P41832
SS
Gene name |
BNI1 |
Protein name |
Protein BNI1 |
Names |
Pointed projection formation protein 3, Sensitive to high expression protein 5, Synthetic lethal 39 |
Species |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
KEGG Pathway |
sce:YNL271C |
EC number |
|
Protein Class |
|
Descriptions
(Annotation from UniProt)
The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments.
Autoinhibitory domains (AIDs)
Target domain |
174-696 (GBD/FH3 domain) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
No accessory elements
References
Autoinhibited structure
Activated structure
4 structures for P41832
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 1UX4 | X-ray | 330 A | A/B | 1352-1765 | PDB |
| 1UX5 | X-ray | 250 A | A | 1350-1760 | PDB |
| 1Y64 | X-ray | 305 A | B | 1327-1769 | PDB |
| AF-P41832-F1 | Predicted | AlphaFoldDB |
40 variants for P41832
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| s14-135053 | 111 | S>F | No | SGRP | |
| s14-134936 | 150 | S>L | No | SGRP | |
| s14-134871 | 172 | Y>H | No | SGRP | |
| s14-134682 | 235 | T>A | No | SGRP | |
| s14-134514 | 291 | V>I | No | SGRP | |
| s14-134508 | 293 | T>A | No | SGRP | |
| s14-134238 | 383 | A>T | No | SGRP | |
| s14-134188 | 399 | L>F | No | SGRP | |
| s14-133723 | 554 | M>I | No | SGRP | |
| s14-133596 | 597 | V>I | No | SGRP | |
| s14-133049 | 779 | A>V | No | SGRP | |
| s14-132930 | 819 | G>S | No | SGRP | |
| s14-132926 | 820 | T>S | No | SGRP | |
| s14-132647 | 913 | N>S | No | SGRP | |
| s14-132375 | 1004 | E>K | No | SGRP | |
| s14-132298 | 1029 | D>E | No | SGRP | |
| s14-132264 | 1041 | E>K | No | SGRP | |
| s14-132251 | 1045 | E>G | No | SGRP | |
| s14-132252 | 1045 | E>K | No | SGRP | |
| s14-131976 | 1137 | S>P | No | SGRP | |
| s14-131913 | 1158 | Y>H | No | SGRP | |
| s14-131841 | 1182 | A>T | No | SGRP | |
| s14-131820 | 1189 | D>Y | No | SGRP | |
| s14-131795 | 1197 | G>E | No | SGRP | |
| s14-131783 | 1201 | R>P | No | SGRP | |
| s14-131763 | 1208 | D>N | No | SGRP | |
| s14-131736 | 1217 | S>G | No | SGRP | |
| s14-131676 | 1237 | S>T | No | SGRP | |
| s14-131669 | 1239 | P>L | No | SGRP | |
| s14-131596 | 1263 | K>N | No | SGRP | |
| s14-131588 | 1266 | D>G | No | SGRP | |
| s14-131553 | 1278 | P>T | No | SGRP | |
| s14-131439 | 1316 | T>A | No | SGRP | |
| s14-131439 | 1316 | T>S | No | SGRP | |
| s14-131237 | 1383 | A>V | No | SGRP | |
| s14-131043 | 1448 | I>V | No | SGRP | |
| s14-130983 | 1468 | S>P | No | SGRP | |
| s14-130902 | 1495 | E>K | No | SGRP | |
| s14-130096 | 1763 | I>M | No | SGRP | |
| s14-129801 | 1862 | T>A | No | SGRP |
No associated diseases with P41832
4 regional properties for P41832
9 GO annotations of cellular component
| Name | Definition |
|---|---|
| actin filament | A filamentous structure formed of a two-stranded helical polymer of the protein actin and associated proteins. Actin filaments are a major component of the contractile apparatus of skeletal muscle and the microfilaments of the cytoskeleton of eukaryotic cells. The filaments, comprising polymerized globular actin molecules, appear as flexible structures with a diameter of 5-9 nm. They are organized into a variety of linear bundles, two-dimensional networks, and three dimensional gels. In the cytoskeleton they are most highly concentrated in the cortex of the cell just beneath the plasma membrane. |
| cell division site | The eventual plane of cell division (also known as cell cleavage or cytokinesis) in a dividing cell. In Eukaryotes, the cleavage apparatus, composed of septin structures and the actomyosin contractile ring, forms along this plane, and the mitotic, or meiotic, spindle is aligned perpendicular to the division plane. In bacteria, the cell division site is generally located at mid-cell and is the site at which the cytoskeletal structure, the Z-ring, assembles. |
| cellular bud neck | The constriction between the mother cell and daughter cell (bud) in an organism that reproduces by budding. |
| cellular bud tip | The end of a cellular bud distal to the site of attachment to the mother cell. |
| incipient cellular bud site | The portion of the budding yeast plasma membrane where a daughter cell will emerge. The yeast marks this spot with bud-site selection proteins before bud emergence occurs. Actin is polarized to this spot just prior to and during bud emergence. |
| mating projection tip | The apex of the mating projection in unicellular fungi exposed to mating pheromone; site of polarized growth. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
| polarisome | Protein complex that plays a role in determining cell polarity by directing the localized assembly of actin filaments at polarization sites; in Saccharomyces the polarisome includes Bni1p, Spa2p, Pea2p, and Bud6p. |
| prospore membrane | The prospore membrane is a double-membraned structure that extends from the cytoplasmic face of the spindle pole bodies to encompass the spindle pole bodies and the four nuclear lobes that are formed during meiosis. It helps isolate the meiotic nuclei from the cytoplasm during spore formation and serves as a foundation for the formation of the spore walls. An example of this component is found in Schizosaccharomyces pombe. |
4 GO annotations of molecular function
| Name | Definition |
|---|---|
| actin binding | Binding to monomeric or multimeric forms of actin, including actin filaments. |
| identical protein binding | Binding to an identical protein or proteins. |
| profilin binding | Binding to profilin, an actin-binding protein that forms a complex with G-actin and prevents it from polymerizing to form F-actin. |
| small GTPase binding | Binding to a small monomeric GTPase. |
9 GO annotations of biological process
| Name | Definition |
|---|---|
| actin filament bundle assembly | The assembly of actin filament bundles; actin filaments are on the same axis but may be oriented with the same or opposite polarities and may be packed with different levels of tightness. |
| actin nucleation | The initial step in the formation of an actin filament, in which actin monomers combine to form a new filament. Nucleation is slow relative to the subsequent addition of more monomers to extend the filament. |
| barbed-end actin filament capping | The binding of a protein or protein complex to the barbed (or plus) end of an actin filament, thus preventing the addition, exchange or removal of further actin subunits. |
| budding cell apical bud growth | Growth at the tip of a bud, in a cell that reproduces by budding. |
| establishment of mitotic spindle orientation | A cell cycle process that sets the alignment of mitotic spindle relative to other cellular structures. |
| formin-nucleated actin cable assembly | The aggregation, arrangement and bonding together of a set of components to form a formin-nucleated actin cable. A formin-nucleated actin cable is an actin filament bundle that consists of short filaments organized into bundles of uniform polarity, and is nucleated by formins. |
| mitotic actomyosin contractile ring assembly | Any actomyosin contractile ring assembly that is involved in mitotic cytokinesis. |
| positive regulation of actin cytoskeleton reorganization | Any process that activates or increases the frequency, rate or extent of actin cytoskeleton reorganization. |
| regulation of protein localization | Any process that modulates the frequency, rate or extent of any process in which a protein is transported to, or maintained in, a specific location. |
1 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P40450 | BNR1 | BNI1-related protein 1 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MLKNSGSKHS | NSKESHSNSS | SGIFQNLKRL | ANSNATNSNT | GSPTYASQQQ | HSPVGNEVST |
| 70 | 80 | 90 | 100 | 110 | 120 |
| SPASSSSFRK | LNAPSRSTST | EARPLNKKST | LNTQNLSQYM | NGKLSGDVPV | SSQHARSHSM |
| 130 | 140 | 150 | 160 | 170 | 180 |
| QSKYSYSKRN | SSQASNKLTR | QHTGQSHSAS | SLLSQGSLTN | LSKFTTPDGK | IYLEMPSDPY |
| 190 | 200 | 210 | 220 | 230 | 240 |
| EVEVLFEDIM | YKRNIFQSLS | EDKQEALMGY | SIEKKWLIVK | QDLQNELKKM | RANTTSSSTA |
| 250 | 260 | 270 | 280 | 290 | 300 |
| SRTSMASDHH | PILTANSSLS | SPKSVLMTSA | SSPTSTVYSN | SLNHSTTLSS | VGTSTSKGKK |
| 310 | 320 | 330 | 340 | 350 | 360 |
| LVSGSLKKQP | SLNNIYRGGA | ENNTSASTLP | GDRTNRPPIH | YVQRILADKL | TSDEMKDLWV |
| 370 | 380 | 390 | 400 | 410 | 420 |
| TLRTEQLDWV | DAFIDHQGHI | AMANVLMNSI | YKTAPRENLT | KELLEKENSF | FKCFRVLSML |
| 430 | 440 | 450 | 460 | 470 | 480 |
| SQGLYEFSTH | RLMTDTVAEG | LFSTKLATRK | MATEIFVCML | EKKNKSRFEA | VLTSLDKKFR |
| 490 | 500 | 510 | 520 | 530 | 540 |
| IGQNLHMIQN | FKKMPQYFSH | LTLESHLKII | QAWLFAVEQT | LDGRGKMGSL | VGASDEFKNG |
| 550 | 560 | 570 | 580 | 590 | 600 |
| GGENAILEYC | QWTMVFINHL | CSCSDNINQR | MLLRTKLENC | GILRIMNKIK | LLDYDKVIDQ |
| 610 | 620 | 630 | 640 | 650 | 660 |
| IELYDNNKLD | DFNVKLEANN | KAFNVDLHDP | LSLLKNLWDI | CKGTENEKLL | VSLVQHLFLS |
| 670 | 680 | 690 | 700 | 710 | 720 |
| SSKLIEENQN | SSKLTKQLKL | MDSLVTNVSV | ASTSDEETNM | NMAIQRLYDA | MQTDEVARRA |
| 730 | 740 | 750 | 760 | 770 | 780 |
| ILESRALTKK | LEEIQAERDS | LSEKLSKAEH | GLVGQLEDEL | HERDRILAKN | QRVMQQLEAE |
| 790 | 800 | 810 | 820 | 830 | 840 |
| LEELKKKHLL | EKHQQEVELR | KMLTILNSRP | EESFNKNEGT | RGMNSSLNSS | EKANIQKVLQ |
| 850 | 860 | 870 | 880 | 890 | 900 |
| DGLSRAKKDY | KDDSKKFGMT | LQPNKRLKML | RMQMENIENE | ARQLEMTNFA | EFEKDRLEPP |
| 910 | 920 | 930 | 940 | 950 | 960 |
| IHIKKPKVKK | MKNKDRKPLV | KPQEADVNKL | NDLRRALAEI | QMESNDISKF | NVEERVNELF |
| 970 | 980 | 990 | 1000 | 1010 | 1020 |
| NEKKSLALKR | LKELETKYKG | FGIDFNVDEI | MDSPKKNTGD | VETEEDANYA | SLDPKTYQKK |
| 1030 | 1040 | 1050 | 1060 | 1070 | 1080 |
| LDEINRITDQ | LLDIQTQTEH | EIQVEEDGES | DLSSSSSDDE | SEEIYQDASP | TQELRSEHSE |
| 1090 | 1100 | 1110 | 1120 | 1130 | 1140 |
| LSSGSGPGSF | LDALSQKYGT | GQNVTASAAF | GENNNGSGIG | PLHSKVEKTF | MNRLRKSTVS |
| 1150 | 1160 | 1170 | 1180 | 1190 | 1200 |
| SAPYLEELTQ | KVNKVEPYEQ | NEDEGLDKKS | LPENSTASAA | SAFDKAEKDM | RQHVENGKQG |
| 1210 | 1220 | 1230 | 1240 | 1250 | 1260 |
| RVVNHEEDKT | ADFSAVSKLN | NTDGAEDLST | QSSVLSSQPP | PPPPPPPPVP | AKLFGESLEK |
| 1270 | 1280 | 1290 | 1300 | 1310 | 1320 |
| EKKSEDDTVK | QETTGDSPAP | PPPPPPPPPP | PMALFGKPKG | ETPPPPPLPS | VLSSSTDGVI |
| 1330 | 1340 | 1350 | 1360 | 1370 | 1380 |
| PPAPPMMPAS | QIKSAVTSPL | LPQSPSLFEK | YPRPHKKLKQ | LHWEKLDCTD | NSIWGTGKAE |
| 1390 | 1400 | 1410 | 1420 | 1430 | 1440 |
| KFADDLYEKG | VLADLEKAFA | AREIKSLASK | RKEDLQKITF | LSRDISQQFG | INLHMYSSLS |
| 1450 | 1460 | 1470 | 1480 | 1490 | 1500 |
| VADLVKKILN | CDRDFLQTPS | VVEFLSKSEI | IEVSVNLARN | YAPYSTDWEG | VRNLEDAKPP |
| 1510 | 1520 | 1530 | 1540 | 1550 | 1560 |
| EKDPNDLQRA | DQIYLQLMVN | LESYWGSRMR | ALTVVTSYER | EYNELLAKLR | KVDKAVSALQ |
| 1570 | 1580 | 1590 | 1600 | 1610 | 1620 |
| ESDNLRNVFN | VILAVGNFMN | DTSKQAQGFK | LSTLQRLTFI | KDTTNSMTFL | NYVEKIVRLN |
| 1630 | 1640 | 1650 | 1660 | 1670 | 1680 |
| YPSFNDFLSE | LEPVLDVVKV | SIEQLVNDCK | DFSQSIVNVE | RSVEIGNLSD | SSKFHPLDKV |
| 1690 | 1700 | 1710 | 1720 | 1730 | 1740 |
| LIKTLPVLPE | ARKKGDLLED | EVKLTIMEFE | SLMHTYGEDS | GDKFAKISFF | KKFADFINEY |
| 1750 | 1760 | 1770 | 1780 | 1790 | 1800 |
| KKAQAQNLAA | EEEERLYIKH | KKIVEEQQKR | AQEKEKQKEN | SNSPSSEGNE | EDEAEDRRAV |
| 1810 | 1820 | 1830 | 1840 | 1850 | 1860 |
| MDKLLEQLKN | AGPAKSDPSS | ARKRALVRKK | YLSEKDNAPQ | LLNDLDTEEG | SILYSPEAMD |
| 1870 | 1880 | 1890 | 1900 | 1910 | 1920 |
| PTADTVIHAE | SPTPLATRGV | MNTSEDLPSP | SKTSALEDQE | EISDRARMLL | KELRGSDTPV |
| 1930 | 1940 | 1950 | |||
| KQNSILDEHL | EKLRARKERS | IGEASTGNRL | SFK |