P36887

PR
Gene name
PRKACA
Protein name
cAMP-dependent protein kinase catalytic subunit alpha
Names
PKA C-alpha
Species
Sus scrofa (Pig)
KEGG Pathway
EC number
2.7.11.11: Protein-serine/threonine kinases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-61 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

184-204 (Activation loop from InterPro)

Target domain

44-298 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

4 structures for P36887

Entry ID Method Resolution Chain Position Source
1CDK X-ray 200 A A/B 2-351 PDB
1CMK X-ray 290 A E 2-351 PDB
1CTP X-ray 290 A E 2-351 PDB
AF-P36887-F1 Predicted AlphaFoldDB

No variants for P36887

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P36887

No associated diseases with P36887

4 regional properties for P36887

Type Name Position InterPro Accession
domain Protein kinase domain 44 - 298 IPR000719
domain AGC-kinase, C-terminal 299 - 351 IPR000961
active_site Serine/threonine-protein kinase, active site 163 - 175 IPR008271
binding_site Protein kinase, ATP binding site 50 - 73 IPR017441

Functions

Description
EC Number 2.7.11.11 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Cell membrane
  • Membrane ; Lipid-anchor
  • Nucleus
  • Mitochondrion
  • Translocates into the nucleus (monomeric catalytic subunit)
  • The inactive holoenzyme is found in the cytoplasm
  • Distributed throughout the cytoplasm in meiotically incompetent oocytes
  • Associated to mitochondrion as meiotic competence is acquired
  • Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes (By similarity)
  • Colocalizes with HSF1 in nuclear stress bodies (nSBs) upon heat shock (By similarity)
  • Recruited to the cell membrane through interaction with SMO (By similarity)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

8 GO annotations of cellular component

Name Definition
acrosomal vesicle A structure in the head of a spermatozoon that contains acid hydrolases, and is concerned with the breakdown of the outer membrane of the ovum during fertilization. It lies just beneath the plasma membrane and is derived from the lysosome.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
germinal vesicle The enlarged, fluid filled nucleus of a primary oocyte, the development of which is suspended in prophase I of the first meiotic division between embryohood and sexual maturity.
mitochondrion A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
perinuclear region of cytoplasm Cytoplasm situated near, or occurring around, the nucleus.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
sperm flagellum A microtubule-based flagellum (or cilium) that is part of a sperm, a mature male germ cell that develops from a spermatid.

5 GO annotations of molecular function

Name Definition
AMP-activated protein kinase activity Catalysis of the reaction: ATP + a protein = ADP + a phosphoprotein. This reaction requires the presence of AMP.
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
cAMP-dependent protein kinase activity cAMP-dependent catalysis of the reaction: ATP + a protein = ADP + a phosphoprotein.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.

3 GO annotations of biological process

Name Definition
cellular response to heat Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a heat stimulus, a temperature stimulus above the optimal temperature for that organism.
negative regulation of meiotic cell cycle process involved in oocyte maturation Any process that stops, prevents or reduces the frequency, rate or extent of meiotic cell cycle process involved in oocyte maturation.
protein phosphorylation The process of introducing a phosphate group on to a protein.

10 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P00517 PRKACA cAMP-dependent protein kinase catalytic subunit alpha Bos taurus (Bovine) PR
Q8MJ44 PRKACA cAMP-dependent protein kinase catalytic subunit alpha Canis lupus familiaris (Dog) (Canis familiaris) PR
Q03043 for cGMP-dependent protein kinase, isozyme 2 forms cD4/T1/T3A/T3B Drosophila melanogaster (Fruit fly) SS
P16911 Pka-C2 cAMP-dependent protein kinase catalytic subunit 2 Drosophila melanogaster (Fruit fly) PR
P17612 PRKACA cAMP-dependent protein kinase catalytic subunit alpha Homo sapiens (Human) PR
Q922R0 Prkx cAMP-dependent protein kinase catalytic subunit PRKX Mus musculus (Mouse) PR
P05132 Prkaca cAMP-dependent protein kinase catalytic subunit alpha Mus musculus (Mouse) PR
O62846 PRKACG cAMP-dependent protein kinase catalytic subunit gamma Macaca mulatta (Rhesus macaque) PR
Q7JP68 F47F2.1 cAMP-dependent protein kinase, catalytic subunit-like Caenorhabditis elegans PR
P21137 kin-1 cAMP-dependent protein kinase catalytic subunit Caenorhabditis elegans PR
10 20 30 40 50 60
MGNAAAAKKG SEQESVKEFL AKAKEDFLKK WENPAQNTAH LDQFERIKTL GTGSFGRVML
70 80 90 100 110 120
VKHKETGNHF AMKILDKQKV VKLKQIEHTL NEKRILQAVN FPFLVKLEYS FKDNSNLYMV
130 140 150 160 170 180
MEYVPGGEMF SHLRRIGRFS EPHARFYAAQ IVLTFEYLHS LDLIYRDLKP ENLLIDQQGY
190 200 210 220 230 240
IQVTDFGFAK RVKGRTWTLC GTPEYLAPEI ILSKGYNKAV DWWALGVLIY EMAAGYPPFF
250 260 270 280 290 300
ADQPIQIYEK IVSGKVRFPS HFSSDLKDLL RNLLQVDLTK RFGNLKNGVN DIKNHKWFAT
310 320 330 340 350
TDWIAIYQRK VEAPFIPKFK GPGDTSNFDD YEEEEIRVSI NEKCGKEFSE F