P45818

PR
Gene name
ROK1 (YGL171W, G1651)
Protein name
ATP-dependent RNA helicase ROK1
Names
Rescuer of KEM1 protein 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YGL171W
EC number
3.6.4.13: Acting on ATP; involved in cellular and subcellular movement
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-143 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for P45818

Entry ID Method Resolution Chain Position Source
AF-P45818-F1 Predicted AlphaFoldDB

17 variants for P45818

Variant ID(s) Position Change Description Diseaes Association Provenance
s07-182460 22 K>T No SGRP
s07-182491 32 N>K No SGRP
s07-182663 90 V>M No SGRP
s07-182787 131 K>R No SGRP
s07-182828 145 P>T No SGRP
s07-182831 146 T>A No SGRP
s07-183044 217 Y>H No SGRP
s07-183134 247 D>N No SGRP
s07-183183 263 V>A No SGRP
s07-183258 288 K>R No SGRP
s07-183315 307 R>H No SGRP
s07-183473 360 I>V No SGRP
s07-183653 420 T>S No SGRP
s07-183924 510 R>K No SGRP
s07-184002 536 K>R No SGRP
s07-184073 560 K>E No SGRP
s07-184080 562 F>S No SGRP

No associated diseases with P45818

6 regional properties for P45818

Type Name Position InterPro Accession
domain Homeobox domain 227 - 294 IPR001356
domain ELK domain 205 - 226 IPR005539
domain KNOX1 69 - 113 IPR005540
domain KNOX2 123 - 174 IPR005541
domain Homeobox KN domain 247 - 286 IPR008422
conserved_site Homeobox, conserved site 265 - 288 IPR017970

Functions

Description
EC Number 3.6.4.13 Acting on ATP; involved in cellular and subcellular movement
Subcellular Localization
  • Nucleus, nucleolus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

4 GO annotations of cellular component

Name Definition
90S preribosome A large ribonucleoprotein complex considered to be the earliest preribosomal complex. In S. cerevisiae, it has a size of 90S and consists of the 35S pre-rRNA, early-associating ribosomal proteins most of which are part of the small ribosomal subunit, the U3 snoRNA and associated proteins.
nucleolus A small, dense body one or more of which are present in the nucleus of eukaryotic cells. It is rich in RNA and protein, is not bounded by a limiting membrane, and is not seen during mitosis. Its prime function is the transcription of the nucleolar DNA into 45S ribosomal-precursor RNA, the processing of this RNA into 5.8S, 18S, and 28S components of ribosomal RNA, and the association of these components with 5S RNA and proteins synthesized outside the nucleolus. This association results in the formation of ribonucleoprotein precursors; these pass into the cytoplasm and mature into the 40S and 60S subunits of the ribosome.
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
small-subunit processome A large ribonucleoprotein complex that is an early preribosomal complex. In S. cerevisiae, it has a size of 80S and consists of the 35S pre-rRNA, early-associating ribosomal proteins most of which are part of the small ribosomal subunit, the U3 snoRNA and associated proteins.

5 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient.
ATP-dependent activity, acting on RNA Catalysis of the reaction: ATP + H2O = ADP + phosphate; this reaction requires the presence of RNA, and it drives another reaction.
RNA binding Binding to an RNA molecule or a portion thereof.
RNA helicase activity Unwinding of an RNA helix, driven by ATP hydrolysis.

5 GO annotations of biological process

Name Definition
endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Endonucleolytic cleavage within the 5'-External Transcribed Spacer (ETS) of a tricistronic rRNA transcript that contains the Small Subunit (SSU) rRNA, the 5.8S rRNA, and the Large Subunit (LSU) rRNA in that order from 5' to 3' along the primary transcript. Endonucleolytic cleavage within the 5'-ETS of the pre-RNA is conserved as one of the early steps of rRNA processing in all eukaryotes, but the specific position of cleavage is variable.
endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Endonucleolytic cleavage between the SSU-rRNA and the 5.8S rRNA of an rRNA molecule originally produced as a tricistronic rRNA transcript that contained the Small SubUnit (SSU) rRNA, the 5.8S rRNA, and the Large SubUnit (LSU) rRNA, in that order, from 5' to 3' along the primary transcript.
endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Endonucleolytic cleavage between the 5'-External Transcribed Spacer (5'-ETS) and the 5' end of the SSU-rRNA of a tricistronic rRNA transcript that contains the Small Subunit (SSU) rRNA, the 5.8S rRNA, and the Large Subunit (LSU) rRNA in that order from 5' to 3' along the primary transcript, to produce the mature end of the SSU-rRNA.
maturation of SSU-rRNA Any process involved in the maturation of a precursor Small SubUnit (SSU) ribosomal RNA (rRNA) molecule into a mature SSU-rRNA molecule.
snoRNA localization Any process in which small nucleolar RNA is transported to, or maintained in, a specific location.

10 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P10081 TIF2 ATP-dependent RNA helicase eIF4A Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
P15424 MSS116 ATP-dependent RNA helicase MSS116, mitochondrial Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
P53166 MRH4 ATP-dependent RNA helicase MRH4, mitochondrial Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
Q03532 HAS1 ATP-dependent RNA helicase HAS1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
Q06218 DBP9 ATP-dependent RNA helicase DBP9 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
P20448 HCA4 ATP-dependent RNA helicase HCA4 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
P32892 DRS1 ATP-dependent RNA helicase DRS1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
P21372 PRP5 Pre-mRNA-processing ATP-dependent RNA helicase PRP5 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) EV
Q5K5B6 Os07g0647900 DEAD-box ATP-dependent RNA helicase 57 Oryza sativa subsp japonica (Rice) PR
Q84TG1 RH57 DEAD-box ATP-dependent RNA helicase 57 Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MDIFRVLTRG ASVKKESGPK AKAADYSVIN GNDENHKEDN NESQIVKELD FFRNKRIISK
70 80 90 100 110 120
VEDDREKTTE NDSPNKEEKS GNDDGLIKPV ITNTVEASAL RKSYKGNVSG IDIPLPIGSF
130 140 150 160 170 180
EDLISRFSFD KRLLNNLIEN GFTEPTPIQC ECIPVALNNR DVLACGPTGS GKTLAFLIPL
190 200 210 220 230 240
VQQIIDDKQT AGLKGLIISP TKELANQIFI ECFKLSYKIF LEKKRPLQVA LLSKSLGAKL
250 260 270 280 290 300
KNKVVSDKKY DIIISTPLRL IDVVKNEALD LSKVKHLIFD EADKLFDKTF VEQSDDILSA
310 320 330 340 350 360
CREPSLRKAM FSATIPSNVE EIAQSIMMDP VRVIIGHKEA ANTNIEQKLI FCGNEEGKLI
370 380 390 400 410 420
AIRQLVQEGE FKPPIIIFLE SITRAKALYH ELMYDRINVD VIHAERTALQ RDRIIERFKT
430 440 450 460 470 480
GELWCLICTD VLARGIDFKG VNLVINYDVP GSSQAYVHRI GRTGRGGRSG KAITFYTKQD
490 500 510 520 530 540
SVAIKPIINV MKQSGCEVSE WMDKMAKMTR KEKESIKNGK AHKERKQITT VPKMDKAKRR
550 560
RQQEMIAASK RRKNEELSKK HFSK