P35761

SS
Gene name
Ttk (Esk)
Protein name
Dual specificity protein kinase TTK
Names
EC 2.7.12.1 , ESK , PYT
Species
Mus musculus (Mouse)
KEGG Pathway
EC number
2.7.12.1: Dual-specificity kinases (those acting on Ser/Thr and Tyr residues)
Protein Class

Descriptions

Monopolar spindle 1 (MPS1 or TTK) is a conserved apical kinase in the spindle assembly checkpoint (SAC) that ensures accurate segregation of chromosomes during mitosis. MPS1 undergoes extensive auto- and transphosphorylation. The N-terminal extension harbors multiple phosphorylation sites and is involved in dimerization and catalytic activation. Phosphorylation within the N-terminal extension contributes to MPS1 activation through relief of catalytic autoinhibition that is mediated by the N-terminal extension itself.

Autoinhibitory domains (AIDs)

39-45 (N-terminal extension) SS

Target domain

524-790 (Protein kinase domain)

Relief mechanism

PTM

Assay

Accessory elements

662-687 (Activation loop from InterPro)

Target domain

524-790 (Protein kinase domain)

Relief mechanism

Assay

662-687 (Activation loop from InterPro)

Target domain

522-790 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for P35761

Entry ID Method Resolution Chain Position Source
AF-P35761-F1 Predicted AlphaFoldDB

No variants for P35761

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P35761

No associated diseases with P35761

3 regional properties for P35761

Type Name Position InterPro Accession
domain Protein kinase domain 524 - 790 IPR000719
active_site Serine/threonine-protein kinase, active site 642 - 654 IPR008271
domain Protein kinase Mps1 family, catalytic domain 522 - 790 IPR027084

Functions

Description
EC Number 2.7.12.1 Dual-specificity kinases (those acting on Ser/Thr and Tyr residues)
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
kinetochore A multisubunit complex that is located at the centromeric region of DNA and provides an attachment point for the spindle microtubules.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

7 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
identical protein binding Binding to an identical protein or proteins.
kinetochore binding Binding to a kinetochore, a proteinaceous structure on a condensed chromosome, beside the centromere, to which the spindle fibers are attached.
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions
protein serine/threonine/tyrosine kinase activity Catalysis of the reactions
protein tyrosine kinase activity Catalysis of the reaction

8 GO annotations of biological process

Name Definition
chromosome segregation The process in which genetic material, in the form of chromosomes, is organized into specific structures and then physically separated and apportioned to two or more sets. In eukaryotes, chromosome segregation begins with the condensation of chromosomes, includes chromosome separation, and ends when chromosomes have completed movement to the spindle poles.
female meiosis chromosome segregation The cell cycle process in which genetic material, in the form of chromosomes, is organized and then physically separated and apportioned to two or more sets during the meiotic cell cycle in a female.
meiotic spindle assembly checkpoint signaling A signal transduction process that contributes to a meiotic spindle assembly checkpoint, that delays the metaphase/anaphase transition of a meiotic cell cycle until the spindle is correctly assembled and chromosomes are attached to the spindle.
mitotic spindle assembly checkpoint signaling A mitotic cell cycle checkpoint that delays mitotic sister chromatid separation and consequently the mitotic metaphase/anaphase transition until the spindle is correctly assembled and chromosomes are attached to the spindle. Spindle assembly checkpoint signalling begins with the activated Mph family kinase, and results in the inhibition of the Anaphase Promoting Complex and its activator Sleepy/Cdc20 by the mitotic checkpoint complex (MCC).
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
protein localization to chromosome Any process in which a protein is transported to, or maintained at, a specific location on a chromosome.
protein localization to kinetochore Any process in which a protein is transported to, or maintained at, the kinetochore.
protein localization to meiotic spindle midzone A process in which a protein is transported to, or maintained in, a location within a meiotic spindle midzone.

3 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P33981 TTK Dual specificity protein kinase TTK Homo sapiens (Human) EV
Q84VX4 MPS1 Serine/threonine-protein kinase MPS1 Arabidopsis thaliana (Mouse-ear cress) SS
Q8AYG3 ttk Dual specificity protein kinase Ttk Danio rerio (Zebrafish) (Brachydanio rerio) PR
10 20 30 40 50 60
MEAEELIGSS VTIDSIMSKM RDIKNKINED CTDELSLSKI CADHTETVNQ IMRVGNTPEN
70 80 90 100 110 120
WLNFLLKLEK NSSPLNDDLL NKLIGRYSQA IEVLPPDKYG QNESFARIQV RLAELKAIQE
130 140 150 160 170 180
PDDARDYFQM ARENCKKFAF VHVSFAQFEL SQGNLKKSEQ LLHKAVETGA VPLQMLETAM
190 200 210 220 230 240
RNLHLQKKQL LPEEDKKSVS ASTVLSAQEP FSSSLGNVQN RSISCESRGQ AGAARVLYGE
250 260 270 280 290 300
NLPPQDAEVR HQNPFKQTHA AKRSCPFGRV PVNLLNSPDF YVKTDSSAVT QLTTRLALSS
310 320 330 340 350 360
VPLPYVTCLL HLQLLALAGL AKGSGPDRDA ILPGSRPRGS DSYELRGLKP IQTIYLKDSL
370 380 390 400 410 420
VSNEKSSELM SDLIALKSKT DSSLTKLEET KPEIAERRPM QWQSTRKPEC VFQNPAAFAP
430 440 450 460 470 480
LRHVPDVTPK ADKESPPISV PKWLDPKSAC ETPSSSSLDD YMKCFKTPVV KNDFPPACPS
490 500 510 520 530 540
STPYSQLARL QQQQQQGLST PLQSLQISGS SSINECISVN GRIYSILKQI GSGGSSKVFQ
550 560 570 580 590 600
VLNEKKQINA IKYVNLEDAD SQTIESYRNE IAFLNKLQQH SDKIIRLYDY EITEQYIYMV
610 620 630 640 650 660
MECGNIDLNS WLKKKKSINP WERKSYWKNM LEAVHIIHQH GIVHSDLKPA NFVIVDGMLK
670 680 690 700 710 720
LIDFGIANQM QPDTTSIVKD SQVGTVNYMA PEAIRDMSSS RENSKIRTKV SPRSDVWSLG
730 740 750 760 770 780
CILYYMTYGR TPFQHIINQV SKLHAIINPA HEIEFPEISE KDLRDVLKCC LVRNPKERIS
790 800 810 820 830 840
IPELLTHPYV QIQPHPGSQM ARGATDEMKY VLGQLVGLNS PNSILKTAKT LYERYNCGEG
850
QDSSSSKTFD KKRERK