P41877
SS
Gene name |
isw-1 (F37A4.8) |
Protein name |
Chromatin-remodeling complex ATPase chain isw-1 |
Names |
EC 3.6.4.- , Nucleosome-remodeling factor subunit isw-1 |
Species |
Caenorhabditis elegans |
KEGG Pathway |
|
EC number |
3.6.4.-: Acting on ATP; involved in cellular and subcellular movement |
Protein Class |
|
Descriptions
SMARCA5 (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5, SNF2h) is a helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and a catalytic subunit of ISWI chromatin-remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair. <br>A highly conserved acidic patch formed by histones H2A and H2B of the nucleosome relieves the autoinhibition imposed by the AutoN and the NegC regions of the SMARCA5. In the autoinhibited state, AutoN and NegC hold the remodeler in an inactive state. The active state is promoted by AutoN and NegC binding near the acidic patch and by H4 tail binding to the ATPase domain. Thus, the acidic patch helps promote the translocation competent state of SMARCA5 by providing a binding site for NegC and AutoN.
Autoinhibitory domains (AIDs)
Target domain |
135-590 (Helicase domain) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
1 structures for P41877
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-P41877-F1 | Predicted | AlphaFoldDB |
No variants for P41877
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for P41877 | |||||
No associated diseases with P41877
10 regional properties for P41877
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | SNF2, N-terminal | 135 - 414 | IPR000330 |
| domain | SANT/Myb domain | 798 - 846 | IPR001005-1 |
| domain | SANT/Myb domain | 899 - 963 | IPR001005-2 |
| domain | Helicase, C-terminal domain-like | 436 - 590 | IPR001650 |
| domain | Helicase superfamily 1/2, ATP-binding domain | 128 - 320 | IPR014001 |
| domain | ISWI, HAND domain | 698 - 798 | IPR015194 |
| domain | SLIDE domain | 854 - 968 | IPR015195 |
| domain | SANT domain | 797 - 848 | IPR017884 |
| domain | Isw1/2, N-terminal | 129 - 349 | IPR044754 |
| domain | SNF2/RAD5-like, C-terminal helicase domain | 434 - 560 | IPR049730 |
Functions
| Description | ||
|---|---|---|
| EC Number | 3.6.4.- | Acting on ATP; involved in cellular and subcellular movement |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
2 GO annotations of cellular component
| Name | Definition |
|---|---|
| chromatin | The ordered and organized complex of DNA, protein, and sometimes RNA, that forms the chromosome. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
8 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| ATP hydrolysis activity | Catalysis of the reaction |
| ATP-dependent activity, acting on DNA | Catalytic activity that acts to modify DNA, driven by ATP hydrolysis. |
| ATP-dependent chromatin remodeler activity | An activity, driven by ATP hydrolysis, that modulates the contacts between histones and DNA, resulting in a change in chromosome architecture within the nucleosomal array, leading to chromatin remodeling. |
| DNA binding | Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid). |
| helicase activity | Catalysis of the reaction |
| histone binding | Binding to a histone, any of a group of water-soluble proteins found in association with the DNA of eukaryotic or archaeal chromosomes. They are involved in the condensation and coiling of chromosomes during cell division and have also been implicated in gene regulation and DNA replication. They may be chemically modified (methylated, acetlyated and others) to regulate gene transcription. |
| nucleosome binding | Binding to a nucleosome, a complex comprised of DNA wound around a multisubunit core and associated proteins, which forms the primary packing unit of DNA into higher order structures. |
3 GO annotations of biological process
| Name | Definition |
|---|---|
| nucleosome organization | A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of one or more nucleosomes. |
| positive regulation of transcription by RNA polymerase II | Any process that activates or increases the frequency, rate or extent of transcription from an RNA polymerase II promoter. |
| positive regulation of vulval development | Any process that activates or increases the frequency, rate or extent of development of the vulva. Vulval development is the process whose specific outcome is the progression of the egg-laying organ of female and hermaphrodite nematodes over time, from its formation to the mature structure. In nematodes, the vulva is formed from ventral epidermal cells during larval stages to give rise to a fully formed vulva in the adult. |
16 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P38144 | ISW1 | ISWI chromatin-remodeling complex ATPase ISW1 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| Q3B7N1 | CHD1L | Chromodomain-helicase-DNA-binding protein 1-like | Bos taurus (Bovine) | SS |
| Q24368 | Iswi | Chromatin-remodeling complex ATPase chain Iswi | Drosophila melanogaster (Fruit fly) | SS |
| Q86WJ1 | CHD1L | Chromodomain-helicase-DNA-binding protein 1-like | Homo sapiens (Human) | EV |
| Q9NRZ9 | HELLS | Lymphoid-specific helicase | Homo sapiens (Human) | PR |
| O60264 | SMARCA5 | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 | Homo sapiens (Human) | EV |
| P28370 | SMARCA1 | Probable global transcription activator SNF2L1 | Homo sapiens (Human) | SS |
| Q60848 | Hells | Lymphocyte-specific helicase | Mus musculus (Mouse) | PR |
| Q9CXF7 | Chd1l | Chromodomain-helicase-DNA-binding protein 1-like | Mus musculus (Mouse) | SS |
| Q91ZW3 | Smarca5 | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 | Mus musculus (Mouse) | SS |
| Q6PGB8 | Smarca1 | Probable global transcription activator SNF2L1 | Mus musculus (Mouse) | SS |
| P40201 | Chd1 | Chromodomain-helicase-DNA-binding protein 1 | Mus musculus (Mouse) | PR |
| Q7G8Y3 | Os01g0367900 | Probable chromatin-remodeling complex ATPase chain | Oryza sativa subsp japonica (Rice) | PR |
| Q22516 | chd-3 | Chromodomain-helicase-DNA-binding protein 3 homolog | Caenorhabditis elegans | PR |
| Q9XFH4 | DDM1 | ATP-dependent DNA helicase DDM1 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q8RWY3 | CHR11 | ISWI chromatin-remodeling complex ATPase CHR11 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSVHESSNEI | NDEPMDTDSN | IPESSGNDPS | MEVDDEPESS | DAADSFKRFE | RLLQKTENFS |
| 70 | 80 | 90 | 100 | 110 | 120 |
| HCLSSGDAKL | ATGAPVDTKK | RGRPSKKNGI | DGDHRHRKTE | QEEDEEMVAD | AIKSDDLVIF |
| 130 | 140 | 150 | 160 | 170 | 180 |
| DKSPFYIENG | EMRDYQVRGL | NWLASLQHNK | INGILADEMG | LGKTLQTISM | IGYMKHYKNK |
| 190 | 200 | 210 | 220 | 230 | 240 |
| ASPHLVIVPK | STLQNWANEF | KKWCPSINAV | VLIGDEAARN | QVLRDVILPQ | KFDVCCTTYE |
| 250 | 260 | 270 | 280 | 290 | 300 |
| MMLKVKTQLK | KLNWRYIIID | EAHRIKNEKS | KLSETVRELN | SENRLLITGT | PLQNNLHELW |
| 310 | 320 | 330 | 340 | 350 | 360 |
| ALLNFLLPDI | FTSSDDFDSW | FSNDAMSGNT | DLVQRLHKVL | QPFLLRRIKS | DVEKSLLPKK |
| 370 | 380 | 390 | 400 | 410 | 420 |
| EVKVYVGLSK | MQREWYTKVL | MKDIDIINGA | GKVEKARLMN | ILMHLRKCVN | HPYLFDGAEP |
| 430 | 440 | 450 | 460 | 470 | 480 |
| GPPFTTDQHL | VDNSGKMVVL | DKLLMKFKEQ | GSRVLIFSQF | SRMLDLLEDF | CWWRHYEYCR |
| 490 | 500 | 510 | 520 | 530 | 540 |
| LDGSTPHEDR | SNAIEAYNAP | DSKKFIFMLT | TRAGGLGINL | ATADVVIIYD | SDWNPQSDLQ |
| 550 | 560 | 570 | 580 | 590 | 600 |
| AMDRAHRIGQ | KKQVRVFRLI | TENTVDERII | EKAEAKLRLD | NIVIQQGRMS | EAQKTLGKGD |
| 610 | 620 | 630 | 640 | 650 | 660 |
| MISMIRHGAE | QVFAAKDSTI | SDDDIDTILE | KAEVKTAELN | EKMGKIDENN | LRNMTFEDNA |
| 670 | 680 | 690 | 700 | 710 | 720 |
| KFTVYQFEGE | NYKAKQADGM | GHFWIEPPKR | ERKANYQVDL | YYKEAMRAGN | PTEKQSKAPR |
| 730 | 740 | 750 | 760 | 770 | 780 |
| PKLPQVFDFQ | FYPRRLFELL | DKEIYHYRKT | IGYVAERPKD | VPPKEAEKRQ | AEEQKLINNA |
| 790 | 800 | 810 | 820 | 830 | 840 |
| RPLTDKEQEE | KAELLTQSVT | DWTKREFQQF | VRGNEKYGRE | DLESIAKEME | RPLEEIQSYA |
| 850 | 860 | 870 | 880 | 890 | 900 |
| KVFWERIEEL | QDSEKVLSQI | EKGEARIQRK | YAVKKALDAK | IAKYKAPFQQ | LRISYGTNKG |
| 910 | 920 | 930 | 940 | 950 | 960 |
| KTYTEEEDRF | LVCETHRLGH | DKENVFEELR | QSVRMAPQFR | FDWFLKSRTA | MELQRRCNTL |
| 970 | 980 | 990 | 1000 | ||
| ITLIEREMGE | VVESKPVIVT | AADKKKSVAK | DLSKSSGTPT | AKKVKATPK |