Descriptions
Autoinhibitory domains (AIDs)
Target domain |
9-337 (Kinesin motor domain) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
No accessory elements
References
- Chiba K et al. (2022) "Synergistic autoinhibition and activation mechanisms control kinesin-1 motor activity", Cell reports, 39, 110900
- Dietrich KA et al. (2008) "The kinesin-1 motor protein is regulated by a direct interaction of its head and tail", Proceedings of the National Academy of Sciences of the United States of America, 105, 8938-43
- Friedman DS et al. (1999) "Single-molecule analysis of kinesin motility reveals regulation by the cargo-binding tail domain", Nature cell biology, 1, 293-7
- Sindelar CV et al. (2002) "Two conformations in the human kinesin power stroke defined by X-ray crystallography and EPR spectroscopy", Nature structural biology, 9, 844-8
- Kaan HY et al. (2011) "The structure of the kinesin-1 motor-tail complex reveals the mechanism of autoinhibition", Science (New York, N.Y.), 333, 883-5
- Korten T et al. (2018) "An automated in vitro motility assay for high-throughput studies of molecular motors", Lab on a chip, 18, 3196-3206
- Hannaford MR et al. (2022) "Pericentrin interacts with Kinesin-1 to drive centriole motility", The Journal of cell biology, 221,
- Yeon JH et al. (2016) "Systems-wide Identification of cis-Regulatory Elements in Proteins", Cell systems, 2, 89-100
Autoinhibited structure
Activated structure
1 structures for P34540
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-P34540-F1 | Predicted | AlphaFoldDB |
No variants for P34540
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for P34540 | |||||
No associated diseases with P34540
9 GO annotations of cellular component
| Name | Definition |
|---|---|
| axon cytoplasm | Any cytoplasm that is part of a axon. |
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| dendrite cytoplasm | All of the contents of a dendrite, excluding the surrounding plasma membrane. |
| kinesin complex | Any complex that includes a dimer of molecules from the kinesin superfamily, a group of related proteins that contain an extended region of predicted alpha-helical coiled coil in the main chain that likely produces dimerization. The native complexes of several kinesin family members have also been shown to contain additional peptides, often designated light chains as all of the noncatalytic subunits that are currently known are smaller than the chain that contains the motor unit. Kinesin complexes generally possess a force-generating enzymatic activity, or motor, which converts the free energy of the gamma phosphate bond of ATP into mechanical work. |
| kinesin I complex | A complex of two kinesin heavy chains and two kinesin light chains. |
| microtubule | Any of the long, generally straight, hollow tubes of internal diameter 12-15 nm and external diameter 24 nm found in a wide variety of eukaryotic cells; each consists (usually) of 13 protofilaments of polymeric tubulin, staggered in such a manner that the tubulin monomers are arranged in a helical pattern on the microtubular surface, and with the alpha/beta axes of the tubulin subunits parallel to the long axis of the tubule; exist in equilibrium with pool of tubulin monomers and can be rapidly assembled or disassembled in response to physiological stimuli; concerned with force generation, e.g. in the spindle. |
| neuron projection | A prolongation or process extending from a nerve cell, e.g. an axon or dendrite. |
| nuclear envelope | The double lipid bilayer enclosing the nucleus and separating its contents from the rest of the cytoplasm; includes the intermembrane space, a gap of width 20-40 nm (also called the perinuclear space). |
| synapse | The junction between an axon of one neuron and a dendrite of another neuron, a muscle fiber or a glial cell. As the axon approaches the synapse it enlarges into a specialized structure, the presynaptic terminal bouton, which contains mitochondria and synaptic vesicles. At the tip of the terminal bouton is the presynaptic membrane; facing it, and separated from it by a minute cleft (the synaptic cleft) is a specialized area of membrane on the receiving cell, known as the postsynaptic membrane. In response to the arrival of nerve impulses, the presynaptic terminal bouton secretes molecules of neurotransmitters into the synaptic cleft. These diffuse across the cleft and transmit the signal to the postsynaptic membrane. |
4 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| ATP hydrolysis activity | Catalysis of the reaction |
| microtubule binding | Binding to a microtubule, a filament composed of tubulin monomers. |
| plus-end-directed microtubule motor activity | A motor activity that generates movement along a microtubule toward the plus end, driven by ATP hydrolysis. |
18 GO annotations of biological process
| Name | Definition |
|---|---|
| anterograde axonal transport of mitochondrion | The directed movement of mitochondria along microtubules in axons away from the cell body and towards the presynapse. |
| anterograde dendritic transport of neurotransmitter receptor complex | The directed movement of a neurotransmitter receptor complex along microtubules in nerve cell dendrites towards the postsynapse. |
| axon guidance | The chemotaxis process that directs the migration of an axon growth cone to a specific target site in response to a combination of attractive and repulsive cues. |
| embryo development ending in birth or egg hatching | The process whose specific outcome is the progression of an embryo over time, from zygote formation until the end of the embryonic life stage. The end of the embryonic life stage is organism-specific and may be somewhat arbitrary; for mammals it is usually considered to be birth, for insects the hatching of the first instar larva from the eggshell. |
| establishment of meiotic spindle localization | The cell cycle process in which the directed movement of the meiotic spindle to a specific location in the cell occurs. |
| establishment of meiotic spindle orientation | Any process that set the alignment of meiotic spindle relative to other cellular structures. |
| establishment or maintenance of microtubule cytoskeleton polarity | Any cellular process that results in the specification, formation or maintenance of polarized microtubule-based cytoskeletal structures. |
| locomotion | Self-propelled movement of a cell or organism from one location to another. |
| meiotic cytokinesis | A cell cycle process that results in the division of the cytoplasm of a cell after meiosis, resulting in the separation of the original cell into two daughter cells. |
| mitotic cell cycle | Progression through the phases of the mitotic cell cycle, the most common eukaryotic cell cycle, which canonically comprises four successive phases called G1, S, G2, and M and includes replication of the genome and the subsequent segregation of chromosomes into daughter cells. In some variant cell cycles nuclear replication or nuclear division may not be followed by cell division, or G1 and G2 phases may be absent. |
| necroptotic process | A programmed necrotic cell death process which begins when a cell receives a signal (e.g. a ligand binding to a death receptor or to a Toll-like receptor), and proceeds through a series of biochemical events (signaling pathways), characterized by activation of receptor-interacting serine/threonine-protein kinase 1 and/or 3 (RIPK1/3, also called RIP1/3) and by critical dependence on mixed lineage kinase domain-like (MLKL), and which typically lead to common morphological features of necrotic cell death. The process ends when the cell has died. The process is divided into a signaling phase, and an execution phase, which is triggered by the former. |
| nuclear migration along microtubule | The directed movement of the nucleus along microtubules within the cell, mediated by motor proteins. |
| polar body extrusion after meiotic divisions | The cell cycle process in which two small cells are generated, as byproducts destined to degenerate, as a result of the first and second meiotic divisions of a primary oocyte during its development to a mature ovum. One polar body is formed in the first division of meiosis and the other in the second division; at each division, the cytoplasm divides unequally, so that the polar body is of much smaller size than the developing oocyte. At the second division in which a polar body is formed, the polar body and the developing oocyte each contain a haploid set of chromosomes. |
| positive regulation of cilium assembly | Any process that activates or increases the frequency, rate or extent of the formation of a cilium. |
| positive regulation of dendrite morphogenesis | Any process that activates or increases the frequency, rate or extent of dendrite morphogenesis. |
| protein localization | Any process in which a protein is transported to, or maintained in, a specific location. |
| regulation of dendrite morphogenesis | Any process that modulates the frequency, rate or extent of dendrite morphogenesis. |
| synaptic vesicle transport | The directed movement of synaptic vesicles. |
11 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P17210 | Khc | Kinesin heavy chain | Drosophila melanogaster (Fruit fly) | EV |
| P33176 | KIF5B | Kinesin-1 heavy chain | Homo sapiens (Human) | EV |
| Q12840 | KIF5A | Kinesin heavy chain isoform 5A | Homo sapiens (Human) | EV |
| O60282 | KIF5C | Kinesin heavy chain isoform 5C | Homo sapiens (Human) | EV |
| P28738 | Kif5c | Kinesin heavy chain isoform 5C | Mus musculus (Mouse) | SS |
| P33175 | Kif5a | Kinesin heavy chain isoform 5A | Mus musculus (Mouse) | SS |
| Q61768 | Kif5b | Kinesin-1 heavy chain | Mus musculus (Mouse) | EV |
| Q2PQA9 | Kif5b | Kinesin-1 heavy chain | Rattus norvegicus (Rat) | SS |
| Q6QLM7 | Kif5a | Kinesin heavy chain isoform 5A | Rattus norvegicus (Rat) | SS |
| G5EFQ4 | klp-6 | Kinesin-like protein | Caenorhabditis elegans | EV |
| Q9SV36 | KINUC | Kinesin-like protein KIN-UC | Arabidopsis thaliana (Mouse-ear cress) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MEPRTDGAEC | GVQVFCRIRP | LNKTEEKNAD | RFLPKFPSED | SISLGGKVYV | FDKVFKPNTT |
| 70 | 80 | 90 | 100 | 110 | 120 |
| QEQVYKGAAY | HIVQDVLSGY | NGTVFAYGQT | SSGKTHTMEG | VIGDNGLSGI | IPRIVADIFN |
| 130 | 140 | 150 | 160 | 170 | 180 |
| HIYSMDENLQ | FHIKVSYYEI | YNEKIRDLLD | PEKVNLSIHE | DKNRVPYVKG | ATERFVGGPD |
| 190 | 200 | 210 | 220 | 230 | 240 |
| EVLQAIEDGK | SNRMVAVTNM | NEHSSRSHSV | FLITVKQEHQ | TTKKQLTGKL | YLVDLAGSEK |
| 250 | 260 | 270 | 280 | 290 | 300 |
| VSKTGAQGTV | LEEAKNINKS | LTALGIVISA | LAEGTKSHVP | YRDSKLTRIL | QESLGGNSRT |
| 310 | 320 | 330 | 340 | 350 | 360 |
| TVIICASPSH | FNEAETKSTL | LFGARAKTIK | NVVQINEELT | AEEWKRRYEK | EKEKNTRLAA |
| 370 | 380 | 390 | 400 | 410 | 420 |
| LLQAAALELS | RWRAGESVSE | VEWVNLSDSA | QMAVSEVSGG | STPLMERSIA | PAPPMLTSTT |
| 430 | 440 | 450 | 460 | 470 | 480 |
| GPITDEEKKK | YEEERVKLYQ | QLDEKDDEIQ | KVSQELEKLR | QQVLLQEEAL | GTMRENEELI |
| 490 | 500 | 510 | 520 | 530 | 540 |
| REENNRFQKE | AEDKQQEGKE | MMTALEEIAV | NLDVRQAECE | KLKRELEVVQ | EDNQSLEDRM |
| 550 | 560 | 570 | 580 | 590 | 600 |
| NQATSLLNAH | LDECGPKIRH | FKEGIYNVIR | EFNIADIASQ | NDQLPDHDLL | NHVRIGVSKL |
| 610 | 620 | 630 | 640 | 650 | 660 |
| FSEYSAAKES | STAAEHDAEA | KLAADVARVE | SGQDAGRMKQ | LLVKDQAAKE | IKPLTDRVNM |
| 670 | 680 | 690 | 700 | 710 | 720 |
| ELTTLKNLKK | EFMRVLVARC | QANQDTEGED | SLSGPAQKQR | IQFLENNLDK | LTKVHKQLVR |
| 730 | 740 | 750 | 760 | 770 | 780 |
| DNADLRVELP | KMEARLRGRE | DRIKILETAL | RDSKQRSQAE | RKKYQQEVER | IKEAVRQRNM |
| 790 | 800 | 810 | |||
| RRMNAPQIVK | PIRPGQVYTS | PSAGMSQGAP | NGSNA |