. |
| cortical actin cytoskeleton organization | A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of actin-based cytoskeletal structures in the cell cortex, i.e. just beneath the plasma membrane. |
| cortical microtubule organization | A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of structures formed of microtubules and associated proteins in the cell cortex, i.e. just beneath the plasma membrane of a cell. |
| cytoskeleton organization | A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures. |
| determination of left/right symmetry | The establishment of an organism's body plan or part of an organism with respect to the left and right halves. The pattern can either be symmetric, such that the halves are mirror images, or asymmetric where the pattern deviates from this symmetry. |
| epithelial structure maintenance | A tissue homeostatic process required for the maintenance of epithelial structure. |
| establishment or maintenance of apical/basal cell polarity | Any cellular process that results in the specification, formation or maintenance polarization of a cell's architecture along its apical/basal axis so that the apical and basal regions of the cell have different membrane, extracellular matrix and sub-membrane cellular components. |
| establishment or maintenance of epithelial cell apical/basal polarity | Any cellular process that results in the specification, formation or maintenance of the apicobasal polarity of an epithelial cell. |
| establishment or maintenance of polarity of larval imaginal disc epithelium | Any cellular process that results in the specification, formation or maintenance of a polarized larval imaginal disc epithelium. |
| eye photoreceptor cell development | Development of a photoreceptor, a sensory cell in the eye that reacts to the presence of light. They usually contain a pigment that undergoes a chemical change when light is absorbed, thus stimulating a nerve. |
| lumen formation, open tracheal system | Creation of the central hole of a tube in an open tracheal system through which gases flow. |
| microtubule anchoring | Any process in which a microtubule is maintained in a specific location in a cell. |
| morphogenesis of an epithelium | The process in which the anatomical structures of epithelia are generated and organized. An epithelium consists of closely packed cells arranged in one or more layers, that covers the outer surfaces of the body or lines any internal cavity or tube. |
| oocyte anterior/posterior axis specification | Polarization of the oocyte along its anterior-posterior axis. An example of this is found in Drosophila melanogaster. |
| photoreceptor cell axon guidance | The chemotaxis process that directs the migration of a photoreceptor cell axon growth cone to its target in the optic lobe in response to a combination of attractive and repulsive cues. |
| pole plasm assembly | Establishment of the specialized cytoplasm found at the poles of the egg. An example of this is found in Drosophila melanogaster. |
| pole plasm protein localization | Any process in which a protein is transported to, or maintained in, the oocyte pole plasm. An example of this is found in Drosophila melanogaster. |
| positive regulation of cell population proliferation | Any process that activates or increases the rate or extent of cell proliferation. |
| positive regulation of early endosome to late endosome transport | Any process that activates or increases the frequency, rate or extent of early endosome to late endosome transport. |
| positive regulation of endocytosis | Any process that activates or increases the frequency, rate or extent of endocytosis. |
| positive regulation of mitotic spindle elongation | Any process that activates or increases the frequency, rate or extent of mitotic spindle elongation. |
| positive regulation of protein localization to early endosome | Any process that activates or increases the frequency, rate or extent of protein localization to early endosome. |
| protein localization | Any process in which a protein is transported to, or maintained in, a specific location. |
| protein localization to rhabdomere | A process in which a protein is transported to, or maintained in, a location within a rhabdomere. |
| regulation of actin cytoskeleton organization | Any process that modulates the frequency, rate or extent of the formation, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising actin filaments and their associated proteins. |
| regulation of cell shape | Any process that modulates the surface configuration of a cell. |
| regulation of membrane potential in photoreceptor cell | Hyperpolarization (vertebrates) or depolarization (invertebrates) of the photoreceptor cell membrane via closing/opening of cation specific channels as a result of signals generated by rhodopsin activation by a photon. |
| regulation of organelle assembly | Any process that modulates the frequency, rate or extent of organelle assembly. |
| rhabdomere development | The assembly and arrangement of a rhabdomere within a cell. The rhabdomere is the organelle on the apical surface of a photoreceptor cell that contains the visual pigments. |
| rhabdomere membrane biogenesis | A process that results in the biosynthesis of constituent macromolecules, assembly, and arrangement of constituent parts of a rhabdomere membrane. |
| RNA export from nucleus | The directed movement of RNA from the nucleus to the cytoplasm. |
21 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q2HJ49 | MSN | Moesin | Bos taurus (Bovine) | PR |
| Q32LP2 | RDX | Radixin | Bos taurus (Bovine) | SS |
| P31976 | EZR | Ezrin | Bos taurus (Bovine) | PR |
| Q9PU45 | RDX | Radixin | Gallus gallus (Chicken) | PR |
| Q24564 | Mer | Moesin/ezrin/radixin homolog 2 | Drosophila melanogaster (Fruit fly) | SS |
| Q3KP66 | INAVA | Innate immunity activator protein | Homo sapiens (Human) | PR |
| P35240 | NF2 | Merlin | Homo sapiens (Human) | EV |
| P35241 | RDX | Radixin | Homo sapiens (Human) | SS |
| P15311 | EZR | Ezrin | Homo sapiens (Human) | EV |
| P26038 | MSN | Moesin | Homo sapiens (Human) | EV |
| A2AD83 | Frmd7 | FERM domain-containing protein 7 | Mus musculus (Mouse) | PR |
| P46662 | Nf2 | Merlin | Mus musculus (Mouse) | SS |
| P26041 | Msn | Moesin | Mus musculus (Mouse) | SS |
| P26043 | Rdx | Radixin | Mus musculus (Mouse) | SS |
| P26040 | Ezr | Ezrin | Mus musculus (Mouse) | SS |
| P26042 | MSN | Moesin | Sus scrofa (Pig) | PR |
| P26044 | RDX | Radixin | Sus scrofa (Pig) | SS |
| Q63648 | Nf2 | Merlin | Rattus norvegicus (Rat) | SS |
| O35763 | Msn | Moesin | Rattus norvegicus (Rat) | SS |
| P31977 | Ezr | Ezrin | Rattus norvegicus (Rat) | SS |
| Q6Q413 | nf2b | NF2, moesin-ezrin-radixin-like | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSPKALNVRV | TTMDAELEFA | IQSTTTGKQL | FDQVVKTIGL | REVWFFGLQY | TDSKGDSTWI |
| 70 | 80 | 90 | 100 | 110 | 120 |
| KLYKKVMNQD | VKKENPLQFR | FRAKFYPEDV | AEELIQDITL | RLFYLQVKNA | ILTDEIYCPP |
| 130 | 140 | 150 | 160 | 170 | 180 |
| ETSVLLASYA | VQARHGDHNK | TTHTAGFLAN | DRLLPQRVID | QHKMSKDEWE | QSIMTWWQEH |
| 190 | 200 | 210 | 220 | 230 | 240 |
| RSMLREDAMM | EYLKIAQDLE | MYGVNYFEIR | NKKGTDLWLG | VDALGLNIYE | QDDRLTPKIG |
| 250 | 260 | 270 | 280 | 290 | 300 |
| FPWSEIRNIS | FSEKKFIIKP | IDKKAPDFMF | FAPRVRINKR | ILALCMGNHE | LYMRRRKPDT |
| 310 | 320 | 330 | 340 | 350 | 360 |
| IDVQQMKAQA | REEKNAKQQE | REKLQLALAA | RERAEKKQQE | YEDRLKQMQE | DMERSQRDLL |
| 370 | 380 | 390 | 400 | 410 | 420 |
| EAQDMIRRLE | EQLKQLQAAK | DELELRQKEL | QAMLQRLEEA | KNMEAVEKLK | LEEEIMAKQM |
| 430 | 440 | 450 | 460 | 470 | 480 |
| EVQRIQDEVN | AKDEETKRLQ | DEVEDARRKQ | VIAAEAAAAL | LAASTTPQHH | HVAEDENENE |
| 490 | 500 | 510 | 520 | 530 | 540 |
| EELTNGDAGG | DVSRDLDTDE | HIKDPIEDRR | TLAERNERLH | DQLKALKQDL | AQSRDETKET |
| 550 | 560 | 570 | |||
| ANDKIHRENV | RQGRDKYKTL | REIRKGNTKR | RVDQFENM |