P34331

SS
Gene name
plk-1 (plc1, C14B9.4)
Protein name
Serine/threonine-protein kinase plk-1
Names
EC 2.7.11.21 , Polo-like kinase 1
Species
Caenorhabditis elegans
KEGG Pathway
cel:CELE_C14B9.4
EC number
2.7.11.21: Protein-serine/threonine kinases
Protein Class

Descriptions

Polo-like kinase Plk1 acts at various stages in the cell cycle, specifically mitosis and cytokinesis. The kinase activity is regulated by a conserved C-terminal domain, termed the polo box domain (PBD). PBD is composed of the two polo boxes and an N-terminal extension, and each polo box contains a continuous six-stranded antiparallel β-sheet and an α-helix. The C-terminal region containing a polo-box domain is involved in autoinhibition via intramolecular interaction with the kinase domain in PLK1.

Autoinhibitory domains (AIDs)

368-649 (Polo box domain) SS

Target domain

38-290 (Protein kinase domain)

Relief mechanism

PTM

Assay

Accessory elements

179-201 (Activation loop from InterPro)

Target domain

38-290 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for P34331

Entry ID Method Resolution Chain Position Source
AF-P34331-F1 Predicted AlphaFoldDB

No variants for P34331

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for P34331

No associated diseases with P34331

7 regional properties for P34331

Type Name Position InterPro Accession
domain Protein kinase domain 38 - 290 IPR000719
domain POLO box domain 413 - 493 IPR000959-1
domain POLO box domain 515 - 597 IPR000959-2
active_site Serine/threonine-protein kinase, active site 158 - 170 IPR008271
binding_site Protein kinase, ATP binding site 44 - 67 IPR017441
domain Second polo-box domain 514 - 595 IPR033695
domain First polo-box domain 410 - 500 IPR033701

Functions

Description
EC Number 2.7.11.21 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
  • Midbody
  • Cytoplasm
  • Nucleus
  • Chromosome
  • Chromosome, centromere, kinetochore
  • In mitosis, remains associated with centrosomes entering prophase through to anaphase
  • During metaphase, embryos show anterior enrichment and located to the chromosomes of the metaphase plate
  • In meiosis, detected at centrosomes after pronuclear meeting in post-meiotic 1-cell embryos
  • Associated with chromatin during chromosome segregation of anaphase and in the region between the dividing chromosomes
  • Cytoplasmic in mature, unfertilized oocytes (PubMed:10660671)
  • Asymmetric cytoplasmic localization is regulated by mex-5 and mex-6 (PubMed:18199581, PubMed:18316412)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

8 GO annotations of cellular component

Name Definition
centrosome A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
condensed chromosome A highly compacted molecule of DNA and associated proteins resulting in a cytologically distinct structure.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
kinetochore A multisubunit complex that is located at the centromeric region of DNA and provides an attachment point for the spindle microtubules.
midbody A thin cytoplasmic bridge formed between daughter cells at the end of cytokinesis. The midbody forms where the contractile ring constricts, and may persist for some time before finally breaking to complete cytokinesis.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
spindle midzone The area in the center of the spindle where the spindle microtubules from opposite poles overlap.
spindle pole Either of the ends of a spindle, where spindle microtubules are organized; usually contains a microtubule organizing center and accessory molecules, spindle microtubules and astral microtubules.

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
protein kinase activity Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions

9 GO annotations of biological process

Name Definition
centrosome cycle The cell cycle process in which centrosome duplication and separation takes place. The centrosome cycle can operate with a considerable degree of independence from other processes of the cell cycle.
female meiosis II The cell cycle process in which the second meiotic division occurs in the female germline.
meiotic chromosome segregation The process in which genetic material, in the form of chromosomes, is organized into specific structures and then physically separated and apportioned to two or more sets during M phase of the meiotic cell cycle.
mitotic nuclear membrane disassembly The mitotic cell cycle process in which the controlled partial or complete breakdown of the nuclear membranes during occurs during mitosis.
mitotic spindle organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the microtubule spindle during a mitotic cell cycle.
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
polar body extrusion after meiotic divisions The cell cycle process in which two small cells are generated, as byproducts destined to degenerate, as a result of the first and second meiotic divisions of a primary oocyte during its development to a mature ovum. One polar body is formed in the first division of meiosis and the other in the second division; at each division, the cytoplasm divides unequally, so that the polar body is of much smaller size than the developing oocyte. At the second division in which a polar body is formed, the polar body and the developing oocyte each contain a haploid set of chromosomes.
positive regulation of nematode male tail tip morphogenesis Any process that activates or increases the frequency, rate or extent of nematode male tail tip morphogenesis.
regulation of cell cycle Any process that modulates the rate or extent of progression through the cell cycle.

9 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q2TA25 PLK1 Serine/threonine-protein kinase PLK1 Bos taurus (Bovine) SS
O97143 SAK Serine/threonine-protein kinase PLK4 Drosophila melanogaster (Fruit fly) EV
P52304 polo Serine/threonine-protein kinase polo Drosophila melanogaster (Fruit fly) SS
P53350 PLK1 Serine/threonine-protein kinase PLK1 Homo sapiens (Human) EV
Q07832 Plk1 Serine/threonine-protein kinase PLK1 Mus musculus (Mouse) EV
Q62673 Plk1 Serine/threonine-protein kinase PLK1 Rattus norvegicus (Rat) SS
Q20845 plk-3 Serine/threonine-protein kinase plk-3 Caenorhabditis elegans PR
Q9N2L7 plk-2 Serine/threonine-protein kinase plk-2 Caenorhabditis elegans SS
P62205 plk1 Serine/threonine-protein kinase PLK1 Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) SS
10 20 30 40 50 60
MNRLPNIAKP PQKSNQRKEK APPEVPALIA DKDRGTYYEK GRFLGKGGFA HCYELTNRAT
70 80 90 100 110 120
REVVAGKVVP KSMLVKQYQR DKVDNERILI HRELGHINIV KLFNFFEDNL NVYITLELCA
130 140 150 160 170 180
RRSLMELHKR RKAVTEPEAR YFTHQIVDGV LYLHDLNIIH RDMKLGNLFL NDDLVVKIGD
190 200 210 220 230 240
FGLATTVNGD ERKKTLCGTP NYIAPEVLNK AGHSFEVDIW AVGCILYILL FGQPPFESKS
250 260 270 280 290 300
LEETYSRIRH NNYTIPSIAT QPAASLIRKM LDPEPTRRPT AKQVQRDGFF KSGFMPTRLP
310 320 330 340 350 360
VSCLTMVPKF GGHETSMMEE NVAPRGVDAR SQRPLNGRAG LSALPQHIVS NNADRERAQQ
370 380 390 400 410 420
QAAEATFREP EDAYLSQLFH QVAVLLEQRI PGLEEEEAAL DGYQSPECLP VFWISKWVDY
430 440 450 460 470 480
SDKYGIGYQL CDNSVGVLFN DNSRIMLDQA GNELTYIEKS NKEHYFSMHS GEMPGLLNKK
490 500 510 520 530 540
VTLLKYFRSY MNDHLVKAGE GSEQRAGDDL ARLPTLRVWF RTKSAIVLHL SNGTVQINFF
550 560 570 580 590 600
NDHVKMMMCP LMQAVTFIDQ NKRMLTYKLN NLQRNGCPEK FLHRLKYAKT MIERLMSDAN
610 620 630 640
VVSQNPARQP DMPRSMAAAR SASAGSRGPN QAASHLPQSA SGSNIHPRR