P39073

PR
Gene name
SSN3 (ARE1, CDK8, GIG2, SRB10, UME5)
Protein name
Meiotic mRNA stability protein kinase SSN3
Names
Cyclin-dependent kinase 8, Suppressor of RNA polymerase B SRB10
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YPL042C
EC number
2.7.11.22: Protein-serine/threonine kinases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-196 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

303-329 (Activation loop from InterPro)

Target domain

75-463 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

3 structures for P39073

Entry ID Method Resolution Chain Position Source
7KPV EM 380 A A 1-555 PDB
7KPX EM 440 A A 1-555 PDB
AF-P39073-F1 Predicted AlphaFoldDB

7 variants for P39073

Variant ID(s) Position Change Description Diseaes Association Provenance
s16-474585 40 S>R No SGRP
s16-474523 61 R>K No SGRP
s16-474367 113 A>G No SGRP
s16-474368 113 A>T No SGRP
s16-473816 297 D>N No SGRP
s16-473509 399 I>T No SGRP
s16-473467 413 T>R No SGRP

No associated diseases with P39073

2 regional properties for P39073

Type Name Position InterPro Accession
domain Protein kinase domain 75 - 463 IPR000719
active_site Serine/threonine-protein kinase, active site 282 - 294 IPR008271

Functions

Description
EC Number 2.7.11.22 Protein-serine/threonine kinases
Subcellular Localization
  • Nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
CKM complex Cyclin-dependent kinase complex which reversibly associates with the Mediator complex. In Saccharomyces cerevisiae it consists of SSN2, SSN3, SSN8 and SRB8.
mediator complex A protein complex that interacts with the carboxy-terminal domain of the largest subunit of RNA polymerase II and plays an active role in transducing the signal from a transcription factor to the transcriptional machinery. The mediator complex is required for activation of transcription of most protein-coding genes, but can also act as a transcriptional corepressor. The Saccharomyces complex contains several identifiable subcomplexes: a head domain comprising Srb2, -4, and -5, Med6, -8, and -11, and Rox3 proteins; a middle domain comprising Med1, -4, and -7, Nut1 and -2, Cse2, Rgr1, Soh1, and Srb7 proteins; a tail consisting of Gal11p, Med2p, Pgd1p, and Sin4p; and a regulatory subcomplex comprising Ssn2, -3, and -8, and Srb8 proteins. Metazoan mediator complexes have similar modular structures and include homologs of yeast Srb and Med proteins.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

6 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
cyclin-dependent protein serine/threonine kinase activity Cyclin-dependent catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
metal ion binding Binding to a metal ion.
protein kinase activity Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
RNA polymerase II CTD heptapeptide repeat kinase activity Catalysis of the reaction: ATP + RNA polymerase II large subunit CTD heptapeptide repeat (YSPTSPS) = ADP + H+ + phosphorylated RNA polymerase II.

9 GO annotations of biological process

Name Definition
meiotic cell cycle Progression through the phases of the meiotic cell cycle, in which canonically a cell replicates to produce four offspring with half the chromosomal content of the progenitor cell via two nuclear divisions.
negative regulation of filamentous growth Any process that decreases the frequency, rate or extent of the process in which a multicellular organism or a group of unicellular organisms grow in a threadlike, filamentous shape.
negative regulation of transcription by RNA polymerase II Any process that stops, prevents, or reduces the frequency, rate or extent of transcription mediated by RNA polymerase II.
nuclear-transcribed mRNA catabolic process, non-stop decay The chemical reactions and pathways resulting in the breakdown of the transcript body of a nuclear-transcribed mRNA that is lacking a stop codon.
phosphorylation of RNA polymerase II C-terminal domain The process of introducing a phosphate group on to an amino acid residue in the C-terminal domain of RNA polymerase II. Typically, this occurs during the transcription cycle and results in production of an RNA polymerase II enzyme where the carboxy-terminal domain (CTD) of the largest subunit is extensively phosphorylated, often referred to as hyperphosphorylated or the II(0) form. Specific types of phosphorylation within the CTD are usually associated with specific regions of genes, though there are exceptions. The phosphorylation state regulates the association of specific complexes such as the capping enzyme or 3'-RNA processing machinery to the elongating RNA polymerase complex.
positive regulation of transcription from RNA polymerase II promoter by galactose Any process involving galactose that activates or increases the rate of transcription from an RNA polymerase II promoter.
protein destabilization Any process that decreases the stability of a protein, making it more vulnerable to degradative processes or aggregation.
protein phosphorylation The process of introducing a phosphate group on to a protein.
response to oxidative stress Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of oxidative stress, a state often resulting from exposure to high levels of reactive oxygen species, e.g. superoxide anions, hydrogen peroxide (H2O2), and hydroxyl radicals.

8 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q03957 CTK1 CTD kinase subunit alpha Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
Q9VT57 Cdk8 Cyclin-dependent kinase 8 Drosophila melanogaster (Fruit fly) PR
Q9BWU1 CDK19 Cyclin-dependent kinase 19 Homo sapiens (Human) PR
P49336 CDK8 Cyclin-dependent kinase 8 Homo sapiens (Human) PR
Q8BWD8 Cdk19 Cyclin-dependent kinase 19 Mus musculus (Mouse) PR
Q8R3L8 Cdk8 Cyclin-dependent kinase 8 Mus musculus (Mouse) PR
Q6P3N6 cdk8 Cyclin-dependent kinase 8 Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) PR
Q8JH47 cdk8 Cyclin-dependent kinase 8 Danio rerio (Zebrafish) (Brachydanio rerio) PR
10 20 30 40 50 60
MYNGKDRAQN SYQPMYQRPM QVQGQQQAQS FVGKKNTIGS VHGKAPMLMA NNDVFTIGPY
70 80 90 100 110 120
RARKDRMRVS VLEKYEVIGY IAAGTYGKVY KAKRQINSGT NSANGSSLNG TNAKIPQFDS
130 140 150 160 170 180
TQPKSSSSMD MQANTNALRR NLLKDEGVTP GRIRTTREDV SPHYNSQKQT LIKKPLTVFY
190 200 210 220 230 240
AIKKFKTEKD GVEQLHYTGI SQSACREMAL CRELHNKHLT TLVEIFLERK CVHMVYEYAE
250 260 270 280 290 300
HDLLQIIHFH SHPEKRMIPP RMVRSIMWQL LDGVSYLHQN WVLHRDLKPA NIMVTIDGCV
310 320 330 340 350 360
KIGDLGLARK FHNMLQTLYT GDKVVVTIWY RAPELLLGAR HYTPAVDLWS VGCIFAELIG
370 380 390 400 410 420
LQPIFKGEEA KLDSKKTVPF QVNQLQRILE VLGTPDQKIW PYLEKYPEYD QITKFPKYRD
430 440 450 460 470 480
NLATWYHSAG GRDKHALSLL YHLLNYDPIK RIDAFNALEH KYFTESDIPV SENVFEGLTY
490 500 510 520 530 540
KYPARRIHTN DNDIMNLGSR TKNNTQASGI TAGAAANALG GLGVNRRILA AAAAAAAAVS
550
GNNASDEPSR KKNRR