AiPD: Autoinhibited Protein Database

P45985

Gene name	MAP2K4 (JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1)
Protein name	Dual specificity mitogen-activated protein kinase kinase 4
Names	MAP kinase kinase 4, MAPKK 4, JNK-activating kinase 1, MAPK/ERK kinase 4, MEK 4, SAPK/ERK kinase 1, SEK1, Stress-activated protein kinase kinase 1, SAPK kinase 1, SAPKK-1, SAPKK1, c-Jun N-terminal kinase kinase 1, JNKK
Species	Homo sapiens (Human)
KEGG Pathway	hsa:6416
EC number	2.7.12.2: Dual-specificity kinases (those acting on Ser/Thr and Tyr residues)
Protein Class	DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED (PTHR48013)

Descriptions

MAP2K4 encodes for Dual specificity mitogen-activated protein kinase kinase 4, belongs to MAP2Ks family, plays an important role in MAP kinase signal transduction pathway. The part of the activation (loop) segment is forming a helical structure (activation segment helix, ASH) in its autoinhibited state, which could be destabilized by the phosphorylation of the activation segment.

Autoinhibitory domains (AIDs)

Accessory elements

246-268 (Activation loop from InterPro)

Target domain	102-367 (Protein kinase domain)
Relief mechanism	PTM
Assay	Structure analysis, Mutagenesis experiment

References

Min X et al. (2009) "The structure of the MAP2K MEK6 reveals an autoinhibitory dimer", Structure (London, England : 1993), 17, 96-104

Shevchenko E et al. (2020) "The autoinhibited state of MKK4: Phosphorylation, putative dimerization and R134W mutant studied by molecular dynamics simulations", Computational and structural biotechnology journal, 18, 2687-2698

Autoinhibited structure

Activated structure

4 structures for P45985

Entry ID	Method	Resolution	Chain	Position	Source
3ALN	X-ray	230 A	A/B/C	80-399	PDB
3ALO	X-ray	260 A	A	80-399	PDB
3VUT	X-ray	350 A	A/B	80-399	PDB
AF-P45985-F1	Predicted				AlphaFoldDB

150 variants for P45985

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
CA398239990 rs1555550018 RCV000505661	180	L>V	Neuroblastoma [ClinVar]	Yes	ClinGen ClinVar Ensembl dbSNP
CA398214476 rs1417816691	4	P>L		No	ClinGen TOPMed
CA398214482 rs1161471824	5	S>I		No	ClinGen TOPMed gnomAD
rs1161471824 CA398214480	5	S>N		No	ClinGen TOPMed gnomAD
CA288025340 rs979899636	6	P>A		No	ClinGen TOPMed gnomAD
rs989028622 CA288025362	9	G>D		No	ClinGen TOPMed
CA288025359 rs959911955	9	G>R		No	ClinGen TOPMed gnomAD
CA288025358 rs959911955	9	G>S		No	ClinGen TOPMed gnomAD
CA398214514 rs1229178018	11	G>R		No	ClinGen TOPMed
rs749070408 CA8398836	15	G>S		No	ClinGen ExAC TOPMed gnomAD
CA288025374 VAR_062963 rs17855590	16	S>R		No	ClinGen UniProt TOPMed dbSNP gnomAD
CA398214563 rs1169380060	18	S>R		No	ClinGen TOPMed
rs976985059 CA288025382	19	G>D		No	ClinGen TOPMed
rs978668162 CA288025384	20	T>I		No	ClinGen TOPMed gnomAD
rs1289939331 CA398214576	21	P>A		No	ClinGen TOPMed gnomAD
CA398214582 rs527463657	22	G>C		No	ClinGen 1000Genomes TOPMed
CA288025392 rs527463657	22	G>S		No	ClinGen 1000Genomes TOPMed
rs1283698963 CA398214590	23	P>L		No	ClinGen TOPMed gnomAD
rs548788415 CA8398838	23	P>T		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs1049103069 CA288025394	26	S>Y		No	ClinGen TOPMed
rs1598004807 CA398214611	27	P>L		No	ClinGen Ensembl
CA288025399 rs910110079	28	A>S		No	ClinGen TOPMed
CA398214626 rs1283304214	30	G>S		No	ClinGen TOPMed
CA398214643 rs1326610085	32	P>Q		No	ClinGen gnomAD
CA398214647 rs1404280869	33	A>T		No	ClinGen TOPMed
CA288025413 rs561204979	33	A>V		No	ClinGen 1000Genomes gnomAD
CA288025420 rs940014479	34	V>F		No	ClinGen TOPMed gnomAD
CA398214651 rs940014479	34	V>I		No	ClinGen TOPMed gnomAD
rs1036999303 CA288025426	35	S>I		No	ClinGen TOPMed
rs1355620714 CA398214681	38	Q>*		No	ClinGen gnomAD
CA398214686 rs1214238261	38	Q>H		No	ClinGen gnomAD
CA8398850 rs760186886	41	R>H		No	ClinGen ExAC gnomAD
rs111801039 CA287997640	42	K>R		No	ClinGen Ensembl
rs752767497 CA8398852	45	K>M		No	ClinGen ExAC gnomAD
CA8398853 rs559800950	46	L>M		No	ClinGen 1000Genomes ExAC gnomAD
rs1348623447 CA398203507	47	N>S		No	ClinGen gnomAD
CA287997688 rs1010543298	50	N>S		No	ClinGen Ensembl
rs1447612385 CA398203569	50	N>Y		No	ClinGen TOPMed
rs1261150954 CA398203592	51	P>L		No	ClinGen TOPMed
rs754035296 CA8398855	52	P>A		No	ClinGen ExAC gnomAD
rs1358824984 COSM1686247 CA398203612	52	P>L	skin [Cosmic]	No	ClinGen cosmic curated TOPMed
rs754035296 CA398203604	52	P>S		No	ClinGen ExAC gnomAD
CA287997693 rs372872972	53	F>S		No	ClinGen ESP TOPMed gnomAD
rs1348785378 CA398203722	57	A>G		No	ClinGen gnomAD
rs757051936 CA8398856	57	A>T		No	ClinGen ExAC gnomAD
rs1213129161 CA398203759	59	F>L		No	ClinGen gnomAD
CA8398858 rs750208931	63	P>L		No	ClinGen ExAC gnomAD
CA8398857 rs778746854	63	P>S		No	ClinGen ExAC gnomAD
rs758381870 CA8398859	64	N>S		No	ClinGen ExAC gnomAD
rs780013239 CA8398860	66	T>A		No	ClinGen ExAC TOPMed gnomAD
rs201981451 CA287997743	68	V>A		No	ClinGen 1000Genomes TOPMed gnomAD
rs1394118458 CA398203862	69	Q>E		No	ClinGen gnomAD
rs1434938057 CA398203876	70	N>S		No	ClinGen gnomAD
CA8398886 rs377490722	76	L>V		No	ClinGen ESP ExAC gnomAD
CA398208127 rs1290976146	90	S>Y		No	ClinGen gnomAD
rs1466199105 CA398208154	94	H>Y		No	ClinGen TOPMed
CA288017479 rs773665341	105	L>F		No	ClinGen gnomAD
CA398208338 rs1474208027	108	I>F		No	ClinGen gnomAD
CA398208341 rs1215001531	108	I>T		No	ClinGen TOPMed
CA398208364 rs1164096095 COSM79380	110	R>*	ovary endometrium stomach [Cosmic]	No	ClinGen cosmic curated gnomAD
rs1418420168 CA398208374	111	G>*		No	ClinGen gnomAD
CA8398893 rs747647990	117	N>S		No	ClinGen ExAC TOPMed gnomAD
CA398208467 rs1290755523	118	K>N		No	ClinGen TOPMed gnomAD
rs769619151 CA8398894	120	V>I		No	ClinGen ExAC TOPMed gnomAD
rs769619151 CA398208496	120	V>L		No	ClinGen ExAC TOPMed gnomAD
CA288017491 rs77267737	130	V>G		No	ClinGen Ensembl
CA398239147 rs1415316526	136	T>I		No	ClinGen TOPMed gnomAD
COSM975559 CA8398968 rs775475276	137	V>M	endometrium [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
rs1444656785 CA398239163	138	D>N		No	ClinGen TOPMed
rs1371329502 CA398239180	139	E>K		No	ClinGen TOPMed
CA398239277 rs1307654543	146	L>V		No	ClinGen TOPMed
rs760515884 CA8398969	147	M>T		No	ClinGen ExAC TOPMed gnomAD
CA8398971 rs776387940	151	V>I		No	ClinGen ExAC gnomAD
CA8398972 rs761402309	153	M>V		No	ClinGen ExAC gnomAD
CA398239334 rs1208900089	154	R>Q		No	ClinGen gnomAD
CA398239332 rs1567657403 VAR_040819 COSM20411	154	R>W	large_intestine a colorectal adenocarcinoma sample; somatic mutation [Cosmic, UniProt]	No	ClinGen cosmic curated UniProt Ensembl dbSNP
rs1314005490 CA398239360	157	D>E		No	ClinGen TOPMed gnomAD
rs764582118 CA8398973	159	P>A		No	ClinGen ExAC gnomAD
rs1248970628 CA398239377	160	Y>C		No	ClinGen TOPMed gnomAD
rs749942107 CA8398974	160	Y>H		No	ClinGen ExAC gnomAD
CA398239412 rs1418890158	165	Y>N		No	ClinGen gnomAD
rs534883661 CA287984272	178	M>V		No	ClinGen Ensembl
rs1191386417 CA398240022	182	S>C		No	ClinGen gnomAD
rs766050183 CA8398995	185	F>L		No	ClinGen ExAC gnomAD
CA8398996 rs150905259	191	Y>C		No	ClinGen ESP ExAC TOPMed gnomAD
rs760844487 CA287984326	195	V>A		No	ClinGen Ensembl
rs764298113 CA8398998	195	V>I		No	ClinGen ExAC gnomAD
rs754217305 CA8398999	196	L>S		No	ClinGen ExAC gnomAD
CA398240251 rs1371909082	200	I>V		No	ClinGen TOPMed gnomAD
rs1040630581 CA287984336	203	E>D		No	ClinGen TOPMed
CA8399001 rs765746113	207	K>R		No	ClinGen ExAC gnomAD
rs1307489818 CA398240352	208	I>T		No	ClinGen gnomAD
CA398240362 rs1260446708	209	T>S		No	ClinGen gnomAD
CA398240370 rs1240723113	210	L>S		No	ClinGen gnomAD
rs1354928111 CA398241122	222	N>S		No	ClinGen TOPMed
CA287988423 rs748395840	230	I>T		No	ClinGen gnomAD
CA398241369 rs1597480176	231	K>N		No	ClinGen Ensembl
rs536223681 CA8399033	231	K>R		No	ClinGen 1000Genomes ExAC gnomAD
rs1025397785 CA287988437	242	N>S		No	ClinGen TOPMed
CA398241507 rs1160578914	243	I>T		No	ClinGen gnomAD
rs1361335563 CA398241605	255	V>L		No	ClinGen gnomAD
CA287988456 rs200798818	264	A>S		No	ClinGen 1000Genomes
rs76726054 CA287988466	266	C>W		No	ClinGen Ensembl
rs1455763623 CA398241772	277	P>A		No	ClinGen TOPMed
COSM21292 VAR_040822 CA8399062 rs753665559	279	A>T	large_intestine haematopoietic_and_lymphoid_tissue a colorectal adenocarcinoma sample; somatic mutation [Cosmic, UniProt]	No	ClinGen cosmic curated UniProt ExAC dbSNP gnomAD
rs1183392154 COSM1563407 CA398241789	279	A>V	large_intestine [Cosmic]	No	ClinGen cosmic curated Ensembl
CA8399063 rs537815982	286	V>I		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA398241845 rs1334746981	288	S>C		No	ClinGen gnomAD
CA8399090 rs144008624	301	A>G		No	ClinGen ESP ExAC
rs781002756 CA8399089	301	A>S		No	ClinGen ExAC gnomAD
rs1194360851 CA398241966	304	R>Q		No	ClinGen TOPMed gnomAD
rs755823478 CA8399091	309	K>R		No	ClinGen ExAC gnomAD
rs779390733 CA8399092	311	N>D		No	ClinGen ExAC gnomAD
rs1480785686 CA398242019	311	N>K		No	ClinGen TOPMed
rs1396947681 COSM98424 CA398242084	321	V>M	large_intestine [Cosmic]	No	ClinGen cosmic curated TOPMed gnomAD
CA288003875 COSM98425 rs267604739	326	P>L	large_intestine [Cosmic]	No	ClinGen cosmic curated Ensembl
rs1311024915 CA398242129	327	Q>H		No	ClinGen gnomAD
CA398242154 rs1597497681	331	S>Y		No	ClinGen Ensembl
CA398242159 rs1355543573	332	E>K		No	ClinGen gnomAD
rs908877597 CA288003883	335	E>Q		No	ClinGen TOPMed gnomAD
CA8399100 rs371746849	338	P>L		No	ClinGen ESP ExAC TOPMed gnomAD
CA398242205 rs371746849	338	P>R		No	ClinGen ESP ExAC TOPMed gnomAD
CA288003902 rs367710581	341	I>T		No	ClinGen ESP TOPMed
rs1239669542 CA398242223	341	I>V		No	ClinGen gnomAD
rs1355990355 CA398242234	342	N>K		No	ClinGen gnomAD
CA8399102 rs191244765	342	N>S		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs755523599 CA8399132 COSM975566	349	T>M	endometrium [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
rs1293062852 CA398242297	349	T>S		No	ClinGen gnomAD
rs1294064382 CA398242316	351	D>E		No	ClinGen gnomAD
rs1340390247 CA398242321	352	E>G		No	ClinGen gnomAD
CA288014390 rs1024493228	355	R>M		No	ClinGen TOPMed
CA8399135 rs769700685	359	K>T		No	ClinGen ExAC gnomAD
CA398242410 rs1567679461	363	K>R		No	ClinGen Ensembl
CA398242415 rs1323806098	364	H>Y		No	ClinGen TOPMed
CA398242443 rs1425777448	368	L>V		No	ClinGen gnomAD
CA398242461 rs1379788876	370	Y>C		No	ClinGen gnomAD
rs749364956 CA8399156	370	Y>N		No	ClinGen ExAC gnomAD
CA398242465 rs1168805084	371	E>K		No	ClinGen gnomAD
rs1300593472 CA398242483	373	R>C		No	ClinGen gnomAD
rs1372846289 CA398242484	373	R>H		No	ClinGen gnomAD
CA398242487 rs1052408848	374	A>S		No	ClinGen TOPMed
CA288015641 rs1052408848	374	A>T		No	ClinGen TOPMed
rs889363169 CA288015651	374	A>V		No	ClinGen TOPMed
rs367662793 CA8399158	375	V>I		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs771926980 CA8399160	378	A>S		No	ClinGen ExAC gnomAD
rs771926980 CA8399161 COSM975568	378	A>T	endometrium [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
CA398242567 rs1192323091	386	D>A		No	ClinGen TOPMed
CA398242633 rs1209244324	396	M>V		No	ClinGen gnomAD
rs538492504 CA8399165	397	Y>C		No	ClinGen ExAC TOPMed gnomAD
rs1177038824 CA398242655	399	D>N		No	ClinGen gnomAD

No associated diseases with P45985

3 regional properties for P45985

Type	Name	Position	InterPro Accession
domain	Protein kinase domain	102 - 367	IPR000719
active_site	Serine/threonine-protein kinase, active site	225 - 237	IPR008271
binding_site	Protein kinase, ATP binding site	108 - 131	IPR017441

Functions

		Description
EC Number	2.7.12.2	Dual-specificity kinases (those acting on Ser/Thr and Tyr residues)
Subcellular Localization	Cytoplasm Nucleus
PANTHER Family	PTHR48013	DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATED
PANTHER Subfamily	PTHR48013:SF35	DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 4
PANTHER Protein Class	non-receptor serine/threonine protein kinase protein modifying enzyme
PANTHER Pathway Category	Integrin signalling pathway Jnk FAS signaling pathway MKK4 p38 MAPK pathway MKK4 Huntington disease MAPKK4 Ras Pathway MKK4/7 Gonadotropin-releasing hormone receptor pathway MKK4/7 Huntington disease SEK-1 Apoptosis signaling pathway SEK1 Angiogenesis JNKK1 FGF signaling pathway MKK4,7 Integrin signalling pathway MEK EGF receptor signaling pathway MKK4,7 Oxidative stress response MKK4 Insulin/IGF pathway-mitogen activated protein kinase kinase/MAP kinase cascade MEK

2 GO annotations of cellular component

Name	Definition
cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

7 GO annotations of molecular function

Name	Definition
ATP binding	Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
JUN kinase kinase activity	Catalysis of the phosphorylation of tyrosine and threonine residues in a c-Jun NH2-terminal kinase (JNK), a member of a subgroup of mitogen-activated protein kinases (MAPKs), which signal in response to cytokines and exposure to environmental stress. JUN kinase kinase (JNKK) is a dual-specificity protein kinase kinase and requires activation by a serine/threonine kinase JUN kinase kinase kinase.
MAP kinase kinase activity	Catalysis of the concomitant phosphorylation of threonine (T) and tyrosine (Y) residues in a Thr-Glu-Tyr (TEY) thiolester sequence in a MAP kinase (MAPK) substrate.
protein kinase activity	Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP.
protein serine kinase activity	Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine kinase activity	Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
protein tyrosine kinase activity	Catalysis of the reaction: ATP + a protein tyrosine = ADP + protein tyrosine phosphate.

10 GO annotations of biological process

Name	Definition
apoptotic process	A programmed cell death process which begins when a cell receives an internal (e.g. DNA damage) or external signal (e.g. an extracellular death ligand), and proceeds through a series of biochemical events (signaling pathway phase) which trigger an execution phase. The execution phase is the last step of an apoptotic process, and is typically characterized by rounding-up of the cell, retraction of pseudopodes, reduction of cellular volume (pyknosis), chromatin condensation, nuclear fragmentation (karyorrhexis), plasma membrane blebbing and fragmentation of the cell into apoptotic bodies. When the execution phase is completed, the cell has died.
cellular response to mechanical stimulus	Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a mechanical stimulus.
cellular senescence	A cell aging process stimulated in response to cellular stress, whereby normal cells lose the ability to divide through irreversible cell cycle arrest.
Fc-epsilon receptor signaling pathway	The series of molecular signals initiated by the binding of the Fc portion of immunoglobulin E (IgE) to an Fc-epsilon receptor on the surface of a target cell, and ending with the regulation of a downstream cellular process, e.g. transcription. The Fc portion of an immunoglobulin is its C-terminal constant region.
JNK cascade	An intracellular protein kinase cascade containing at least a JNK (a MAPK), a JNKK (a MAPKK) and a JUN3K (a MAP3K). The cascade can also contain an additional tier: the upstream MAP4K. The kinases in each tier phosphorylate and activate the kinases in the downstream tier to transmit a signal within a cell.
MAPK cascade	An intracellular protein kinase cascade containing at least a MAPK, a MAPKK and a MAP3K. The cascade can also contain an additional tiers: the upstream MAP4K. The kinases in each tier phosphorylate and activate the kinase in the downstream tier to transmit a signal within a cell.
negative regulation of motor neuron apoptotic process	Any process that stops, prevents or reduces the frequency, rate or extent of motor neuron apoptotic process.
positive regulation of smooth muscle cell apoptotic process	Any process that activates or increases the frequency, rate, or extent of smooth muscle cell apoptotic process.
response to wounding	Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating damage to the organism.
signal transduction	The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.

15 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q5E9X2	MAP2K6	Dual specificity mitogen-activated protein kinase kinase 6	Bos taurus (Bovine)	EV
O14733	MAP2K7	Dual specificity mitogen-activated protein kinase kinase 7	Homo sapiens (Human)	PR
P52564	MAP2K6	Dual specificity mitogen-activated protein kinase kinase 6	Homo sapiens (Human)	EV
P46734	MAP2K3	Dual specificity mitogen-activated protein kinase kinase 3	Homo sapiens (Human)	SS
Q13163	MAP2K5	Dual specificity mitogen-activated protein kinase kinase 5	Homo sapiens (Human)	PR
P36507	MAP2K2	Dual specificity mitogen-activated protein kinase kinase 2	Homo sapiens (Human)	EV
Q02750	MAP2K1	Dual specificity mitogen-activated protein kinase kinase 1	Homo sapiens (Human)	EV
P47809	Map2k4	Dual specificity mitogen-activated protein kinase kinase 4	Mus musculus (Mouse)	EV
P70236	Map2k6	Dual specificity mitogen-activated protein kinase kinase 6	Mus musculus (Mouse)	EV
Q8CE90	Map2k7	Dual specificity mitogen-activated protein kinase kinase 7	Mus musculus (Mouse)	PR
O09110	Map2k3	Dual specificity mitogen-activated protein kinase kinase 3	Mus musculus (Mouse)	PR
G5EDF7	sek-1	Dual specificity mitogen-activated protein kinase kinase sek-1	Caenorhabditis elegans	PR
G5EDT6	jkk-1	Dual specificity mitogen-activated protein kinase kinase jkk-1	Caenorhabditis elegans	PR
Q21307	mek-1	Dual specificity mitogen-activated protein kinase kinase mek-1	Caenorhabditis elegans	PR
Q9DGE0	map2k6	Dual specificity mitogen-activated protein kinase kinase 6	Danio rerio (Zebrafish) (Brachydanio rerio)	PR

10	20	30	40	50	60
MAAPSPSGGG	GSGGGSGSGT	PGPVGSPAPG	HPAVSSMQGK	RKALKLNFAN	PPFKSTARFT
70	80	90	100	110	120
LNPNPTGVQN	PHIERLRTHS	IESSGKLKIS	PEQHWDFTAE	DLKDLGEIGR	GAYGSVNKMV
130	140	150	160	170	180
HKPSGQIMAV	KRIRSTVDEK	EQKQLLMDLD	VVMRSSDCPY	IVQFYGALFR	EGDCWICMEL
190	200	210	220	230	240
MSTSFDKFYK	YVYSVLDDVI	PEEILGKITL	ATVKALNHLK	ENLKIIHRDI	KPSNILLDRS
250	260	270	280	290	300
GNIKLCDFGI	SGQLVDSIAK	TRDAGCRPYM	APERIDPSAS	RQGYDVRSDV	WSLGITLYEL
310	320	330	340	350	360
ATGRFPYPKW	NSVFDQLTQV	VKGDPPQLSN	SEEREFSPSF	INFVNLCLTK	DESKRPKYKE
370	380	390
LLKHPFILMY	EERAVEVACY	VCKILDQMPA	TPSSPMYVD