P40484
SS
Gene name |
MOB1 (YIL106W) |
Protein name |
DBF2 kinase activator protein MOB1 |
Names |
MPS1 binder 1 , Maintenance of ploidy protein MOB1 |
Species |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
KEGG Pathway |
sce:YIL106W |
EC number |
|
Protein Class |
|
Descriptions
MOB1A is a multifunctional protein involved in key signaling pathways like Hippo and NDR pathway signaling in metazoans and the Mitotic Exit Network in yeast. It regulates protein interactions by binding to upstream kinases MST1 and MST2 and downstream AGC group kinases LATS1, LATS2, NDR1, and NDR2. The autoinhibition of MOB1A occurs when its nonphosphorylated N-terminal extension binds to the core domain, blocking the kinase-binding site. This inhibitory interaction is relieved upon phosphorylation at specific sites (T12 and T35), which induces a conformational change that exposes the kinase-binding surface. Phosphorylation of MOB1A at T12 and T35 is crucial for regulating its interactions with both upstream and downstream kinases. The phosphorylation status dictates the binding affinity of MOB1A, enabling or inhibiting interactions with other proteins. The phosphorylation-dependent conformational changes in MOB1A are essential for the proper functioning of the Hippo signaling pathway, which is critical for cell proliferation and tissue growth regulation.
Autoinhibitory domains (AIDs)
Target domain |
152-314 (MOB1A core domain) |
Relief mechanism |
PTM |
Assay |
|
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
0 structures for P40484
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| No available structures | |||||
No variants for P40484
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for P40484 | |||||
No associated diseases with P40484
No regional properties for P40484
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| No domain, repeats, and functional sites for P40484 | |||
Functions
9 GO annotations of cellular component
| Name | Definition |
|---|---|
| cellular bud neck | The constriction between the mother cell and daughter cell (bud) in an organism that reproduces by budding. |
| chromosome, centromeric region | The region of a chromosome that includes the centromeric DNA and associated proteins. In monocentric chromosomes, this region corresponds to a single area of the chromosome, whereas in holocentric chromosomes, it is evenly distributed along the chromosome. |
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| mitotic spindle pole body | The microtubule organizing center that forms as part of the mitotic cell cycle; functionally homologous to the animal cell centrosome. |
| nuclear periphery | The portion of the nuclear lumen proximal to the inner nuclear membrane. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| serine/threonine protein kinase complex | A protein complex which is capable of protein serine/threonine kinase activity. |
| Sid2-Mob1 complex | A protein complex that contains a protein kinase (Sid2 in S. pombe) and its regulatory subunit (Mob1). The Sid2p-Mob1p kinase complex is a component of the septation initiation network in fission yeast (called the mitotic exit network in S. cerevisiae) and is required for cytokinesis. The analogous complex in S. cerevisiae is called Dbf2p-Mob1p complex. |
| spindle pole body | The microtubule organizing center in fungi; functionally homologous to the animal cell centrosome. |
3 GO annotations of molecular function
| Name | Definition |
|---|---|
| kinase regulator activity | Modulates the activity of a kinase, an enzyme which catalyzes of the transfer of a phosphate group, usually from ATP, to a substrate molecule. |
| metal ion binding | Binding to a metal ion. |
| protein kinase activator activity | Binds to and increases the activity of a protein kinase, an enzyme which phosphorylates a protein. |
8 GO annotations of biological process
| Name | Definition |
|---|---|
| cell division | The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells. |
| exit from mitosis | The cell cycle transition where a cell leaves M phase and enters a new G1 phase. M phase is the part of the mitotic cell cycle during which mitosis and cytokinesis take place. |
| hippo signaling | The series of molecular signals mediated by the serine/threonine kinase Hippo or one of its orthologs. In Drosophila, Hippo in complex with the scaffold protein Salvador (Sav), phosphorylates and activates Warts (Wts), which in turn phosphorylates and inactivates the Yorkie (Yki) transcriptional activator. The core fly components hippo, sav, wts and mats are conserved in mammals as STK4/3 (MST1/2), SAV1/WW45, LATS1/2 and MOB1. |
| mitotic cytokinesis | A cell cycle process that results in the division of the cytoplasm of a cell after mitosis, resulting in the separation of the original cell into two daughter cells. |
| positive regulation of protein phosphorylation | Any process that activates or increases the frequency, rate or extent of addition of phosphate groups to amino acids within a protein. |
| regulation of cytokinesis | Any process that modulates the frequency, rate or extent of the division of the cytoplasm of a cell and its separation into two daughter cells. |
| regulation of exit from mitosis | Any process involved in the progression from anaphase/telophase to G1 that is associated with a conversion from high to low mitotic CDK activity. |
| signal transduction | The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell. |
16 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P43563 | MOB2 | CBK1 kinase activator protein MOB2 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| Q95RA8 | mats | MOB kinase activator-like 1 | Drosophila melanogaster (Fruit fly) | SS |
| Q70IA8 | MOB3C | MOB kinase activator 3C | Homo sapiens (Human) | SS |
| Q96BX8 | MOB3A | MOB kinase activator 3A | Homo sapiens (Human) | SS |
| Q86TA1 | MOB3B | MOB kinase activator 3B | Homo sapiens (Human) | SS |
| Q9H8S9 | MOB1A | MOB kinase activator 1A | Homo sapiens (Human) | EV |
| Q7L9L4 | MOB1B | MOB kinase activator 1B | Homo sapiens (Human) | SS |
| Q8BJG4 | Mob3c | MOB kinase activator 3C | Mus musculus (Mouse) | SS |
| Q8BSU7 | Mob3a | MOB kinase activator 3A | Mus musculus (Mouse) | SS |
| Q8VE04 | Mob3b | MOB kinase activator 3B | Mus musculus (Mouse) | SS |
| Q8VI63 | Mob2 | MOB kinase activator 2 | Mus musculus (Mouse) | EV |
| Q921Y0 | Mob1a | MOB kinase activator 1A | Mus musculus (Mouse) | SS |
| Q8BPB0 | Mob1b | MOB kinase activator 1B | Mus musculus (Mouse) | EV |
| Q3T1J9 | Mob1a | MOB kinase activator 1A | Rattus norvegicus (Rat) | SS |
| Q8GYX0 | MOB1B | MOB kinase activator-like 1B | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9FHI1 | MOB1A | MOB kinase activator-like 1A | Arabidopsis thaliana (Mouse-ear cress) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSFLQNFHIS | PGQTIRSTRG | FKWNTANAAN | NAGSVSPTKA | TPHNNTINGN | NNNANTINNR |
| 70 | 80 | 90 | 100 | 110 | 120 |
| ADFTNNPVNG | YNESDHGRMS | PVLTTPKRHA | PPPEQLQNVT | DFNYTPSHQK | PFLQPQAGTT |
| 130 | 140 | 150 | 160 | 170 | 180 |
| VTTHQDIKQI | VEMTLGSEGV | LNQAVKLPRG | EDENEWLAVH | CVDFYNQINM | LYGSITEFCS |
| 190 | 200 | 210 | 220 | 230 | 240 |
| PQTCPRMIAT | NEYEYLWAFQ | KGQPPVSVSA | PKYVECLMRW | CQDQFDDESL | FPSKVTGTFP |
| 250 | 260 | 270 | 280 | 290 | 300 |
| EGFIQRVIQP | ILRRLFRVYA | HIYCHHFNEI | LELNLQTVLN | TSFRHFCLFA | QEFELLRPAD |
| 310 | |||||
| FGPLLELVME | LRDR |