P32944

PR
Gene name
SWE1 (YJL187C, J0406)
Protein name
Mitosis inhibitor protein kinase SWE1
Names
ICAM-3, CDw50, ICAM-R, Wee1 homolog
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
KEGG Pathway
sce:YJL187C
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

212-450 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

596-618 (Activation loop from InterPro)

Target domain

443-791 (Catalytic domain of the Protein Tyrosine Kinases, Fungal Wee1 proteins)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for P32944

Entry ID Method Resolution Chain Position Source
AF-P32944-F1 Predicted AlphaFoldDB

12 variants for P32944

Variant ID(s) Position Change Description Diseaes Association Provenance
s10-79173 30 Q>R No SGRP
s10-79137 42 G>E No SGRP
s10-78873 130 K>R No SGRP
s10-78834 143 R>Q No SGRP
s10-78632 210 K>N No SGRP
s10-78536 242 L>F No SGRP
s10-78405 286 T>S No SGRP
s10-78214 350 T>P No SGRP
s10-78067 399 A>S No SGRP
s10-77202 687 I>T No SGRP
s10-77139 708 N>T No SGRP
s10-77127 712 T>I No SGRP

No associated diseases with P32944

3 regional properties for P32944

Type Name Position InterPro Accession
domain Protein kinase domain 444 - 794 IPR000719
active_site Serine/threonine-protein kinase, active site 575 - 587 IPR008271
binding_site Protein kinase, ATP binding site 450 - 473 IPR017441

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
  • Bud neck
  • Nucleus
  • When SWE1 first accumulates in G1, it is localized to the nucleus
  • After bud emergence, a subpopulation is recruited to the daughter side of the mother-bud neck through HSL1 and its adapter HSL7, where it is susceptible to hyperphosphorylation and degradation
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
cellular bud neck The constriction between the mother cell and daughter cell (bud) in an organism that reproduces by budding.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

6 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
metal ion binding Binding to a metal ion.
protein kinase activity Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
protein tyrosine kinase activity Catalysis of the reaction: ATP + a protein tyrosine = ADP + protein tyrosine phosphate.

10 GO annotations of biological process

Name Definition
cell division The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells.
G2/M transition of mitotic cell cycle The mitotic cell cycle transition by which a cell in G2 commits to M phase. The process begins when the kinase activity of M cyclin/CDK complex reaches a threshold high enough for the cell cycle to proceed. This is accomplished by activating a positive feedback loop that results in the accumulation of unphosphorylated and active M cyclin/CDK complex.
meiotic cell cycle Progression through the phases of the meiotic cell cycle, in which canonically a cell replicates to produce four offspring with half the chromosomal content of the progenitor cell via two nuclear divisions.
mitotic morphogenesis checkpoint signaling A signaling process that contributes to a mitotic cell cycle checkpoint which delays mitotic onset in response to perturbations that affect cell shape via the actin cytoskeleton, septin organization, small cell size, and/or the extent of membrane growth.
negative regulation of mitotic spindle pole body separation Any process that decreases the rate, frequency or extent of the process involving the release of duplicated mitotic spindle pole bodies (SPBs) and their migration away from each other within the nuclear membrane.
positive regulation of sphingolipid biosynthetic process Any process that increases the rate, frequency or extent of sphingolipid biosynthesis. Sphingolipid biosynthesis is the chemical reactions and pathways resulting in the formation of sphingolipids, any of a class of lipids containing the long-chain amine diol sphingosine or a closely related base (a sphingoid).
re-entry into mitotic cell cycle The resumption of the mitotic cell division cycle by cells that were in a quiescent or other non-dividing state.
regulation of cell size Any process that modulates the size of a cell.
regulation of cyclin-dependent protein serine/threonine kinase activity Any process that modulates the frequency, rate or extent of cyclin-dependent protein serine/threonine kinase activity.
regulation of meiotic nuclear division Any process that modulates the frequency, rate or extent of meiotic nuclear division, the process in which the nucleus of a diploid cell divides twice forming four haploid cells, one or more of which usually function as gametes.

2 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P15442 GCN2 eIF-2-alpha kinase GCN2 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) EV
Q99640 PKMYT1 Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase Homo sapiens (Human) PR
10 20 30 40 50 60
MSSLDEDEED FEMLDTENLQ FMGKKMFGKQ AGEDESDDFA IGGSTPTNKL KFYPYSNNKL
70 80 90 100 110 120
TRSTGTLNLS LSNTALSEAN SKFLGKIEEE EEEEEEGKDE ESVDSRIKRW SPFHENESVT
130 140 150 160 170 180
TPITKRSAEK TNSPISLKQW NQRWFPKNDA RTENTSSSSS YSVAKPNQSA FTSSGLVSKM
190 200 210 220 230 240
SMDTSLYPAK LRIPETPVKK SPLVEGRDHK HVHLSSSKNA SSSLSVSPLN FVEDNNLQED
250 260 270 280 290 300
LLFSDSPSSK ALPSIHVPTI DSSPLSEAKY HAHDRHNNQT NILSPTNSLV TNSSPQTLHS
310 320 330 340 350 360
NKFKKIKRAR NSVILKNREL TNSLQQFKDD LYGTDENFPP PIIISSHHST RKNPQPYQFR
370 380 390 400 410 420
GRYDNDTDEE ISTPTRRKSI IGATSQTHRE SRPLSLSSAI VTNTTSAETH SISSTDSSPL
430 440 450 460 470 480
NSKRRLISSN KLSANPDSHL FEKFTNVHSI GKGQFSTVYQ VTFAQTNKKY AIKAIKPNKY
490 500 510 520 530 540
NSLKRILLEI KILNEVTNQI TMDQEGKEYI IDYISSWKFQ NSYYIMTELC ENGNLDGFLQ
550 560 570 580 590 600
EQVIAKKKRL EDWRIWKIIV ELSLALRFIH DSCHIVHLDL KPANVMITFE GNLKLGDFGM
610 620 630 640 650 660
ATHLPLEDKS FENEGDREYI APEIISDCTY DYKADIFSLG LMIVEIAANV VLPDNGNAWH
670 680 690 700 710 720
KLRSGDLSDA GRLSSTDIHS ESLFSDITKV DTNDLFDFER DNISGNSNNA GTSTVHNNSN
730 740 750 760 770 780
INNPNMNNGN DNNNVNTAAT KNRLILHKSS KIPAWVPKFL IDGESLERIV RWMIEPNYER
790 800 810
RPTANQILQT EECLYVEMTR NAGAIIQEDD FGPKPKFFI