P40994
SS
Gene name |
ARF3 (YOR094W, YOR3172W) |
Protein name |
ADP-ribosylation factor 3 |
Names |
|
Species |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
KEGG Pathway |
sce:YOR094W |
EC number |
|
Protein Class |
|
Descriptions
Small GTPases couple their GDP/GTP structural cycle to cytosol/membrane alternation to function as versatile molecular switches in the cell. Membrane localization of their active, GTP-bound form is pivotal to their ability to propagate information, and this requires their post-translational modification by lipids.<br>Arf GTPases are modified by a myristate attached to their N-terminus, which is shielded by intramolecular interactions in their inactive state. The myristoylated N-terminus of Arf is autoinhibitory in solution and is displaced by membranes, priming Arf GTPases for activation by their GEFs. Replacement of the N-terminal myristate by a 6xHis-tag preserves autoinhibition, representing that membranes unlock the N-terminal region to facilitate subsequent activation.
Autoinhibitory domains (AIDs)
Target domain |
20-142 (Small GTP-binding protein domain) |
Relief mechanism |
Ligand binding |
Assay |
|
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
1 structures for P40994
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-P40994-F1 | Predicted | AlphaFoldDB |
3 variants for P40994
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| s15-502827 | 11 | K>R | No | SGRP | |
| s15-502878 | 28 | A>V | No | SGRP | |
| s15-503294 | 167 | L>F | No | SGRP |
No associated diseases with P40994
No regional properties for P40994
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| No domain, repeats, and functional sites for P40994 | |||
6 GO annotations of cellular component
| Name | Definition |
|---|---|
| cellular bud neck | The constriction between the mother cell and daughter cell (bud) in an organism that reproduces by budding. |
| cellular bud tip | The end of a cellular bud distal to the site of attachment to the mother cell. |
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| endocytic vesicle | A membrane-bounded intracellular vesicle formed by invagination of the plasma membrane around an extracellular substance. Endocytic vesicles fuse with early endosomes to deliver the cargo for further sorting. |
| Golgi apparatus | A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways. |
| recycling endosome membrane | The lipid bilayer surrounding a recycling endosome. |
4 GO annotations of molecular function
| Name | Definition |
|---|---|
| enzyme activator activity | Binds to and increases the activity of an enzyme. |
| GTP binding | Binding to GTP, guanosine triphosphate. |
| GTPase activity | Catalysis of the reaction |
| mRNA binding | Binding to messenger RNA (mRNA), an intermediate molecule between DNA and protein. mRNA includes UTR and coding sequences, but does not contain introns. |
10 GO annotations of biological process
| Name | Definition |
|---|---|
| actin cortical patch localization | Any process in which actin cortical patches are transported to, or maintained in, a specific location. An actin cortical patch is a discrete actin-containing structure found just beneath the plasma membrane in fungal cells. |
| actin filament bundle assembly | The assembly of actin filament bundles; actin filaments are on the same axis but may be oriented with the same or opposite polarities and may be packed with different levels of tightness. |
| cellular bud site selection | The specification of the site where a daughter cell will form, in organisms that reproduce by budding. An example of this process is found in Saccharomyces cerevisiae. |
| endocytosis | A vesicle-mediated transport process in which cells take up external materials or membrane constituents by the invagination of a part of the plasma membrane to form a new membrane-bounded vesicle. |
| intracellular protein transport | The directed movement of proteins in a cell, including the movement of proteins between specific compartments or structures within a cell, such as organelles of a eukaryotic cell. |
| invasive filamentous growth | The growth of colonies in filamentous chains of cells into a substrate. |
| positive regulation of phosphatidylinositol biosynthetic process | Any process that increases the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of phosphatidylinositol. |
| protein localization to endosome | A process in which a protein is transported to, or maintained in, a location within an endosome. |
| regulation of dendritic spine development | Any process that modulates the rate, frequency, or extent of dendritic spine development, the process whose specific outcome is the progression of the dendritic spine over time, from its formation to the mature structure. |
| vesicle-mediated transport | A cellular transport process in which transported substances are moved in membrane-bounded vesicles; transported substances are enclosed in the vesicle lumen or located in the vesicle membrane. The process begins with a step that directs a substance to the forming vesicle, and includes vesicle budding and coating. Vesicles are then targeted to, and fuse with, an acceptor membrane. |
6 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P26990 | ARF6 | ADP-ribosylation factor 6 | Gallus gallus (Chicken) | SS |
| P40946 | Arf6 | ADP-ribosylation factor 6 | Drosophila melanogaster (Fruit fly) | SS |
| P62330 | ARF6 | ADP-ribosylation factor 6 | Homo sapiens (Human) | EV |
| P62331 | Arf6 | ADP-ribosylation factor 6 | Mus musculus (Mouse) | SS |
| Q007T5 | ARF6 | ADP-ribosylation factor 6 | Sus scrofa (Pig) | SS |
| P62332 | Arf6 | ADP-ribosylation factor 6 | Rattus norvegicus (Rat) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MGNSISKVLG | KLFGSKEMKI | LMLGLDKAGK | TTILYKLKLN | KIKTSTPTVG | FNVETVTYKN |
| 70 | 80 | 90 | 100 | 110 | 120 |
| VKFNMWDVGG | QQRLRPLWRH | YFPATTALIF | VIDSSARNRM | EEAKEELYSI | IGEKEMENVV |
| 130 | 140 | 150 | 160 | 170 | 180 |
| LLVWANKQDL | KDAMKPQEVS | DFLELEKNLK | NQPWCVIGSN | ALSGQGLVEG | LSWISNNTNV |
| PKK |