P33175
SS
Gene name |
Kif5a (Kiaa4086, Kif5, Nkhc1) |
Protein name |
Kinesin heavy chain isoform 5A |
Names |
Kinesin heavy chain neuron-specific 1 , Neuronal kinesin heavy chain , NKHC |
Species |
Mus musculus (Mouse) |
KEGG Pathway |
mmu:16572 |
EC number |
|
Protein Class |
|
Descriptions
Autoinhibitory domains (AIDs)
Target domain |
7-335 (Kinesin motor domain) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
No accessory elements
References
- Chiba K et al. (2022) "Synergistic autoinhibition and activation mechanisms control kinesin-1 motor activity", Cell reports, 39, 110900
- Dietrich KA et al. (2008) "The kinesin-1 motor protein is regulated by a direct interaction of its head and tail", Proceedings of the National Academy of Sciences of the United States of America, 105, 8938-43
- Friedman DS et al. (1999) "Single-molecule analysis of kinesin motility reveals regulation by the cargo-binding tail domain", Nature cell biology, 1, 293-7
- Sindelar CV et al. (2002) "Two conformations in the human kinesin power stroke defined by X-ray crystallography and EPR spectroscopy", Nature structural biology, 9, 844-8
- Yeon JH et al. (2016) "Systems-wide Identification of cis-Regulatory Elements in Proteins", Cell systems, 2, 89-100
Autoinhibited structure
Activated structure
1 structures for P33175
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-P33175-F1 | Predicted | AlphaFoldDB |
54 variants for P33175
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs3389104329 | 23 | E>V | No | EVA | |
| rs3389131086 | 37 | D>N | No | EVA | |
| rs3389071016 | 39 | S>N | No | EVA | |
| rs3389120442 | 65 | A>T | No | EVA | |
| rs3389127993 | 70 | I>V | No | EVA | |
| rs3389141192 | 108 | I>N | No | EVA | |
| rs3389123715 | 184 | I>S | No | EVA | |
| rs3389129798 | 186 | E>G | No | EVA | |
| rs3389071047 | 209 | F>L | No | EVA | |
| rs3389141206 | 215 | Q>* | No | EVA | |
| rs3412846132 | 238 | K>N | No | EVA | |
| rs3389129649 | 247 | A>G | No | EVA | |
| rs3389096653 | 302 | I>V | No | EVA | |
| rs3548449075 | 312 | A>T | No | EVA | |
| rs33857632 | 355 | A>T | No | EVA | |
| rs3389131043 | 456 | L>R | No | EVA | |
| rs3389131103 | 458 | S>P | No | EVA | |
| rs3389141753 | 460 | R>Q | No | EVA | |
| rs3389109133 | 463 | N>K | No | EVA | |
| rs3401551972 | 526 | M>RGFEVRC* | No | EVA | |
| rs3401748107 | 535 | R>SAFAGVH* | No | EVA | |
| rs3401414784 | 538 | E>G | No | EVA | |
| rs262744864 | 556 | R>K | No | EVA | |
| rs3389071105 | 562 | S>R | No | EVA | |
| rs3389132917 | 567 | N>K | No | EVA | |
| rs3389141145 | 575 | E>* | No | EVA | |
| rs3389096742 | 582 | E>Q | No | EVA | |
| rs3389137249 | 624 | R>M | No | EVA | |
| rs3389134581 | 629 | C>S | No | EVA | |
| rs3389131014 | 634 | S>L | No | EVA | |
| rs3401552034 | 641 | R>C | No | EVA | |
| rs3389128025 | 649 | T>* | No | EVA | |
| rs3389096687 | 649 | T>S | No | EVA | |
| rs3389071034 | 662 | D>Y | No | EVA | |
| rs3389104305 | 692 | A>T | No | EVA | |
| rs3389141143 | 703 | M>L | No | EVA | |
| rs3389129656 | 706 | H>Q | No | EVA | |
| rs3389129859 | 710 | H>Y | No | EVA | |
| rs3389123751 | 732 | K>N | No | EVA | |
| rs3389096737 | 766 | L>M | No | EVA | |
| rs3389129785 | 824 | H>L | No | EVA | |
| rs3389128006 | 838 | E>V | No | EVA | |
| rs3389129773 | 868 | T>M | No | EVA | |
| rs3389132864 | 896 | E>K | No | EVA | |
| rs3389129818 | 907 | K>N | No | EVA | |
| rs3389141715 | 909 | S>F | No | EVA | |
| rs3548358947 | 939 | R>Q | No | EVA | |
| rs3389123703 | 948 | N>I | No | EVA | |
| rs49422622 | 966 | T>A | No | EVA | |
| rs216030226 | 976 | A>V | No | EVA | |
| rs3389132857 | 982 | L>S | No | EVA | |
| rs3401764371 | 985 | Y>* | No | EVA | |
| rs3389109137 | 989 | N>I | No | EVA | |
| rs3401160995 | 1003 | S>G | No | EVA |
No associated diseases with P33175
Functions
15 GO annotations of cellular component
| Name | Definition |
|---|---|
| apical dendrite | A dendrite that emerges near the apical pole of a neuron. In bipolar neurons, apical dendrites are located on the opposite side of the soma from the axon. |
| axon | The long process of a neuron that conducts nerve impulses, usually away from the cell body to the terminals and varicosities, which are sites of storage and release of neurotransmitter. |
| axon cytoplasm | Any cytoplasm that is part of a axon. |
| central region of growth cone | The center of the migrating motile tip of a growing nerve cell axon or dendrite. |
| ciliary rootlet | A cytoskeleton-like structure, originating from the basal body at the proximal end of a cilium, and extending proximally toward the cell nucleus. Rootlets are typically 80-100 nm in diameter and contain cross striae distributed at regular intervals of approximately 55-70 nm. |
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| dendrite | A neuron projection that has a short, tapering, morphology. Dendrites receive and integrate signals from other neurons or from sensory stimuli, and conduct nerve impulses towards the axon or the cell body. In most neurons, the impulse is conveyed from dendrites to axon via the cell body, but in some types of unipolar neuron, the impulse does not travel via the cell body. |
| dendrite cytoplasm | All of the contents of a dendrite, excluding the surrounding plasma membrane. |
| kinesin complex | Any complex that includes a dimer of molecules from the kinesin superfamily, a group of related proteins that contain an extended region of predicted alpha-helical coiled coil in the main chain that likely produces dimerization. The native complexes of several kinesin family members have also been shown to contain additional peptides, often designated light chains as all of the noncatalytic subunits that are currently known are smaller than the chain that contains the motor unit. Kinesin complexes generally possess a force-generating enzymatic activity, or motor, which converts the free energy of the gamma phosphate bond of ATP into mechanical work. |
| microtubule | Any of the long, generally straight, hollow tubes of internal diameter 12-15 nm and external diameter 24 nm found in a wide variety of eukaryotic cells; each consists (usually) of 13 protofilaments of polymeric tubulin, staggered in such a manner that the tubulin monomers are arranged in a helical pattern on the microtubular surface, and with the alpha/beta axes of the tubulin subunits parallel to the long axis of the tubule; exist in equilibrium with pool of tubulin monomers and can be rapidly assembled or disassembled in response to physiological stimuli; concerned with force generation, e.g. in the spindle. |
| neuron projection | A prolongation or process extending from a nerve cell, e.g. an axon or dendrite. |
| neuronal cell body | The portion of a neuron that includes the nucleus, but excludes cell projections such as axons and dendrites. |
| P-body | A focus in the cytoplasm where mRNAs may become inactivated by decapping or some other mechanism. Protein and RNA localized to these foci are involved in mRNA degradation, nonsense-mediated mRNA decay (NMD), translational repression, and RNA-mediated gene silencing. |
| perikaryon | The portion of the cell soma (neuronal cell body) that excludes the nucleus. |
| perinuclear region of cytoplasm | Cytoplasm situated near, or occurring around, the nucleus. |
8 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| ATP hydrolysis activity | Catalysis of the reaction |
| kinesin binding | Interacting selectively and non-covalently and stoichiometrically with kinesin, a member of a superfamily of microtubule-based motor proteins that perform force-generating tasks such as organelle transport and chromosome segregation. |
| microtubule binding | Binding to a microtubule, a filament composed of tubulin monomers. |
| microtubule motor activity | A motor activity that generates movement along a microtubule, driven by ATP hydrolysis. |
| plus-end-directed microtubule motor activity | A motor activity that generates movement along a microtubule toward the plus end, driven by ATP hydrolysis. |
| protein-containing complex binding | Binding to a macromolecular complex. |
| scaffold protein binding | Binding to a scaffold protein. Scaffold proteins are crucial regulators of many key signaling pathways. Although not strictly defined in function, they are known to interact and/or bind with multiple members of a signaling pathway, tethering them into complexes. |
7 GO annotations of biological process
| Name | Definition |
|---|---|
| anterograde axonal protein transport | The directed movement of proteins along microtubules from the cell body toward the cell periphery in nerve cell axons. |
| anterograde dendritic transport of neurotransmitter receptor complex | The directed movement of a neurotransmitter receptor complex along microtubules in nerve cell dendrites towards the postsynapse. |
| axon guidance | The chemotaxis process that directs the migration of an axon growth cone to a specific target site in response to a combination of attractive and repulsive cues. |
| mitotic cell cycle | Progression through the phases of the mitotic cell cycle, the most common eukaryotic cell cycle, which canonically comprises four successive phases called G1, S, G2, and M and includes replication of the genome and the subsequent segregation of chromosomes into daughter cells. In some variant cell cycles nuclear replication or nuclear division may not be followed by cell division, or G1 and G2 phases may be absent. |
| retrograde neuronal dense core vesicle transport | The directed movement of neuronal dense core vesicles along axonal microtubules towards the cell body. |
| synaptic vesicle transport | The directed movement of synaptic vesicles. |
| vesicle-mediated transport | A cellular transport process in which transported substances are moved in membrane-bounded vesicles; transported substances are enclosed in the vesicle lumen or located in the vesicle membrane. The process begins with a step that directs a substance to the forming vesicle, and includes vesicle budding and coating. Vesicles are then targeted to, and fuse with, an acceptor membrane. |
16 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| P17210 | Khc | Kinesin heavy chain | Drosophila melanogaster (Fruit fly) | EV |
| O60282 | KIF5C | Kinesin heavy chain isoform 5C | Homo sapiens (Human) | EV |
| P33176 | KIF5B | Kinesin-1 heavy chain | Homo sapiens (Human) | EV |
| Q12840 | KIF5A | Kinesin heavy chain isoform 5A | Homo sapiens (Human) | EV |
| P28738 | Kif5c | Kinesin heavy chain isoform 5C | Mus musculus (Mouse) | SS |
| Q61768 | Kif5b | Kinesin-1 heavy chain | Mus musculus (Mouse) | EV |
| Q99PW8 | Kif17 | Kinesin-like protein KIF17 | Mus musculus (Mouse) | SS |
| Q7M6Z4 | Kif27 | Kinesin-like protein KIF27 | Mus musculus (Mouse) | SS |
| Q9QXL2 | Kif21a | Kinesin-like protein KIF21A | Mus musculus (Mouse) | EV SS |
| Q9QXL1 | Kif21b | Kinesin-like protein KIF21B | Mus musculus (Mouse) | SS |
| Q9EQW7 | Kif13a | Kinesin-like protein KIF13A | Mus musculus (Mouse) | SS |
| B7ZNG0 | Kif7 | Kinesin-like protein KIF7 | Mus musculus (Mouse) | SS |
| Q2PQA9 | Kif5b | Kinesin-1 heavy chain | Rattus norvegicus (Rat) | SS |
| Q6QLM7 | Kif5a | Kinesin heavy chain isoform 5A | Rattus norvegicus (Rat) | SS |
| P34540 | unc-116 | Kinesin heavy chain | Caenorhabditis elegans | SS |
| Q9SV36 | KINUC | Kinesin-like protein KIN-UC | Arabidopsis thaliana (Mouse-ear cress) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MAETNNECSI | KVLCRFRPLN | QAEILRGDKF | IPIFQGDDSV | IIGGKPYVFD | RVFPPNTTQE |
| 70 | 80 | 90 | 100 | 110 | 120 |
| QVYHACAMQI | VKDVLAGYNG | TIFAYGQTSS | GKTHTMEGKL | HDPQLMGIIP | RIARDIFNHI |
| 130 | 140 | 150 | 160 | 170 | 180 |
| YSMDENLEFH | IKVSYFEIYL | DKIRDLLDVT | KTNLSVHEDK | NRVPFVKGCT | ERFVSSPEEI |
| 190 | 200 | 210 | 220 | 230 | 240 |
| LDVIDEGKSN | RHVAVTNMNE | HSSRSHSIFL | INIKQENVET | EQKLSGKLYL | VDLAGSEKVS |
| 250 | 260 | 270 | 280 | 290 | 300 |
| KTGAEGAVLD | EAKNINKSLS | ALGNVISALA | EGTKSYVPYR | DSKMTRILQD | SLGGNCRTTM |
| 310 | 320 | 330 | 340 | 350 | 360 |
| FICCSPSSYN | DAETKSTLMF | GQRAKTIKNT | ASVNLELTAE | QWKKKYEKEK | EKTKAQKETI |
| 370 | 380 | 390 | 400 | 410 | 420 |
| AKLEAELSRW | RNGENVPETE | RLAGEDSALG | AELCEETPVN | DNSSIVVRIA | PEERQKYEEE |
| 430 | 440 | 450 | 460 | 470 | 480 |
| IRRLYKQLDD | KDDEINQQSQ | LIEKLKQQML | DQEELLVSTR | GDNEKVQREL | SHLQSENDAA |
| 490 | 500 | 510 | 520 | 530 | 540 |
| KDEVKEVLQA | LEELAVNYDQ | KSQEVEEKSQ | QNQLLVDELS | QKVATMLSLE | SELQRLQEVS |
| 550 | 560 | 570 | 580 | 590 | 600 |
| GHQRKRIAEV | LNGLMRDLSE | FSVIVGNGEI | KLPVEISGAI | EEEFTVARLY | ISKIKSEVKS |
| 610 | 620 | 630 | 640 | 650 | 660 |
| VVKRCRQLEN | LQVECHRKME | VTGRELSSCQ | LLISQHEAKI | RSLTEYMQTV | ELKKRHLEES |
| 670 | 680 | 690 | 700 | 710 | 720 |
| YDSLSDELAR | LQAHETVHEV | ALKDKEPDTQ | DAEEVKKALE | LQMENHREAH | HRQLARLRDE |
| 730 | 740 | 750 | 760 | 770 | 780 |
| INEKQKTIDE | LKDLNQKLQL | ELEKLQADYE | RLKNEENEKS | AKLQELTFLY | ERHEQSKQDL |
| 790 | 800 | 810 | 820 | 830 | 840 |
| KGLEETVARE | LQTLHNLRKL | FVQDVTTRVK | KSAEMEPEDS | GGIHSQKQKI | SFLENNLEQL |
| 850 | 860 | 870 | 880 | 890 | 900 |
| TKVHKQLVRD | NADLRCELPK | LEKRLRATAE | RVKALEGALK | EAKEGAMKDK | RRYQQEVDRI |
| 910 | 920 | 930 | 940 | 950 | 960 |
| KEAVRYKSSG | KRGHSAQIAK | PVRPGHYPAS | SPTNPYGTRS | PECISYTNNL | FQNYQNLHLQ |
| 970 | 980 | 990 | 1000 | 1010 | 1020 |
| AAPSSTSDMY | FASSGATSVA | PLASYQKANM | DNGNATDIND | NRSDLPCGYE | AEDQAKLFPL |
| HQETAAS |